Standard name
Human Ortholog
Description Putative oxidoreductase; required for cell viability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0.23 0.24 0.2 0.18 0.16 0.12 0.1 0.08 0.09 0 0 0.07 0 0.13 0.13 0.18 0.06 0.06 0.09 0 0.06 0
Bud 0 0 0.06 0.06 0.1 0.07 0.12 0.08 0.13 0.1 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.08 0.13 0.22 0.11 0.09 0.06 0.08 0 0.05 0.06 0.09 0.07 0.17 0.12 0.12 0.23 0.11 0 0 0 0 0 0
Cytoplasm 0.27 0.46 0.3 0.27 0.37 0.2 0.24 0.25 0.26 0.32 0.24 0.3 0.34 0.38 0.37 0.6 0.65 0.47 0 0.09 0 0.15 0.12 0.16
Endoplasmic Reticulum 0.62 0.41 0.48 0.36 0.21 0.41 0.33 0.17 0.3 0.11 0.15 0.07 0.57 0.47 0.55 0 0 0 0.76 0.68 0.76 0.57 0.59 0.61
Endosome 0 0 0.05 0 0.15 0.07 0.07 0.21 0 0.08 0 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria 0.13 0.05 0 0.17 0.16 0.24 0.33 0.27 0.34 0.45 0.53 0.48 0 0 0 0 0 0 0 0 0 0.08 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0.11 0.05 0.13 0.05 0.18 0.06 0.05 0 0.05 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 38 35 36 43 49 29 22 20 21 5 5 17 8 18 13 14 5 4 18 5 12 8
Bud 1 1 10 9 17 16 37 21 28 25 20 28 0 0 0 2 2 2 0 0 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 4 3 3
Bud Site 0 0 0 0 0 0 0 4 1 4 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 22 32 20 22 19 21 9 14 14 21 9 40 34 17 23 9 0 0 6 3 5 6
Cytoplasm 12 18 50 40 65 49 75 64 58 82 56 68 46 89 102 84 65 37 3 6 3 20 27 34
Endoplasmic Reticulum 28 16 80 53 37 100 104 42 67 29 35 16 78 111 151 6 3 2 65 51 155 79 135 135
Endosome 0 0 8 5 26 18 21 52 7 21 8 18 1 0 0 1 0 5 1 0 0 2 2 0
Golgi 1 0 0 1 1 0 0 0 0 0 0 0 0 0 1 2 1 5 1 1 3 1 1 1
Mitochondria 6 2 6 25 29 59 104 69 75 115 122 109 4 1 1 5 1 2 1 1 6 11 13 8
Nucleus 0 0 0 0 1 1 0 1 0 0 2 0 3 0 2 2 0 1 0 0 0 0 2 0
Nuclear Periphery 1 0 0 0 0 0 1 0 1 0 3 0 0 0 0 0 0 0 0 1 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 6 11 12 8 10 8 3 4 5 3 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 2 1 4 8 3 2 1 2 2 2 2 0 1 3 0 0 0 0 0 0
Vac/Vac Membrane 1 0 6 1 2 4 7 7 10 13 10 14 0 27 13 18 5 14 5 3 2 7 16 16
Unique Cell Count 45 39 168 147 177 241 316 251 222 255 230 229 137 237 275 140 100 79 86 75 206 140 228 223
Labelled Cell Count 53 44 226 213 248 321 431 327 285 329 298 287 149 287 314 155 114 94 86 75 206 140 228 223


