Standard name
Human Ortholog
Description Nicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.96 0.95 0.85 0.82 0.78 – 0.69 0.7 0.63 0.67 0.98 0.99 1.0 0.89 0.9 0.94 0.96 0.94 0.92 0.91 0.85 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0 – 0.21 0.17 0.25 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.19 0.06 0.21 – 0.16 0.15 0.18 0.14 0 0 0 0.12 0.13 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 0 – 1 0 0 0 0 0 0 0 2 0 1 2 2 1 1 2
Bud 0 0 0 0 0 4 0 – 2 5 6 7 0 0 0 0 0 0 4 0 1 2 3 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 4 2 4 2 6 – 5 3 2 2 2 0 3 7 12 5 0 0 0 0 0 0
Cytoplasm 401 451 279 225 182 256 320 – 229 309 257 300 204 252 292 257 260 224 386 430 264 174 203 172
Endoplasmic Reticulum 1 6 1 4 1 2 7 – 0 2 4 6 0 1 0 13 5 4 0 2 2 0 5 7
Endosome 0 0 2 0 1 1 3 – 2 10 16 9 0 0 0 13 5 4 0 4 1 0 10 13
Golgi 1 2 0 0 0 0 0 – 0 0 0 0 1 0 0 2 1 2 1 2 2 1 0 4
Mitochondria 0 3 1 1 2 50 12 – 69 77 101 99 0 0 0 2 0 0 0 2 2 3 2 2
Nucleus 2 0 0 3 3 3 10 – 6 12 8 5 3 1 2 2 3 2 1 3 2 2 1 2
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 2 0 2 1 1 0 1 6 0 0 1 1 0 1 8
Vac/Vac Membrane 7 2 10 8 40 18 85 – 53 66 74 63 1 1 1 36 39 17 0 3 3 2 1 7
Unique Cell Count 408 463 291 237 215 312 410 331 441 409 450 208 255 293 288 289 239 402 456 288 193 240 236
Labelled Cell Count 412 466 297 243 234 337 445 367 486 468 497 212 256 298 333 334 259 402 456 288 193 240 236


