Standard name
Human Ortholog
Description Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0.06 0.08 0.13 0.08 0.06 0.12 0.1 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0 0.07 0.05 0.09 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.13 0.18 0.09 0 0.06 0.09 0.09 0.08 0.09 0.09 0.06 0.27 0.29 0.33 0.1 0.11 0.16 0.09 0 0.09 0.33 0.3 0.31
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.21 0.05 0.07 0 0 0 0 0.05 0 0 0 0.16 0.14 0.22 0.1 0.15 0.15 0.08 0.1 0 0.09 0.1 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0 0 0.09 0.08 0.22 0.13 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0.06 0.05 0.06 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.09 0 0 0 0 0 0 0 0 0.11 0 0.05 0.05 0.15 0.08 0.05 0.06 0 0 0 0 0 0
SpindlePole 0.71 0.7 0.76 0.87 0.91 0.86 0.86 0.85 0.79 0.83 0.71 0.8 0.65 0.65 0.56 0.82 0.79 0.76 0.74 0.75 0.79 0.43 0.42 0.32
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 10 4 2 2 2 4 7 1 4 2 2 3 15 0 6 1 3 2 7 1 6 10 17
Bud 7 16 6 3 9 12 21 39 22 19 26 27 0 5 3 7 9 12 10 21 5 3 4 14
Bud Neck 4 4 9 4 9 2 2 12 7 10 6 4 2 2 2 1 6 5 1 9 1 1 1 4
Bud Site 13 8 0 5 2 14 11 9 19 15 20 23 0 0 0 9 6 3 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 55 97 40 20 12 12 24 28 21 26 21 15 104 145 231 48 53 91 35 21 21 72 68 89
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 10 6 8 0 0 0 1 2 4
Endosome 59 157 12 16 11 5 9 3 14 7 7 5 64 70 158 49 68 84 32 78 6 20 23 26
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 3 4 0 0 0 1
Mitochondria 21 15 0 0 0 7 8 11 23 24 49 36 0 0 1 10 1 9 5 14 5 11 10 20
Nucleus 2 2 0 1 2 5 1 5 4 6 0 1 0 0 0 1 1 1 0 1 0 0 1 0
Nuclear Periphery 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Nucleolus 18 32 3 7 17 11 16 13 10 17 9 15 2 3 2 15 11 18 2 3 3 2 0 0
Peroxisomes 26 63 3 5 3 8 3 4 11 4 25 10 20 24 106 36 21 33 3 13 3 0 1 1
SpindlePole 248 522 170 199 251 187 240 251 212 245 160 214 255 319 401 381 367 435 287 595 198 95 96 93
Vac/Vac Membrane 2 16 4 0 0 0 1 0 1 1 0 4 3 0 8 6 13 13 0 8 1 1 3 5
Unique Cell Count 350 741 224 230 276 217 278 297 268 294 224 268 392 494 710 467 462 570 390 793 253 221 227 289
Labelled Cell Count 462 944 251 263 319 265 341 382 345 378 325 356 454 584 915 580 564 715 390 793 253 221 227 289


