Standard name | |
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Human Ortholog | |
Description | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 1 | 2 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 4 | 2 |
Bud | 1 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 3 | 1 | 7 | 2 | 1 | 3 | 7 | 2 | 3 | 9 | 7 |
Bud Neck | 0 | 2 | – | – | – | – | – | – | – | – | – | – | 0 | 3 | 1 | 3 | 3 | 2 | 1 | 5 | 0 | 1 | 4 | 4 |
Bud Site | 3 | 3 | – | – | – | – | – | – | – | – | – | – | 4 | 4 | 3 | 8 | 9 | 10 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 8 | – | – | – | – | – | – | – | – | – | – | 5 | 15 | 18 | 41 | 32 | 11 | 1 | 0 | 1 | 2 | 11 | 8 |
Endoplasmic Reticulum | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 3 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 3 | 8 | – | – | – | – | – | – | – | – | – | – | 3 | 18 | 7 | 46 | 19 | 29 | 4 | 3 | 0 | 4 | 4 | 3 |
Golgi | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 |
Mitochondria | 7 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 4 | 1 | 7 | 2 | 5 | 1 | 0 | 0 | 0 | 4 | 4 |
Nucleus | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 7 | 4 | – | – | – | – | – | – | – | – | – | – | 1 | 6 | 6 | 7 | 9 | 29 | 1 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 11 | 13 | – | – | – | – | – | – | – | – | – | – | 9 | 19 | 12 | 18 | 18 | 12 | 1 | 1 | 0 | 0 | 2 | 1 |
SpindlePole | 37 | 56 | – | – | – | – | – | – | – | – | – | – | 17 | 58 | 49 | 191 | 123 | 135 | 61 | 77 | 7 | 29 | 69 | 60 |
Vac/Vac Membrane | 1 | 2 | – | – | – | – | – | – | – | – | – | – | 1 | 4 | 1 | 15 | 5 | 10 | 0 | 0 | 0 | 0 | 4 | 1 |
Unique Cell Count | 56 | 83 | 33 | 96 | 80 | 271 | 177 | 172 | 81 | 102 | 14 | 47 | 119 | 109 | ||||||||||
Labelled Cell Count | 76 | 100 | 43 | 137 | 106 | 349 | 224 | 252 | 81 | 102 | 14 | 47 | 119 | 109 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.4 | – | – | – | – | – | – | – | – | – | – | 5.8 | 5.9 | 6.2 | 7.3 | 7.8 | 7.2 | – | 5.0 | 6.5 |
Std Deviation (1e-4) | 1.1 | 1.0 | – | – | – | – | – | – | – | – | – | – | 1.9 | 2.4 | 2.9 | 1.9 | 2.4 | 1.4 | – | 1.5 | 3.6 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.3175 | 15.5274 | 2.3044 | 5.4228 | 17.4534 | 13.02 | 15.3072 | 11.5234 | 6.7757 | 21.5396 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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