ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 1 | 4 | 0 | 2 | 2 |
Bud | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 8 | 3 | 3 | 4 | 5 | 1 | 1 | 0 | – | – | 0 | 0 | 1 | 2 | 5 | 11 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | – | – | 0 | 0 | 1 | 2 | 1 | 5 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | – | – | 0 | 0 | 0 | 0 | 1 | 2 | 2 |
Cytoplasm | 64 | 231 | 280 | 252 | 283 | 255 | 355 | 393 | 361 | 420 | 293 | 383 | 412 | 412 | 383 | – | – | 107 | 37 | 105 | 79 | 157 | 247 | 248 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | – | – | 8 | 0 | 0 | 5 | 3 | 5 | 6 |
Endosome | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | 2 | 2 | 3 | 7 | 3 | 6 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 2 | 2 | 1 | 2 |
Mitochondria | 5 | 18 | 9 | 8 | 18 | 59 | 98 | 86 | 101 | 158 | 83 | 134 | 0 | 0 | 0 | – | – | 9 | 4 | 8 | 18 | 11 | 25 | 34 |
Nucleus | 0 | 0 | 0 | 2 | 8 | 15 | 40 | 36 | 20 | 41 | 12 | 25 | 3 | 3 | 1 | – | – | 25 | 0 | 0 | 1 | 2 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 0 | 2 | 5 | 4 | 6 | 4 | 2 | 2 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 2 | 1 | 4 | 12 |
Vac/Vac Membrane | 1 | 3 | 8 | 6 | 97 | 93 | 162 | 120 | 138 | 181 | 101 | 153 | 1 | 2 | 0 | – | – | 103 | 47 | 105 | 148 | 162 | 249 | 289 |
Unique Cell Count | 68 | 239 | 290 | 261 | 350 | 334 | 514 | 541 | 495 | 617 | 396 | 539 | 417 | 417 | 384 | 190 | 96 | 234 | 284 | 365 | 577 | 652 | ||
Labelled Cell Count | 70 | 255 | 299 | 269 | 412 | 429 | 666 | 652 | 631 | 809 | 496 | 705 | 426 | 428 | 389 | 254 | 96 | 234 | 284 | 365 | 577 | 652 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 3.3 | 3.7 | 3.6 | 4.0 | 3.6 | 3.5 | 3.9 | 3.8 | 3.5 | 3.7 | 3.6 | 5.0 | 5.0 | 5.2 | 3.9 | 4.7 | 5.7 | 5.3 | 5.1 | 5.4 |
Std Deviation (1e-4) | 0.3 | 0.5 | 1.3 | 1.4 | 1.5 | 1.6 | 0.8 | 1.4 | 1.2 | 1.1 | 1.2 | 1.1 | 1.1 | 1.1 | 1.4 | 1.1 | 1.3 | 1.4 | 1.6 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.09 | -0.04 | -0.08 | 0.04 | 0.01 | -0.11 | -0.02 | -0.07 | 0.42 | 0.42 | 0.48 | 0.07 | 0.33 | 0.6 | 0.51 | 0.46 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 13.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1787 | 0.1366 | 0.1002 | -0.0728 | 0.0092 | 0.1748 | -1.6154 | -0.5479 | -1.122 | -1.1781 | -1.2088 | -0.9087 | -1.7095 | -0.7446 | -1.4037 | -1.5955 | -1.7758 | -1.3988 |
Actin | 0.019 | 0.0047 | 0.0137 | 0.0003 | 0.0234 | 0.001 | 0.0159 | 0.0006 | 0.0002 | 0.0002 | 0.0114 | 0.004 | 0.0535 | 0.0001 | 0.0112 | 0 | 0 | 0.0028 |
Bud | 0.0024 | 0.0084 | 0.0027 | 0.0008 | 0.0083 | 0.0015 | 0.0001 | 0.0019 | 0.0005 | 0.0017 | 0.0002 | 0.0011 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0002 |
Bud Neck | 0.0045 | 0.0012 | 0.0021 | 0.0005 | 0.0041 | 0.0046 | 0.0013 | 0.0003 | 0.0051 | 0.0013 | 0.0004 | 0.0022 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0005 |
Bud Periphery | 0.0047 | 0.0125 | 0.0026 | 0.0019 | 0.0045 | 0.0034 | 0.0002 | 0.0015 | 0.0017 | 0.0034 | 0.0005 | 0.003 | 0.0006 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0004 |
