Standard name
Human Ortholog
Description Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.14 0.15 0.13 0.12 0.13 0.13 0.14 0.19 0.18 0.14 0.15 0.05 0 0 0 0 0.09 0.08 0.13 0.17 0.27 0.26 0.19
Bud 0.1 0.05 0.09 0 0 0 0 0 0 0.07 0.05 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.31 0.21 0.16 0.42 0.43 0.33 0.27 0.18 0.21 0.16 0.13 0.16 0.3 0.28 0.26 0 0.05 0 0.15 0 0 0.11 0.05 0
Cytoplasm 0.29 0.34 0.23 0.19 0.19 0.26 0.31 0.34 0.35 0.39 0.46 0.45 0.41 0.59 0.38 0.18 0.2 0.21 0.08 0.18 0.1 0.07 0.13 0.15
Endoplasmic Reticulum 0.35 0.49 0.44 0.52 0.38 0.44 0.42 0.46 0.45 0.37 0.34 0.33 0.34 0.38 0.49 0.06 0.07 0.07 0.58 0.49 0.46 0.41 0.36 0.42
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0.07 0.07
Golgi 0 0 0.18 0 0.16 0.07 0.08 0.1 0.06 0.07 0 0.07 0.05 0 0.1 0.73 0.6 0.55 0 0 0.05 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 31 33 25 20 27 29 37 34 47 45 18 19 6 2 2 5 3 13 14 41 36 44 57 52
Bud 13 12 15 2 8 7 11 7 11 17 6 8 10 2 2 3 0 3 2 4 2 1 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 10 5 3 3 5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 42 50 27 65 94 75 79 43 51 39 17 20 39 41 44 5 4 3 27 8 9 18 11 11
Cytoplasm 39 83 38 30 42 60 91 83 85 95 61 55 54 85 65 37 16 30 14 57 21 11 28 41
Endoplasmic Reticulum 47 118 74 81 84 100 123 112 110 90 45 40 45 55 83 13 6 10 105 153 100 66 81 114
Endosome 1 0 1 1 0 0 1 1 0 0 0 3 1 0 1 0 1 1 2 18 16 5 16 20
Golgi 4 5 31 7 35 16 23 24 15 18 2 9 6 4 17 153 49 77 1 5 10 1 3 4
Mitochondria 1 1 0 0 0 2 11 3 10 11 6 10 0 0 0 1 1 5 2 0 3 1 2 1
Nucleus 0 0 0 2 0 2 2 2 3 0 2 0 0 0 0 0 0 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 1 0 1 1 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 3 2 1 1 2 2 5 8 1 2 1 1 0 0 0 10 9 10 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 4 4
SpindlePole 3 3 3 2 6 7 3 2 1 0 0 0 4 0 5 12 4 3 0 3 6 2 6 7
Vac/Vac Membrane 0 0 0 1 2 2 1 3 0 3 2 2 0 0 0 3 1 3 2 4 0 0 2 1
Unique Cell Count 134 242 168 156 220 227 290 245 245 245 132 123 131 144 171 210 82 140 182 317 217 162 224 274
Labelled Cell Count 184 307 215 212 300 303 387 322 335 320 161 168 165 189 219 242 94 158 182 317 217 162 224 274


