Standard name | |
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Human Ortholog | |
Description | Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 0 | 0 | 5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 4 | 3 | 4 | 6 | – | 4 | 7 | 8 | 8 | 15 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 127 | 118 | 314 | 294 | 231 | 290 | – | 327 | 243 | 318 | 239 | 281 | 57 | 46 | 95 | – | 49 | 39 | 135 | 117 | 306 | 30 | 81 | 160 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 2 | 4 | – | 6 | 5 | 4 | 10 | 13 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | 2 | 1 | 0 | 15 | – | 48 | 48 | 95 | 80 | 90 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 2 | 0 | 1 | 7 | – | 15 | 13 | 13 | 15 | 11 | 2 | 1 | 3 | – | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 4 | 0 | 5 | 3 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 1 | 9 | 16 | 41 | 25 | – | 153 | 67 | 82 | 23 | 60 | 0 | 0 | 1 | – | 5 | 0 | 2 | 2 | 0 | 0 | 3 | 0 |
Unique Cell Count | 132 | 120 | 324 | 309 | 263 | 332 | 514 | 345 | 457 | 337 | 424 | 57 | 46 | 96 | 52 | 39 | 142 | 125 | 316 | 33 | 89 | 168 | ||
Labelled Cell Count | 136 | 120 | 332 | 315 | 280 | 348 | 562 | 386 | 527 | 378 | 477 | 60 | 48 | 99 | 61 | 40 | 142 | 125 | 316 | 33 | 89 | 168 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.4 | 9.7 | 13.1 | 12.8 | 11.3 | 9.5 | – | 8.3 | 8.0 | 7.5 | 7.6 | 7.7 | 13.6 | 13.3 | 13.1 | – | 11.2 | 13.1 | – | 15.5 | 16.2 |
Std Deviation (1e-4) | 2.4 | 1.8 | 1.9 | 1.9 | 1.8 | 1.5 | – | 1.5 | 1.5 | 1.4 | 1.5 | 1.4 | 2.2 | 1.4 | 1.6 | – | 1.7 | 1.4 | – | 2.1 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.21 | -0.47 | – | -0.66 | -0.71 | -0.8 | -0.8 | -0.77 | 0.05 | 0.02 | -0.01 | – | -0.23 | -0.0 | – | 0.24 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0.8 | 0 | 1.1 | 2.0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | -1.1 | -4.2 | -4.5 | – | -11.1 | -9.2 | -9.6 | -9.0 | -10.3 | 1.3 | 1.2 | 1.1 | – | -1.0 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.7 | 2.8 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 3.1 | – | 5.2 | 6.5 | 8.4 | 9.0 | 8.5 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 2.3 | 2.8 | 2.2 | 3.1 | 2.1 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 1.5 | 5.5 | 2.7 | – | 9.6 | 6.8 | 6.5 | 2.4 | 5.3 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.1443 | 11.8922 | 11.0755 | 10.559 | 9.7763 | 10.7974 | 8.7172 | 12.7017 | 11.1086 | 9.9444 | 10.3315 | 10.6843 | 9.2569 | 12.6549 | 11.6633 | 12.0154 | 10.0517 | 11.562 |
Actin | 0.0033 | 0.0004 | 0.0003 | 0.0105 | 0.0003 | 0.0013 | 0.0184 | 0.0002 | 0.0007 | 0.0004 | 0.0275 | 0.0142 | 0.0066 | 0.0012 | 0.0005 | 0.0005 | 0.0005 | 0.0003 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0019 | 0 | 0.0013 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0008 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0013 | 0.0077 | 0.0001 | 0.0003 | 0.0004 | 0.0011 | 0.0011 | 0.0015 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0009 |
Bud Periphery | 0.0001 | 0 | 0 | 0.0008 | 0 | 0.0006 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0005 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8971 | 0.977 | 0.9748 | 0.9423 | 0.9502 | 0.9361 | 0.8422 | 0.9873 | 0.9555 | 0.9866 | 0.9241 | 0.9536 | 0.9158 | 0.9926 | 0.9882 | 0.9901 | 0.9856 | 0.9851 |
Cytoplasmic Foci | 0.0177 | 0.0012 | 0.0009 | 0.0042 | 0.0015 | 0.0031 | 0.0299 | 0.001 | 0.0083 | 0.001 | 0.008 | 0.0034 | 0.0091 | 0.0008 | 0.0006 | 0.0007 | 0.0017 | 0.0008 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0048 | 0.0008 | 0.0009 | 0.0014 | 0.0016 | 0.0019 | 0.0062 | 0.0005 | 0.0004 | 0.0012 | 0.0003 | 0.0007 | 0.0034 | 0.0006 | 0.0005 | 0.0005 | 0.0012 | 0.0006 |
Endosome | 0.0106 | 0.0003 | 0.0003 | 0.0009 | 0.0008 | 0.0012 | 0.015 | 0.0002 | 0.0036 | 0.0003 | 0.0017 | 0.0017 | 0.0083 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 |
Golgi | 0.0014 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0067 | 0 | 0.0067 | 0 | 0.0058 | 0.0011 | 0.0016 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0031 | 0 | 0 | 0.0001 | 0 | 0 | 0.0291 | 0 | 0.0024 | 0 | 0.0035 | 0.0002 | 0.0024 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0035 | 0 | 0.004 | 0.0001 | 0.0007 | 0.0038 | 0.0015 | 0 | 0 | 0 | 0.0001 | 0 |
None | 0.0068 | 0.0016 | 0.0016 | 0.006 | 0.0013 | 0.0043 | 0.003 | 0.0009 | 0.0014 | 0.0011 | 0.0007 | 0.001 | 0.0232 | 0.0009 | 0.0008 | 0.0008 | 0.0007 | 0.0006 |
Nuclear Periphery | 0.0093 | 0.0011 | 0.0016 | 0.0043 | 0.0031 | 0.0039 | 0.0105 | 0.0005 | 0.0005 | 0.0008 | 0.0007 | 0.0009 | 0.0066 | 0.0003 | 0.0005 | 0.0005 | 0.0011 | 0.0007 |
Nucleolus | 0.0024 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0271 | 0.016 | 0.0183 | 0.0207 | 0.0393 | 0.0412 | 0.0088 | 0.0086 | 0.0057 | 0.0075 | 0.0094 | 0.0148 | 0.0072 | 0.0027 | 0.008 | 0.0059 | 0.0071 | 0.0104 |
Peroxisomes | 0.0049 | 0 | 0 | 0.001 | 0 | 0.0001 | 0.0118 | 0 | 0.0092 | 0 | 0.0152 | 0.0025 | 0.0026 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.007 | 0.0004 | 0.0004 | 0.004 | 0.0008 | 0.0022 | 0.0028 | 0.0003 | 0.0005 | 0.0002 | 0.0005 | 0.0004 | 0.0064 | 0.0003 | 0.0002 | 0.0002 | 0.0006 | 0.0003 |
Vacuole | 0.0013 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 79.0067 | 58.9061 | 46.8331 | 73.9104 | 68.8716 | 61.1543 | 66.701 | 67.8869 | 60.9164 | 72.9617 |
Translational Efficiency | 1.2357 | 1.4695 | 1.5855 | 1.0197 | 1.1882 | 1.7261 | 1.1804 | 1.1108 | 1.261 | 1.1766 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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