Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth; contains two PH-like domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.12 0.09 0.08 0.06 0.07 0.09 0.08 0.08 0.07 0.05 0.07 0.11 0.1 0.11 0.12 0.05 0.08 0.09 0.07 0.13 0.1 0.13 0.14
Bud 0 0 0.06 0.07 0.05 0.07 0.1 0.13 0.15 0.12 0.14 0.12 0 0 0 0.05 0 0 0 0 0.07 0.05 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Cytoplasm 0.62 0.77 0.57 0.58 0.53 0.42 0.33 0.24 0.28 0.23 0.29 0.26 0.73 0.72 0.67 0.65 0.71 0.68 0.48 0.65 0.37 0.4 0.43 0.46
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.11 0.06 0.06 0.06
Endosome 0.13 0.09 0.18 0.17 0.23 0.25 0.27 0.22 0.18 0.16 0.1 0.16 0.11 0.1 0.12 0.05 0.07 0 0.07 0.05 0.06 0.09 0.08 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.09 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.13 0.16 0.11 0.14 0.16 0.2 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0 0.06 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.06 0.07 0.12 0.13 0.19 0.2 0.21 0.12 0.19 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.14 0 0.17 0.12 0.12 0.14 0.2 0.31 0.28 0.34 0.29 0.37 0.07 0.07 0.15 0.15 0.07 0.1 0.14 0.09 0.18 0.17 0.12 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 37 27 24 16 30 33 44 30 29 19 31 14 14 32 36 14 15 22 21 40 16 37 41
Bud 6 9 20 19 13 29 35 74 58 52 54 52 5 5 7 14 5 5 11 14 21 7 14 13
Bud Neck 0 0 0 0 0 0 1 0 2 4 2 2 0 0 0 0 0 0 11 8 6 3 8 8
Bud Site 0 1 1 0 1 2 5 6 18 24 15 16 0 0 0 1 0 0 – – – – – –
Cell Periphery 5 15 17 13 10 13 11 11 5 10 15 21 5 5 7 14 8 14 0 1 0 0 1 1
Cytoplasm 141 233 182 163 141 175 123 137 104 101 116 110 97 97 203 195 190 128 127 213 119 64 125 142
Endoplasmic Reticulum 2 14 3 9 6 10 9 12 9 9 12 7 5 5 9 8 14 7 11 14 34 9 16 18
Endosome 29 28 56 48 61 103 101 123 66 72 38 69 14 14 35 14 18 5 19 15 18 14 24 30
Golgi 10 1 6 3 5 2 4 3 0 0 0 2 0 0 6 14 22 16 4 1 4 1 6 6
Mitochondria 5 2 5 16 9 53 59 62 51 70 80 68 0 0 0 7 2 2 2 2 3 3 3 4
Nucleus 2 0 0 1 1 3 3 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 0 2 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 1 2 8 5 5 17 21 23 23 28 28 0 0 2 2 3 2 0 0 0 0 0 0
Peroxisomes 3 0 33 18 19 51 48 110 75 93 47 79 5 4 4 1 0 1 5 1 10 6 9 5
SpindlePole 31 5 55 33 32 57 72 178 105 149 118 158 9 9 46 45 20 19 37 28 57 27 34 24
Vac/Vac Membrane 2 1 2 3 6 11 5 15 10 9 9 8 0 0 1 9 8 13 8 1 0 1 2 4
Unique Cell Count 227 303 317 283 265 420 368 568 376 442 400 427 133 134 304 300 267 188 267 328 325 160 289 309
Labelled Cell Count 260 347 409 358 325 544 526 798 558 645 554 651 154 153 353 361 304 227 267 328 325 160 289 309


