Standard name | |
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Human Ortholog | |
Description | Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; functionally redundant with BNR1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 68 | 41 | 37 | 25 | 15 | 24 | 3 | 11 | 13 | 0 | 9 | 0 | 31 | 25 | 9 | – | – | – | 51 | 45 | 74 | 48 | 68 | 54 |
Bud | 17 | 16 | 72 | 46 | 50 | 52 | 31 | 82 | 64 | 27 | 42 | 17 | 30 | 15 | 22 | – | – | – | 43 | 61 | 49 | 67 | 82 | 67 |
Bud Neck | 0 | 0 | 26 | 3 | 26 | 14 | 18 | 18 | 11 | 4 | 4 | 8 | 0 | 0 | 0 | – | – | – | 52 | 75 | 92 | 64 | 35 | 24 |
Bud Site | 23 | 29 | 34 | 40 | 24 | 43 | 17 | 17 | 23 | 6 | 12 | 3 | 13 | 0 | 1 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 18 | 11 | 17 | 14 | 7 | 11 | 10 | 14 | 28 | 26 | 22 | 12 | 25 | 43 | 47 | – | – | – | 7 | 5 | 14 | 1 | 2 | 3 |
Cytoplasm | 154 | 202 | 340 | 277 | 292 | 436 | 447 | 568 | 443 | 412 | 312 | 301 | 244 | 385 | 458 | – | – | – | 113 | 141 | 196 | 33 | 76 | 99 |
Endoplasmic Reticulum | 13 | 10 | 2 | 0 | 1 | 4 | 1 | 3 | 4 | 0 | 4 | 0 | 12 | 7 | 4 | – | – | – | 10 | 7 | 9 | 3 | 8 | 8 |
Endosome | 3 | 12 | 6 | 3 | 1 | 1 | 0 | 11 | 0 | 0 | 1 | 0 | 4 | 2 | 0 | – | – | – | 13 | 12 | 17 | 6 | 11 | 7 |
Golgi | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 1 | 0 | 1 | 2 | 0 |
Mitochondria | 6 | 16 | 28 | 75 | 57 | 160 | 101 | 243 | 246 | 92 | 221 | 90 | 1 | 2 | 4 | – | – | – | 10 | 15 | 61 | 4 | 8 | 7 |
Nucleus | 0 | 1 | 2 | 0 | 2 | 3 | 4 | 4 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 4 | 0 | 1 | 1 | 2 | 9 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 15 | 24 | 20 | 11 | 1 | 11 | 0 | 6 | 8 | 1 | 2 | 0 | 13 | 2 | 1 | – | – | – | 1 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 28 | 15 | 62 | 39 | 41 | 31 | 30 | 54 | 28 | 13 | 12 | 6 | 4 | 2 | 0 | – | – | – | 4 | 3 | 17 | 3 | 7 | 3 |
Vac/Vac Membrane | 4 | 8 | 4 | 1 | 0 | 2 | 1 | 24 | 3 | 1 | 4 | 4 | 2 | 1 | 1 | – | – | – | 3 | 14 | 17 | 1 | 7 | 4 |
Unique Cell Count | 262 | 317 | 496 | 404 | 412 | 625 | 577 | 841 | 664 | 487 | 511 | 370 | 308 | 421 | 472 | 321 | 394 | 567 | 240 | 315 | 288 | |||
Labelled Cell Count | 352 | 386 | 654 | 534 | 519 | 793 | 666 | 1064 | 874 | 585 | 646 | 445 | 380 | 484 | 547 | 321 | 394 | 567 | 240 | 315 | 288 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 5.9 | 4.5 | 4.4 | 3.4 | 3.8 | 3.0 | 3.9 | 3.4 | 3.0 | 3.4 | 2.9 | 6.3 | 6.2 | 5.3 | – | – | – | 6.3 | 6.6 | 6.9 |
