Standard name
Human Ortholog
Description Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, a guanine nucleotide exchange factor (GEF), promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Bud – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Bud Neck – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Bud Site – 0 – – – – – – – – – – – – – 0 0 0 – – – – – –
Cell Periphery – 0 – – – – – – – – – – – – – 0.06 0.08 0.07 – 0 – – – –
Cytoplasm – 0.94 – – – – – – – – – – – – – 0.89 0.88 0.89 – 0.84 – – – –
Endoplasmic Reticulum – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Endosome – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Golgi – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Mitochondria – 0.24 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Nucleus – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Nuclear Periphery – 0.06 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Nucleolus – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Peroxisomes – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
SpindlePole – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Vac/Vac Membrane – 0 – – – – – – – – – – – – – 0.13 0.13 0.11 – 0.05 – – – –
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – – – – – – – – – – – – – 0 1 3 – 0 – – – –
Bud – 0 – – – – – – – – – – – – – 0 1 0 – 1 – – – –
Bud Neck – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Bud Site – 0 – – – – – – – – – – – – – 0 0 0 – – – – – –
Cell Periphery – 0 – – – – – – – – – – – – – 71 91 83 – 0 – – – –
Cytoplasm – 16 – – – – – – – – – – – – – 985 965 1033 – 45 – – – –
Endoplasmic Reticulum – 0 – – – – – – – – – – – – – 39 46 46 – 1 – – – –
Endosome – 0 – – – – – – – – – – – – – 2 2 0 – 1 – – – –
Golgi – 0 – – – – – – – – – – – – – 2 3 2 – 0 – – – –
Mitochondria – 4 – – – – – – – – – – – – – 27 4 13 – 0 – – – –
Nucleus – 0 – – – – – – – – – – – – – 7 7 6 – 0 – – – –
Nuclear Periphery – 1 – – – – – – – – – – – – – 0 2 1 – 0 – – – –
Nucleolus – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
Peroxisomes – 0 – – – – – – – – – – – – – 0 0 0 – 0 – – – –
SpindlePole – 0 – – – – – – – – – – – – – 1 0 0 – 0 – – – –
Vac/Vac Membrane – 0 – – – – – – – – – – – – – 145 146 124 – 2 – – – –
Unique Cell Count 17 1107 1100 1156 54
Labelled Cell Count 21 1279 1268 1311 54


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 6.3 – – – – – – – – – – – – – 8.7 9.1 9.0 – – –
Std Deviation (1e-4) – 1.0 – – – – – – – – – – – – – 0.9 1.0 1.2 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0239 0.0117 0.0161 0.0185 0.0005 0.0604 – – – – – – – – – – – –
Bud 0.0041 0.0105 0.0119 0.0192 0.0002 0.0008 – – – – – – – – – – – –
Bud Neck 0.0462 0.0008 0.003 0.0021 0.0006 0.0018 – – – – – – – – – – – –
Bud Periphery 0.0089 0.0045 0.0133 0.031 0 0.0005 – – – – – – – – – – – –
Bud Site 0.119 0.0254 0.0227 0.0034 0.0001 0.0006 – – – – – – – – – – – –
Cell Periphery 0.0096 0.0011 0.0007 0.0007 0.0001 0.0002 – – – – – – – – – – – –
Cytoplasm 0.1672 0.8519 0.7799 0.7742 0.9187 0.7724 – – – – – – – – – – – –
Cytoplasmic Foci 0.0301 0.0217 0.0228 0.0162 0.0285 0.028 – – – – – – – – – – – –
Eisosomes 0.0011 0.0002 0.0002 0.0002 0 0.0001 – – – – – – – – – – – –
Endoplasmic Reticulum 0.0722 0.0016 0.0053 0.0068 0.0067 0.0024 – – – – – – – – – – – –
Endosome 0.2211 0.017 0.038 0.0372 0.0325 0.0575 – – – – – – – – – – – –
Golgi 0.0289 0.003 0.0033 0.0019 0.0036 0.0521 – – – – – – – – – – – –
Lipid Particles 0.0168 0.0006 0.0003 0.0012 0.0001 0.0005 – – – – – – – – – – – –
Mitochondria 0.0836 0.0206 0.0548 0.0023 0.0003 0.0076 – – – – – – – – – – – –
None 0.006 0.0171 0.0056 0.0564 0.0035 0.0022 – – – – – – – – – – – –
Nuclear Periphery 0.0122 0.0007 0.0022 0.0024 0.0009 0.0006 – – – – – – – – – – – –
Nucleolus 0.0044 0.0002 0.0001 0.0011 0 0 – – – – – – – – – – – –
Nucleus 0.0406 0.0016 0.0018 0.0038 0.0006 0.0014 – – – – – – – – – – – –
Peroxisomes 0.0046 0.0011 0.0004 0.0022 0.0001 0.0006 – – – – – – – – – – – –
Punctate Nuclear 0.0013 0.0006 0.0005 0.0089 0.0002 0.0001 – – – – – – – – – – – –
Vacuole 0.0822 0.0069 0.0141 0.0081 0.0024 0.0072 – – – – – – – – – – – –
Vacuole Periphery 0.016 0.0011 0.0029 0.0022 0.0004 0.0032 – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3462 24.2673 19.9838 23.7492 18.4567 25.808 23.3397 23.932 23.2979 27.844
Translational Efficiency 1.2953 0.9951 1.16 0.8115 1.0095 0.7999 1.0624 0.6827 0.7635 0.7118

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ria1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available