Standard name
Human Ortholog
Description Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 1.0 0.96 0.95 0.97 0.88 0.87 0.79 0.77 0.73 0.69 0.98 0.99 0.97 0.83 0.89 0.91 0.95 0.93 0.93 0.96 0.87 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Mitochondria 0 0 0 0 0 0 0.06 0 0.11 0.11 0.21 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0.05 0 0.1 0 0 0 0.1 0.08 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 2 2 1 1 0 0 1 1 1 0 0 0 0 0 1 5
Bud 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 1 5 2 4 4 5 13 2 0 2 2 1 1 0 0 0 0 0 0
Cytoplasm 157 106 36 82 108 138 159 246 97 134 77 130 216 304 340 250 178 113 149 94 65 173 203 125
Endoplasmic Reticulum 2 0 0 0 1 3 8 16 2 11 2 4 3 1 2 3 5 3 1 0 0 2 3 5
Endosome 2 0 0 0 1 0 0 1 0 0 0 0 0 0 2 8 3 2 0 0 0 0 6 17
Golgi 1 0 0 0 2 0 1 0 1 1 0 1 1 0 1 9 8 3 1 0 0 0 4 17
Mitochondria 2 0 0 3 3 1 10 12 13 20 22 40 0 1 1 4 4 3 1 0 0 0 1 0
Nucleus 2 0 0 0 0 0 3 6 6 4 3 5 0 0 0 0 1 1 0 2 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 8 2 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 1 5
Vac/Vac Membrane 0 1 0 2 2 1 5 13 3 9 4 19 1 2 5 31 16 7 0 4 1 0 2 7
Unique Cell Count 161 106 36 85 114 143 181 282 123 175 106 188 220 308 350 302 201 124 157 102 71 181 233 200
Labelled Cell Count 166 107 36 87 118 144 192 300 128 185 115 213 223 308 355 320 219 137 157 102 71 181 233 200