Std Deviation (1e-4) | 1.0 | 0.7 | 1.1 | 1.6 | 0.9 | 0.7 | 0.6 | 1.1 | 0.6 | 0.9 | 0.9 | 1.4 | 1.0 | 1.0 | 0.9 | – | – | – | 1.0 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.41 | -0.24 | -0.58 | -0.21 | -0.42 | -0.6 | -0.41 | -0.63 | 0.47 | 0.45 | 0.24 | – | – | – | 0.47 | 0.54 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | -0.9 | -4.1 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.0 | -5.6 | -5.2 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.1 | -4.8 | -5.0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | -5.5 | -5.5 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 4.1 | 4.1 | – | – | – |
Cytoplasm | 0 | 0.8 | 0.4 | 3.3 | -0.4 | -0.7 | 5.9 | -2.5 | 4.3 | 3.3 | 8.5 | 11.6 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.9 | -4.4 | -4.2 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | -3.5 | -4.1 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.6 | -7.1 | -7.9 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1579 | 2.7634 | 2.2494 | 1.7451 | 4.2768 | 2.8792 | 2.8365 | 4.2304 | 3.4688 | 3.7531 | 3.3916 | 3.8019 | 5.2839 | 5.7789 | 5.6211 | 5.3265 | 5.2351 | 5.5461 |
Actin | 0.0644 | 0.0193 | 0.0866 | 0.0013 | 0.0088 | 0.0503 | 0.0264 | 0.0161 | 0.042 | 0.0276 | 0.0473 | 0.0231 | 0.1095 | 0.0353 | 0.0707 | 0.0828 | 0.0629 | 0.0618 |
Bud | 0.0033 | 0.0021 | 0.0799 | 0.0046 | 0.0005 | 0.0009 | 0.0101 | 0.0218 | 0.0774 | 0.0421 | 0.0174 | 0.002 | 0.0093 | 0.0243 | 0.0836 | 0.0161 | 0.0417 | 0.005 |
Bud Neck | 0.0438 | 0.0098 | 0.001 | 0.0013 | 0.0009 | 0.4449 | 0.0535 | 0.0224 | 0.0061 | 0.0033 | 0.1495 | 0.5539 | 0.0504 | 0.014 | 0.0048 | 0.0045 | 0.0831 | 0.4509 |
Bud Periphery | 0.0132 | 0.0021 | 0.1483 | 0.0021 | 0.0004 | 0.0021 | 0.0177 | 0.0154 | 0.1543 | 0.0865 | 0.0668 | 0.0054 | 0.0178 | 0.0447 | 0.169 | 0.0419 | 0.1331 | 0.0113 |
Bud Site | 0.0454 | 0.3974 | 0.0135 | 0.0003 | 0.0014 | 0.005 | 0.1892 | 0.3283 | 0.0841 | 0.0317 | 0.0335 | 0.0058 | 0.2156 | 0.3192 | 0.0904 | 0.0186 | 0.0429 | 0.0127 |
Cell Periphery | 0.0061 | 0.005 | 0.0053 | 0.0004 | 0.0003 | 0.0014 | 0.0066 | 0.004 | 0.0045 | 0.003 | 0.0087 | 0.0024 | 0.0117 | 0.0068 | 0.0051 | 0.0056 | 0.0052 | 0.0044 |
Cytoplasm | 0.198 | 0.4217 | 0.6084 | 0.8161 | 0.4025 | 0.2421 | 0.2451 | 0.4121 | 0.4097 | 0.5474 | 0.3592 | 0.2341 | 0.4458 | 0.4308 | 0.4741 | 0.7147 | 0.5088 | 0.3343 |
Cytoplasmic Foci | 0.0552 | 0.0209 | 0.0106 | 0.0028 | 0.0781 | 0.0159 | 0.1153 | 0.0358 | 0.0218 | 0.0473 | 0.0578 | 0.0562 | 0.0435 | 0.041 | 0.0209 | 0.0177 | 0.0234 | 0.0413 |
Eisosomes | 0.0014 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0018 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0017 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0006 |
