Standard name | |
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Human Ortholog | |
Description | Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 5 | 4 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 88 | 219 | 148 | 95 | 108 | 116 | 117 | 130 | 80 | 101 | 94 | 103 | 170 | 331 | 316 | 295 | 173 | 117 | 98 | 219 | 164 | 154 | 208 | 182 |
Endoplasmic Reticulum | 1 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 1 | 0 | 1 | 0 | 2 | 9 | 9 | 5 | 0 | 0 | 3 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 5 | 7 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 3 | 6 | 4 | 9 | 4 | 0 | 2 | 3 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 15 | 5 | 5 | 18 | 14 | 58 | 119 | 96 | 100 | 180 | 145 | 189 | 0 | 2 | 4 | 8 | 5 | 1 | 0 | 1 | 5 | 0 | 3 | 1 |
Nucleus | 0 | 2 | 2 | 2 | 9 | 40 | 61 | 85 | 40 | 74 | 60 | 76 | 0 | 4 | 3 | 13 | 9 | 17 | 2 | 9 | 6 | 2 | 6 | 4 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 |
Vac/Vac Membrane | 9 | 14 | 14 | 51 | 60 | 65 | 94 | 99 | 47 | 60 | 68 | 56 | 30 | 89 | 58 | 115 | 59 | 23 | 4 | 11 | 10 | 3 | 6 | 20 |
Unique Cell Count | 102 | 235 | 167 | 154 | 171 | 226 | 328 | 353 | 217 | 327 | 290 | 317 | 180 | 389 | 350 | 374 | 215 | 144 | 114 | 256 | 198 | 164 | 235 | 229 |
Labelled Cell Count | 114 | 243 | 172 | 171 | 200 | 280 | 395 | 422 | 278 | 422 | 371 | 427 | 204 | 433 | 388 | 452 | 262 | 163 | 114 | 256 | 198 | 164 | 235 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.1 | 5.1 | 4.9 | 5.3 | 4.9 | 4.4 | 5.1 | 4.7 | 4.5 | 4.5 | 4.3 | 6.1 | 6.0 | 6.0 | 7.0 | 8.1 | 8.6 | 5.2 | 5.6 | 6.3 |
Std Deviation (1e-4) | 0.5 | 0.8 | 0.9 | 0.9 | 1.3 | 0.9 | 1.1 | 1.0 | 0.8 | 0.9 | 1.0 | 0.9 | 1.1 | 1.1 | 1.0 | 1.3 | 1.4 | 1.2 | 0.9 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.06 | -0.04 | -0.19 | -0.0 | -0.1 | -0.17 | -0.16 | -0.23 | 0.27 | 0.24 | 0.25 | 0.47 | 0.68 | 0.76 | 0.04 | 0.15 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -5.6 | -5.5 | -7.8 | -11.2 | -11.1 | -10.2 | -12.1 | -11.6 | -11.7 | 2.0 | -1.1 | 0.6 | -2.7 | -2.2 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 2.1 | 6.1 | 0 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 2.1 | 5.2 | 5.5 | 6.5 | 5.4 | 6.2 | 5.9 | 6.5 | 0 | 0 | 0 | 0 | 0 | 3.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 5.5 | 5.9 | 5.0 | 5.2 | 5.1 | 3.5 | 2.9 | 4.0 | 2.8 | 2.3 | 4.0 | 2.5 | 5.6 | 4.7 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4452 | 1.9052 | 1.7822 | 1.6992 | 1.0389 | 1.8342 | 1.7791 | 2.0903 | 2.0265 | 2.1517 | 1.7254 | 1.966 | 2.0604 | 2.1666 | 2.559 | 2.9983 | 2.3512 | 2.3332 |
Actin | 0.0003 | 0.0115 | 0.0017 | 0.0267 | 0.0009 | 0.0055 | 0.0466 | 0.0006 | 0.0154 | 0.0004 | 0.0173 | 0.015 | 0.0016 | 0.0006 | 0.0011 | 0.0004 | 0.0199 | 0.0159 |
Bud | 0.0002 | 0.0011 | 0.0003 | 0.0003 | 0.0001 | 0.004 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0006 |
Bud Neck | 0.0002 | 0.0002 | 0.0006 | 0.0009 | 0.0011 | 0.0017 | 0.0049 | 0.0002 | 0.0034 | 0.0005 | 0.0008 | 0.0037 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0026 | 0.0016 |
