Standard name
Human Ortholog
Description Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; human homolog ATOX1 can complement yeast atx1 mutant; overexpression of human ATOX1 suppresses lysine auxotrophy of the yeast sod1 null mutant, as does overexpression of yeast ATX1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.98 0.96 0.89 – 0.73 0.71 0.65 0.63 0.64 0.99 0.99 0.98 0.93 0.96 0.95 0.96 0.89 0.95 0.93 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.13 0.05 0.23 – 0.3 0.44 0.45 0.48 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.11 0.09 0.09 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2
Bud 0 0 7 1 0 3 – 10 4 6 5 8 1 0 0 0 0 0 0 0 1 1 3 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 0 0 0 4 – 5 5 9 4 4 0 0 3 2 5 4 0 0 0 0 0 0
Cytoplasm 267 114 230 238 358 350 – 475 321 359 183 311 346 430 563 114 111 127 259 108 230 171 293 339
Endoplasmic Reticulum 1 1 0 0 0 1 – 1 0 3 0 4 2 0 2 3 7 4 0 0 0 0 0 2
Endosome 0 0 0 0 1 0 – 3 0 0 0 0 0 0 0 0 0 1 1 1 2 1 2 0
Golgi 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 2 0 5 0 1 0 0 0 3
Mitochondria 4 0 13 32 17 89 – 196 197 248 140 254 0 2 2 3 2 2 3 0 3 1 2 1
Nucleus 0 2 0 1 1 1 – 9 6 12 6 8 1 0 1 1 1 1 0 0 0 0 3 0
Nuclear Periphery 0 4 0 0 0 1 – 7 2 5 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 – 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 2 3
Vac/Vac Membrane 0 0 1 0 11 8 – 69 41 51 10 7 1 1 5 9 3 4 1 3 0 1 6 1
Unique Cell Count 272 118 237 243 373 392 655 452 554 289 489 350 433 573 123 116 134 270 122 244 184 322 362
Labelled Cell Count 276 121 253 272 388 459 775 578 693 352 600 351 433 576 134 129 148 270 122 244 184 322 362


