Standard name
Human Ortholog
Description Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.93 0.89 0.62 0.63 0.51 0.36 0.37 0.37 0.31 0.32 0.32 0.94 0.85 0.9 0.79 0.8 0.81 0.87 0.86 0.83 0.94 0.89 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0.12 0.08 0.26 0.36 0.27 0.46 0.55 0.5 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.05 0.18 0.19 0.24 0.18 0.23 0.21 0.24 0 0 0 0 0 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.06 0.08 0.33 0.35 0.29 0.29 0.28 0.22 0.18 0.23 0.18 0.17 0.23 0.17 0.31 0.27 0.16 0 0.05 0.05 0 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud 0 0 0 0 2 0 2 5 4 6 1 1 0 0 0 0 0 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 6
Bud Site 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 2 2 0 0 0 0 0 0 0
Cytoplasm 88 219 148 95 108 116 117 130 80 101 94 103 170 331 316 295 173 117 98 219 164 154 208 182
Endoplasmic Reticulum 1 3 2 0 0 1 0 1 4 1 1 0 1 0 2 9 9 5 0 0 3 0 1 1
Endosome 0 0 0 5 7 0 0 6 0 0 0 0 3 6 4 9 4 0 2 3 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Mitochondria 15 5 5 18 14 58 119 96 100 180 145 189 0 2 4 8 5 1 0 1 5 0 3 1
Nucleus 0 2 2 2 9 40 61 85 40 74 60 76 0 4 3 13 9 17 2 9 6 2 6 4
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 4
Vac/Vac Membrane 9 14 14 51 60 65 94 99 47 60 68 56 30 89 58 115 59 23 4 11 10 3 6 20
Unique Cell Count 102 235 167 154 171 226 328 353 217 327 290 317 180 389 350 374 215 144 114 256 198 164 235 229
Labelled Cell Count 114 243 172 171 200 280 395 422 278 422 371 427 204 433 388 452 262 163 114 256 198 164 235 229


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.1 5.1 4.9 5.3 4.9 4.4 5.1 4.7 4.5 4.5 4.3 6.1 6.0 6.0 7.0 8.1 8.6 5.2 5.6 6.3
Std Deviation (1e-4) 0.5 0.8 0.9 0.9 1.3 0.9 1.1 1.0 0.8 0.9 1.0 0.9 1.1 1.1 1.0 1.3 1.4 1.2 0.9 1.5 1.4
Intensity Change (Log2) -0.04 0.06 -0.04 -0.19 -0.0 -0.1 -0.17 -0.16 -0.23 0.27 0.24 0.25 0.47 0.68 0.76 0.04 0.15 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.6 -5.5 -7.8 -11.2 -11.1 -10.2 -12.1 -11.6 -11.7 2.0 -1.1 0.6 -2.7 -2.2 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 2.1 6.1 0 6.5 0 0 0 0 0 0 0 0 0 0
Nucleus 0 2.1 5.2 5.5 6.5 5.4 6.2 5.9 6.5 0 0 0 0 0 3.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.5 5.9 5.0 5.2 5.1 3.5 2.9 4.0 2.8 2.3 4.0 2.5 5.6 4.7 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4452 1.9052 1.7822 1.6992 1.0389 1.8342 1.7791 2.0903 2.0265 2.1517 1.7254 1.966 2.0604 2.1666 2.559 2.9983 2.3512 2.3332
Actin 0.0003 0.0115 0.0017 0.0267 0.0009 0.0055 0.0466 0.0006 0.0154 0.0004 0.0173 0.015 0.0016 0.0006 0.0011 0.0004 0.0199 0.0159
Bud 0.0002 0.0011 0.0003 0.0003 0.0001 0.004 0.0009 0.0001 0.0005 0.0001 0.0003 0.0005 0.0001 0.0001 0.0008 0.0001 0.0002 0.0006
Bud Neck 0.0002 0.0002 0.0006 0.0009 0.0011 0.0017 0.0049 0.0002 0.0034 0.0005 0.0008 0.0037 0.0002 0.0001 0.0003 0.0001 0.0026 0.0016