Endoplasmic Reticulum | 0.0216 | 0.0019 | 0.0043 | 0.0083 | 0.004 | 0.0585 | 0.0034 | 0.0029 | 0.0046 | 0.0058 | 0.0067 | 0.0053 | 0.0072 | 0.0037 | 0.0042 | 0.0038 | 0.0056 | 0.0018 |
Endosome | 0.0531 | 0.0146 | 0.0068 | 0.0029 | 0.1121 | 0.0327 | 0.0258 | 0.0023 | 0.0107 | 0.0243 | 0.0588 | 0.0042 | 0.0124 | 0.0159 | 0.0127 | 0.0058 | 0.0207 | 0.0089 |
Golgi | 0.0422 | 0.0238 | 0.0035 | 0.0004 | 0.2395 | 0.0438 | 0.01 | 0.0013 | 0.0059 | 0.0082 | 0.0181 | 0.0043 | 0.0112 | 0.0034 | 0.0062 | 0.0043 | 0.0098 | 0.006 |
Lipid Particles | 0.1302 | 0.0066 | 0.0004 | 0.0001 | 0.0128 | 0.0183 | 0.013 | 0.0007 | 0.0061 | 0.0036 | 0.012 | 0.002 | 0.0043 | 0.0007 | 0.0004 | 0.0005 | 0.0072 | 0.0007 |
Mitochondria | 0.0841 | 0.0022 | 0.0015 | 0.0005 | 0.0295 | 0.041 | 0.0125 | 0.0003 | 0.006 | 0.0033 | 0.0187 | 0.0016 | 0.0144 | 0.0011 | 0.0031 | 0.0015 | 0.0089 | 0.0012 |
None | 0.1133 | 0.0536 | 0.0223 | 0.1532 | 0.0915 | 0.0239 | 0.2049 | 0.1267 | 0.1492 | 0.1447 | 0.1147 | 0.0903 | 0.0304 | 0.0504 | 0.044 | 0.0696 | 0.0163 | 0.0536 |
Nuclear Periphery | 0.012 | 0.0008 | 0.0008 | 0.001 | 0.0003 | 0.0024 | 0.0014 | 0.0004 | 0.0011 | 0.0014 | 0.0017 | 0.0004 | 0.0008 | 0.0006 | 0.0007 | 0.0007 | 0.0016 | 0.0002 |
Nucleolus | 0.0081 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0039 | 0.0003 | 0.0006 | 0.0007 | 0.0015 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0002 |
Nucleus | 0.0217 | 0.0027 | 0.0011 | 0.0017 | 0.0002 | 0.0014 | 0.0029 | 0.0012 | 0.002 | 0.0019 | 0.0024 | 0.0007 | 0.0012 | 0.0012 | 0.0011 | 0.0018 | 0.0016 | 0.0006 |
Peroxisomes | 0.0207 | 0.0039 | 0.0014 | 0 | 0.0142 | 0.0075 | 0.0395 | 0.0027 | 0.0042 | 0.0036 | 0.005 | 0.0053 | 0.0069 | 0.0017 | 0.0031 | 0.0012 | 0.0124 | 0.0024 |
Punctate Nuclear | 0.0562 | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0028 | 0.0115 | 0.0016 | 0.0012 | 0.0046 | 0.013 | 0.0016 | 0.0023 | 0.0014 | 0.0007 | 0.004 | 0.0065 | 0.0007 |
Vacuole | 0.0038 | 0.0073 | 0.0034 | 0.0022 | 0.0017 | 0.0017 | 0.005 | 0.003 | 0.0072 | 0.0074 | 0.0049 | 0.001 | 0.0027 | 0.0028 | 0.0041 | 0.0038 | 0.0062 | 0.0013 |
Vacuole Periphery | 0.002 | 0.0004 | 0.0004 | 0.0006 | 0.0008 | 0.0014 | 0.0008 | 0.0001 | 0.0009 | 0.0011 | 0.0022 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0016 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.7639 | 31.9706 | 29.9666 | 34.3374 | 32.9465 | 30.2883 | 36.9616 | 35.884 | 36.3534 | 40.4993 |
Translational Efficiency | 0.6092 | 0.4509 | 0.4385 | 0.4066 | 0.3751 | 0.4925 | 0.4211 | 0.3709 | 0.364 | 0.3688 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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