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 11.1 10.8 10.1 10.5 9.5 9.3 9.9 9.0 8.8 8.9 9.2 11.0 11.0 11.5 11.6 12.9 15.7 10.8 11.9 12.7
Std Deviation (1e-4) 1.6 1.4 2.1 1.6 2.0 1.7 1.7 2.1 1.8 1.9 2.1 2.3 1.4 1.4 1.4 2.1 3.4 3.2 1.8 2.2 1.9
Intensity Change (Log2) – – – -0.08 -0.03 -0.19 -0.22 -0.12 -0.25 -0.28 -0.28 -0.23 0.03 0.03 0.09 0.11 0.26 0.55 0.0 0.15 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.1 -0.7 0.2 -0.4 -0.3 -1.0 -2.0 -0.6 0.7 0.7 0.9 1.4 -1.5 -0.2
Bud 0.2 -0.7 0.3 1.5 3.1 3.8 2.5 3.1 2.7 -1.1 -1.1 -2.4 -0.9 -2.6 -1.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.6 3.9 3.1 3.1 0 0 0 0 0 0
Cell Periphery -0.4 -0.9 -1.5 -1.6 -2.8 -3.0 -2.3 -1.0 -0.3 -0.7 -0.7 -2.0 -0.4 -1.4 0.9
Cytoplasm 0 -1.0 -4.2 -6.3 -9.9 -7.9 -9.7 -7.7 -8.7 3.1 3.0 2.4 1.9 3.4 2.4
Endoplasmic Reticulum 2.0 0 1.5 1.5 1.3 1.5 1.2 1.9 0 0 0 1.8 1.6 3.1 0
Endosome -0.2 1.6 2.2 3.0 1.4 0 -0.5 -3.2 -0.5 -1.9 -1.9 -2.2 -5.1 -4.0 -5.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.1 1.9 3.6 3.5
Mitochondria 2.7 1.4 5.5 6.5 5.0 5.8 6.5 7.6 6.5 0 0 0 0.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.2 2.7 3.9 3.5 4.2 4.1 0 0 0 0 0 0
Peroxisomes -1.8 -1.4 0.7 1.1 3.5 3.4 3.9 0.6 3.1 -2.3 -2.6 -4.8 -5.5 -5.4 -4.3
SpindlePole -2.0 -1.8 -1.4 0.7 4.5 3.3 5.0 3.8 5.9 -2.9 -3.0 -0.7 -0.8 -3.5 -2.2
Vacuole 0 0 2.0 0 2.1 2.0 0 0 0 0 0 0 2.2 0 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1006 7.9131 7.3359 7.538 6.7547 7.0958 7.42 8.9053 7.9912 7.4782 7.0049 8.2896 8.6744 9.3821 9.361 10.2039 8.6975 9.3952
Actin 0.0255 0.0371 0.0179 0.0354 0.0151 0.0126 0.0798 0.0246 0.0303 0.0552 0.0408 0.0257 0.0198 0.0284 0.0352 0.0399 0.0604 0.0173
Bud 0.0008 0.0025 0.0029 0.0013 0.001 0.0003 0.0012 0.0024 0.0009 0.0006 0.0007 0.0015 0.0005 0.0013 0.0004 0.0002 0.0002 0.0003
Bud Neck 0.0006 0.0072 0.0104 0.0095 0.0016 0.0106 0.0124 0.0018 0.0188 0.0289 0.0224 0.0059 0.001 0.0017 0.0041 0.0041 0.0023 0.0038
Bud Periphery 0.0003 0.0012 0.0016 0.0008 0.0005 0.0001 0.0015 0.0023 0.0009 0.0004 0.0004 0.0015 0.0004 0.0004 0.0002 0.0001 0.0001 0.0002
Bud Site 0.0164 0.0437 0.0215 0.0042 0.0023 0.0009 0.0154 0.0528 0.0182 0.0016 0.0037 0.0024 0.0254 0.0232 0.012 0.0009 0.0007 0.0007
Cell Periphery 0.0005 0.0004 0.0003 0.0003 0.0001 0.0001 0.0017 0.0003 0.0019 0.0001 0.0002 0.0002 0.0008 0.0003 0.0003 0 0.0001 0.0001
Cytoplasm 0.3123 0.2879 0.2786 0.2938 0.1469 0.2791 0.2178 0.2053 0.2843 0.199 0.2972 0.2775 0.2217 0.1851 0.2422 0.0857 0.2162 0.2223
