Standard name | |
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Human Ortholog | |
Description | Nicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 2 | 1 | 1 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 4 | 0 | – | 2 | 5 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 4 | 2 | 4 | 2 | 6 | – | 5 | 3 | 2 | 2 | 2 | 0 | 3 | 7 | 12 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 401 | 451 | 279 | 225 | 182 | 256 | 320 | – | 229 | 309 | 257 | 300 | 204 | 252 | 292 | 257 | 260 | 224 | 386 | 430 | 264 | 174 | 203 | 172 |
Endoplasmic Reticulum | 1 | 6 | 1 | 4 | 1 | 2 | 7 | – | 0 | 2 | 4 | 6 | 0 | 1 | 0 | 13 | 5 | 4 | 0 | 2 | 2 | 0 | 5 | 7 |
Endosome | 0 | 0 | 2 | 0 | 1 | 1 | 3 | – | 2 | 10 | 16 | 9 | 0 | 0 | 0 | 13 | 5 | 4 | 0 | 4 | 1 | 0 | 10 | 13 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 2 | 1 | 0 | 4 |
Mitochondria | 0 | 3 | 1 | 1 | 2 | 50 | 12 | – | 69 | 77 | 101 | 99 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 2 |
Nucleus | 2 | 0 | 0 | 3 | 3 | 3 | 10 | – | 6 | 12 | 8 | 5 | 3 | 1 | 2 | 2 | 3 | 2 | 1 | 3 | 2 | 2 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 2 | 1 | 1 | 0 | 1 | 6 | 0 | 0 | 1 | 1 | 0 | 1 | 8 |
Vac/Vac Membrane | 7 | 2 | 10 | 8 | 40 | 18 | 85 | – | 53 | 66 | 74 | 63 | 1 | 1 | 1 | 36 | 39 | 17 | 0 | 3 | 3 | 2 | 1 | 7 |
Unique Cell Count | 408 | 463 | 291 | 237 | 215 | 312 | 410 | 331 | 441 | 409 | 450 | 208 | 255 | 293 | 288 | 289 | 239 | 402 | 456 | 288 | 193 | 240 | 236 | |
Labelled Cell Count | 412 | 466 | 297 | 243 | 234 | 337 | 445 | 367 | 486 | 468 | 497 | 212 | 256 | 298 | 333 | 334 | 259 | 402 | 456 | 288 | 193 | 240 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 8.3 | 8.9 | 9.2 | 9.4 | 6.3 | 7.6 | – | 6.4 | 6.4 | 6.1 | 6.0 | 9.2 | 9.1 | 8.8 | 10.3 | 11.1 | 10.1 | 8.0 | 8.1 | 8.3 |
Std Deviation (1e-4) | 1.5 | 1.6 | 1.6 | 1.7 | 1.6 | 1.6 | 1.5 | – | 1.4 | 1.3 | 1.0 | 1.1 | 1.6 | 1.7 | 1.8 | 1.9 | 1.8 | 2.0 | 1.8 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.08 | -0.5 | -0.23 | – | -0.48 | -0.49 | -0.55 | -0.58 | 0.05 | 0.03 | -0.01 | 0.21 | 0.32 | 0.18 | -0.16 | -0.14 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 2.0 | 0 |
Cytoplasm | -0.5 | -4.4 | -5.4 | -6.6 | – | -8.6 | -8.6 | -10.2 | -9.4 | 1.4 | 2.1 | 3.1 | -3.0 | -2.8 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.9 | 2.5 | – | 8.1 | 7.3 | 9.0 | 8.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 5.7 | 1.4 | 6.6 | – | 5.2 | 5.0 | 5.9 | 4.7 | 0 | -2.5 | -2.8 | 4.0 | 4.4 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6219 | 1.8887 | 0.961 | 1.4106 | 1.3819 | 1.6205 | 2.189 | 3.5389 | 2.8218 | 3.6948 | 3.1674 | 3.1894 | 5.2034 | 7.2721 | 5.5616 | 4.7223 | 5.6382 | 5.7968 |
Actin | 0.0098 | 0.0062 | 0.0211 | 0.0226 | 0.0027 | 0.001 | 0.0046 | 0.0008 | 0.0037 | 0.0334 | 0.0425 | 0.0018 | 0.0014 | 0.0012 | 0.0094 | 0.0004 | 0.0005 | 0.0066 |
Bud | 0.0006 | 0.0006 | 0.0016 | 0.001 | 0.0007 | 0.0002 | 0.0003 | 0.0014 | 0.0004 | 0.0006 | 0.0028 | 0.0099 | 0.0001 | 0.0006 | 0.0012 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.003 | 0.0004 | 0.0007 | 0.0076 | 0.0012 | 0.0011 | 0.0003 | 0.0002 | 0.0005 | 0.0008 | 0.0003 | 0.0317 | 0.0002 | 0.0002 | 0.0033 | 0.0002 | 0.0003 | 0.001 |
