Standard name
Human Ortholog
Description Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; contains an APT1 domain that binds phosphatidylinositol-3-phosphate; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.08 0.08 0 0.08 0.07 0.07 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.64 0.63 0.7 0.65 0.66 0.64 0.62 0.64 0.68 0.94 0.93 0.89 0.77 0.73 0.79 0.86 0.89 0.77 0.9 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.11 0.06 0.09 0 0 0 0.05 0 0 0.06 0.06 0.17 0.17 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.19 0.05 0.21 0.2 0.22 0.3 0.33 0.39 0.35 0 0 0.05 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.23 0.24 0.14 0.2 0.18 0.15 0.15 0.1 0.13 0 0 0 0.05 0.1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 3 0 0 2 0 0 1 0 0 1 2 2 0 0 2 0 0 2
Bud 0 1 2 15 26 10 39 43 19 23 13 18 1 0 6 1 0 1 0 0 2 1 2 0
Bud Neck 0 0 0 3 3 1 5 10 3 4 6 4 0 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 2 1 2 5 5 1 2 1 3 0 0 0 0 0 0
Cell Periphery 1 0 2 0 3 3 3 3 2 1 2 2 0 0 1 2 6 8 0 0 0 0 0 0
Cytoplasm 277 140 209 116 199 190 319 399 169 186 137 195 134 117 136 97 107 121 262 144 169 81 119 144
Endoplasmic Reticulum 1 3 0 0 0 4 0 0 0 0 1 0 0 0 1 4 3 4 2 0 4 0 1 0
Endosome 8 0 0 5 36 17 45 22 11 12 11 8 5 8 9 21 25 17 12 4 2 2 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 1 2 0 0 0 0
Mitochondria 2 5 5 35 16 57 97 131 79 98 83 101 4 1 7 3 2 4 6 3 21 1 2 6
Nucleus 0 0 0 1 1 0 2 2 2 0 0 0 0 0 1 1 2 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 1 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2 0 2 3 5 8 3 5 3 1 1 0 2 1 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 5 17 4 13 7 5 5 2 3 0 1 4 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 41 77 38 96 109 39 45 22 38 1 1 5 6 15 5 5 1 2 0 0 3
Vac/Vac Membrane 2 1 0 0 1 0 3 8 0 4 0 6 0 0 1 6 11 4 7 2 4 2 3 1
Unique Cell Count 286 145 214 181 317 270 488 605 264 298 215 287 143 126 152 126 147 154 304 163 219 90 137 172
Labelled Cell Count 292 150 220 224 383 329 636 748 333 388 281 379 147 128 173 145 174 170 304 163 219 90 137 172


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.1 4.1 3.7 4.6 3.9 3.8 3.8 3.3 3.3 3.1 3.1 4.9 5.3 4.8 7.9 8.5 9.0 5.7 4.4 5.5
Std Deviation (1e-4) 0.6 0.7 1.6 1.7 1.9 1.5 1.7 1.1 0.9 1.0 0.7 1.2 1.3 1.8 1.6 1.5 1.9 1.9 2.3 1.1 1.2
Intensity Change (Log2) -0.14 0.16 -0.08 -0.1 -0.13 -0.31 -0.33 -0.4 -0.41 0.24 0.37 0.23 0.93 1.05 1.12 0.48 0.11 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3871 1.2079 1.1686 1.5579 1.7112 1.062 0.6939 1.4348 1.043 0.6758 0.8204 1.2849 1.7906 2.3008 2.0725 1.8066 1.593 2.1368
Actin 0.0843 0.0025 0.0298 0.1356 0.0468 0.0002 0.0254 0.0005 0.0015 0.0003 0.0001 0.0022 0.0147 0.0008 0.0106 0.0296 0.0006 0.0081
Bud 0.0012 0.0007 0.0009 0.0123 0.0039 0 0.0005 0.0031 0.0009 0.0001 0 0.0007 0.0024 0.0057 0.0084 0.0064 0.0013 0.0031
Bud Neck 0.0023 0.0006 0.0005 0.015 0.0045 0.0003 0.0027 0.0001 0.0008 0.0002 0 0.0013 0.0035 0.0003 0.0036 0.0225 0.0005 0.0155
