Standard name
Human Ortholog
Description Protein with a role in the ubiquitin-proteasome pathway; interacts with ubiquitinated protein, Cdc48p and the proteasomal regulatory particle; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element and is co-regulated with proteasome subunit genes; AN1-type zinc finger protein, with DHHC and ubiquitin-like domains (UBL); ortholog of ZFAND1, a human gene linked to cancer; protein abundance increases under DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.85 0.9 0.76 0.81 0.79 0.84 0.72 0.79 0.69 0.64 0.93 0.94 0.96 0.84 0.77 0.79 0.74 0.82 0.84 0.85 0.85 0.84
Endoplasmic Reticulum 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.05 0.06 0.43 0.24 0.4 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0.12 0.17 0.14 0.05 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.1 0.06 0.21 0.05 0.13 0.1 0.07 0 0 0 0.07 0.06 0.06 0.12 0.19 0.17 0.11 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 2 1 2 0 2
Bud 2 0 1 0 4 0 0 4 6 5 4 0 0 0 0 0 0 2 3 1 6 6 10
Bud Neck 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 3 11 0 9 8 14
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 0 1 1 2 6 5 1 9 0 3 9 13 19 4 5 11 0 0 0 0 0 0
Cytoplasm 138 312 98 111 108 168 266 205 220 148 104 396 495 546 596 350 421 204 357 125 412 354 382
Endoplasmic Reticulum 1 22 5 7 4 5 3 2 1 0 4 10 15 2 9 5 8 4 6 4 3 5 11
Endosome 6 0 0 1 0 0 2 1 2 2 0 0 0 2 3 2 1 3 3 1 2 1 1
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 0 0 1 0
Mitochondria 5 8 0 1 11 11 20 121 68 86 59 0 0 1 9 0 2 2 5 3 4 2 0
Nucleus 6 11 3 6 6 11 10 3 1 3 7 5 17 3 82 78 75 14 18 1 13 10 10
Nuclear Periphery 5 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 3 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 1 1 1 3 1 2
Vac/Vac Membrane 53 36 6 30 7 28 31 19 12 7 7 32 29 36 85 85 89 29 21 4 20 16 9
Unique Cell Count 196 369 109 146 133 214 317 283 279 216 162 428 527 570 709 454 532 277 438 149 484 417 454
Labelled Cell Count 220 390 115 157 145 229 339 356 321 252 189 452 569 609 788 526 608 277 438 149 484 417 454


