Standard name | |
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Human Ortholog | |
Description | Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; human homolog ATOX1 can complement yeast atx1 mutant; overexpression of human ATOX1 suppresses lysine auxotrophy of the yeast sod1 null mutant, as does overexpression of yeast ATX1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 |
Bud | 0 | 0 | 7 | 1 | 0 | 3 | – | 10 | 4 | 6 | 5 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 0 | 0 | 0 | 4 | – | 5 | 5 | 9 | 4 | 4 | 0 | 0 | 3 | 2 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 267 | 114 | 230 | 238 | 358 | 350 | – | 475 | 321 | 359 | 183 | 311 | 346 | 430 | 563 | 114 | 111 | 127 | 259 | 108 | 230 | 171 | 293 | 339 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 1 | – | 1 | 0 | 3 | 0 | 4 | 2 | 0 | 2 | 3 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 5 | 0 | 1 | 0 | 0 | 0 | 3 |
Mitochondria | 4 | 0 | 13 | 32 | 17 | 89 | – | 196 | 197 | 248 | 140 | 254 | 0 | 2 | 2 | 3 | 2 | 2 | 3 | 0 | 3 | 1 | 2 | 1 |
Nucleus | 0 | 2 | 0 | 1 | 1 | 1 | – | 9 | 6 | 12 | 6 | 8 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 0 |
Nuclear Periphery | 0 | 4 | 0 | 0 | 0 | 1 | – | 7 | 2 | 5 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 11 | 8 | – | 69 | 41 | 51 | 10 | 7 | 1 | 1 | 5 | 9 | 3 | 4 | 1 | 3 | 0 | 1 | 6 | 1 |
Unique Cell Count | 272 | 118 | 237 | 243 | 373 | 392 | 655 | 452 | 554 | 289 | 489 | 350 | 433 | 573 | 123 | 116 | 134 | 270 | 122 | 244 | 184 | 322 | 362 | |
Labelled Cell Count | 276 | 121 | 253 | 272 | 388 | 459 | 775 | 578 | 693 | 352 | 600 | 351 | 433 | 576 | 134 | 129 | 148 | 270 | 122 | 244 | 184 | 322 | 362 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.6 | 4.1 | 4.5 | 5.7 | 4.8 | – | 4.9 | 4.3 | 4.6 | 4.4 | 4.3 | 7.9 | 9.0 | 10.1 | 12.6 | 12.8 | 12.9 | 5.8 | 6.2 | 6.1 |
Std Deviation (1e-4) | 0.7 | 1.2 | 0.9 | 1.3 | 1.5 | 1.2 | – | 1.1 | 1.2 | 1.2 | 1.0 | 1.0 | 1.6 | 1.7 | 2.0 | 3.6 | 3.3 | 3.5 | 1.2 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.46 | 0.21 | – | 0.26 | 0.05 | 0.15 | 0.08 | 0.04 | 0.93 | 1.12 | 1.28 | 1.6 | 1.63 | 1.64 | 0.48 | 0.58 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -0.7 | -3.5 | – | -7.9 | -8.1 | -9.5 | -9.4 | -9.7 | 1.6 | 2.3 | 1.1 | -1.9 | -0.7 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -0.5 | 0 | – | 7.6 | 0 | 0 | 0 | 0 | -4.4 | -4.2 | 0 | -1.3 | 0 | -1.9 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 5.0 | 4.5 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2111 | 3.6268 | 3.5074 | 3.0438 | 3.1117 | 3.6215 | 3.1176 | 3.9824 | 3.6329 | 3.3194 | 2.886 | 3.4539 | 3.108 | 3.7113 | 3.5962 | 3.1416 | 4.3651 | 3.7778 |
Actin | 0.0382 | 0.0079 | 0.0178 | 0.0027 | 0.0195 | 0.0174 | 0.051 | 0.0008 | 0.0139 | 0.005 | 0.0752 | 0.0004 | 0.0331 | 0.0005 | 0.0026 | 0.0001 | 0.067 | 0.1013 |
Bud | 0.0016 | 0.011 | 0.006 | 0.0072 | 0.001 | 0.0044 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0094 | 0.001 |
Bud Neck | 0.0051 | 0.0007 | 0.0018 | 0.0014 | 0.0202 | 0.0077 | 0.0006 | 0.002 | 0.0008 | 0.0005 | 0.0009 | 0.0008 | 0.0071 | 0.0005 | 0.0002 | 0.0002 | 0.0008 | 0.0013 |
Bud Periphery | 0.0022 | 0.0035 | 0.0032 | 0.0038 | 0.0014 | 0.0039 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0084 | 0.0013 |
