Standard name
Human Ortholog
Description Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; regulated by phosphorylation and ubiquitination; likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity; necessary for oxidant and ramped heat stress-induced cell death; ortholog of mammalian RAC1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.51 0.85 0.76 0.42 0.24 0.24 0.12 0.12 – 0.08 0.09 0.06 – 0.68 0.63 0.66 0.66 0.71 0.27 0.33 0.4 0.36 0.31 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0.05 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.14 0.12 – 0.11 0.26 0.31 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.11 0.08 0.16 0.4 0.42 0.35 0.4 – 0.44 0.46 0.41 – 0 0.05 0.09 0.07 0.07 0.52 0.46 0.36 0.28 0.28 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0 – 0.08 0.1 0.11 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.21 0.4 0.56 0.46 0.27 0.37 0.36 – 0.33 0.19 0.25 – 0.44 0.46 0.4 0.42 0.41 0.14 0.13 0.14 0.23 0.28 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 – 0 1 0 – 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 2 4 2 – 3 2 5 – 0 0 0 0 0 1 4 0 2 4 6
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 – 2 5 5 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 – 0 0 0 – 0 0 0 3 0 0 0 0 0 0 0
Cytoplasm 23 93 47 69 60 42 32 46 – 20 21 17 – 133 142 216 209 193 20 42 31 44 60 83
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 – 0 0 0 – 2 3 16 11 10 0 1 1 1 6 7
Endosome 0 0 0 0 0 4 11 20 – 10 0 6 – 1 1 0 0 0 0 0 1 0 2 5
Golgi 1 1 0 2 2 0 0 0 – 0 0 0 – 0 0 13 12 8 0 0 0 0 1 0
Mitochondria 0 0 0 3 5 16 38 47 – 28 60 83 – 1 0 4 3 2 0 1 0 1 1 0
Nucleus 1 12 5 26 99 74 93 153 – 113 108 112 – 7 11 29 23 20 39 59 28 34 54 63
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 1 0 1 5
Nucleolus 0 0 0 0 3 3 12 15 – 21 24 31 – 1 0 0 0 0 0 0 0 3 1 3
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 25 23 25 92 115 48 97 140 – 83 44 67 – 86 105 130 133 110 11 17 11 28 54 67
Unique Cell Count 45 109 62 163 248 178 265 384 255 233 270 197 226 328 318 271 76 131 80 125 194 253
Labelled Cell Count 50 130 77 192 284 190 288 425 280 265 326 231 262 408 394 344 76 131 80 125 194 253