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.0 6.3 7.9 6.1 6.5 5.8 4.4 4.7 4.4 4.2 9.2 9.0 7.9 8.6 10.8 10.7 8.9 9.3 9.2
Std Deviation (1e-4) 1.2 1.0 1.1 1.2 1.7 1.1 1.1 1.0 1.2 1.1 0.7 1.4 1.3 1.4 1.6 2.2 2.3 1.7 1.6 1.5
Intensity Change (Log2) 0.33 -0.03 0.05 -0.11 -0.52 -0.4 -0.49 -0.58 0.56 0.52 0.33 0.46 0.79 0.78 0.51 0.57 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.9 -1.9 -2.5 -1.5 -3.7 -2.5 -4.2 -4.8 0.9 1.5 2.5 -1.6 -3.0 -2.6
Endoplasmic Reticulum 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 2.8 3.9 3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.4 -0.1 2.1 1.4 0.4 -0.5 0 -0.4 0.7 0 0.3 2.0 3.4 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4559 6.5966 6.4854 6.1022 5.9511 6.6761 4.9412 6.154 5.7454 5.7328 4.8738 5.4625 2.0657 3.6897 3.2767 2.7916 3.2332 3.0571
Actin 0.0505 0.0117 0.0192 0.0078 0.0396 0.0009 0.0281 0.0011 0.01 0.002 0.0008 0.0006 0.0038 0.0009 0.0215 0.0012 0.0089 0.0021
Bud 0.001 0.0002 0.0008 0.0002 0.0003 0.0001 0.0015 0.0001 0.0004 0.0001 0.0001 0.0001 0.0006 0.0002 0.0003 0.0001 0.0006 0.0002
Bud Neck 0.0029 0.0015 0.0027 0.0018 0.0033 0.003 0.0017 0.0009 0.0007 0.0008 0.0019 0.0031 0.0009 0.0005 0.0011 0.0017 0.0108 0.0035
Bud Periphery 0.0013 0.0002 0.0007 0.0002 0.0004 0.0001 0.0036 0 0.0006 0 0 0 0.0008 0.0001 0.0003 0 0.0008 0.0002
Bud Site 0.0053 0.0048 0.0025 0.0013 0.0007 0.0002 0.0032 0.0015 0.0015 0.0003 0.0001 0.0002 0.0118 0.0022 0.005 0.0003 0.0095 0.0005
Cell Periphery 0.0004 0.0007 0.0004 0.0005 0.0003 0.0001 0.0008 0.0002 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0003 0.0001 0.0004 0.0001
Cytoplasm 0.7531 0.8584 0.8404 0.7748 0.7409 0.881 0.7568 0.8395 0.7391 0.8479 0.7947 0.8503 0.6519 0.9289 0.7968 0.8889 0.6923 0.8717
Cytoplasmic Foci 0.0158 0.0087 0.0086 0.0068 0.0159 0.0035 0.0115 0.0032 0.0065 0.0037 0.0027 0.0042 0.0381 0.0021 0.0073 0.0024 0.0144 0.0063
Eisosomes 0.0003 0.0007 0.0001 0.0005 0.0005 0 0.0003 0 0.0001 0 0 0 0.0004 0 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.0152 0.0055 0.0098 0.0101 0.0082 0.009 0.0277 0.0191 0.0102 0.0154 0.0056 0.0071 0.0145 0.0051 0.0118 0.0056 0.0064 0.0075
Endosome 0.024 0.002 0.0038 0.0024 0.0045 0.0043 0.027 0.0013 0.0073 0.0024 0.001 0.0028 0.0285 0.0006 0.0046 0.0007 0.0233 0.0042
Golgi 0.008 0.0005 0.0017 0.0024 0.0035 0.0054 0.0054 0.0001 0.0065 0.0002 0.0001 0.0002 0.0037 0.0001 0.0074 0.0001 0.0306 0.003
Lipid Particles 0.0082 0.0004 0.0012 0.0037 0.0028 0.0008 0.0031 0.0002 0.0012 0.0002 0.0001 0.0001 0.0103 0.0001 0.005 0.0001 0.0421 0.0015
Mitochondria 0.0032 0.0002 0.002 0.017 0.0015 0.0017 0.0046 0.0001 0.0211 0.0003 0.0002 0.0003 0.0445 0.0001 0.0048 0.0001 0.0636 0.0045
None 0.0622 0.0534 0.057 0.1173 0.1451 0.0457 0.0709 0.0534 0.1428 0.0929 0.131 0.0634 0.0742 0.0322 0.0714 0.0434 0.0072 0.0397
Nuclear Periphery 0.0216 0.0026 0.0043 0.0034 0.0024 0.003 0.0126 0.0032 0.0039 0.0076 0.0024 0.0028 0.0086 0.0021 0.0071 0.0029 0.0052 0.0028
Nucleolus 0.0003 0.0002 0.0003 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0049 0.0001 0.0001 0.0001 0.0034 0.0001
Nucleus 0.0105 0.0442 0.0301 0.0248 0.0136 0.0377 0.0178 0.071 0.0435 0.022 0.0551 0.0616 0.0587 0.0218 0.0456 0.0481 0.0457 0.0467
Peroxisomes 0.009 0.0005 0.0048 0.0214 0.0123 0.0001 0.0013 0.0001 0.0009 0.0001 0 0.0002 0.0323 0.0001 0.0043 0 0.0067 0.0027
Punctate Nuclear 0.0036 0.0024 0.008 0.0026 0.0033 0.0025 0.0044 0.0038 0.0022 0.0024 0.0039 0.0019 0.0071 0.002 0.0042 0.0037 0.0239 0.0015
Vacuole 0.0024 0.0011 0.0014 0.0007 0.0006 0.0008 0.0161 0.001 0.0008 0.0013 0.0003 0.0006 0.0024 0.0005 0.0007 0.0004 0.003 0.0011
Vacuole Periphery 0.0013 0.0001 0.0002 0.0001 0.0002 0.0001 0.0014 0 0.0002 0.0001 0 0.0001 0.0013 0 0.0002 0.0001 0.001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.4427 38.2594 37.2621 42.296 33.8145 24.061 45.0307 48.1387 44.5064 33.8465
Translational Efficiency 1.4393 1.7388 1.5386 1.4976 1.2733 2.7584 1.3475 2.004 1.7298 1.364

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein with a role in the ubiquitin-proteasome pathway; interacts with ubiquitinated protein, Cdc48p and the proteasomal regulatory particle; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element and is co-regulated with proteasome subunit genes; AN1-type zinc finger protein, with DHHC and ubiquitin-like domains (UBL); ortholog of ZFAND1, a human gene linked to cancer; protein abundance increases under DNA replication stress
Localization
Cell Percentages cytoplasm (55%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cuz1

Cuz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cuz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available