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.2 6.2 6.1 5.9 5.3 5.2 5.7 4.6 4.8 4.1 4.4 6.8 6.6 6.6 7.7 8.0 7.6 6.4 6.3 6.8
Std Deviation (1e-4) 0.6 0.9 0.7 1.2 0.8 1.2 0.9 1.6 0.9 0.8 0.7 0.7 1.1 1.3 1.2 1.3 1.5 1.6 1.3 1.3 1.6
Intensity Change (Log2) – – – -0.03 -0.07 -0.23 -0.26 -0.12 -0.44 -0.38 -0.6 -0.5 0.12 0.08 0.08 0.32 0.36 0.29 0.05 0.02 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1.0 0 0 0 0
Bud 0 0.4 1.5 2.4 4.2 0 0 0 0 0 0 0 0 0 -0.5
Bud Neck -1.5 -0.5 -2.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.9 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.9 -4.9 -4.0 -3.1 -2.8 -3.4 -3.0 -2.6 -4.3 2.4 3.3 4.2 -2.8 -2.3 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.7 -0.7 -1.7 -1.2 -2.9 0 0 0 0 4.0 3.4 5.7 2.2 3.6 3.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.7 2.2 2.6 2.0 0 0 0 0 0 0 0 1.4 0 1.4
Peroxisomes 0 0 1.6 0 0 0 0 0 0 2.4 2.3 5.5 3.4 2.1 2.7
SpindlePole 2.9 4.6 2.7 3.0 2.5 0 0 0 0 -2.8 -3.0 -5.2 1.7 1.1 0.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2827 2.7752 2.946 2.2139 2.2232 2.3552 2.0415 1.8736 1.8104 2.2388 2.5261 1.7767 3.4356 2.6772 3.0092 2.7978 2.8186 2.7374
Actin 0.0004 0.001 0.0006 0.0001 0.0001 0.0001 0.0301 0.0019 0.0092 0.0044 0.0085 0.0096 0.0095 0.0034 0.0109 0.0018 0.0011 0.0014
Bud 0.0003 0.0017 0.0003 0 0 0 0.0012 0.003 0.0004 0.0003 0.004 0.0002 0.0015 0.0065 0.0058 0.0002 0.0003 0.0012
Bud Neck 0.0035 0.0135 0.0045 0.0019 0.0007 0.001 0.0063 0.0071 0.0017 0.0006 0.0015 0.0009 0.0026 0.0066 0.0087 0.0335 0.0012 0.0076
Bud Periphery 0.0003 0.0018 0.0003 0 0 0 0.0016 0.0024 0.0005 0.0003 0.0038 0.0004 0.0017 0.0039 0.0023 0.0003 0.0004 0.0009
Bud Site 0.0084 0.0325 0.0033 0.0007 0.0005 0.003 0.0064 0.0552 0.0066 0.0037 0.017 0.0005 0.0083 0.0519 0.0133 0.0011 0.001 0.0013
Cell Periphery 0.0001 0.0002 0 0 0 0 0.0005 0.0003 0.0002 0.0001 0.0003 0 0.0006 0.0004 0.0004 0.0001 0.0001 0.0001
Cytoplasm 0.0146 0.0413 0.0007 0.0011 0.0002 0.0003 0.0126 0.03 0.0025 0.0271 0.0081 0.009 0.0225 0.0411 0.0038 0.0023 0.0044 0.0019
Cytoplasmic Foci 0.2027 0.0819 0.1185 0.1976 0.2759 0.1001 0.2662 0.186 0.1353 0.0859 0.1211 0.1165 0.1838 0.1343 0.1433 0.1668 0.3005 0.1093
Eisosomes 0 0.0001 0 0 0 0 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.001 0.0021 0.0001 0.0001 0 0 0.0103 0.0018 0.0016 0.0059 0.0017 0.001 0.0039 0.0014 0.0013 0.0009 0.0038 0.0004
Endosome 0.0205 0.0347 0.0087 0.0063 0.0112 0.0037 0.0279 0.0346 0.0267 0.0187 0.0589 0.0129 0.0376 0.0242 0.0361 0.038 0.0202 0.0085
Golgi 0.0039 0.0048 0.0039 0.0015 0.0078 0.0014 0.009 0.0057 0.0142 0.0051 0.05 0.0044 0.0105 0.0054 0.0093 0.0192 0.0054 0.0032
Lipid Particles 0.0337 0.0315 0.0316 0.0089 0.1481 0.0182 0.0689 0.0313 0.0398 0.0297 0.0215 0.0317 0.0451 0.0193 0.0443 0.0332 0.0698 0.0177
Mitochondria 0.0003 0.0009 0.0005 0 0.0001 0.0001 0.0026 0.0012 0.0092 0.0016 0.0063 0.0009 0.0057 0.0012 0.0068 0.0061 0.0021 0.0061
None 0.0147 0.0488 0.0006 0.0006 0.0001 0.0003 0.0231 0.0601 0.0006 0.0065 0.0018 0.0012 0.0102 0.0112 0.0018 0.0007 0.003 0.0005
Nuclear Periphery 0.0021 0.0134 0.0017 0.0008 0.0002 0.0004 0.0101 0.004 0.0067 0.0114 0.0059 0.0038 0.0093 0.0064 0.0035 0.0089 0.0103 0.0029
Nucleolus 0.013 0.0454 0.0234 0.0087 0.0013 0.0118 0.008 0.0379 0.0356 0.0025 0.0027 0.0024 0.0037 0.0284 0.0125 0.006 0.0181 0.0088
Nucleus 0.0069 0.0269 0.008 0.0095 0.0002 0.0029 0.0173 0.0221 0.0077 0.0164 0.0046 0.0045 0.0053 0.023 0.0049 0.0023 0.0035 0.0029
Peroxisomes 0.0533 0.0282 0.0537 0.0111 0.098 0.0187 0.0412 0.0364 0.0445 0.0308 0.0134 0.0254 0.0405 0.0361 0.0365 0.0188 0.075 0.0221
Punctate Nuclear 0.6175 0.5761 0.7391 0.7504 0.4552 0.8375 0.4474 0.4699 0.6502 0.7474 0.6491 0.7741 0.5924 0.5876 0.6501 0.658 0.4765 0.8022
Vacuole 0.0027 0.0117 0.0004 0.0005 0.0001 0.0002 0.0071 0.0079 0.0048 0.0014 0.0154 0.0004 0.0037 0.0067 0.0034 0.0011 0.0018 0.0005
Vacuole Periphery 0.0003 0.0016 0.0001 0 0 0 0.0018 0.0009 0.002 0.0004 0.0043 0.0003 0.0014 0.0008 0.0009 0.0008 0.0014 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.3753 4.5195 7.5457 12.2353 8.6033 20.8432 8.7109 7.325 10.062 13.3721
Translational Efficiency 1.4906 2.2658 1.2942 1.2347 1.883 0.923 1.1461 1.4698 1.2796 1.3739

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
Localization
Cell Percentages spindle pole (75%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cnm67

Cnm67


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cnm67-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available