Bud Site | 0.0063 | 0.02 | 0.0042 | 0.0036 | 0.002 | 0.0027 | 0.0025 | 0.0064 | 0.0015 | 0.0007 | 0.0024 | 0.0001 | 0.0187 | 0.0185 | 0.0017 | 0 | 0.0673 | 0.0013 |
Cell Periphery | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0001 |
Cytoplasm | 0.6226 | 0.8358 | 0.7549 | 0.7881 | 0.6643 | 0.8029 | 0.7738 | 0.9479 | 0.9153 | 0.8494 | 0.7618 | 0.8876 | 0.6966 | 0.6195 | 0.7128 | 0.8459 | 0.6612 | 0.6719 |
Cytoplasmic Foci | 0.049 | 0.0118 | 0.0187 | 0.0574 | 0.0269 | 0.016 | 0.012 | 0.004 | 0.0186 | 0.0229 | 0.0143 | 0.0053 | 0.0036 | 0.0044 | 0.0081 | 0.0091 | 0.0154 | 0.0095 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0079 | 0.0026 | 0.0058 | 0.0026 | 0.011 | 0.0049 | 0.0038 | 0.0021 | 0.0025 | 0.0019 | 0.0009 | 0.0032 | 0.0052 | 0.0032 | 0.0056 | 0.0013 | 0.0044 | 0.0098 |
Endosome | 0.0502 | 0.0018 | 0.0224 | 0.0332 | 0.0556 | 0.0149 | 0.0201 | 0.0032 | 0.0089 | 0.0377 | 0.0023 | 0.0213 | 0.0017 | 0.0014 | 0.0043 | 0.0068 | 0.0086 | 0.0176 |
Golgi | 0.0126 | 0.0014 | 0.007 | 0.0038 | 0.018 | 0.0037 | 0.0073 | 0.0004 | 0.0023 | 0.0138 | 0.0064 | 0.0044 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0.0031 | 0.0256 |
Lipid Particles | 0.0173 | 0.0004 | 0.0062 | 0.0033 | 0.0149 | 0.0036 | 0.0077 | 0.0001 | 0.003 | 0.0131 | 0.0639 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.001 | 0.0025 |
Mitochondria | 0.0181 | 0.0007 | 0.0045 | 0.0029 | 0.0144 | 0.0028 | 0.0038 | 0.0001 | 0.0006 | 0.0158 | 0.0024 | 0.0005 | 0.0003 | 0.0001 | 0.0009 | 0.0004 | 0.0023 | 0.0045 |
None | 0.0811 | 0.0864 | 0.1033 | 0.0333 | 0.0618 | 0.0707 | 0.0662 | 0.0262 | 0.0149 | 0.0156 | 0.0083 | 0.0482 | 0.2229 | 0.3475 | 0.2505 | 0.1315 | 0.1433 | 0.1389 |
Nuclear Periphery | 0.018 | 0.0012 | 0.0102 | 0.004 | 0.0291 | 0.0137 | 0.0121 | 0.0009 | 0.0033 | 0.002 | 0.0006 | 0.003 | 0.0023 | 0.0008 | 0.0037 | 0.0007 | 0.0009 | 0.0054 |
Nucleolus | 0.0015 | 0.0005 | 0.0012 | 0.0006 | 0.0018 | 0.0005 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0152 | 0.0061 | 0.0128 | 0.0095 | 0.028 | 0.0162 | 0.0157 | 0.003 | 0.0047 | 0.007 | 0.0031 | 0.0209 | 0.0037 | 0.0012 | 0.004 | 0.0018 | 0.0023 | 0.0028 |
Peroxisomes | 0.0263 | 0.0014 | 0.0088 | 0.0124 | 0.0051 | 0.0012 | 0.0087 | 0.0001 | 0.0056 | 0.0077 | 0.0542 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0017 | 0.0023 |
Punctate Nuclear | 0.0193 | 0.0052 | 0.0074 | 0.0265 | 0.018 | 0.0102 | 0.0084 | 0.0004 | 0.0022 | 0.0011 | 0.0015 | 0.0025 | 0.0012 | 0.0005 | 0.0016 | 0.0003 | 0.0007 | 0.0011 |
Vacuole | 0.0046 | 0.0011 | 0.0025 | 0.0026 | 0.0034 | 0.0017 | 0.0023 | 0.0015 | 0.001 | 0.0023 | 0.0004 | 0.0007 | 0.0005 | 0.0008 | 0.0011 | 0.0011 | 0.001 | 0.0008 |
Vacuole Periphery | 0.002 | 0.0001 | 0.0012 | 0.0009 | 0.003 | 0.0008 | 0.001 | 0.0002 | 0.0003 | 0.0021 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.6732 | 62.2039 | 92.316 | 99.801 | 64.442 | 104.3183 | 92.0952 | 106.1235 | 122.6617 | 130.8015 |
Translational Efficiency | 1.83 | 1.5218 | 1.4386 | 1.2331 | 1.4302 | 1.4229 | 1.6573 | 2.0026 | 1.5073 | 1.6768 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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