Standard name
Human Ortholog
Description Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.06 0 0.05 0.1 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.05 0 0.06 0.05 – 0 0.05 0.09 0.07 0.06 0.11 0.16 0.22 0.14 0 0 0 0 0 0 0
Cytoplasm 0.05 0.15 0 0.09 0.08 0.21 0.1 – 0.13 0.13 0.11 0.13 0.12 0.12 0.1 0.22 0.36 0.18 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.89 0.83 0.84 0.88 0.71 0.61 0.55 – 0.54 0.51 0.57 0.55 0.79 0.73 0.81 0 0 0 0.95 0.85 0.92 0.89 0.89 0.87
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0.15 0.21 0.15 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.17 0.14 0.12 0.15 0 0 0 0.11 0.12 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0.05 0.06 0 0.05 0.07 – 0.11 0.08 0 0.06 0 0.05 0.05 0.06 0.06 0.05 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.06 0.19 0.05 0.26 0.16 0.29 – 0.18 0.24 0.19 0.16 0.12 0.13 0.08 0.29 0.18 0.46 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 5 5 5 4 12 – 16 11 14 37 13 15 13 7 4 6 1 2 2 4 2 4
Bud 0 0 1 0 0 0 0 – 0 4 3 2 0 1 0 0 0 0 0 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 13 6 8 10 13 – 9 14 25 26 25 53 87 26 16 3 0 0 0 1 2 3
Cytoplasm 8 24 9 11 18 36 26 – 35 37 33 48 47 57 57 26 40 17 0 11 2 8 6 12
Endoplasmic Reticulum 155 137 170 113 156 105 148 – 147 141 165 199 305 336 442 3 4 3 179 175 194 300 297 385
Endosome 0 0 1 0 2 1 1 – 1 1 0 0 1 1 1 1 0 3 0 0 0 1 1 1
Golgi 2 0 6 1 12 0 7 – 0 2 3 1 4 4 3 18 24 14 0 1 0 0 1 0
Mitochondria 1 1 0 1 1 4 10 – 45 39 34 55 3 2 0 13 13 6 0 1 5 7 4 9
Nucleus 2 0 0 0 1 0 3 – 2 7 2 1 1 0 0 3 1 5 0 0 0 0 0 0
Nuclear Periphery 12 5 11 8 7 8 20 – 29 23 7 20 15 22 25 7 7 5 2 0 2 1 2 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 26 10 38 6 57 28 79 – 49 68 55 56 48 60 46 34 20 43 3 8 1 8 11 22
Unique Cell Count 174 165 202 129 220 172 271 272 278 290 360 387 461 545 118 112 93 189 206 212 339 335 445
Labelled Cell Count 208 184 254 151 267 196 319 333 347 341 446 462 551 674 139 129 105 189 206 212 339 335 445