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.4 15.9 14.3 16.1 18.1 16.3 12.7 11.6 11.3 9.7 9.4 8.7 21.7 19.0 16.4 20.5 22.4 25.9 17.4 15.5 15.3
Std Deviation (1e-4) 3.4 4.2 2.6 5.0 5.6 4.2 3.9 3.4 2.6 2.2 2.3 2.1 4.9 4.4 3.8 5.4 5.6 7.3 3.5 3.3 3.0
Intensity Change (Log2) 0.17 0.34 0.18 -0.17 -0.3 -0.34 -0.56 -0.61 -0.71 0.6 0.41 0.2 0.52 0.65 0.86 0.28 0.12 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.4 -1.1 -2.2 -2.3 -3.0 -3.2 -3.5 -3.9 -0.8 -0.7 -1.0 -2.7 -2.1 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.8764 27.6844 23.7162 24.4965 22.8656 25.4811 16.445 22.3777 17.8079 16.6245 16.4993 18.6846 22.3961 31.3686 24.8372 22.1463 23.8482 24.9291
Actin 0.0434 0.0153 0.0211 0.0351 0.003 0.0101 0.0274 0.0053 0.0277 0.022 0.0397 0.012 0.0344 0.0275 0.0371 0.0223 0.0055 0.0072
Bud 0.0023 0.003 0.0043 0.0025 0.0039 0.0038 0.0015 0.002 0.0053 0.003 0.0076 0.0011 0.006 0.0016 0.0029 0.0177 0.0031 0.0015
Bud Neck 0.0022 0.0018 0.0043 0.0019 0.0038 0.0152 0.0034 0.0018 0.0027 0.0023 0.0024 0.0068 0.0031 0.0012 0.0018 0.0043 0.0033 0.008
Bud Periphery 0.0017 0.0018 0.0026 0.0009 0.0025 0.003 0.0009 0.0006 0.003 0.0005 0.0036 0.0004 0.0031 0.0005 0.0009 0.0138 0.0007 0.001
Bud Site 0.0026 0.0103 0.0054 0.0014 0.0006 0.0014 0.0074 0.0098 0.013 0.001 0.0021 0.0005 0.0036 0.0025 0.0041 0.0017 0.0004 0.0003
Cell Periphery 0.0008 0.0006 0.0007 0.0004 0.0004 0.0004 0.0003 0.0002 0.0006 0.0001 0.0002 0.0001 0.0004 0.0001 0.0004 0.0006 0.0002 0.0001
Cytoplasm 0.6889 0.8987 0.8164 0.8561 0.868 0.8131 0.7122 0.9366 0.8257 0.8635 0.8005 0.7854 0.7501 0.9221 0.8544 0.8566 0.8847 0.8528
Cytoplasmic Foci 0.015 0.0185 0.0226 0.0226 0.0094 0.017 0.0672 0.0184 0.0278 0.0446 0.0371 0.0516 0.0245 0.0109 0.022 0.0233 0.0271 0.0243
Eisosomes 0.0006 0 0.0001 0.0002 0 0 0.0003 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0234 0.004 0.0121 0.0164 0.0067 0.0137 0.0224 0.002 0.0059 0.0034 0.0123 0.0085 0.0312 0.0021 0.0058 0.0084 0.0157 0.0099
Endosome 0.0469 0.0121 0.0356 0.0215 0.0219 0.0398 0.0818 0.0103 0.0228 0.0274 0.0358 0.0743 0.0354 0.0133 0.0173 0.0148 0.0222 0.0356
Golgi 0.0135 0.0046 0.0126 0.0096 0.0013 0.0054 0.0248 0.0037 0.0102 0.015 0.0224 0.0194 0.0071 0.0032 0.0131 0.0035 0.0028 0.0101
Lipid Particles 0.0057 0.0004 0.0023 0.0055 0.0003 0.0063 0.0057 0.0002 0.0061 0.0002 0.0011 0.0047 0.0017 0.0001 0.0042 0.0007 0.0054 0.0002
Mitochondria 0.0251 0.0059 0.0155 0.0053 0.0031 0.0046 0.0143 0.0018 0.0209 0.0032 0.0211 0.0138 0.0089 0.002 0.0042 0.0067 0.0019 0.0055
None 0.0077 0.0005 0.0013 0.0007 0.001 0.0006 0.0021 0.0003 0.0005 0.0007 0.001 0.0007 0.0033 0.0003 0.0031 0.0023 0.0044 0.0007
Nuclear Periphery 0.0427 0.0044 0.0159 0.0043 0.0168 0.0205 0.0065 0.0006 0.0022 0.0012 0.002 0.002 0.0361 0.0035 0.0057 0.0046 0.0097 0.0112
Nucleolus 0.0014 0.0002 0.0007 0.0001 0.0009 0.0002 0.0001 0.0002 0.0003 0 0.0001 0.0001 0.0004 0 0.0003 0.0001 0.0001 0.0002
Nucleus 0.0273 0.0055 0.0102 0.0053 0.0292 0.0179 0.0056 0.0017 0.0023 0.0026 0.0041 0.0037 0.0297 0.0045 0.0053 0.0082 0.0041 0.0149
Peroxisomes 0.0021 0.0019 0.0022 0.0025 0.0005 0.0024 0.0057 0.0021 0.0068 0.0075 0.0031 0.0068 0.0025 0.0013 0.0085 0.0052 0.0017 0.0018
Punctate Nuclear 0.0113 0.0004 0.0014 0.0012 0.0013 0.0012 0.0014 0.0002 0.006 0.0003 0.0004 0.0021 0.0062 0.0002 0.0023 0.0007 0.0024 0.0007
Vacuole 0.026 0.0085 0.0081 0.0045 0.0165 0.015 0.0063 0.0016 0.0078 0.0009 0.0023 0.0036 0.0081 0.0024 0.0052 0.0033 0.0031 0.0093
Vacuole Periphery 0.0094 0.0018 0.0045 0.0018 0.009 0.0083 0.0026 0.0006 0.0022 0.0006 0.0012 0.0022 0.0042 0.0006 0.0014 0.0013 0.0015 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 156.0125 126.4585 115.2863 140.4903 117.3836 150.2708 151.6079 150.4055 155.4765 142.7239
Translational Efficiency 1.2314 1.4363 1.3935 1.1152 1.2426 1.3849 1.2511 1.263 1.4794 1.352

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Lap3

Lap3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lap3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available