Bud Periphery 0.0001 0.002 0.0002 0.0003 0.0001 0.0045 0.0015 0.0001 0.0007 0.0001 0.0003 0.0022 0.0001 0 0.0008 0 0.0003 0.0014
Bud Site 0.0006 0.0044 0.0087 0.0018 0.0001 0.0004 0.0078 0.0008 0.0064 0.0029 0.0011 0.0002 0.0006 0.0006 0.0025 0.0001 0.001 0.0005
Cell Periphery 0.0001 0.0004 0.0002 0.0002 0.0001 0.0001 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.6628 0.6577 0.6373 0.5179 0.6137 0.6409 0.4308 0.5742 0.5484 0.542 0.6403 0.5654 0.8614 0.7337 0.542 0.5382 0.6263 0.6241
Cytoplasmic Foci 0.0064 0.0037 0.0079 0.0131 0.012 0.0122 0.0411 0.0082 0.0153 0.0276 0.0148 0.0085 0.0459 0.0035 0.0099 0.0046 0.0203 0.0033
Eisosomes 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0007 0 0.0003 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0071 0.0055 0.0091 0.0082 0.0078 0.0055 0.0103 0.0059 0.0069 0.0031 0.0039 0.0037 0.0063 0.0035 0.0067 0.0035 0.0038 0.0027
Endosome 0.009 0.0019 0.0077 0.0031 0.0073 0.0044 0.0321 0.0041 0.0107 0.0041 0.0173 0.0059 0.0064 0.0013 0.0172 0.0019 0.0136 0.0027
Golgi 0.0002 0.0004 0.0006 0.0022 0.0002 0.0003 0.0192 0.0005 0.0035 0.0013 0.0087 0.0007 0.0007 0.0001 0.0062 0.0001 0.0133 0.0033
Lipid Particles 0.0006 0.0003 0.0023 0.0056 0.0023 0.0006 0.0222 0.0013 0.0045 0.0094 0.0144 0.002 0.0007 0.0001 0.0009 0.0004 0.0289 0.0013
Mitochondria 0.0003 0.0003 0.0008 0.0013 0.0005 0.0004 0.0123 0.0004 0.0011 0.0005 0.0031 0.0007 0.0002 0.0001 0.0024 0.0002 0.0049 0.0036
None 0.2735 0.2754 0.279 0.3659 0.3377 0.2542 0.2876 0.3777 0.3516 0.3945 0.2117 0.2785 0.0505 0.2426 0.3855 0.4406 0.1334 0.261
Nuclear Periphery 0.0063 0.0041 0.0108 0.0055 0.0029 0.0034 0.0132 0.0029 0.0029 0.0009 0.0046 0.0071 0.004 0.0018 0.004 0.002 0.0863 0.0025
Nucleolus 0.0002 0.0002 0.0007 0.0001 0.0002 0.0008 0.0008 0.0001 0.0003 0.0006 0.0003 0.0007 0.0001 0.0001 0.0004 0.0001 0.0006 0.0002
Nucleus 0.0259 0.0283 0.0249 0.013 0.007 0.055 0.0248 0.0195 0.0166 0.0023 0.0328 0.0844 0.004 0.0097 0.0088 0.0059 0.0106 0.072
Peroxisomes 0.0001 0.0001 0.0025 0.0312 0.0002 0.0004 0.0298 0.0005 0.0048 0.0071 0.0205 0.0051 0.001 0.0001 0.0009 0.0001 0.0237 0.0008
Punctate Nuclear 0.0018 0.0015 0.0022 0.0015 0.0022 0.0034 0.0081 0.0015 0.0023 0.001 0.0053 0.0135 0.0155 0.001 0.0058 0.0008 0.0048 0.0016
Vacuole 0.0038 0.001 0.002 0.001 0.0031 0.002 0.0032 0.001 0.0035 0.0012 0.0018 0.0015 0.0006 0.0007 0.003 0.0006 0.0012 0.0006
Vacuole Periphery 0.0005 0.0002 0.0006 0.0002 0.0004 0.0002 0.0018 0.0001 0.0006 0.0002 0.0006 0.0003 0.0001 0.0001 0.0007 0.0001 0.004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3436 1.9927 4.436 4.7062 3.8535 8.9115 5.8765 3.6263 6.5068 7.3871
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg4

Atg4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available