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 8.6 12.1 12.5 14.8 13.6 12.6 – 11.3 11.7 12.0 11.8 11.5 12.1 11.7 12.8 14.6 14.3 11.6 12.0 11.9
Std Deviation (1e-4) 2.0 1.6 2.0 2.1 2.8 3.4 2.5 – 2.8 2.8 2.9 2.7 2.0 2.0 2.0 3.3 3.7 4.4 1.9 1.9 1.9
Intensity Change (Log2) – – – 0.04 0.29 0.17 0.06 – -0.1 -0.05 -0.01 -0.04 -0.07 -0.0 -0.05 0.08 0.27 0.24 -0.07 -0.02 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.1 – 1.8 0.9 1.3 3.4 0.6 0.5 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -1.3 -0.2 -0.8 – -1.6 -0.7 0.9 0.4 0 2.0 3.4 4.1 2.3 -1.1
Cytoplasm 1.5 1.6 4.9 2.1 – 3.1 3.3 2.7 3.3 3.0 3.1 2.6 4.9 7.3 3.9
Endoplasmic Reticulum 0.9 -3.2 -5.0 -6.8 – -6.9 -7.6 -6.4 -6.9 -1.6 -3.1 -1.0 -14.1 -13.8 -13.1
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 1.3 0 -0.3 – 0 0 0 0 0 0 0 4.0 5.3 3.8
Mitochondria 0 0 0 0 – 6.1 5.6 5.0 5.8 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.3 -1.1 -0.3 0.8 – 2.0 1.2 -1.8 0.1 -0.9 -0.4 -0.5 0.2 0.3 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -3.7 1.7 -0.6 2.6 – -0.2 1.5 0 -1.0 -2.1 -1.9 -4.0 2.1 -0.2 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6246 9.3105 9.4479 9.0144 8.6689 9.1988 8.943 9.2635 8.8028 8.4598 7.6522 8.8651 7.0832 7.1181 6.6433 6.5987 6.4184 6.6113
Actin 0.0296 0.0001 0.0038 0.0175 0.0063 0.0003 0.0121 0.0002 0.0078 0 0.0001 0.0004 0.0183 0.0014 0.0008 0 0.0002 0.0025
Bud 0.0002 0 0.0001 0.0001 0.0005 0 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0.0001
Bud Neck 0.0003 0 0.0003 0.0003 0.0006 0.0007 0.0001 0.0002 0.0013 0 0.0001 0.0002 0.0116 0.0002 0.0014 0 0.0004 0.0008
Bud Periphery 0.0003 0 0.0002 0.0002 0.0007 0 0.0001 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0 0 0.0003
Bud Site 0.0006 0 0.0003 0.0001 0.0024 0 0.0001 0.0001 0.0003 0 0 0 0.0015 0.0023 0.0003 0 0.0001 0
Cell Periphery 0.0005 0.0004 0.0029 0.001 0.0003 0.0002 0.0003 0.0008 0.0005 0.0002 0.0003 0.0003 0.0005 0.0008 0.0026 0.0002 0.0002 0.0003
Cytoplasm 0.0258 0.0133 0.0393 0.018 0.0156 0.0248 0.0081 0.0328 0.0186 0.001 0.0097 0.0136 0.031 0.0729 0.0334 0.0129 0.0186 0.0284
Cytoplasmic Foci 0.0126 0.0005 0.0012 0.004 0.0144 0.0005 0.0019 0.0007 0.0028 0 0.0002 0.0012 0.0037 0.003 0.0012 0.0005 0.0015 0.001
Eisosomes 0.0003 0 0.0003 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.7607 0.9688 0.8947 0.904 0.8488 0.9436 0.9219 0.9144 0.9387 0.9925 0.9673 0.9233 0.8488 0.8767 0.9375 0.9612 0.9365 0.9302
Endosome 0.0299 0.0025 0.0082 0.0075 0.0255 0.0048 0.0067 0.0053 0.0069 0.0008 0.0036 0.0135 0.0103 0.0048 0.0026 0.0032 0.0141 0.0049
Golgi 0.0123 0.0003 0.0035 0.0051 0.0092 0.0002 0.0038 0.0002 0.0022 0.0001 0.0004 0.0011 0.0029 0.0011 0.0004 0.0001 0.0004 0.0021
Lipid Particles 0.0276 0.0019 0.0053 0.011 0.0094 0.0007 0.0042 0.0014 0.0052 0.0001 0.001 0.0026 0.0096 0.0056 0.0022 0.0014 0.0035 0.0017
Mitochondria 0.0193 0 0.0022 0.001 0.0064 0.0002 0.0006 0.0001 0.0005 0.0001 0.0003 0.0027 0.0014 0.0002 0.0001 0.0001 0.0002 0.0011
None 0.0071 0.0003 0.0117 0.0159 0.0078 0.0027 0.0007 0.0158 0.0007 0 0.0002 0.0087 0.005 0.0065 0.0012 0.0002 0.0003 0.0006
Nuclear Periphery 0.0437 0.0093 0.0181 0.0076 0.023 0.0156 0.0283 0.02 0.0095 0.004 0.0126 0.0249 0.0324 0.0163 0.012 0.0168 0.0135 0.0175
Nucleolus 0.0003 0 0.0001 0.0001 0.0003 0 0 0.0001 0.0001 0 0 0.0001 0.0009 0 0 0 0.0001 0.0001
Nucleus 0.0075 0.0005 0.0014 0.0005 0.0042 0.0019 0.0011 0.0029 0.0007 0.0001 0.0011 0.0019 0.0101 0.0027 0.0011 0.0011 0.0025 0.0035
Peroxisomes 0.0107 0 0.0002 0.0007 0.0063 0 0.007 0 0.0005 0 0 0.0001 0.0034 0.0001 0 0 0 0.0009
Punctate Nuclear 0.0051 0.0001 0.0016 0.0013 0.0144 0.0001 0.0006 0.0002 0.0012 0 0.0001 0.0007 0.0039 0.0005 0.0003 0 0.0009 0.0011
Vacuole 0.0034 0.0017 0.0035 0.0035 0.0028 0.0032 0.0015 0.0043 0.0018 0.0009 0.0025 0.0034 0.0031 0.0044 0.0024 0.0019 0.0066 0.0026
Vacuole Periphery 0.0024 0.0002 0.0012 0.0004 0.0012 0.0005 0.0007 0.0005 0.0005 0.0002 0.0004 0.0012 0.0008 0.0004 0.0002 0.0003 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.2631 30.6873 37.4099 48.2989 20.6644 44.1117 44.0485 39.2657 51.1433 39.8905
Translational Efficiency 0.9045 0.8743 0.7812 0.714 1.1853 0.8921 0.7333 1.1673 0.7951 0.789

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Nsg2

Nsg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available