Bud Periphery | 0.0001 | 0.002 | 0.0002 | 0.0003 | 0.0001 | 0.0045 | 0.0015 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0022 | 0.0001 | 0 | 0.0008 | 0 | 0.0003 | 0.0014 |
Bud Site | 0.0006 | 0.0044 | 0.0087 | 0.0018 | 0.0001 | 0.0004 | 0.0078 | 0.0008 | 0.0064 | 0.0029 | 0.0011 | 0.0002 | 0.0006 | 0.0006 | 0.0025 | 0.0001 | 0.001 | 0.0005 |
Cell Periphery | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.6628 | 0.6577 | 0.6373 | 0.5179 | 0.6137 | 0.6409 | 0.4308 | 0.5742 | 0.5484 | 0.542 | 0.6403 | 0.5654 | 0.8614 | 0.7337 | 0.542 | 0.5382 | 0.6263 | 0.6241 |
Cytoplasmic Foci | 0.0064 | 0.0037 | 0.0079 | 0.0131 | 0.012 | 0.0122 | 0.0411 | 0.0082 | 0.0153 | 0.0276 | 0.0148 | 0.0085 | 0.0459 | 0.0035 | 0.0099 | 0.0046 | 0.0203 | 0.0033 |
Eisosomes | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0071 | 0.0055 | 0.0091 | 0.0082 | 0.0078 | 0.0055 | 0.0103 | 0.0059 | 0.0069 | 0.0031 | 0.0039 | 0.0037 | 0.0063 | 0.0035 | 0.0067 | 0.0035 | 0.0038 | 0.0027 |
Endosome | 0.009 | 0.0019 | 0.0077 | 0.0031 | 0.0073 | 0.0044 | 0.0321 | 0.0041 | 0.0107 | 0.0041 | 0.0173 | 0.0059 | 0.0064 | 0.0013 | 0.0172 | 0.0019 | 0.0136 | 0.0027 |
Golgi | 0.0002 | 0.0004 | 0.0006 | 0.0022 | 0.0002 | 0.0003 | 0.0192 | 0.0005 | 0.0035 | 0.0013 | 0.0087 | 0.0007 | 0.0007 | 0.0001 | 0.0062 | 0.0001 | 0.0133 | 0.0033 |
Lipid Particles | 0.0006 | 0.0003 | 0.0023 | 0.0056 | 0.0023 | 0.0006 | 0.0222 | 0.0013 | 0.0045 | 0.0094 | 0.0144 | 0.002 | 0.0007 | 0.0001 | 0.0009 | 0.0004 | 0.0289 | 0.0013 |
Mitochondria | 0.0003 | 0.0003 | 0.0008 | 0.0013 | 0.0005 | 0.0004 | 0.0123 | 0.0004 | 0.0011 | 0.0005 | 0.0031 | 0.0007 | 0.0002 | 0.0001 | 0.0024 | 0.0002 | 0.0049 | 0.0036 |
None | 0.2735 | 0.2754 | 0.279 | 0.3659 | 0.3377 | 0.2542 | 0.2876 | 0.3777 | 0.3516 | 0.3945 | 0.2117 | 0.2785 | 0.0505 | 0.2426 | 0.3855 | 0.4406 | 0.1334 | 0.261 |
Nuclear Periphery | 0.0063 | 0.0041 | 0.0108 | 0.0055 | 0.0029 | 0.0034 | 0.0132 | 0.0029 | 0.0029 | 0.0009 | 0.0046 | 0.0071 | 0.004 | 0.0018 | 0.004 | 0.002 | 0.0863 | 0.0025 |
Nucleolus | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0008 | 0.0008 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0002 |
Nucleus | 0.0259 | 0.0283 | 0.0249 | 0.013 | 0.007 | 0.055 | 0.0248 | 0.0195 | 0.0166 | 0.0023 | 0.0328 | 0.0844 | 0.004 | 0.0097 | 0.0088 | 0.0059 | 0.0106 | 0.072 |
Peroxisomes | 0.0001 | 0.0001 | 0.0025 | 0.0312 | 0.0002 | 0.0004 | 0.0298 | 0.0005 | 0.0048 | 0.0071 | 0.0205 | 0.0051 | 0.001 | 0.0001 | 0.0009 | 0.0001 | 0.0237 | 0.0008 |
Punctate Nuclear | 0.0018 | 0.0015 | 0.0022 | 0.0015 | 0.0022 | 0.0034 | 0.0081 | 0.0015 | 0.0023 | 0.001 | 0.0053 | 0.0135 | 0.0155 | 0.001 | 0.0058 | 0.0008 | 0.0048 | 0.0016 |
Vacuole | 0.0038 | 0.001 | 0.002 | 0.001 | 0.0031 | 0.002 | 0.0032 | 0.001 | 0.0035 | 0.0012 | 0.0018 | 0.0015 | 0.0006 | 0.0007 | 0.003 | 0.0006 | 0.0012 | 0.0006 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0018 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.3436 | 1.9927 | 4.436 | 4.7062 | 3.8535 | 8.9115 | 5.8765 | 3.6263 | 6.5068 | 7.3871 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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