Standard name
Human Ortholog
Description Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis by phosphorylating Ldb19p; positively regulates activity of the three Mep ammonium transport proteins; mediates inhibitory phosphorylation of Mep2p and Par32p; TOR complex negatively regulates Npr1p activity; NPR1 has a paralog, PRR2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.07 0.28 0.21 0.23 0.19 0.23 0.18 0.21 0.23 0 0 0 0.07 0.08 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.99 0.98 0.95 0.92 0.88 0.78 0.87 0.81 0.76 0.98 0.99 0.99 0.94 0.96 0.97 0.93 0.96 0.95 0.93 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.12 0 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 7 5 4 5 8 0 0 0 0 0 1 0 0 1 1 1 1
Bud 0 1 0 2 2 4 4 5 8 1 8 1 4 1 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 4 24 77 80 70 107 74 62 45 79 6 27 8 31 17 7 0 0 0 0 1 2
Cytoplasm 42 263 337 266 372 278 487 251 307 178 264 541 820 824 410 213 185 58 253 326 400 582 744
Endoplasmic Reticulum 0 0 5 6 16 11 47 38 13 16 37 11 7 10 10 9 3 0 1 3 16 30 18
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 2 1
Mitochondria 0 1 0 0 0 0 13 17 14 28 16 1 1 2 6 1 1 0 1 1 0 2 1
Nucleus 1 0 2 3 0 2 3 0 0 1 0 1 0 2 5 7 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 0 0 0 1 1 1 11 0 3 5 14 5 4 1 4 3 6 6 6
Unique Cell Count 43 264 341 272 390 301 552 321 352 219 347 551 830 836 435 223 191 63 264 343 431 642 791
Labelled Cell Count 43 269 370 354 470 366 668 391 409 275 423 561 863 853 478 254 204 63 264 343 431 642 791