Cytoplasmic Foci 0.4737 0.4578 0.4812 0.4968 0.6841 0.5093 0.4315 0.5061 0.4267 0.4538 0.4492 0.4741 0.5388 0.4854 0.5228 0.6483 0.5432 0.4943
Eisosomes 0 0 0 0 0 0 0.0011 0.0001 0.0004 0 0.0002 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0179 0.003 0.0073 0.006 0.0011 0.0168 0.0114 0.0008 0.0159 0.0273 0.0019 0.0092 0.0103 0.0032 0.006 0.0249 0.0042 0.0166
Endosome 0.0423 0.0367 0.0728 0.0414 0.0407 0.052 0.0625 0.0206 0.0623 0.1141 0.05 0.0568 0.055 0.0591 0.0385 0.0542 0.0936 0.0381
Golgi 0.0107 0.0166 0.0154 0.0088 0.019 0.0158 0.0143 0.0082 0.0172 0.0183 0.0282 0.0206 0.0199 0.0156 0.0125 0.0147 0.014 0.0131
Lipid Particles 0.0294 0.0117 0.0161 0.0355 0.0161 0.0267 0.0306 0.0313 0.0098 0.0143 0.0048 0.0132 0.0244 0.0144 0.0166 0.0178 0.0214 0.0333
Mitochondria 0.0005 0.0008 0.0011 0.0007 0.0005 0.0006 0.0016 0.0005 0.0033 0.0006 0.0455 0.0064 0.0005 0.0005 0.0004 0.0004 0.0006 0.0007
None 0.0059 0.001 0.0023 0.0021 0.0004 0.0007 0.0411 0.0059 0.0032 0.0011 0.0006 0.0108 0.0033 0.0011 0.0012 0.0007 0.0024 0.0008
Nuclear Periphery 0.0049 0.0005 0.0038 0.0033 0.0002 0.0004 0.0034 0.0001 0.0022 0.0014 0.0002 0.0067 0.0008 0.0004 0.0005 0.0028 0.0002 0.0005
Nucleolus 0.0002 0.0033 0.0003 0.0001 0.0001 0.0001 0.0014 0.0002 0.0002 0 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0028 0.0019 0.0016 0.0019 0.0002 0.0004 0.0029 0.0003 0.0006 0.0005 0.0002 0.0015 0.0006 0.0005 0.0004 0.0005 0.0006 0.0005
Peroxisomes 0.0374 0.0625 0.0462 0.0494 0.0572 0.0589 0.0355 0.1242 0.0846 0.0744 0.0506 0.0532 0.0594 0.137 0.0767 0.0846 0.0339 0.1302
Punctate Nuclear 0.0148 0.0227 0.016 0.0074 0.0117 0.0134 0.0299 0.0116 0.0142 0.0052 0.0026 0.0307 0.0154 0.0408 0.0292 0.0192 0.0038 0.0258
Vacuole 0.0024 0.0014 0.0023 0.0011 0.0011 0.0007 0.0026 0.0006 0.0036 0.0026 0.0003 0.0012 0.0016 0.0012 0.0007 0.0008 0.0016 0.0011
Vacuole Periphery 0.0004 0.0001 0.0005 0.0003 0.0002 0.0002 0.0005 0.0001 0.0006 0.0004 0.0002 0.0005 0.0002 0.0002 0.0001 0.0002 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.0847 26.4545 18.384 24.5823 27.1018 28.6379 26.5359 32.0946 28.1213 28.0701
Translational Efficiency 1.6179 1.3395 1.7045 1.4127 1.1292 0.8951 1.309 0.8465 0.9967 1.0729

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth; contains two PH-like domains
Localization
Cell Percentages cytoplasm (63%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Vid27

Vid27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vid27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available