Standard name
Human Ortholog
Description Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0.08 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.07 0.08 0.09 0.06 – 0.09 0.09 0.09 0 0.06 0.09 0.09 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.15 0.07 0.28 0.38 0.3 0.23 0.17 0.25 – 0.08 0.07 0.13 0.24 0.27 0.28 0.45 0.35 0.28 0.21 0.2 0.24 0.17 0.18 0.17
Golgi 0.41 0.22 0.21 0.11 0.2 0 0 0.07 – 0.05 0 0 0.48 0.39 0.4 0.2 0.26 0.45 0.6 0.58 0.48 0.55 0.56 0.63
Mitochondria 0.69 0.84 0.6 0.51 0.36 0.56 0.66 0.5 – 0.73 0.74 0.68 0.34 0.34 0.19 0.08 0.08 0.06 0 0.05 0 0.07 0.05 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0.05 0.14 0.05 0.11 0.09 0.1 – 0.08 0.06 0.06 0 0 0 0.05 0.08 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.07 0.05 0.11 0.09 0.19 0.15 0.12 0.05
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0.11 0.19 0.19 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.09 0.07 0.11 0.28 0.15 0.14 0.1 – 0.09 0.05 0.07 0.14 0.17 0.15 0.1 0.1 0.09 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 17 2 1 2 1 1 12 – 8 8 7 11 8 8 1 9 8 13 10 14 3 9 12
Bud 6 4 4 2 3 6 10 14 – 19 19 36 0 2 2 2 1 2 0 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 – 2 5 0 0 0 0 1 1 0 – – – – – –
Cell Periphery 5 3 1 2 0 1 2 3 – 1 1 2 0 1 2 5 2 0 0 0 0 0 0 0
Cytoplasm 5 6 18 8 23 28 36 33 – 37 40 44 6 13 18 29 12 20 0 0 0 0 2 1
Endoplasmic Reticulum 1 2 1 2 8 6 7 13 – 11 11 5 7 2 5 11 12 2 0 1 0 3 3 4
Endosome 85 18 104 94 100 83 71 138 – 33 32 64 32 57 60 141 67 56 133 58 99 38 47 63
Golgi 238 60 80 28 65 16 18 39 – 23 15 7 65 84 84 62 50 90 376 169 200 127 149 234
Mitochondria 400 225 224 127 121 198 277 282 – 313 325 332 46 73 41 26 15 13 16 13 13 16 12 13
Nucleus 0 2 0 0 0 1 0 1 – 0 1 2 0 1 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 1 2 0 7 2 3 1 4 – 8 3 1 4 1 0 5 5 2 1 0 0 0 0 0
Nucleolus 37 10 20 35 15 38 36 59 – 33 25 29 2 0 1 16 16 14 0 1 1 0 0 0
Peroxisomes 9 4 39 6 5 12 7 17 – 5 11 11 2 9 8 26 13 11 70 25 79 34 32 17
SpindlePole 13 1 16 10 8 12 14 24 – 12 10 25 4 9 24 61 36 16 4 3 3 2 0 1
Vac/Vac Membrane 68 25 26 27 94 52 57 56 – 40 21 34 19 37 31 32 19 19 12 6 3 0 5 22
Unique Cell Count 581 269 376 247 332 356 422 563 430 437 491 135 215 211 316 191 202 633 293 421 230 268 375
Labelled Cell Count 876 379 535 349 446 457 537 697 545 527 599 198 297 284 418 259 254 633 293 421 230 268 375


