Standard name | |
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Human Ortholog | |
Description | Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 5 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 9 | 6 | 3 | 12 | 5 | 11 | – | 4 | 6 | 11 | 8 | 16 | 24 | 35 | 20 | 14 | 10 | 0 | 2 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 15 | 4 | 5 | 0 | 0 | 1 | 1 | 4 | 0 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 3 | 1 | 1 | 1 | 0 | 1 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 3 |
Mitochondria | 5 | 19 | 1 | 7 | 3 | 23 | 34 | – | 53 | 48 | 69 | 50 | 16 | 1 | 9 | 6 | 10 | 7 | 0 | 3 | 1 | 0 | 2 | 2 |
Nucleus | 132 | 235 | 198 | 171 | 273 | 229 | 454 | – | 186 | 241 | 193 | 323 | 253 | 292 | 284 | 362 | 248 | 202 | 143 | 239 | 193 | 176 | 228 | 186 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 1 | 0 | 1 | – | 0 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 2 | 0 | 1 | 0 | 2 | 13 | – | 14 | 31 | 33 | 29 | 2 | 7 | 6 | 2 | 1 | 1 | 0 | 0 | 0 | 3 | 15 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 4 | 7 | 3 | 0 | 3 | 2 | 5 | – | 2 | 2 | 2 | 3 | 0 | 5 | 6 | 27 | 16 | 8 | 3 | 4 | 7 | 10 | 41 | 47 |
Unique Cell Count | 144 | 255 | 208 | 174 | 288 | 242 | 488 | 203 | 280 | 232 | 361 | 271 | 328 | 329 | 408 | 278 | 222 | 153 | 259 | 208 | 200 | 309 | 275 | |
Labelled Cell Count | 150 | 272 | 210 | 182 | 294 | 261 | 522 | 265 | 335 | 310 | 418 | 288 | 332 | 343 | 438 | 298 | 236 | 153 | 259 | 208 | 200 | 309 | 275 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 6.9 | 7.1 | 6.6 | 7.4 | 5.9 | 6.2 | – | 5.5 | 5.5 | 5.1 | 6.2 | 7.8 | 8.3 | 8.0 | 8.4 | 8.4 | 8.0 | 7.8 | 8.1 | 8.4 |
Std Deviation (1e-4) | 2.0 | 1.4 | 1.3 | 1.1 | 1.5 | 1.4 | 1.4 | – | 1.2 | 1.3 | 1.2 | 1.4 | 2.1 | 2.3 | 2.2 | 1.9 | 2.0 | 1.8 | 2.1 | 2.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.07 | -0.28 | -0.21 | – | -0.38 | -0.36 | -0.47 | -0.21 | 0.13 | 0.22 | 0.17 | 0.24 | 0.24 | 0.17 | 0.14 | 0.19 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.8 | -0.6 | -0.5 | – | 0 | -0.5 | 1.0 | -0.5 | 1.6 | 2.2 | 3.3 | 1.2 | 1.2 | 0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.2 | 3.6 | – | 7.7 | 6.1 | 8.4 | 5.4 | 3.2 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.7 | -0.2 | -0.3 | -1.1 | – | -1.5 | -3.3 | -4.0 | -2.4 | -0.8 | -2.5 | -3.3 | -2.6 | -2.4 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 3.9 | 5.0 | 5.7 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.4 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.597 | 5.7924 | 5.3358 | 4.7374 | 4.3003 | 5.0457 | 4.1564 | 4.3441 | 4.0499 | 3.4685 | 3.3449 | 3.6194 | 5.3163 | 9.3354 | 4.3262 | 3.5785 | 4.0343 | 9.4695 |
Actin | 0.0144 | 0.0004 | 0.0058 | 0.037 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.003 | 0 | 0.0041 | 0.0068 | 0.0008 | 0.0003 |
Bud | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0069 | 0.0002 | 0 |
Bud Neck | 0.0017 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0004 | 0.0008 | 0.0011 | 0 | 0.0001 | 0.0003 | 0.0007 | 0.0005 |
Bud Periphery | 0.0012 | 0 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0008 | 0 | 0.0003 | 0.0031 | 0.0005 | 0 |