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 8.3 8.9 9.2 9.4 6.3 7.6 – 6.4 6.4 6.1 6.0 9.2 9.1 8.8 10.3 11.1 10.1 8.0 8.1 8.3
Std Deviation (1e-4) 1.5 1.6 1.6 1.7 1.6 1.6 1.5 – 1.4 1.3 1.0 1.1 1.6 1.7 1.8 1.9 1.8 2.0 1.8 1.9 2.2
Intensity Change (Log2) – – – 0.05 0.08 -0.5 -0.23 – -0.48 -0.49 -0.55 -0.58 0.05 0.03 -0.01 0.21 0.32 0.18 -0.16 -0.14 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0.9 2.0 0
Cytoplasm -0.5 -4.4 -5.4 -6.6 – -8.6 -8.6 -10.2 -9.4 1.4 2.1 3.1 -3.0 -2.8 -1.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.3 0 0
Endosome 0 0 0 0 – 0 0 2.7 0 0 0 0 2.9 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.9 2.5 – 8.1 7.3 9.0 8.4 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.7 1.4 6.6 – 5.2 5.0 5.9 4.7 0 -2.5 -2.8 4.0 4.4 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6219 1.8887 0.961 1.4106 1.3819 1.6205 2.189 3.5389 2.8218 3.6948 3.1674 3.1894 5.2034 7.2721 5.5616 4.7223 5.6382 5.7968
Actin 0.0098 0.0062 0.0211 0.0226 0.0027 0.001 0.0046 0.0008 0.0037 0.0334 0.0425 0.0018 0.0014 0.0012 0.0094 0.0004 0.0005 0.0066
Bud 0.0006 0.0006 0.0016 0.001 0.0007 0.0002 0.0003 0.0014 0.0004 0.0006 0.0028 0.0099 0.0001 0.0006 0.0012 0.0001 0.0003 0.0001
Bud Neck 0.003 0.0004 0.0007 0.0076 0.0012 0.0011 0.0003 0.0002 0.0005 0.0008 0.0003 0.0317 0.0002 0.0002 0.0033 0.0002 0.0003 0.001
Bud Periphery 0.001 0.0006 0.0011 0.0018 0.0008 0.0001 0.0003 0.0006 0.0003 0.0005 0.0016 0.0054 0.0001 0.0003 0.0013 0 0.0002 0
Bud Site 0.0015 0.0113 0.0098 0.005 0.0017 0.0001 0.0007 0.0073 0.0016 0.0014 0.001 0.0004 0.0002 0.0019 0.0008 0.0001 0.0002 0.0001
Cell Periphery 0.0002 0.0002 0.0002 0.0006 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0 0 0
Cytoplasm 0.6068 0.778 0.7044 0.6775 0.5961 0.8658 0.6414 0.8762 0.7134 0.841 0.5036 0.7303 0.7318 0.8757 0.7777 0.9572 0.7881 0.8481
Cytoplasmic Foci 0.0407 0.0118 0.0225 0.0349 0.0783 0.0117 0.0135 0.0037 0.0162 0.0163 0.0573 0.0088 0.0052 0.0043 0.0101 0.0043 0.0074 0.0108
Eisosomes 0.0005 0 0.0001 0.0004 0 0 0.0001 0 0 0.0002 0.0001 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0088 0.0027 0.0069 0.0016 0.0029 0.0042 0.0035 0.0018 0.008 0.001 0.013 0.0026 0.004 0.0012 0.0022 0.0018 0.0031 0.0023
Endosome 0.0379 0.0095 0.014 0.0415 0.0591 0.0136 0.0436 0.0009 0.0076 0.0147 0.1482 0.0259 0.0032 0.001 0.0027 0.0012 0.0047 0.0045
Golgi 0.0083 0.0041 0.0086 0.0286 0.0218 0.0017 0.0189 0.0001 0.0089 0.0271 0.054 0.0049 0.0002 0.0002 0.0007 0.0002 0.0005 0.0005
Lipid Particles 0.0126 0.0028 0.0032 0.0137 0.0045 0.0003 0.0032 0 0.0005 0.0051 0.0019 0.0072 0.0004 0.0001 0.0003 0 0.0001 0.0004
Mitochondria 0.0086 0.0005 0.0038 0.0666 0.0106 0.0014 0.0054 0.0001 0.0006 0.0108 0.0239 0.0109 0.0002 0.0002 0.0015 0.0001 0.0004 0.0002
None 0.2402 0.163 0.1876 0.0771 0.1109 0.0913 0.2562 0.1038 0.2316 0.0354 0.1137 0.1447 0.2444 0.1088 0.1775 0.0316 0.1856 0.1122
Nuclear Periphery 0.0056 0.0008 0.002 0.0009 0.0427 0.0012 0.0018 0.0005 0.0009 0.0006 0.0035 0.0023 0.0019 0.0004 0.0011 0.0005 0.001 0.0015
Nucleolus 0.0005 0.0001 0.0001 0.0002 0.0009 0.0001 0.0001 0 0.0001 0.0001 0.0007 0.0002 0.0001 0 0.0001 0 0 0.0001
Nucleus 0.0037 0.0021 0.0022 0.0025 0.0132 0.0034 0.0017 0.0014 0.0016 0.0023 0.0037 0.0038 0.002 0.0022 0.0034 0.0015 0.0057 0.0087
Peroxisomes 0.0025 0.002 0.0037 0.008 0.0427 0.0003 0.001 0.0001 0.0013 0.0061 0.0007 0.0037 0.0002 0.0005 0.0046 0 0.0001 0.0003
Punctate Nuclear 0.0022 0.0017 0.0042 0.0015 0.0037 0.0009 0.0008 0.0003 0.0011 0.0006 0.0184 0.0007 0.0031 0.0003 0.0009 0.0002 0.0004 0.0018
Vacuole 0.0036 0.0012 0.0018 0.0037 0.0031 0.0013 0.0018 0.0006 0.0013 0.0015 0.0052 0.0032 0.001 0.0007 0.0009 0.0004 0.0012 0.0006
Vacuole Periphery 0.0015 0.0001 0.0004 0.0026 0.0023 0.0005 0.0007 0.0001 0.0002 0.0005 0.0036 0.0014 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.3138 19.1 19.2348 21.0465 29.6807 18.3036 25.5194 22.81 22.3553 29.2907
Translational Efficiency 1.8235 1.3341 1.1217 1.3199 0.8782 1.7425 1.1812 0.9586 1.2207 1.2527

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nrk1

Nrk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nrk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available