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.8 23.2 13.3 13.4 12.0 11.2 9.8 10.7 – 10.7 10.9 10.9 – 16.1 17.1 27.7 27.6 29.0 19.0 20.2 19.4
Std Deviation (1e-4) 6.4 4.8 3.5 2.9 2.4 3.4 2.2 2.3 – 3.1 3.3 3.4 – 3.2 3.6 7.6 7.0 7.8 4.7 5.3 3.9
Intensity Change (Log2) – – – 0.01 -0.15 -0.24 -0.44 -0.31 – -0.31 -0.28 -0.29 – 0.27 0.36 1.06 1.05 1.13 0.52 0.6 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Cytoplasm -4.5 -7.6 -7.3 -10.6 -11.5 – -11.8 -11.1 -12.5 – -1.2 -1.9 -1.5 -1.5 -0.7
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 3.2 2.9 – 2.7 4.5 5.0 – 0 0 0 0 0
Nucleus 1.5 4.8 4.8 4.2 4.9 – 5.3 5.5 5.0 – 0 0 0.2 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 2.6 2.8 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Vacuole 2.2 0.9 -2.0 -0.5 -0.6 – -1.2 -3.5 -2.5 – 0.5 0.9 -0.1 0.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.9434 24.5445 22.1556 19.6383 20.8799 22.5112 21.9992 23.6308 20.9027 17.4819 20.8672 22.4751 18.5156 20.7901 18.3574 -0.2884 17.8884 19.1841
Actin 0.0046 0.0043 0.0007 0.0001 0.0004 0.0002 0.0091 0 0.004 0.0049 0.0023 0.0001 0.0045 0.0001 0.0225 0.0018 0.0001 0.0019
Bud 0.0001 0.0001 0.0001 0 0.0008 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0001 0.0002 0 0.0004 0.0019 0.0001 0.0004
Bud Neck 0.0004 0.0001 0.0002 0.0001 0.0003 0.0005 0.0005 0.0001 0.0001 0.0005 0.0005 0.0004 0.0005 0.0001 0.0002 0.002 0.0002 0.0005
Bud Periphery 0.0001 0.0001 0.0002 0 0.001 0 0.0002 0 0.0001 0.0004 0 0 0.0002 0 0.0003 0.0021 0 0.0003
Bud Site 0.0012 0.0002 0.0003 0 0.0007 0.0001 0.0005 0 0.0002 0.0008 0.0001 0 0.0076 0 0.0005 0.0024 0.0001 0.0002
Cell Periphery 0.0003 0 0.0001 0 0.0002 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0064 0 0
Cytoplasm 0.0471 0.0507 0.0904 0.0533 0.0582 0.0822 0.0402 0.0999 0.092 0.0541 0.1757 0.0794 0.0446 0.0815 0.1063 0.0387 0.0561 0.2208
Cytoplasmic Foci 0.0085 0.0002 0.0039 0 0.0004 0.0171 0.0028 0.0001 0.0088 0.0333 0.0045 0.0001 0.0094 0 0.0057 0.0282 0 0.0041
Eisosomes 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0026 0.0004 0.0002 0.0001 0.001 0.0007 0.0023 0.0002 0.0008 0.0098 0.0013 0.0004 0.0006 0.0002 0.0004 0.0032 0.0002 0.0015
Endosome 0.0054 0.0006 0.0038 0 0.0009 0.0115 0.006 0.0001 0.0042 0.0461 0.0082 0.0002 0.0042 0.0001 0.0122 0.0206 0.0001 0.0087
Golgi 0.0016 0.001 0.0054 0 0.0001 0.0031 0.0013 0 0.0016 0.0058 0.0005 0 0.0024 0 0.0251 0.0145 0 0.0078
Lipid Particles 0.013 0.0001 0.0051 0 0.0001 0.0149 0.0014 0 0.003 0.0178 0.001 0 0.0041 0 0.0009 0.4388 0 0.0278
Mitochondria 0.0012 0.006 0.0098 0.0002 0.0082 0.0016 0.0025 0.0001 0.0034 0.0044 0.0005 0.0003 0.0109 0.0003 0.017 0.01 0.0006 0.0083
None 0.0012 0.0001 0.0001 0 0.0004 0.0001 0.0006 0 0.0015 0.0034 0.0065 0.0001 0.0017 0 0.0001 0.0073 0 0.0003
Nuclear Periphery 0.0372 0.0208 0.021 0.0184 0.0344 0.0349 0.0351 0.0226 0.0247 0.0355 0.0468 0.0286 0.035 0.0389 0.0222 0.0142 0.0313 0.0349
Nucleolus 0.0007 0.0006 0.0006 0.0006 0.004 0.0009 0.0009 0.0005 0.0004 0.0008 0.0008 0.0006 0.001 0.0005 0.0003 0.0841 0.0013 0.0003
Nucleus 0.8598 0.9127 0.8546 0.926 0.883 0.827 0.8911 0.875 0.8492 0.7658 0.7189 0.8877 0.8537 0.8773 0.7819 0.1065 0.9084 0.6601
Peroxisomes 0.0032 0.0001 0.0009 0 0.0002 0.0011 0.0017 0 0.0027 0.0019 0.0008 0 0.0023 0 0.0024 0.0168 0 0.007
Punctate Nuclear 0.0082 0.0009 0.0007 0.0008 0.0016 0.0013 0.0016 0.0009 0.0019 0.0094 0.0301 0.001 0.016 0.0005 0.0008 0.0071 0.0008 0.001
Vacuole 0.0025 0.0007 0.0013 0.0001 0.0028 0.0018 0.0012 0.0002 0.0008 0.003 0.0008 0.0007 0.0006 0.0003 0.0004 0.1455 0.0004 0.008
Vacuole Periphery 0.001 0.0003 0.0006 0.0001 0.0014 0.001 0.0007 0.0001 0.0004 0.0015 0.0005 0.0004 0.0004 0.0002 0.0005 0.0478 0.0003 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.1125 27.7295 27.1903 48.1224 27.2908 19.93 30.7909 41.9014 41.0986 40.5219
Translational Efficiency 0.7082 0.984 0.979 0.7185 0.9964 1.3435 0.8741 0.729 0.8556 0.7289

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; regulated by phosphorylation and ubiquitination; likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity; necessary for oxidant and ramped heat stress-induced cell death; ortholog of mammalian RAC1
Localization
Cell Percentages cytoplasm (26%), nucleus (3%), mixed (71%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rho5

Rho5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rho5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available