Standard name | |
---|---|
Human Ortholog | |
Description | RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 |
Bud Neck | 1 | 3 | 4 | 0 | 3 | 2 | 12 | 4 | 10 | 10 | 7 | 8 | 3 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 3 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 4 | 6 | 10 | 6 | 11 | 7 | 11 | 12 | 1 | 0 | 3 | 3 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 6 | 1 | 4 | 3 | 4 | 2 | 3 | 4 | 3 | 0 | 22 | 37 | 47 | 13 | 7 | 16 | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 1 | 0 | 1 | 0 | 3 | 6 | 10 | 2 | 1 | 3 | 0 | 0 | 1 | 2 | 3 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 34 | 83 | 34 | 38 | 30 | 37 | 39 | 15 | 11 | 18 | 12 | 9 | 22 | 4 | 16 | 39 | 21 | 20 | 0 | 2 | 1 | 1 | 4 | 8 |
Nucleus | 14 | 21 | 8 | 9 | 18 | 14 | 8 | 8 | 13 | 15 | 12 | 6 | 25 | 20 | 38 | 20 | 17 | 24 | 12 | 11 | 4 | 7 | 13 | 9 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 7 |
Nucleolus | 205 | 211 | 214 | 155 | 182 | 248 | 315 | 247 | 211 | 178 | 241 | 168 | 310 | 302 | 334 | 269 | 264 | 255 | 175 | 201 | 185 | 209 | 242 | 236 |
Peroxisomes | 2 | 2 | 6 | 0 | 3 | 11 | 14 | 24 | 18 | 11 | 16 | 11 | 9 | 4 | 9 | 3 | 6 | 4 | 0 | 0 | 1 | 0 | 0 | 2 |
SpindlePole | 7 | 1 | 8 | 6 | 9 | 45 | 76 | 85 | 107 | 60 | 109 | 79 | 23 | 33 | 52 | 5 | 3 | 3 | 19 | 4 | 28 | 14 | 27 | 54 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 3 | 2 | 0 | 1 | 1 | 4 | 5 | 9 |
Unique Cell Count | 210 | 223 | 229 | 168 | 203 | 265 | 348 | 276 | 255 | 212 | 268 | 184 | 358 | 355 | 432 | 308 | 285 | 291 | 211 | 225 | 233 | 250 | 310 | 352 |
Labelled Cell Count | 263 | 323 | 281 | 210 | 257 | 367 | 483 | 398 | 387 | 306 | 413 | 293 | 419 | 407 | 517 | 357 | 324 | 330 | 211 | 225 | 233 | 250 | 310 | 352 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.2 | 26.1 | 20.4 | 18.4 | 13.8 | 11.1 | 10.1 | 9.4 | 8.7 | 8.2 | 7.6 | 7.3 | 23.5 | 22.6 | 18.1 | 31.3 | 30.9 | 30.1 | 24.2 | 25.8 | 24.6 |
Std Deviation (1e-4) | 5.0 | 8.7 | 6.0 | 5.9 | 3.9 | 3.2 | 3.1 | 2.8 | 2.4 | 2.5 | 2.5 | 2.4 | 8.8 | 11.2 | 8.0 | 14.4 | 13.5 | 14.0 | 8.5 | 9.6 | 8.9 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.56 | -0.87 | -1.01 | -1.12 | -1.23 | -1.31 | -1.43 | -1.48 | 0.2 | 0.15 | -0.17 | 0.62 | 0.6 | 0.56 | 0.25 | 0.34 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 1.2 | 0 | 1.4 | 1.8 | 0.7 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.1 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 3.5 | 3.7 | 1.0 | -0.1 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.0 | 0 | -0.3 | -1.3 | -3.6 | -4.0 | -2.1 | -4.0 | -3.3 | -3.5 | -6.6 | -5.2 | -0.7 | -2.7 | -3.0 |
Nucleus | 0.9 | 2.3 | 1.0 | -0.9 | -0.4 | 0.9 | 1.7 | 0.6 | -0.1 | 1.8 | 1.2 | 2.5 | 1.5 | 1.3 | 2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.5 | -1.4 | 0.1 | -1.2 | -1.6 | -3.6 | -3.2 | -1.4 | -0.8 | -2.6 | -3.1 | -5.2 | -2.3 | -0.4 | -2.2 |
Peroxisomes | 0 | 0 | 0.9 | 0.9 | 2.9 | 2.2 | 1.4 | 1.8 | 1.7 | -0.1 | 0 | -0.4 | 0 | -0.4 | 0 |
SpindlePole | 0 | 0.5 | 4.8 | 6.1 | 7.9 | 9.9 | 7.2 | 9.7 | 9.8 | 1.5 | 2.7 | 3.6 | -1.4 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 16.8823 | 19.7259 | 18.6116 | 17.5109 | 17.0043 | 16.871 | 12.7939 | 21.7345 | 19.3166 | 16.3816 | 20.2014 | 19.1136 | 17.5858 | 19.7657 | 19.7625 | 19.7295 | 11.6797 | 18.2429 |