Bud Periphery | 0.001 | 0.0006 | 0.0011 | 0.0018 | 0.0008 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0005 | 0.0016 | 0.0054 | 0.0001 | 0.0003 | 0.0013 | 0 | 0.0002 | 0 |
Bud Site | 0.0015 | 0.0113 | 0.0098 | 0.005 | 0.0017 | 0.0001 | 0.0007 | 0.0073 | 0.0016 | 0.0014 | 0.001 | 0.0004 | 0.0002 | 0.0019 | 0.0008 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.6068 | 0.778 | 0.7044 | 0.6775 | 0.5961 | 0.8658 | 0.6414 | 0.8762 | 0.7134 | 0.841 | 0.5036 | 0.7303 | 0.7318 | 0.8757 | 0.7777 | 0.9572 | 0.7881 | 0.8481 |
Cytoplasmic Foci | 0.0407 | 0.0118 | 0.0225 | 0.0349 | 0.0783 | 0.0117 | 0.0135 | 0.0037 | 0.0162 | 0.0163 | 0.0573 | 0.0088 | 0.0052 | 0.0043 | 0.0101 | 0.0043 | 0.0074 | 0.0108 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0088 | 0.0027 | 0.0069 | 0.0016 | 0.0029 | 0.0042 | 0.0035 | 0.0018 | 0.008 | 0.001 | 0.013 | 0.0026 | 0.004 | 0.0012 | 0.0022 | 0.0018 | 0.0031 | 0.0023 |
Endosome | 0.0379 | 0.0095 | 0.014 | 0.0415 | 0.0591 | 0.0136 | 0.0436 | 0.0009 | 0.0076 | 0.0147 | 0.1482 | 0.0259 | 0.0032 | 0.001 | 0.0027 | 0.0012 | 0.0047 | 0.0045 |
Golgi | 0.0083 | 0.0041 | 0.0086 | 0.0286 | 0.0218 | 0.0017 | 0.0189 | 0.0001 | 0.0089 | 0.0271 | 0.054 | 0.0049 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0005 |
Lipid Particles | 0.0126 | 0.0028 | 0.0032 | 0.0137 | 0.0045 | 0.0003 | 0.0032 | 0 | 0.0005 | 0.0051 | 0.0019 | 0.0072 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0004 |
Mitochondria | 0.0086 | 0.0005 | 0.0038 | 0.0666 | 0.0106 | 0.0014 | 0.0054 | 0.0001 | 0.0006 | 0.0108 | 0.0239 | 0.0109 | 0.0002 | 0.0002 | 0.0015 | 0.0001 | 0.0004 | 0.0002 |
None | 0.2402 | 0.163 | 0.1876 | 0.0771 | 0.1109 | 0.0913 | 0.2562 | 0.1038 | 0.2316 | 0.0354 | 0.1137 | 0.1447 | 0.2444 | 0.1088 | 0.1775 | 0.0316 | 0.1856 | 0.1122 |
Nuclear Periphery | 0.0056 | 0.0008 | 0.002 | 0.0009 | 0.0427 | 0.0012 | 0.0018 | 0.0005 | 0.0009 | 0.0006 | 0.0035 | 0.0023 | 0.0019 | 0.0004 | 0.0011 | 0.0005 | 0.001 | 0.0015 |
Nucleolus | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0037 | 0.0021 | 0.0022 | 0.0025 | 0.0132 | 0.0034 | 0.0017 | 0.0014 | 0.0016 | 0.0023 | 0.0037 | 0.0038 | 0.002 | 0.0022 | 0.0034 | 0.0015 | 0.0057 | 0.0087 |
Peroxisomes | 0.0025 | 0.002 | 0.0037 | 0.008 | 0.0427 | 0.0003 | 0.001 | 0.0001 | 0.0013 | 0.0061 | 0.0007 | 0.0037 | 0.0002 | 0.0005 | 0.0046 | 0 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0022 | 0.0017 | 0.0042 | 0.0015 | 0.0037 | 0.0009 | 0.0008 | 0.0003 | 0.0011 | 0.0006 | 0.0184 | 0.0007 | 0.0031 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0018 |
Vacuole | 0.0036 | 0.0012 | 0.0018 | 0.0037 | 0.0031 | 0.0013 | 0.0018 | 0.0006 | 0.0013 | 0.0015 | 0.0052 | 0.0032 | 0.001 | 0.0007 | 0.0009 | 0.0004 | 0.0012 | 0.0006 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0004 | 0.0026 | 0.0023 | 0.0005 | 0.0007 | 0.0001 | 0.0002 | 0.0005 | 0.0036 | 0.0014 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.3138 | 19.1 | 19.2348 | 21.0465 | 29.6807 | 18.3036 | 25.5194 | 22.81 | 22.3553 | 29.2907 |
Translational Efficiency | 1.8235 | 1.3341 | 1.1217 | 1.3199 | 0.8782 | 1.7425 | 1.1812 | 0.9586 | 1.2207 | 1.2527 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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