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 13.4 13.5 12.5 13.6 11.3 10.9 9.7 9.0 8.6 9.7 13.0 13.1 12.6 16.5 17.3 17.2 11.9 12.7 13.5
Std Deviation (1e-4) 3.4 2.2 1.8 1.6 1.7 1.8 1.6 1.7 1.6 1.6 1.5 1.8 2.1 2.3 3.8 4.0 3.6 1.6 1.7 2.0
Intensity Change (Log2) -0.11 0.01 -0.25 -0.31 -0.47 -0.58 -0.65 -0.47 -0.05 -0.05 -0.1 0.29 0.36 0.35 -0.18 -0.08 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 7.1 5.2 5.8 5.1 5.8 4.2 4.7 5.8 -4.8 -2.9 -5.8 0 0.3 -1.6
Cytoplasm -1.0 -2.7 -4.1 -5.8 -8.4 -6.0 -7.5 -9.0 -0.7 0 -0.4 -3.3 -2.5 -1.6
Endoplasmic Reticulum 0 2.1 1.8 4.4 5.4 1.8 3.5 5.0 0.6 0 0 0.8 1.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 4.3 3.7 6.8 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 2.5 0 0 0 2.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5059 9.1961 8.5494 9.3463 8.2086 8.4733 6.003 8.5533 6.8453 6.6417 6.3201 7.4472 6.3073 9.7336 7.9364 7.2607 6.4825 8.4281
Actin 0.0263 0.0105 0.0035 0.001 0.0214 0.0021 0.0152 0.0001 0.0095 0.0001 0.0296 0.0048 0.0258 0.0001 0.0018 0.001 0.0045 0.0002
Bud 0.0008 0.0002 0.0002 0.0003 0.0392 0.0002 0.0008 0.0002 0.0009 0.0003 0.0021 0.0002 0.0005 0.0001 0.0003 0.0022 0.0005 0
Bud Neck 0.0098 0.0003 0.0004 0.0003 0.0018 0.001 0.0035 0.0002 0.0011 0.0002 0.0013 0.0006 0.001 0.0001 0.0002 0.001 0.001 0.0005
Bud Periphery 0.0008 0.0002 0.0001 0.0001 0.0113 0.0002 0.0008 0 0.0006 0 0.0058 0.0003 0.0007 0 0.0001 0.0026 0.0004 0
Bud Site 0.0023 0.0031 0.0005 0.0004 0.0019 0.002 0.0038 0.0015 0.0022 0.0001 0.0031 0.0002 0.0024 0.0001 0.0003 0.0003 0.0011 0
Cell Periphery 0.0008 0.001 0.0003 0.0001 0.0009 0.0001 0.0003 0.0001 0.0001 0 0.0004 0 0.0004 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.7852 0.9616 0.9287 0.9758 0.809 0.9366 0.7925 0.9874 0.8894 0.9382 0.8487 0.953 0.8268 0.9891 0.9507 0.9493 0.894 0.9764
Cytoplasmic Foci 0.0311 0.0051 0.0069 0.0065 0.0234 0.0046 0.0275 0.0031 0.0221 0.0066 0.0074 0.0051 0.0208 0.0011 0.0061 0.0094 0.0196 0.003
Eisosomes 0.0003 0.0001 0 0 0.0001 0 0.0002 0 0.0001 0 0.0003 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0407 0.0031 0.0092 0.0027 0.0042 0.017 0.0121 0.0012 0.0038 0.0109 0.0046 0.0044 0.0197 0.0066 0.0075 0.0028 0.0022 0.0069
Endosome 0.0235 0.0035 0.0031 0.0056 0.0285 0.0088 0.0644 0.0034 0.0283 0.0231 0.0189 0.0105 0.0296 0.0005 0.0144 0.0144 0.0337 0.0031
Golgi 0.0068 0.0006 0.0013 0.0004 0.022 0.0021 0.0223 0.0002 0.0114 0.0007 0.0223 0.0055 0.0078 0.0001 0.0017 0.0014 0.0093 0.0004
Lipid Particles 0.0083 0.0014 0.0033 0.0001 0.0006 0.0017 0.0082 0 0.0028 0.0003 0.0046 0.0017 0.0167 0 0.0011 0.0002 0.0047 0.0054
Mitochondria 0.0023 0.0009 0.0011 0.0001 0.0051 0.0012 0.0208 0 0.0103 0.0002 0.0353 0.004 0.0039 0 0.0013 0.0042 0.0033 0.0011
None 0.0176 0.0038 0.0016 0.002 0.0245 0.0111 0.0044 0.0011 0.0019 0.0018 0.0037 0.0009 0.012 0.0011 0.0019 0.0016 0.0026 0.0005
Nuclear Periphery 0.0154 0.0003 0.0031 0.0006 0.0007 0.0021 0.0034 0.0001 0.0011 0.002 0.0011 0.0042 0.0078 0.0002 0.0062 0.0007 0.0031 0.0004
Nucleolus 0.0006 0.0001 0.0001 0 0.0002 0.001 0.0002 0 0.0002 0 0.0007 0.0001 0.0008 0 0.0002 0.0002 0.0005 0
Nucleus 0.0042 0.0005 0.0134 0.0009 0.0011 0.0024 0.0031 0.0005 0.0032 0.0012 0.0014 0.0009 0.0031 0.0004 0.0022 0.0017 0.0017 0.0005
Peroxisomes 0.0123 0.0013 0.0217 0.0001 0.0011 0.0004 0.0044 0 0.0044 0 0.0026 0.0016 0.0092 0 0.0002 0.0006 0.0032 0.0007
Punctate Nuclear 0.0062 0.0002 0.0003 0.0002 0.0002 0.004 0.0021 0 0.002 0.0001 0.0004 0.0005 0.006 0 0.0003 0.0002 0.0105 0.0001
Vacuole 0.0037 0.002 0.001 0.0026 0.0023 0.0011 0.0073 0.0009 0.0038 0.0126 0.0037 0.0009 0.0036 0.0003 0.0024 0.0049 0.0032 0.0005
Vacuole Periphery 0.0011 0.0001 0.0002 0.0003 0.0006 0.0003 0.0026 0.0001 0.001 0.0016 0.0017 0.0005 0.0011 0 0.0011 0.0013 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4845 10.8073 14.4968 16.9396 11.756 13.477 15.5504 20.1399 16.2135 19.9744
Translational Efficiency 0.9707 1.3471 1.0548 0.8951 1.2583 1.4506 1.3694 1.0704 1.2808 1.0473

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis by phosphorylating Ldb19p; positively regulates activity of the three Mep ammonium transport proteins; mediates inhibitory phosphorylation of Mep2p and Par32p; TOR complex negatively regulates Npr1p activity; NPR1 has a paralog, PRR2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Npr1

Npr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available