Actin | 0.0174 | 0 | 0.0001 | 0 | 0.0395 | 0.0076 | 0.039 | 0.001 | 0.0005 | 0 | 0 | 0.0012 | 0.0038 | 0 | 0.0016 | 0 | 0.0026 | 0.018 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0001 | 0 | 0 | 0.0014 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0039 |
Bud Periphery | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 |
Bud Site | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0016 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0002 |
Cell Periphery | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0013 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0003 |
Cytoplasm | 0.0016 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0064 | 0.0004 | 0.0003 | 0.0001 | 0 | 0 | 0.0036 | 0 | 0.0024 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0141 | 0.0012 | 0.0077 | 0.0004 | 0.0012 | 0.0025 | 0.0227 | 0.0026 | 0.006 | 0.0891 | 0.0004 | 0.003 | 0.0257 | 0.0043 | 0.0096 | 0.0099 | 0.0202 | 0.0014 |
Eisosomes | 0.0015 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0065 | 0 | 0 | 0 | 0 | 0.0002 | 0.0062 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0013 | 0 | 0.0011 | 0 | 0.0002 | 0 |
Endosome | 0.0054 | 0 | 0 | 0 | 0.0003 | 0.002 | 0.0175 | 0.0014 | 0.003 | 0.0001 | 0 | 0.0017 | 0.0154 | 0 | 0.0044 | 0 | 0.0065 | 0.0001 |
Golgi | 0.0038 | 0 | 0.0001 | 0 | 0.0013 | 0.0014 | 0.0049 | 0.001 | 0.0055 | 0.0001 | 0 | 0.0041 | 0.0042 | 0 | 0.0009 | 0 | 0.0202 | 0.0004 |
Lipid Particles | 0.0131 | 0.0019 | 0.0035 | 0.0049 | 0.0014 | 0.0061 | 0.034 | 0.0004 | 0.0123 | 0.0181 | 0.0016 | 0.0029 | 0.036 | 0.0005 | 0.0033 | 0.0105 | 0.2135 | 0.0045 |
Mitochondria | 0.0105 | 0.0001 | 0.006 | 0.0126 | 0.0064 | 0.0013 | 0.0179 | 0.0004 | 0.0086 | 0.0117 | 0.0362 | 0.0039 | 0.0048 | 0.0004 | 0.0074 | 0.0033 | 0.1241 | 0.0006 |
None | 0.0117 | 0 | 0.0011 | 0 | 0 | 0.0002 | 0.0055 | 0 | 0.0002 | 0.0008 | 0 | 0 | 0.0034 | 0 | 0.0006 | 0.0001 | 0.0004 | 0 |
Nuclear Periphery | 0.0026 | 0.0039 | 0.0002 | 0 | 0 | 0.0029 | 0.0186 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0039 | 0 | 0.0006 | 0 | 0.0004 | 0 |
Nucleolus | 0.8623 | 0.9262 | 0.9247 | 0.9073 | 0.906 | 0.9263 | 0.7286 | 0.9212 | 0.8955 | 0.8214 | 0.9232 | 0.9223 | 0.8409 | 0.9504 | 0.9147 | 0.9448 | 0.5715 | 0.9309 |
Nucleus | 0.0347 | 0.0609 | 0.0522 | 0.0734 | 0.0403 | 0.0424 | 0.0492 | 0.0685 | 0.0625 | 0.0472 | 0.0362 | 0.0526 | 0.0334 | 0.0419 | 0.0488 | 0.0287 | 0.019 | 0.0377 |
Peroxisomes | 0.0036 | 0 | 0.0006 | 0.0003 | 0.0005 | 0.0007 | 0.0119 | 0.0003 | 0.0021 | 0.0025 | 0.0009 | 0.005 | 0.0066 | 0.0001 | 0.001 | 0.0003 | 0.0152 | 0.0002 |
Punctate Nuclear | 0.0072 | 0.0052 | 0.0021 | 0.0002 | 0.0008 | 0.005 | 0.0288 | 0.0014 | 0.0024 | 0.0038 | 0.0001 | 0.0027 | 0.0133 | 0.002 | 0.0021 | 0.0004 | 0.0016 | 0.001 |
Vacuole | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0033 | 0.0004 | 0.0003 | 0.0019 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0002 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0006 | 0.0006 | 0.0003 | 0.0004 | 0.0016 | 0.0003 | 0.0003 | 0.0011 | 0.0004 | 0.0001 | 0.0016 | 0 | 0.0002 | 0.0012 | 0.0021 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 109.6331 | 98.7957 | 75.6566 | 72.6803 | 97.4375 | 83.9466 | 66.3284 | 55.2848 | 50.8186 | 73.7982 |
Translational Efficiency | 2.1117 | 1.9621 | 2.2434 | 1.7546 | 1.7604 | 1.9308 | 1.8732 | 1.7291 | 1.767 | 1.8125 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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