Standard name
Human Ortholog
Description Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 – – 0 0 – 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – – 0.06 0 – 0 0.06 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 – – 0 0.05 – 0 0 – 0.12 0.13 0.16 0.14 0.15 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.57 0.78 – – 0.78 0.87 – 0.82 0.88 – 0 0 0.05 0 0 0.07 0 0 0 0 0 0
Nucleus 0.92 0.88 0.71 0.72 – – 0.64 0.63 – 0.5 0.29 – 0.82 0.83 0.77 0.71 0.69 0.67 0.87 0.82 0.76 0.76 0.67 0.56
Nuclear Periphery 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.08
Peroxisomes 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 – – 0 0 – 0 0.06 – 0.07 0 0 0.14 0.13 0.14 0.05 0.11 0.11 0.09 0.16 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 1 – – 0 1 – 0 1 – 0 0 0 0 0 0 3 4 0 0 2 4
Bud Neck 1 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 – – 2 1 – 0 1 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 10 7 1 1 – – 1 4 – 1 0 – 12 21 29 24 25 31 4 3 0 3 2 1
Endoplasmic Reticulum 0 2 0 0 – – 0 0 – 0 0 – 0 1 1 7 9 9 0 0 0 0 0 2
Endosome 1 0 0 0 – – 0 0 – 0 0 – 1 0 1 6 6 6 2 5 0 0 1 5
Golgi 2 0 0 0 – – 0 0 – 0 0 – 0 0 0 1 0 1 3 1 0 1 1 1
Mitochondria 4 38 12 14 – – 28 72 – 28 15 – 3 0 9 6 2 13 2 6 0 0 2 2
Nucleus 345 357 15 13 – – 23 52 – 17 5 – 82 137 136 123 116 130 345 387 44 70 98 104
Nuclear Periphery 0 3 0 0 – – 0 1 – 0 0 – 0 1 0 0 0 0 1 1 0 0 0 0
Nucleolus 0 1 0 0 – – 0 1 – 0 0 – 3 1 5 2 0 1 4 4 0 1 7 13
Peroxisomes 1 0 0 0 – – 0 0 – 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 1 1 0 0 – – 0 0 – 0 0 – 1 0 2 1 0 0 5 2 1 2 4 2
Vac/Vac Membrane 11 29 0 0 – – 0 1 – 0 1 – 7 7 7 25 22 27 21 49 6 7 23 41
Unique Cell Count 374 408 21 18 36 83 34 17 100 165 176 174 168 195 397 471 59 92 147 185
Labelled Cell Count 376 438 28 29 54 133 46 23 109 168 190 195 181 220 397 471 59 92 147 185


