Standard name | |
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Human Ortholog | |
Description | Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; contains an APT1 domain that binds phosphatidylinositol-3-phosphate; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 0 | 0 | 2 |
Bud | 0 | 1 | 2 | 15 | 26 | 10 | 39 | 43 | 19 | 23 | 13 | 18 | 1 | 0 | 6 | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 3 | 3 | 1 | 5 | 10 | 3 | 4 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 2 | 1 | 2 | 5 | 5 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 0 | 3 | 3 | 3 | 3 | 2 | 1 | 2 | 2 | 0 | 0 | 1 | 2 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 277 | 140 | 209 | 116 | 199 | 190 | 319 | 399 | 169 | 186 | 137 | 195 | 134 | 117 | 136 | 97 | 107 | 121 | 262 | 144 | 169 | 81 | 119 | 144 |
Endoplasmic Reticulum | 1 | 3 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 3 | 4 | 2 | 0 | 4 | 0 | 1 | 0 |
Endosome | 8 | 0 | 0 | 5 | 36 | 17 | 45 | 22 | 11 | 12 | 11 | 8 | 5 | 8 | 9 | 21 | 25 | 17 | 12 | 4 | 2 | 2 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 5 | 5 | 35 | 16 | 57 | 97 | 131 | 79 | 98 | 83 | 101 | 4 | 1 | 7 | 3 | 2 | 4 | 6 | 3 | 21 | 1 | 2 | 6 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2 | 0 | 2 | 3 | 5 | 8 | 3 | 5 | 3 | 1 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 5 | 17 | 4 | 13 | 7 | 5 | 5 | 2 | 3 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 41 | 77 | 38 | 96 | 109 | 39 | 45 | 22 | 38 | 1 | 1 | 5 | 6 | 15 | 5 | 5 | 1 | 2 | 0 | 0 | 3 |
Vac/Vac Membrane | 2 | 1 | 0 | 0 | 1 | 0 | 3 | 8 | 0 | 4 | 0 | 6 | 0 | 0 | 1 | 6 | 11 | 4 | 7 | 2 | 4 | 2 | 3 | 1 |
Unique Cell Count | 286 | 145 | 214 | 181 | 317 | 270 | 488 | 605 | 264 | 298 | 215 | 287 | 143 | 126 | 152 | 126 | 147 | 154 | 304 | 163 | 219 | 90 | 137 | 172 |
Labelled Cell Count | 292 | 150 | 220 | 224 | 383 | 329 | 636 | 748 | 333 | 388 | 281 | 379 | 147 | 128 | 173 | 145 | 174 | 170 | 304 | 163 | 219 | 90 | 137 | 172 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 4.1 | 4.1 | 3.7 | 4.6 | 3.9 | 3.8 | 3.8 | 3.3 | 3.3 | 3.1 | 3.1 | 4.9 | 5.3 | 4.8 | 7.9 | 8.5 | 9.0 | 5.7 | 4.4 | 5.5 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.6 | 1.7 | 1.9 | 1.5 | 1.7 | 1.1 | 0.9 | 1.0 | 0.7 | 1.2 | 1.3 | 1.8 | 1.6 | 1.5 | 1.9 | 1.9 | 2.3 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.16 | -0.08 | -0.1 | -0.13 | -0.31 | -0.33 | -0.4 | -0.41 | 0.24 | 0.37 | 0.23 | 0.93 | 1.05 | 1.12 | 0.48 | 0.11 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3871 | 1.2079 | 1.1686 | 1.5579 | 1.7112 | 1.062 | 0.6939 | 1.4348 | 1.043 | 0.6758 | 0.8204 | 1.2849 | 1.7906 | 2.3008 | 2.0725 | 1.8066 | 1.593 | 2.1368 |
Actin | 0.0843 | 0.0025 | 0.0298 | 0.1356 | 0.0468 | 0.0002 | 0.0254 | 0.0005 | 0.0015 | 0.0003 | 0.0001 | 0.0022 | 0.0147 | 0.0008 | 0.0106 | 0.0296 | 0.0006 | 0.0081 |
Bud | 0.0012 | 0.0007 | 0.0009 | 0.0123 | 0.0039 | 0 | 0.0005 | 0.0031 | 0.0009 | 0.0001 | 0 | 0.0007 | 0.0024 | 0.0057 | 0.0084 | 0.0064 | 0.0013 | 0.0031 |
Bud Neck | 0.0023 | 0.0006 | 0.0005 | 0.015 | 0.0045 | 0.0003 | 0.0027 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0013 | 0.0035 | 0.0003 | 0.0036 | 0.0225 | 0.0005 | 0.0155 |