Bud Site | 0.0053 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.001 | 0.0005 | 0.0017 | 0 | 0.001 | 0.0015 | 0.0002 | 0 |
Cell Periphery | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0007 | 0.0001 | 0 |
Cytoplasm | 0.0015 | 0.0001 | 0.0008 | 0.0001 | 0.0027 | 0 | 0.0011 | 0.0001 | 0.001 | 0 | 0.0006 | 0.0013 | 0.0067 | 0.0188 | 0.0144 | 0.0393 | 0.0003 | 0.0114 |
Cytoplasmic Foci | 0.0093 | 0.0018 | 0.0019 | 0.0019 | 0.0022 | 0 | 0.0023 | 0 | 0.0002 | 0 | 0.0056 | 0.0006 | 0.0117 | 0.0001 | 0.0177 | 0.0216 | 0 | 0 |
Eisosomes | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0015 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0009 | 0.0003 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0029 | 0.0009 | 0.0018 | 0.0064 | 0.0004 | 0.0003 |
Endosome | 0.0049 | 0.0002 | 0.002 | 0.0006 | 0.0028 | 0 | 0.0065 | 0.0002 | 0.0015 | 0 | 0.004 | 0.0015 | 0.0061 | 0.0003 | 0.0124 | 0.0441 | 0.0004 | 0.0002 |
Golgi | 0.0024 | 0.0004 | 0.0007 | 0.0026 | 0.0002 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0067 | 0.0032 | 0 | 0 |
Lipid Particles | 0.0091 | 0.0038 | 0.0029 | 0.004 | 0.0017 | 0 | 0.0129 | 0 | 0.0001 | 0 | 0.0083 | 0.0001 | 0.0038 | 0.0001 | 0.0054 | 0.0023 | 0 | 0 |
Mitochondria | 0.0038 | 0.0002 | 0.0111 | 0.0007 | 0.0003 | 0.0001 | 0.0038 | 0.0009 | 0.0016 | 0 | 0.0015 | 0.0001 | 0.0023 | 0.0001 | 0.0053 | 0.0384 | 0.0022 | 0.0001 |
None | 0.0039 | 0 | 0.0006 | 0.0001 | 0.0041 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0029 | 0 | 0.0034 | 0.0081 | 0.0001 | 0 |
Nuclear Periphery | 0.0073 | 0.001 | 0.0024 | 0.0008 | 0.0063 | 0.0002 | 0.0047 | 0.0058 | 0.0048 | 0.0001 | 0.0022 | 0.0002 | 0.039 | 0.0797 | 0.0012 | 0.0153 | 0.0158 | 0.1306 |
Nucleolus | 0.0186 | 0.0049 | 0.0038 | 0.0028 | 0.0145 | 0.0236 | 0.007 | 0.0061 | 0.0039 | 0.0034 | 0.0354 | 0.0181 | 0.0156 | 0.0035 | 0.0033 | 0.0303 | 0.0239 | 0.0121 |
Nucleus | 0.886 | 0.9769 | 0.9631 | 0.9374 | 0.9292 | 0.9756 | 0.9465 | 0.9846 | 0.9824 | 0.9964 | 0.9306 | 0.9729 | 0.8854 | 0.8943 | 0.9154 | 0.751 | 0.9388 | 0.8419 |
Peroxisomes | 0.0056 | 0.0098 | 0.0024 | 0.0103 | 0.0001 | 0 | 0.0016 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0003 | 0 | 0.0048 | 0.0032 | 0 | 0 |
Punctate Nuclear | 0.0164 | 0.0003 | 0.001 | 0.0005 | 0.0334 | 0.0001 | 0.0032 | 0.0005 | 0.0006 | 0 | 0.0035 | 0.0002 | 0.0126 | 0.0014 | 0.001 | 0.0089 | 0.0139 | 0.0011 |
Vacuole | 0.0046 | 0.0001 | 0.0003 | 0 | 0.0007 | 0.0001 | 0.0045 | 0.0003 | 0.0013 | 0 | 0.0035 | 0.003 | 0.0016 | 0.0002 | 0.0007 | 0.0043 | 0.0006 | 0.0002 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0016 | 0.0006 | 0.0006 | 0 | 0.0008 | 0.0001 | 0.0013 | 0.0007 | 0.0005 | 0.0042 | 0.0009 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.6904 | 11.4828 | 15.3374 | 21.9843 | 22.4834 | 14.9778 | 13.5453 | 15.8029 | 15.5436 | 27.6353 |
Translational Efficiency | 0.6953 | 0.8305 | 0.6731 | 0.4576 | 0.6084 | 0.9048 | 0.7928 | 0.426 | 0.5627 | 0.4937 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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