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.9 7.4 7.2 7.5 6.8 6.4 6.4 5.9 5.7 5.2 5.3 8.3 8.7 8.4 13.1 13.1 12.9 8.7 9.2 9.6
Std Deviation (1e-4) 0.8 1.2 1.1 1.8 2.0 1.2 1.3 1.6 1.3 1.2 1.0 1.0 1.4 1.1 1.3 2.7 3.0 2.5 1.3 1.6 1.8
Intensity Change (Log2) – – – -0.03 0.03 -0.13 -0.21 -0.21 -0.31 -0.37 -0.49 -0.47 0.18 0.24 0.18 0.83 0.83 0.81 0.24 0.32 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -0.5 -1.2 -1.9 -3.0 -3.4 -4.3 -3.7 -6.5 -4.7 -4.5 -6.6 -2.2 -1.9 -0.9
Bud 0.1 1.3 0.3 2.0 0.9 2.2 1.4 1.0 2.1 0 0 0 -2.0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.0 -0.5 -1.3 -2.7 -1.6 -3.3 -2.7 -2.4 -1.2 -1.9 1.0 -0.2 -0.3 2.1 -0.4
Cytoplasm -0.5 1.4 -2.2 -1.4 -1.0 -0.8 0.5 -1.2 0 0.7 1.6 1.6 5.3 5.6 2.6
Endoplasmic Reticulum -2.1 -5.2 -1.2 -3.2 -6.8 -3.5 -8.3 -7.0 -9.3 1.6 -0.2 1.5 -8.4 -7.6 -7.0
Endosome -0.6 3.1 1.1 0.8 4.6 -0.8 1.4 -0.6 1.2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 3.9 5.7 7.3 6.3 7.3 9.2 10.4 9.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.5 1.3 -0.1 -0.2 -0.2 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 -0.5 0.5 0.7 0.4 1.1 0 2.8 0.6 3.0 0 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7529 4.0374 3.0047 2.8927 2.5451 3.1518 3.399 4.4304 3.8409 3.9088 3.6707 3.9343 3.3693 4.4511 3.8129 3.4118 3.1691 3.943
Actin 0.0243 0.0081 0.0311 0.1054 0.0452 0.0086 0.0438 0.0138 0.0438 0.0304 0.0047 0.0167 0.0135 0.0033 0.0199 0.0118 0.0112 0.0064
Bud 0.0006 0.0012 0.0009 0.0005 0.0013 0.0005 0.0012 0.0004 0.0007 0.0007 0.0001 0.0002 0.002 0.0003 0.0012 0.0001 0.0026 0.0003
Bud Neck 0.0017 0.0026 0.0054 0.0019 0.0173 0.0085 0.0109 0.0028 0.0042 0.0216 0.0016 0.0139 0.0017 0.0036 0.0085 0.0022 0.0052 0.0088
Bud Periphery 0.0017 0.0031 0.0034 0.0022 0.0042 0.0018 0.0023 0.0007 0.0034 0.0062 0.0003 0.0006 0.0014 0.0006 0.0013 0.0004 0.0016 0.0005
Bud Site 0.0056 0.0686 0.0136 0.0023 0.0045 0.0007 0.0101 0.0284 0.0044 0.0025 0.0003 0.0003 0.003 0.0271 0.0257 0.0012 0.0016 0.0004
Cell Periphery 0.0161 0.0251 0.0373 0.0082 0.0119 0.0093 0.0051 0.0246 0.0213 0.009 0.009 0.0027 0.0074 0.0209 0.0202 0.0058 0.0048 0.0043
Cytoplasm 0.2485 0.2962 0.3033 0.1458 0.2154 0.2555 0.1889 0.3569 0.3065 0.2825 0.2416 0.3542 0.1891 0.2306 0.2365 0.1665 0.2149 0.2514
Cytoplasmic Foci 0.026 0.0157 0.0219 0.0244 0.019 0.0198 0.0387 0.0133 0.0161 0.0208 0.012 0.0145 0.0208 0.0162 0.0227 0.0178 0.0248 0.0148
Eisosomes 0.0015 0.0013 0.0018 0.0022 0.0007 0.0005 0.0008 0.0004 0.0005 0.0001 0.0003 0.0002 0.0006 0.0004 0.0007 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.5305 0.4367 0.4109 0.6037 0.4074 0.5558 0.4744 0.4689 0.486 0.5061 0.5435 0.508 0.6219 0.5704 0.5562 0.7043 0.5625 0.6318
Endosome 0.0297 0.019 0.0397 0.0295 0.0676 0.0495 0.0577 0.0225 0.025 0.036 0.0304 0.027 0.033 0.0345 0.0223 0.0166 0.0331 0.0227
Golgi 0.0174 0.0175 0.0224 0.0242 0.0278 0.0165 0.0223 0.011 0.0223 0.0167 0.0186 0.015 0.0118 0.0098 0.0136 0.0306 0.0156 0.0087
Lipid Particles 0.0437 0.0289 0.0353 0.0251 0.017 0.0233 0.0465 0.0339 0.0271 0.0128 0.067 0.0143 0.0358 0.0551 0.0365 0.0255 0.0566 0.0219
Mitochondria 0.0076 0.0085 0.0137 0.0029 0.0538 0.0061 0.0288 0.0016 0.0062 0.0018 0.0514 0.0061 0.003 0.0011 0.0079 0.0012 0.0175 0.0013
None 0.0248 0.0508 0.03 0.0041 0.0144 0.0266 0.0057 0.004 0.0054 0.0031 0.0049 0.006 0.0282 0.0061 0.0059 0.002 0.0077 0.0137
Nuclear Periphery 0.0062 0.0031 0.0065 0.0054 0.0417 0.0048 0.0195 0.0047 0.0075 0.0089 0.0047 0.0087 0.0102 0.0054 0.0059 0.0053 0.0055 0.0042
Nucleolus 0.0002 0.0007 0.0007 0.0001 0.0014 0.0001 0.0011 0.0002 0.0002 0.0015 0.0001 0.0002 0.0008 0.0003 0.0008 0 0.0025 0.0001
Nucleus 0.0012 0.0013 0.0024 0.0009 0.0068 0.0013 0.0042 0.0013 0.0049 0.0078 0.0012 0.0018 0.0038 0.0013 0.0027 0.0008 0.0082 0.0012
Peroxisomes 0.0033 0.0004 0.0022 0.0007 0.0034 0.0007 0.0191 0.0003 0.0058 0.0054 0.0016 0.0008 0.001 0.0003 0.0033 0.0008 0.006 0.0007
Punctate Nuclear 0.0022 0.0006 0.0043 0.0005 0.0237 0.0006 0.0041 0.0006 0.0004 0.0095 0.0013 0.0028 0.0042 0.0011 0.0024 0.0007 0.0126 0.0013
Vacuole 0.0065 0.0098 0.0125 0.009 0.0122 0.0083 0.011 0.0092 0.0068 0.0157 0.0043 0.005 0.0058 0.0111 0.0052 0.0059 0.0047 0.0049
Vacuole Periphery 0.0007 0.0006 0.0009 0.001 0.0031 0.0011 0.0038 0.0005 0.0015 0.0007 0.0011 0.0009 0.0009 0.0007 0.0006 0.0004 0.0006 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.4112 24.1759 21.5275 25.0258 35.6484 44.1478 23.8331 24.4723 24.831 33.5488
Translational Efficiency 1.6825 1.3846 1.3854 1.462 1.3811 1.4149 1.5579 1.2782 1.3686 1.2667

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative oxidoreductase; required for cell viability
Localization
Cell Percentages ER (96%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Pbr1

Pbr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pbr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available