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 5.2 3.5 3.4 – – 3.0 3.2 – 2.6 2.9 – 5.9 5.9 5.3 8.5 9.2 8.9 5.5 6.3 6.5
Std Deviation (1e-4) 0.9 0.9 0.4 0.4 – – 1.0 0.8 – 0.4 0.4 – 1.7 1.6 1.8 2.1 2.5 2.3 1.4 2.0 1.9
Intensity Change (Log2) – – – -0.02 – – -0.21 -0.12 – -0.39 -0.26 – 0.78 0.77 0.62 1.29 1.4 1.35 0.67 0.85 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Bud 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Bud Neck 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Bud Site 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Cytoplasm 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Endosome 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Golgi 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Mitochondria 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Nucleus 0.1 – – -0.6 -0.8 – -1.6 -2.6 – 1.1 1.3 0.6 -0.1 -0.2 -0.4
Nuclear Periphery 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Nucleolus 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Peroxisomes 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
SpindlePole 0 – – 0 0 – 0 0 – 0 0 0 0 0 0
Vacuole 0 – – 0 0 – 0 0 – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9232 1.6554 1.462 0.9836 1.0581 1.4346 3.5419 3.71 3.6192 3.7313 3.8267 3.649 4.3176 4.8327 3.8492 4.1611 3.6671 4.4433
Actin 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0191 0 0.0067 0.0109 0.0147 0.0057 0.0134 0.0001 0.0024 0 0.0001 0.0001
Bud 0 0 0 0 0.0001 0.0001 0.0008 0 0.0005 0.0009 0.0003 0.0001 0.0002 0.0128 0.0001 0 0 0
Bud Neck 0.0003 0.0003 0.0003 0.0003 0.0088 0.0021 0.0029 0.0002 0.0075 0.0038 0.0018 0.0015 0.0038 0.0002 0.001 0 0.0006 0.0031
Bud Periphery 0 0 0 0 0.0001 0.0001 0.0019 0 0.0009 0.0025 0.0005 0.0003 0.0016 0.0011 0.0003 0 0.0001 0.0001
Bud Site 0.0001 0.0003 0.0002 0.0001 0.0006 0.0001 0.0027 0.0002 0.0078 0.0052 0.0025 0.0003 0.0005 0.0034 0.0015 0 0.0002 0.0001
Cell Periphery 0 0 0 0 0.0001 0 0.0003 0 0.0005 0.0004 0.0002 0 0.0006 0.0001 0.0001 0 0 0
Cytoplasm 0.0093 0.0136 0.0064 0.0136 0.0036 0.0027 0.0271 0.0049 0.0107 0.0023 0.0202 0.0022 0.003 0.0196 0.0062 0.0004 0.0022 0.0039
Cytoplasmic Foci 0.0001 0.0002 0.0002 0.0001 0.0007 0.0001 0.01 0 0.0154 0.0061 0.0241 0.0034 0.0007 0.0004 0.0036 0 0.0001 0.0002
Eisosomes 0 0 0 0 0 0 0.0003 0 0.0003 0.0002 0.0006 0 0.0002 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0005 0.0005 0.0003 0.0012 0.0003 0.0003 0.0052 0.0002 0.0008 0.0005 0.0024 0.0002 0.0027 0.0007 0.0009 0 0.0002 0.0005
Endosome 0.0005 0.0007 0.0005 0.0008 0.0021 0.0005 0.0296 0.0002 0.0116 0.0249 0.0202 0.0021 0.0026 0.0009 0.0041 0 0.0003 0.0007
Golgi 0 0 0 0 0.0004 0 0.0079 0 0.0066 0.0136 0.007 0.0015 0.0008 0 0.0004 0 0 0
Lipid Particles 0 0 0.0004 0 0.0009 0 0.0217 0 0.0147 0.0124 0.0241 0.0056 0.0009 0.0001 0.0013 0 0 0.0001
Mitochondria 0.0002 0.0002 0.0002 0.0005 0.0003 0.0002 0.0134 0.0002 0.0047 0.0334 0.0041 0.0044 0.0033 0.0003 0.0007 0.0002 0.0003 0.0002
None 0.0131 0.0052 0.0054 0.0138 0.0005 0.0002 0.0364 0.002 0.0466 0.0351 0.0438 0.019 0.0016 0.012 0.0074 0.0001 0.0018 0.0057
Nuclear Periphery 0.0098 0.0089 0.0177 0.0268 0.0045 0.0041 0.0265 0.0055 0.0116 0.0068 0.0114 0.0051 0.0212 0.0262 0.0163 0.0027 0.01 0.0067
Nucleolus 0.0068 0.0103 0.0079 0.0199 0.0328 0.017 0.0119 0.0087 0.005 0.0151 0.0241 0.0171 0.0122 0.0043 0.0058 0.0018 0.0227 0.0209
Nucleus 0.9439 0.9284 0.9456 0.8918 0.9241 0.9481 0.7325 0.9695 0.8128 0.815 0.7414 0.912 0.8834 0.9085 0.9131 0.994 0.9111 0.9521
Peroxisomes 0 0 0 0 0.0001 0 0.0185 0 0.0097 0.003 0.0118 0.0034 0.0006 0 0.0015 0 0 0
Punctate Nuclear 0.0147 0.0299 0.0141 0.0303 0.0188 0.0235 0.0243 0.0081 0.0163 0.003 0.0429 0.0153 0.0436 0.0083 0.0322 0.0008 0.0491 0.0049
Vacuole 0.0005 0.0012 0.0005 0.0003 0.0009 0.0006 0.0036 0.0002 0.0085 0.0042 0.0013 0.0004 0.0021 0.0006 0.0004 0 0.0008 0.0005
Vacuole Periphery 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001 0.0033 0.0001 0.0006 0.0008 0.0006 0.0003 0.0011 0.0003 0.0002 0 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.4233 4.257 7.9901 10.4441 5.4479 8.679 9.4314 10.595 10.7488 10.3802
Translational Efficiency 0.5188 0.7661 0.636 0.4257 0.5607 0.4269 0.5625 0.4044 0.4282 0.3913

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad50

Rad50


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available