Standard name
Human Ortholog
Description Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.08 0.18 0.07 0.08 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Bud Site 0.05 0 0.12 0 0 0 0.05 0.06
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.1 0.15 0.16 0.22 0.15 0.18 0.06 0 0 0.12 0 0.09 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0.1 0.09 0.19 0.09 0.17 0.11 0.17 0.05 0 0 0.09 0 0
Golgi 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05
Mitochondria 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.05 0 0.06 0.07 0 0.05 0.17 0 0 0 0 0 0
Peroxisomes 0.2 0.16 0.27 0.2 0.15 0.07 0.1 0.07 0 0 0.05 0 0 0
SpindlePole 0.66 0.67 0.52 0.6 0.61 0.7 0.69 0.78 0.76 0.76 0.53 0.63 0.59 0.56
Vac/Vac Membrane 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 2 2 0 2 0 1 0 0 1 4 2
Bud 1 0 0 3 1 7 2 1 3 7 2 3 9 7
Bud Neck 0 2 0 3 1 3 3 2 1 5 0 1 4 4
Bud Site 3 3 4 4 3 8 9 10
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 8 5 15 18 41 32 11 1 0 1 2 11 8
Endoplasmic Reticulum 0 1 0 0 3 5 0 3 0 0 0 0 1 1
Endosome 3 8 3 18 7 46 19 29 4 3 0 4 4 3
Golgi 0 0 2 1 0 1 0 0 0 1 0 0 0 5
Mitochondria 7 1 0 4 1 7 2 5 1 0 0 0 4 4
Nucleus 0 1 0 0 1 0 0 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 7 4 1 6 6 7 9 29 1 1 0 0 0 0
Peroxisomes 11 13 9 19 12 18 18 12 1 1 0 0 2 1
SpindlePole 37 56 17 58 49 191 123 135 61 77 7 29 69 60
Vac/Vac Membrane 1 2 1 4 1 15 5 10 0 0 0 0 4 1
Unique Cell Count 56 83 33 96 80 271 177 172 81 102 14 47 119 109
Labelled Cell Count 76 100 43 137 106 349 224 252 81 102 14 47 119 109


Punctate Nuclear, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.4 5.8 5.9 6.2 7.3 7.8 7.2 5.0 6.5
Std Deviation (1e-4) 1.1 1.0 1.9 2.4 2.9 1.9 2.4 1.4 1.5 3.6
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.3175 15.5274 2.3044 5.4228 17.4534 13.02 15.3072 11.5234 6.7757 21.5396
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
Localization
Cell Percentages spindle pole (84%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc98

Spc98


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc98-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available