Standard name
Human Ortholog
Description Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.15 – 0.19 0.22 0.11 0.1 0.13 0.11 0.08 0.08 0.09 0.14 – 0.3 0.06 0.11 0.08 0.05 0.06 0.06 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 – 0.11 0.05 0.22 0.17 0.16 0.46 0.31 0.62 0.36 0 – 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0.6 0.6 – 0.64 0.59 0.69 0.72 0.73 0.65 0.72 0.6 0.7 0.69 – 0.66 0.8 0.78 0.78 0.61 0.62 0.43 0.79 0.79 0.73
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.38 – 0.11 0.17 0.08 0.1 0.05 0 0.11 0.06 0.08 0.09 – 0.06 0.15 0.09 0.13 0.24 0.23 0.3 0.13 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1
Bud 0 0 – 0 0 1 0 3 0 1 3 0 0 – 0 1 0 0 1 2 3 0 1 2
Bud Neck 0 0 – 0 0 2 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 – 0 0 0 1 2 2 1 3 1 0 – 0 1 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0
Cytoplasm 18 26 – 18 24 20 25 31 15 19 14 20 5 – 16 17 29 22 11 12 2 1 0 2
Endoplasmic Reticulum 0 1 – 0 1 0 0 0 0 0 1 1 0 – 0 5 5 11 0 0 1 0 0 0
Endosome 3 1 – 1 2 8 4 4 1 6 0 0 0 – 0 3 6 7 1 2 1 0 1 0
Golgi 0 1 – 0 1 0 0 0 0 0 1 0 0 – 0 0 1 3 1 2 0 0 0 0
Mitochondria 42 6 – 11 5 39 44 40 63 71 106 77 1 – 1 13 15 12 4 3 1 0 0 1
Nucleus 111 104 – 62 64 122 189 179 90 165 102 151 24 – 35 212 215 214 144 127 22 23 39 72
Nuclear Periphery 0 0 – 0 0 0 1 0 0 0 0 1 0 – 0 1 0 0 1 0 0 0 0 0
Nucleolus 1 0 – 0 0 1 1 0 1 1 5 1 2 – 0 1 4 3 1 0 0 0 0 1
Peroxisomes 0 0 – 0 0 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 2 2 1 1 3 1 1 0 3 0 – 1 1 1 2 9 4 0 0 1 1
Vac/Vac Membrane 42 65 – 11 18 15 25 13 5 24 11 17 3 – 3 39 25 37 55 47 15 4 3 10
Unique Cell Count 186 172 97 109 178 262 246 138 228 171 215 35 53 265 274 275 238 207 52 30 50 100
Labelled Cell Count 217 204 105 117 210 292 277 178 289 246 272 35 56 295 302 311 238 207 52 30 50 100