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.6 10.2 9.1 8.5 8.3 7.1 6.7 7.0 – 6.2 6.4 6.5 8.9 8.8 8.2 7.3 7.5 9.0 10.7 10.4 11.4
Std Deviation (1e-4) 1.8 2.1 1.7 1.9 1.6 1.4 1.4 1.3 – 1.2 1.2 1.4 1.4 1.6 1.5 1.2 1.6 1.8 2.0 1.9 1.9
Intensity Change (Log2) – – – -0.1 -0.13 -0.35 -0.45 -0.37 – -0.56 -0.5 -0.48 -0.03 -0.05 -0.15 -0.33 -0.27 -0.01 0.23 0.19 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.4 1.6 – 2.9 2.8 4.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 1.2 1.7 2.1 0.7 – 2.1 2.4 2.4 -0.2 0.7 1.8 2.3 0.8 2.4
Endoplasmic Reticulum 0 0 0 0 2.5 – 2.7 2.7 0 0 0 0 3.2 0 0
Endosome 2.7 0.7 -1.3 -3.7 -1.1 – -7.5 -7.7 -5.4 -0.9 -0.3 0.2 4.6 1.8 0
Golgi -3.2 -0.6 -6.7 -7.3 -6.5 – -6.8 -7.9 -9.6 5.9 4.6 4.8 -0.5 1.3 5.9
Mitochondria -2.0 -6.1 -1.1 1.8 -2.9 – 4.0 4.5 2.4 -5.1 -6.0 -9.4 -14.0 -11.8 -12.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 3.8 -0.5 2.7 1.8 2.8 – 1.3 0.2 0.4 -1.9 -3.4 -3.0 -0.2 1.4 0.8
Peroxisomes -3.7 -4.9 -3.7 -5.3 -4.7 – -5.7 -4.6 -5.1 -3.3 -2.6 -2.8 -1.0 -1.4 -2.0
SpindlePole -0.1 -1.4 -0.6 -0.7 0 – -1.1 -1.6 0.6 -0.7 0 3.3 6.3 5.7 1.8
Vacuole 1.8 7.6 3.4 3.0 1.6 – 1.2 -1.3 0 2.5 3.9 3.1 1.5 1.3 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6525 6.328 5.9084 6.0076 5.3468 5.9362 5.7678 7.6593 7.2833 7.2638 6.8235 7.2874 7.1707 8.7002 8.1933 7.6902 7.7615 8.1687
Actin 0.0102 0.0096 0.005 0.006 0.0072 0.0137 0.0044 0.0046 0.0278 0.0021 0.0017 0.0048 0.0113 0.0152 0.0176 0.0236 0.0108 0.0116
Bud 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0003 0.0004 0.0002 0.0054 0.0003 0.0004 0.0006 0.0004 0.0002 0.0002 0.0001 0.0004 0.0002 0.0004 0.0002 0.0001 0.0001 0.0003
Bud Periphery 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0003 0.0008 0.0003 0.0045 0.0002 0.0002 0.0006 0.0003 0.0003 0.0001 0.0001 0.0001 0.0002 0.0005 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0 0.0001 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0011 0.0014 0.0008 0.0009 0.0007 0.0006 0.0007 0.0006 0.0006 0.0003 0.0008 0.0058 0.0005 0.0009 0.0004 0.0003 0.0002 0.0004
Cytoplasmic Foci 0.1173 0.16 0.1124 0.1012 0.1009 0.0984 0.1598 0.1415 0.0978 0.0964 0.088 0.0802 0.1346 0.1353 0.1314 0.1332 0.1218 0.1052
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0.0002
Endoplasmic Reticulum 0.0005 0.0002 0.0002 0.0006 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.0012 0.0002 0.0001 0.0009 0.0001 0.0001 0.0002 0.0006
Endosome 0.1891 0.2235 0.2029 0.2262 0.2221 0.1294 0.1376 0.1468 0.1741 0.1515 0.1632 0.1022 0.146 0.1652 0.1191 0.1399 0.1042 0.0949
Golgi 0.4469 0.4844 0.5568 0.5205 0.5576 0.5982 0.3207 0.5222 0.5504 0.5984 0.6322 0.5251 0.466 0.5576 0.5508 0.5755 0.5329 0.5933
Lipid Particles 0.0882 0.0384 0.0404 0.0326 0.0414 0.0485 0.1438 0.0998 0.0443 0.0476 0.069 0.1389 0.0686 0.0322 0.0466 0.0238 0.0678 0.0652
Mitochondria 0.0358 0.0168 0.025 0.0242 0.0069 0.0213 0.0075 0.013 0.0176 0.0163 0.0107 0.0225 0.0152 0.0126 0.0159 0.005 0.004 0.0144
None 0.0006 0.0001 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0.0002 0 0 0 0 0.0011
Nuclear Periphery 0.0016 0.0001 0.0003 0.0012 0.0006 0.0001 0.0001 0 0.0001 0 0.0006 0.0001 0.0001 0.0004 0 0 0.0001 0.0004
Nucleolus 0.0002 0.0003 0.0001 0.001 0.0001 0.0001 0.0002 0.0001 0 0 0 0 0.0001 0 0 0 0 0.0001
Nucleus 0.0005 0.0001 0.0001 0.0011 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0.0001
Peroxisomes 0.1026 0.0589 0.0518 0.063 0.0562 0.0861 0.1891 0.0684 0.0828 0.0849 0.0297 0.1169 0.1551 0.0769 0.1164 0.0973 0.1566 0.1103
Punctate Nuclear 0.0014 0.0006 0.0004 0.0035 0.0014 0.0002 0.0006 0.0003 0.0001 0.0001 0.0004 0.0002 0.0005 0.0001 0.0001 0.0001 0.0001 0.0002
Vacuole 0.0013 0.0023 0.0014 0.003 0.0017 0.001 0.0273 0.0006 0.0012 0.0006 0.0008 0.0006 0.0005 0.0005 0.0004 0.0003 0.0002 0.0005
Vacuole Periphery 0.0018 0.0017 0.0016 0.0035 0.0021 0.0012 0.0063 0.0011 0.002 0.0011 0.001 0.0015 0.0007 0.0007 0.0006 0.0006 0.0005 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.816 33.4843 26.9423 22.5427 28.9633 21.6499 34.3659 28.3567 25.4363 34.5977
Translational Efficiency 0.7544 0.6508 0.514 0.5839 0.6382 0.8113 0.6084 0.5105 0.4613 0.4985

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
Localization
Cell Percentages Golgi (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kex2

Kex2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kex2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available