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.6 4.1 4.5 5.7 4.8 – 4.9 4.3 4.6 4.4 4.3 7.9 9.0 10.1 12.6 12.8 12.9 5.8 6.2 6.1
Std Deviation (1e-4) 0.7 1.2 0.9 1.3 1.5 1.2 – 1.1 1.2 1.2 1.0 1.0 1.6 1.7 2.0 3.6 3.3 3.5 1.2 1.3 1.3
Intensity Change (Log2) – – – 0.13 0.46 0.21 – 0.26 0.05 0.15 0.08 0.04 0.93 1.12 1.28 1.6 1.63 1.64 0.48 0.58 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -0.7 -3.5 – -7.9 -8.1 -9.5 -9.4 -9.7 1.6 2.3 1.1 -1.9 -0.7 -1.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.5 0 – 7.6 0 0 0 0 -4.4 -4.2 0 -1.3 0 -1.9
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 5.0 4.5 4.6 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2111 3.6268 3.5074 3.0438 3.1117 3.6215 3.1176 3.9824 3.6329 3.3194 2.886 3.4539 3.108 3.7113 3.5962 3.1416 4.3651 3.7778
Actin 0.0382 0.0079 0.0178 0.0027 0.0195 0.0174 0.051 0.0008 0.0139 0.005 0.0752 0.0004 0.0331 0.0005 0.0026 0.0001 0.067 0.1013
Bud 0.0016 0.011 0.006 0.0072 0.001 0.0044 0.0006 0.0003 0.0003 0.0003 0.0004 0.0001 0.0004 0.0002 0.0005 0.0001 0.0094 0.001
Bud Neck 0.0051 0.0007 0.0018 0.0014 0.0202 0.0077 0.0006 0.002 0.0008 0.0005 0.0009 0.0008 0.0071 0.0005 0.0002 0.0002 0.0008 0.0013
Bud Periphery 0.0022 0.0035 0.0032 0.0038 0.0014 0.0039 0.0007 0.0001 0.0001 0.0005 0.0005 0 0.0004 0.0001 0.0003 0 0.0084 0.0013
Bud Site 0.0063 0.02 0.0042 0.0036 0.002 0.0027 0.0025 0.0064 0.0015 0.0007 0.0024 0.0001 0.0187 0.0185 0.0017 0 0.0673 0.0013
Cell Periphery 0.0004 0.0003 0.0003 0.0001 0.0003 0.0002 0.0001 0.0002 0 0.0001 0.0001 0 0.0006 0.0002 0.0001 0 0.0004 0.0001
Cytoplasm 0.6226 0.8358 0.7549 0.7881 0.6643 0.8029 0.7738 0.9479 0.9153 0.8494 0.7618 0.8876 0.6966 0.6195 0.7128 0.8459 0.6612 0.6719
Cytoplasmic Foci 0.049 0.0118 0.0187 0.0574 0.0269 0.016 0.012 0.004 0.0186 0.0229 0.0143 0.0053 0.0036 0.0044 0.0081 0.0091 0.0154 0.0095
Eisosomes 0.0006 0 0.0002 0 0.0004 0.0001 0.0003 0 0 0.0001 0.0003 0 0.0002 0 0 0 0.0002 0.0002
Endoplasmic Reticulum 0.0079 0.0026 0.0058 0.0026 0.011 0.0049 0.0038 0.0021 0.0025 0.0019 0.0009 0.0032 0.0052 0.0032 0.0056 0.0013 0.0044 0.0098
Endosome 0.0502 0.0018 0.0224 0.0332 0.0556 0.0149 0.0201 0.0032 0.0089 0.0377 0.0023 0.0213 0.0017 0.0014 0.0043 0.0068 0.0086 0.0176
Golgi 0.0126 0.0014 0.007 0.0038 0.018 0.0037 0.0073 0.0004 0.0023 0.0138 0.0064 0.0044 0.0006 0.0002 0.0005 0.0003 0.0031 0.0256
Lipid Particles 0.0173 0.0004 0.0062 0.0033 0.0149 0.0036 0.0077 0.0001 0.003 0.0131 0.0639 0.0006 0.0003 0.0001 0.0003 0 0.001 0.0025
Mitochondria 0.0181 0.0007 0.0045 0.0029 0.0144 0.0028 0.0038 0.0001 0.0006 0.0158 0.0024 0.0005 0.0003 0.0001 0.0009 0.0004 0.0023 0.0045
None 0.0811 0.0864 0.1033 0.0333 0.0618 0.0707 0.0662 0.0262 0.0149 0.0156 0.0083 0.0482 0.2229 0.3475 0.2505 0.1315 0.1433 0.1389
Nuclear Periphery 0.018 0.0012 0.0102 0.004 0.0291 0.0137 0.0121 0.0009 0.0033 0.002 0.0006 0.003 0.0023 0.0008 0.0037 0.0007 0.0009 0.0054
Nucleolus 0.0015 0.0005 0.0012 0.0006 0.0018 0.0005 0.0012 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001
Nucleus 0.0152 0.0061 0.0128 0.0095 0.028 0.0162 0.0157 0.003 0.0047 0.007 0.0031 0.0209 0.0037 0.0012 0.004 0.0018 0.0023 0.0028
Peroxisomes 0.0263 0.0014 0.0088 0.0124 0.0051 0.0012 0.0087 0.0001 0.0056 0.0077 0.0542 0.0001 0.0004 0 0.0006 0.0001 0.0017 0.0023
Punctate Nuclear 0.0193 0.0052 0.0074 0.0265 0.018 0.0102 0.0084 0.0004 0.0022 0.0011 0.0015 0.0025 0.0012 0.0005 0.0016 0.0003 0.0007 0.0011
Vacuole 0.0046 0.0011 0.0025 0.0026 0.0034 0.0017 0.0023 0.0015 0.001 0.0023 0.0004 0.0007 0.0005 0.0008 0.0011 0.0011 0.001 0.0008
Vacuole Periphery 0.002 0.0001 0.0012 0.0009 0.003 0.0008 0.001 0.0002 0.0003 0.0021 0.0001 0.0002 0.0001 0.0001 0.0003 0.0003 0.0002 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.6732 62.2039 92.316 99.801 64.442 104.3183 92.0952 106.1235 122.6617 130.8015
Translational Efficiency 1.83 1.5218 1.4386 1.2331 1.4302 1.4229 1.6573 2.0026 1.5073 1.6768

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; human homolog ATOX1 can complement yeast atx1 mutant; overexpression of human ATOX1 suppresses lysine auxotrophy of the yeast sod1 null mutant, as does overexpression of yeast ATX1
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atx1

Atx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available