Bud Periphery 0.0028 0.0008 0.0026 0.0277 0.0056 0 0.0005 0.006 0.0012 0.0001 0 0.0005 0.006 0.0038 0.0124 0.011 0.0013 0.0078
Bud Site 0.0063 0.0174 0.0008 0.0063 0.012 0 0.0052 0.0038 0.0064 0.0001 0 0.0001 0.0076 0.0219 0.0154 0.0263 0.0028 0.0017
Cell Periphery 0.0022 0.0004 0.0004 0.0009 0.0009 0.0001 0.0003 0.0002 0.0001 0 0 0 0.0013 0.0005 0.0008 0.0006 0.0002 0.0003
Cytoplasm 0.1732 0.3469 0.3556 0.2551 0.2309 0.4386 0.2142 0.3084 0.2977 0.376 0.2215 0.2924 0.1092 0.1829 0.1527 0.0915 0.1837 0.1956
Cytoplasmic Foci 0.0242 0.0165 0.0145 0.0177 0.0338 0.018 0.0581 0.0212 0.0381 0.0193 0.004 0.0266 0.0307 0.0104 0.0143 0.0443 0.0396 0.0414
Eisosomes 0.002 0.0001 0.0007 0.0005 0.0033 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0005 0.0001 0.0003 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0072 0.0023 0.0058 0.0021 0.002 0.0011 0.0035 0.0008 0.0015 0.0019 0.0005 0.0011 0.0014 0.0004 0.0012 0.0018 0.0009 0.0015
Endosome 0.0237 0.0119 0.0175 0.0015 0.0204 0.0092 0.0368 0.0015 0.0136 0.0087 0.0008 0.0037 0.0066 0.0012 0.0079 0.013 0.0079 0.0073
Golgi 0.0084 0.0003 0.0031 0.0032 0.0187 0.0002 0.012 0.0004 0.0019 0.0004 0 0.0039 0.0022 0.0002 0.0028 0.0093 0.0003 0.0021
Lipid Particles 0.0164 0.002 0.0094 0.0036 0.0047 0.0007 0.0158 0.0006 0.0045 0.0002 0 0.0031 0.003 0.0001 0.0033 0.0041 0.0006 0.0021
Mitochondria 0.0133 0.0002 0.003 0.0015 0.0054 0.0001 0.0034 0.0002 0.0007 0.0003 0.0001 0.0011 0.0083 0.0002 0.0068 0.0249 0.0003 0.0036
None 0.5867 0.5863 0.544 0.5052 0.5928 0.5291 0.5851 0.6352 0.6158 0.5876 0.7715 0.6516 0.7839 0.7652 0.7403 0.6868 0.7566 0.6924
Nuclear Periphery 0.0107 0.0005 0.002 0.0008 0.0008 0.0002 0.0054 0.0002 0.0006 0.0007 0.0002 0.0005 0.0005 0.0002 0.001 0.0007 0.0002 0.0008
Nucleolus 0.0028 0.0005 0.0003 0.0004 0.0009 0 0.0007 0.0002 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0013 0.0007 0.0002 0.0004
Nucleus 0.007 0.0015 0.001 0.0012 0.0009 0.0003 0.0069 0.0007 0.0008 0.0009 0.0004 0.0011 0.0005 0.0039 0.0038 0.0016 0.0007 0.0008
Peroxisomes 0.0093 0.0001 0.003 0.0061 0.0052 0.0001 0.0151 0.0153 0.0103 0.0004 0 0.0077 0.0144 0.0001 0.0059 0.0195 0.0005 0.0093
Punctate Nuclear 0.0096 0.002 0.0016 0.0019 0.0023 0.0004 0.0044 0.0007 0.0017 0.0012 0.0002 0.0018 0.0016 0.0013 0.005 0.0036 0.0007 0.0051
Vacuole 0.0048 0.0061 0.0029 0.0012 0.0034 0.0011 0.003 0.0007 0.0015 0.0013 0.0004 0.0004 0.0013 0.0006 0.0019 0.0011 0.0008 0.0007
Vacuole Periphery 0.0016 0.0006 0.0006 0.0002 0.0007 0.0001 0.0009 0.0001 0.0003 0.0003 0.0001 0.0001 0.0002 0.0001 0.0005 0.0003 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8876 2.8896 5.0542 8.3491 7.3342 7.3843 6.3128 7.2538 8.0508 9.4886
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; contains an APT1 domain that binds phosphatidylinositol-3-phosphate; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg2

Atg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available