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.0 34.2 25.4 23.9 23.4 18.7 17.2 16.8 15.4 15.5 13.6 14.1 31.6 36.5 37.7 39.4 41.0 39.6 27.1 26.3 27.0
Std Deviation (1e-4) 4.9 6.1 4.7 4.9 4.1 3.7 3.7 3.4 3.1 3.2 3.2 2.7 6.6 7.8 8.6 11.3 10.8 10.0 5.3 5.3 5.3
Intensity Change (Log2) – – – -0.09 -0.11 -0.44 -0.56 -0.6 -0.72 -0.71 -0.9 -0.85 0.32 0.52 0.57 0.63 0.69 0.64 0.1 0.05 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.5 -0.7 -0.6 -0.6 -0.3 1.1 0.9 -0.3 0.1 -2.9 -4.2 -4.7 -4.5 -2.6 -1.5
Bud -3.1 -2.2 -2.5 -2.3 -2.7 -1.8 -0.7 -1.5 -0.8 -0.4 -2.9 -3.3 -3.4 0 -2.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 5.1 5.6 3.8 2.7 0.4 1.2 0 -0.8 0 2.8 2.6 2.2 -4.8 -2.5 -4.1
Cytoplasm -0.7 -0.9 0.9 2.0 2.5 2.6 3.5 4.3 4.0 3.5 6.6 3.1 -1.2 -0.6 -0.3
Endoplasmic Reticulum 1.4 -1.2 0 -0.3 0.3 0.2 -1.5 -1.7 -2.0 -1.7 -1.0 0.8 -8.7 -5.8 -7.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.9 -0.7 -3.5 -3.4 -2.5 -3.9 -3.4 -4.7 -2.7 -3.6 -4.4 -2.2 10.5 6.6 6.7
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.1777 35.7517 33.8499 27.5551 33.8477 31.6059 30.2707 35.1718 34.2428 33.9203 30.2747 32.8738 28.7938 34.7393 32.5773 26.4513 30.1354 31.9264
Actin 0.011 0.0013 0.0152 0.0186 0.0161 0.0122 0.0059 0.0016 0.0025 0.0013 0.0214 0.0034 0.012 0.0042 0.0021 0.0359 0.0226 0.0044
Bud 0.0001 0.0003 0.0007 0.0006 0.0003 0.0002 0.0005 0.0001 0.0006 0.0012 0.0002 0 0.0005 0.0002 0.0002 0.0009 0.0002 0.0001
Bud Neck 0.0009 0.0013 0.011 0.0088 0.0095 0.0059 0.0009 0.0011 0.0019 0.0034 0.0008 0.0004 0.0015 0.0008 0.0024 0.0025 0.0026 0.0012
Bud Periphery 0.0003 0.0003 0.0011 0.0028 0.0019 0.0009 0.0019 0.0003 0.002 0.0028 0.0006 0.0002 0.0015 0.0006 0.0009 0.0104 0.001 0.0003
Bud Site 0.0008 0.0099 0.0058 0.001 0.0006 0.0004 0.0024 0.0046 0.0014 0.0138 0.0009 0.0001 0.0038 0.003 0.0007 0.0005 0.0009 0.0002
Cell Periphery 0.1023 0.0563 0.0617 0.1056 0.0816 0.0649 0.0678 0.047 0.061 0.0979 0.0495 0.0305 0.0758 0.0411 0.0892 0.1029 0.1106 0.0403
Cytoplasm 0.0801 0.0671 0.0982 0.0387 0.1215 0.0753 0.0582 0.0195 0.0695 0.0075 0.0532 0.0142 0.0347 0.0337 0.0458 0.0358 0.0558 0.0221
Cytoplasmic Foci 0.0314 0.024 0.0311 0.0191 0.0301 0.016 0.0131 0.0168 0.0126 0.0059 0.008 0.0074 0.0257 0.0227 0.0134 0.0188 0.0097 0.0084
Eisosomes 0.0009 0.0002 0.0005 0.0013 0.0021 0.0018 0.0006 0.0003 0.0003 0.0021 0.0003 0.0002 0.0019 0.0004 0.0014 0.0012 0.005 0.0003
Endoplasmic Reticulum 0.0295 0.0239 0.0099 0.037 0.0247 0.0322 0.0261 0.01 0.0201 0.0122 0.0204 0.0165 0.0314 0.0065 0.0242 0.0427 0.0875 0.0262
Endosome 0.0205 0.0033 0.0061 0.0376 0.0067 0.0097 0.0099 0.005 0.0025 0.0026 0.004 0.0041 0.016 0.0024 0.0104 0.0101 0.0024 0.0036
Golgi 0.0107 0.0029 0.0091 0.0248 0.0137 0.0123 0.0073 0.0027 0.0032 0.0037 0.0058 0.003 0.0138 0.004 0.0056 0.0132 0.0069 0.0061
Lipid Particles 0.6744 0.7879 0.7186 0.6403 0.6794 0.7462 0.7634 0.8663 0.7942 0.8098 0.8162 0.9093 0.7286 0.8498 0.7613 0.6469 0.6546 0.8611
Mitochondria 0.006 0.0001 0.0028 0.0171 0.0001 0.0016 0.004 0.0001 0.0057 0.0011 0.0021 0.0001 0.0115 0.0001 0.0042 0.0117 0.0205 0.002
None 0.0005 0.0001 0.0006 0.0015 0.0002 0.001 0.0007 0.0001 0.0002 0.0003 0.0005 0.0001 0.0009 0.0001 0.0006 0.0005 0.0033 0.0001
Nuclear Periphery 0.0018 0.0007 0.0012 0.0082 0.0022 0.003 0.0024 0.0007 0.0009 0.0006 0.0007 0.0003 0.0037 0.001 0.0039 0.0031 0.0015 0.0016
Nucleolus 0.0005 0.001 0.0006 0.0004 0.0002 0.0006 0.0047 0.0009 0.003 0.0015 0.0003 0.0002 0.001 0.0007 0.0009 0.0004 0.0002 0.0003
Nucleus 0.0004 0.0005 0.0006 0.001 0.0003 0.0008 0.0019 0.0003 0.002 0.0026 0.0002 0.0001 0.0059 0.0197 0.0086 0.0011 0.0002 0.0124
Peroxisomes 0.0024 0.0004 0.0055 0.0005 0.0005 0.0003 0.0007 0.0003 0.0008 0.0002 0.0013 0.0003 0.0061 0.0005 0.0003 0.0241 0.0012 0.0006
Punctate Nuclear 0.0005 0.0007 0.001 0.0005 0.0004 0.0012 0.0015 0.0003 0.0003 0.0001 0.0002 0.0002 0.0025 0.001 0.0016 0.0005 0.0002 0.0004
Vacuole 0.0244 0.0177 0.0184 0.0305 0.0079 0.013 0.025 0.0215 0.0152 0.0285 0.0132 0.0094 0.0192 0.0072 0.0201 0.034 0.0127 0.0079
Vacuole Periphery 0.0007 0.0002 0.0003 0.004 0.0002 0.0004 0.0009 0.0006 0.0003 0.0006 0.0002 0.0002 0.002 0.0001 0.0023 0.003 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.938 45.7693 63.7669 76.6952 103.6381 121.1402 86.652 89.8777 103.4801 134.3238
Translational Efficiency 2.1291 2.3424 1.6649 2.1483 1.8531 2.6614 1.9811 1.8262 1.7951 1.8642

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-8

Pdr16

Pdr16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdr16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available