Bud Site | 0.0104 | 0.0149 | 0.0035 | 0.0009 | 0.0044 | 0.0007 | 0.0006 | 0.0118 | 0.0024 | 0.0025 | 0.0006 | 0.0013 | 0.002 | 0.0014 | 0.0043 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0007 | 0.001 | 0.0006 | 0.0002 | 0.0014 | 0.0004 | 0.0008 | 0.0004 | 0.0007 | 0.0006 | 0.0004 | 0.0005 | 0.0005 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.1038 | 0.1443 | 0.0988 | 0.1287 | 0.1056 | 0.1656 | 0.0609 | 0.2071 | 0.1597 | 0.1422 | 0.2458 | 0.18 | 0.1244 | 0.2213 | 0.1554 | 0.205 | 0.1482 | 0.2593 |
Cytoplasmic Foci | 0.0535 | 0.0522 | 0.0639 | 0.0837 | 0.0581 | 0.0492 | 0.0199 | 0.0325 | 0.0292 | 0.1099 | 0.031 | 0.043 | 0.0323 | 0.0418 | 0.0534 | 0.0586 | 0.1501 | 0.0402 |
Eisosomes | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0014 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0076 | 0.0028 | 0.0028 | 0.0031 | 0.0045 | 0.0028 | 0.0019 | 0.0066 | 0.0033 | 0.0029 | 0.0038 | 0.0013 | 0.0047 | 0.0049 | 0.0054 | 0.0032 | 0.0066 | 0.0023 |
Endosome | 0.0885 | 0.0675 | 0.0943 | 0.1171 | 0.0961 | 0.0816 | 0.0373 | 0.0259 | 0.0447 | 0.0935 | 0.0669 | 0.0687 | 0.0309 | 0.1008 | 0.1204 | 0.1104 | 0.1301 | 0.0628 |
Golgi | 0.0046 | 0.0026 | 0.0034 | 0.0046 | 0.0099 | 0.0017 | 0.0014 | 0.0007 | 0.0008 | 0.0024 | 0.0112 | 0.0016 | 0.0046 | 0.0014 | 0.0024 | 0.0013 | 0.0015 | 0.0009 |
Lipid Particles | 0.0143 | 0.0046 | 0.0115 | 0.022 | 0.021 | 0.0105 | 0.0124 | 0.0051 | 0.0033 | 0.0102 | 0.0763 | 0.0016 | 0.0091 | 0.0047 | 0.0133 | 0.0068 | 0.0091 | 0.0036 |
Mitochondria | 0.0057 | 0.0059 | 0.0058 | 0.0048 | 0.0302 | 0.011 | 0.001 | 0.0014 | 0.0061 | 0.0037 | 0.0272 | 0.0062 | 0.036 | 0.0022 | 0.0131 | 0.0017 | 0.0047 | 0.0106 |
None | 0.5357 | 0.5571 | 0.5735 | 0.4905 | 0.43 | 0.5367 | 0.806 | 0.6788 | 0.6563 | 0.4899 | 0.4309 | 0.5852 | 0.6613 | 0.4982 | 0.5295 | 0.5718 | 0.4939 | 0.5332 |
Nuclear Periphery | 0.0189 | 0.0019 | 0.0036 | 0.0033 | 0.0147 | 0.0034 | 0.0012 | 0.0017 | 0.0028 | 0.002 | 0.0025 | 0.0007 | 0.0016 | 0.0036 | 0.0043 | 0.0032 | 0.0106 | 0.0015 |
Nucleolus | 0.0038 | 0.0051 | 0.0042 | 0.0033 | 0.0055 | 0.0016 | 0.0015 | 0.0007 | 0.0014 | 0.0014 | 0.0007 | 0.0006 | 0.0005 | 0.0016 | 0.0013 | 0.0006 | 0.0006 | 0.0003 |
Nucleus | 0.0148 | 0.0106 | 0.0088 | 0.0076 | 0.0107 | 0.0099 | 0.002 | 0.0015 | 0.0036 | 0.0048 | 0.0025 | 0.0021 | 0.001 | 0.0033 | 0.0032 | 0.0019 | 0.0032 | 0.0016 |
Peroxisomes | 0.0074 | 0.0026 | 0.008 | 0.0102 | 0.022 | 0.0057 | 0.0034 | 0.0007 | 0.0012 | 0.0045 | 0.0151 | 0.003 | 0.0078 | 0.0007 | 0.0028 | 0.0006 | 0.001 | 0.0004 |
Punctate Nuclear | 0.0062 | 0.0025 | 0.0041 | 0.0039 | 0.0059 | 0.006 | 0.0036 | 0.0023 | 0.0013 | 0.0113 | 0.0018 | 0.0005 | 0.0008 | 0.001 | 0.0013 | 0.001 | 0.0058 | 0.0014 |
Vacuole | 0.0837 | 0.0914 | 0.0829 | 0.1024 | 0.1285 | 0.0938 | 0.0266 | 0.0168 | 0.0716 | 0.1032 | 0.065 | 0.0837 | 0.0251 | 0.0975 | 0.0683 | 0.0294 | 0.0286 | 0.0705 |
Vacuole Periphery | 0.0094 | 0.006 | 0.0089 | 0.0103 | 0.011 | 0.009 | 0.0014 | 0.0016 | 0.0039 | 0.0082 | 0.0042 | 0.0094 | 0.0015 | 0.0136 | 0.0085 | 0.0038 | 0.0048 | 0.0072 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.2559 | 7.1366 | 7.5652 | 13.2687 | 14.7867 | 13.1081 | 12.4988 | 14.6105 | 19.0664 | 18.2506 |
Translational Efficiency | 1.7333 | 1.565 | 1.3984 | 0.802 | 0.876 | 2.339 | 1.0667 | 0.9551 | 1.0093 | 1.0623 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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