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.0 – 5.1 6.3 4.9 5.2 5.5 4.0 4.7 4.1 4.6 6.0 – 6.8 7.0 7.0 6.8 – – 5.5
Std Deviation (1e-4) 0.8 1.0 – 1.6 2.7 1.6 2.0 1.8 0.9 1.3 1.5 1.6 1.7 – 2.4 1.6 1.6 1.6 – – 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4518 0.7075 0.7711 0.5489 0.128 0.5083 0.2287 0.4881 0.5279 0.0781 0.8559 0.527 -0.3662 -0.2341 -0.597 0.0643 0.605 -0.5263
Actin 0.0048 0.0001 0.0024 0.0048 0.0013 0.0007 0.0385 0.0062 0.0035 0.0001 0.0262 0.0044 0.041 0.0002 0.0002 0.0172 0.0497 0.0045
Bud 0.0054 0.0113 0.0015 0.0002 0.0037 0.0011 0.0017 0.0001 0.0003 0 0.0006 0.0003 0.001 0.0001 0 0.0021 0.0011 0.0003
Bud Neck 0.0019 0.0006 0.0013 0.0006 0.0008 0.003 0.0026 0.0004 0.0013 0.0001 0.0009 0.0087 0.0045 0.0002 0.0004 0.0011 0.0017 0.0035
Bud Periphery 0.0012 0.0021 0.0011 0.0009 0.0147 0.0007 0.0032 0.0001 0.0008 0 0.0012 0.0006 0.002 0 0 0.0027 0.0018 0.0006
Bud Site 0.0063 0.0097 0.006 0.0002 0.0007 0.001 0.0235 0.0015 0.0032 0 0.0025 0.0004 0.0064 0.0003 0.0002 0.0131 0.0037 0.0002
Cell Periphery 0.0002 0.0002 0.0002 0.0004 0.0003 0.0001 0.0009 0 0.0004 0 0.0003 0.0001 0.0005 0 0 0.0003 0.0004 0.0001
Cytoplasm 0.0138 0.013 0.0076 0.0036 0.0014 0.0172 0.0327 0.0177 0.0114 0.017 0.0049 0.0042 0.0184 0.0263 0.0196 0.0022 0.0159 0.013
Cytoplasmic Foci 0.0091 0.0008 0.0126 0.0022 0.0021 0.0036 0.0105 0.0015 0.0075 0.0002 0.0083 0.0039 0.0186 0.0084 0.0007 0.0167 0.0175 0.0238
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0005 0.0001 0.0001 0 0.0011 0.0001 0.0003 0 0 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.0019 0.0012 0.0025 0.0015 0.0007 0.0007 0.0066 0.0004 0.0018 0.0003 0.0013 0.0002 0.0033 0.0013 0.0061 0.0002 0.0085 0.0012
Endosome 0.017 0.0012 0.0116 0.0086 0.0063 0.0034 0.0269 0.0004 0.0106 0.0002 0.0159 0.0015 0.034 0.0015 0.0047 0.0103 0.1588 0.0064
Golgi 0.0041 0 0.0051 0.0064 0.0044 0.0023 0.0079 0.0004 0.003 0 0.0131 0.0026 0.0095 0.0005 0.0001 0.0097 0.0564 0.0064
Lipid Particles 0.009 0.0001 0.0123 0.0085 0.0145 0.0012 0.0153 0.0011 0.0075 0 0.0073 0.0005 0.0146 0.0054 0.0004 0.0068 0.0343 0.0021
Mitochondria 0.014 0.0006 0.0206 0.0303 0.0042 0.008 0.0204 0.0053 0.021 0.0004 0.0435 0.0058 0.0092 0.0003 0.0004 0.0069 0.0574 0.0148
None 0.0262 0.0099 0.0129 0.0014 0.0005 0.0177 0.0303 0.0356 0.041 0.0107 0.0022 0.0287 0.0418 0.0425 0.0432 0.001 0.0451 0.0122
Nuclear Periphery 0.0241 0.0209 0.0172 0.0189 0.0059 0.009 0.0312 0.0074 0.0074 0.013 0.0081 0.0199 0.0118 0.0188 0.0114 0.0028 0.023 0.0065
Nucleolus 0.011 0.0088 0.0093 0.01 0.0196 0.0169 0.009 0.0058 0.006 0.0037 0.006 0.0082 0.0061 0.0066 0.0064 0.0034 0.0134 0.0094
Nucleus 0.8051 0.8842 0.8165 0.8105 0.9017 0.8714 0.699 0.906 0.8601 0.9481 0.8364 0.9003 0.7431 0.8638 0.896 0.8726 0.4622 0.8506
Peroxisomes 0.0073 0 0.0092 0.0017 0.0017 0.0009 0.0122 0.005 0.0045 0 0.0058 0.0018 0.0188 0.009 0 0.0223 0.0039 0.0004
Punctate Nuclear 0.0323 0.0344 0.0467 0.0849 0.0121 0.0396 0.0168 0.0045 0.0069 0.0057 0.0124 0.0074 0.007 0.0136 0.0089 0.005 0.0223 0.0418
Vacuole 0.0029 0.0007 0.002 0.0016 0.0025 0.0011 0.0069 0.0004 0.0012 0.0001 0.001 0.0003 0.0061 0.0008 0.0008 0.0029 0.0088 0.0012
Vacuole Periphery 0.0024 0.0002 0.0013 0.0025 0.001 0.0005 0.0033 0.0001 0.0006 0.0001 0.001 0.0003 0.002 0.0002 0.0003 0.0006 0.0138 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0123 9.5059 9.1284 9.4411 10.9486 10.6278 9.046 9.1582 8.2663 12.9363
Translational Efficiency 1.1264 0.648 0.6389 0.7762 1.1289 1.3984 0.7473 0.8286 0.9778 0.8155

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (33%), cytoplasm (3%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mgs1

Mgs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mgs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available