Bud Periphery | 0.0028 | 0.0008 | 0.0026 | 0.0277 | 0.0056 | 0 | 0.0005 | 0.006 | 0.0012 | 0.0001 | 0 | 0.0005 | 0.006 | 0.0038 | 0.0124 | 0.011 | 0.0013 | 0.0078 |
Bud Site | 0.0063 | 0.0174 | 0.0008 | 0.0063 | 0.012 | 0 | 0.0052 | 0.0038 | 0.0064 | 0.0001 | 0 | 0.0001 | 0.0076 | 0.0219 | 0.0154 | 0.0263 | 0.0028 | 0.0017 |
Cell Periphery | 0.0022 | 0.0004 | 0.0004 | 0.0009 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0013 | 0.0005 | 0.0008 | 0.0006 | 0.0002 | 0.0003 |
Cytoplasm | 0.1732 | 0.3469 | 0.3556 | 0.2551 | 0.2309 | 0.4386 | 0.2142 | 0.3084 | 0.2977 | 0.376 | 0.2215 | 0.2924 | 0.1092 | 0.1829 | 0.1527 | 0.0915 | 0.1837 | 0.1956 |
Cytoplasmic Foci | 0.0242 | 0.0165 | 0.0145 | 0.0177 | 0.0338 | 0.018 | 0.0581 | 0.0212 | 0.0381 | 0.0193 | 0.004 | 0.0266 | 0.0307 | 0.0104 | 0.0143 | 0.0443 | 0.0396 | 0.0414 |
Eisosomes | 0.002 | 0.0001 | 0.0007 | 0.0005 | 0.0033 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0072 | 0.0023 | 0.0058 | 0.0021 | 0.002 | 0.0011 | 0.0035 | 0.0008 | 0.0015 | 0.0019 | 0.0005 | 0.0011 | 0.0014 | 0.0004 | 0.0012 | 0.0018 | 0.0009 | 0.0015 |
Endosome | 0.0237 | 0.0119 | 0.0175 | 0.0015 | 0.0204 | 0.0092 | 0.0368 | 0.0015 | 0.0136 | 0.0087 | 0.0008 | 0.0037 | 0.0066 | 0.0012 | 0.0079 | 0.013 | 0.0079 | 0.0073 |
Golgi | 0.0084 | 0.0003 | 0.0031 | 0.0032 | 0.0187 | 0.0002 | 0.012 | 0.0004 | 0.0019 | 0.0004 | 0 | 0.0039 | 0.0022 | 0.0002 | 0.0028 | 0.0093 | 0.0003 | 0.0021 |
Lipid Particles | 0.0164 | 0.002 | 0.0094 | 0.0036 | 0.0047 | 0.0007 | 0.0158 | 0.0006 | 0.0045 | 0.0002 | 0 | 0.0031 | 0.003 | 0.0001 | 0.0033 | 0.0041 | 0.0006 | 0.0021 |
Mitochondria | 0.0133 | 0.0002 | 0.003 | 0.0015 | 0.0054 | 0.0001 | 0.0034 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0011 | 0.0083 | 0.0002 | 0.0068 | 0.0249 | 0.0003 | 0.0036 |
None | 0.5867 | 0.5863 | 0.544 | 0.5052 | 0.5928 | 0.5291 | 0.5851 | 0.6352 | 0.6158 | 0.5876 | 0.7715 | 0.6516 | 0.7839 | 0.7652 | 0.7403 | 0.6868 | 0.7566 | 0.6924 |
Nuclear Periphery | 0.0107 | 0.0005 | 0.002 | 0.0008 | 0.0008 | 0.0002 | 0.0054 | 0.0002 | 0.0006 | 0.0007 | 0.0002 | 0.0005 | 0.0005 | 0.0002 | 0.001 | 0.0007 | 0.0002 | 0.0008 |
Nucleolus | 0.0028 | 0.0005 | 0.0003 | 0.0004 | 0.0009 | 0 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0013 | 0.0007 | 0.0002 | 0.0004 |
Nucleus | 0.007 | 0.0015 | 0.001 | 0.0012 | 0.0009 | 0.0003 | 0.0069 | 0.0007 | 0.0008 | 0.0009 | 0.0004 | 0.0011 | 0.0005 | 0.0039 | 0.0038 | 0.0016 | 0.0007 | 0.0008 |
Peroxisomes | 0.0093 | 0.0001 | 0.003 | 0.0061 | 0.0052 | 0.0001 | 0.0151 | 0.0153 | 0.0103 | 0.0004 | 0 | 0.0077 | 0.0144 | 0.0001 | 0.0059 | 0.0195 | 0.0005 | 0.0093 |
Punctate Nuclear | 0.0096 | 0.002 | 0.0016 | 0.0019 | 0.0023 | 0.0004 | 0.0044 | 0.0007 | 0.0017 | 0.0012 | 0.0002 | 0.0018 | 0.0016 | 0.0013 | 0.005 | 0.0036 | 0.0007 | 0.0051 |
Vacuole | 0.0048 | 0.0061 | 0.0029 | 0.0012 | 0.0034 | 0.0011 | 0.003 | 0.0007 | 0.0015 | 0.0013 | 0.0004 | 0.0004 | 0.0013 | 0.0006 | 0.0019 | 0.0011 | 0.0008 | 0.0007 |
Vacuole Periphery | 0.0016 | 0.0006 | 0.0006 | 0.0002 | 0.0007 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.8876 | 2.8896 | 5.0542 | 8.3491 | 7.3342 | 7.3843 | 6.3128 | 7.2538 | 8.0508 | 9.4886 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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