Standard name
Human Ortholog
Description Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.2 0.18 0.16 0.06 0.08 0.05 0.05 0.05 0.05 0 0 – – – 0.15 0.2 0.18 0.26 0.15 0.32 0.23 0.2 0.22
Bud 0.13 0.16 0.11 0.11 0.13 0.12 0.08 0.06 0.08 0.09 0.07 0.06 – – – 0.07 0.05 0.09 0.17 0.19 0.13 0.36 0.21 0.29
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.1 0.14 0.1 0.09 0.12 0.06
Bud Site 0.05 0.1 0.06 0 0.05 0 0 0 0 0 0 0 – – – 0 0.08 0 – – – – – –
Cell Periphery 0.06 0 0.09 0.05 0 0 0 0 0.05 0.05 0.07 0.05 – – – 0.05 0 0.06 0 0 0 0 0 0
Cytoplasm 0.53 0.55 0.53 0.56 0.53 0.5 0.48 0.47 0.4 0.37 0.4 0.35 – – – 0.67 0.6 0.58 0.32 0.34 0.25 0.08 0.25 0.21
Endoplasmic Reticulum 0 0 0 0.05 0 0.05 0 0.05 0 0.05 0 0 – – – 0.05 0 0.06 0 0 0.07 0 0.07 0
Endosome 0.05 0 0.09 0.06 0.13 0.08 0.05 0.05 0 0 0 0 – – – 0 0 0 0 0 0 0.07 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0.07 0.05 0.25 0.36 0.33 0.46 0.54 0.55 0.59 – – – 0.06 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.14 0.1 0.13 0.07 0.05 0 0 0 0 0 – – – 0 0.06 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.06 0 0 0 0 0 0 0 – – – 0.08 0.1 0.09 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.05 0 0.08 0.09 0.06 0.06 0.05 – – – 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 117 69 50 30 15 33 22 23 21 29 19 20 – – – 49 64 36 129 60 107 5 8 8
Bud 52 55 29 21 32 48 35 30 34 48 31 35 – – – 24 17 17 83 76 43 9 9 11
Bud Neck 2 1 0 3 1 3 1 1 2 0 1 1 – – – 2 6 1 47 55 33 2 5 2
Bud Site 19 33 16 8 13 12 16 13 9 12 7 14 – – – 9 25 6 – – – – – –
Cell Periphery 25 8 24 10 4 11 16 13 19 29 31 27 – – – 18 9 11 5 2 6 0 0 0
Cytoplasm 209 186 146 104 129 207 213 234 166 207 171 189 – – – 223 197 116 157 137 83 2 10 8
Endoplasmic Reticulum 13 10 8 10 3 22 13 23 16 27 19 23 – – – 17 8 11 16 7 22 0 3 0
Endosome 20 13 26 12 32 33 22 25 17 11 11 15 – – – 10 12 6 21 17 11 1 0 2
Golgi 10 0 4 3 1 1 0 4 2 0 0 0 – – – 5 9 3 10 4 3 0 0 1
Mitochondria 10 24 5 14 11 101 160 164 187 303 236 316 – – – 20 28 2 5 12 7 0 1 0
Nucleus 2 0 1 0 0 2 0 2 0 1 0 1 – – – 1 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 4 4 2 2 2 3 – – – 0 0 0 1 0 0 1 0 0
Nucleolus 1 6 2 2 4 10 17 14 7 13 10 13 – – – 0 0 2 0 1 0 0 0 0
Peroxisomes 18 15 39 19 32 28 24 17 10 13 9 15 – – – 12 19 6 1 0 1 0 0 0
SpindlePole 13 7 13 7 15 7 10 11 7 7 7 15 – – – 25 32 17 6 10 5 1 0 1
Vac/Vac Membrane 9 7 6 1 12 21 13 38 36 36 24 28 – – – 14 8 11 1 12 1 0 1 0
Unique Cell Count 396 340 275 187 244 411 440 497 410 559 432 539 333 327 199 492 405 332 25 43 40
Labelled Cell Count 520 434 369 244 304 539 566 616 535 738 578 715 429 437 245 492 405 332 25 43 40


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 7.9 8.2 8.0 7.6 6.8 6.4 6.7 6.5 5.9 6.1 5.9 – – – 7.0 7.7 9.8 – – –
Std Deviation (1e-4) 1.2 1.1 1.7 1.5 1.1 1.3 1.3 1.3 1.3 1.2 1.7 1.2 – – – 1.2 1.4 1.7 – – –
Intensity Change (Log2) – – – -0.03 -0.12 -0.27 -0.35 -0.29 -0.33 -0.49 -0.44 -0.47 – – – -0.23 -0.1 0.25 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 -4.1 -4.0 -5.7 -6.2 -5.5 -6.0 -6.0 -7.0 – – – -1.2 0.4 0
Bud 0.2 0.9 0.5 -1.2 -2.3 -1.0 -0.9 -1.6 -2.0 – – – -1.5 -2.5 -0.7
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site -0.7 -0.2 -1.9 -1.4 -2.2 -2.5 -2.8 -3.1 -2.3 – – – -1.9 0.9 -1.4
Cell Periphery -1.4 -3.6 -3.5 -2.9 -3.8 -2.2 -2.0 -0.8 -2.1 – – – -1.6 -3.2 -1.3
Cytoplasm 0.5 -0.1 -0.7 -1.2 -1.6 -3.2 -4.4 -3.5 -4.9 – – – 3.5 1.8 1.1
Endoplasmic Reticulum 1.3 -1.3 1.5 0 1.2 0.7 1.3 1.0 1.0 – – – 1.4 -0.4 1.4
Endosome -1.2 1.3 -0.7 -2.3 -2.4 -2.8 -4.9 -4.0 -4.1 – – – -3.4 -2.9 -2.8
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 1.1 0
Mitochondria 3.0 1.8 8.1 10.7 10.0 12.5 14.7 14.4 15.7 – – – 2.6 3.6 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 2.5 2.0 0 0 0 1.7 – – – 0 0 0
Peroxisomes -1.3 -0.4 -3.2 -4.0 -5.5 -5.8 -6.7 -6.2 -6.2 – – – -4.7 -3.5 -4.1
SpindlePole -0.5 0.7 -2.3 -1.8 -1.9 -2.3 -3.1 -2.4 -1.4 – – – 1.4 2.4 1.7
Vacuole 0 1.7 1.9 0.6 3.1 3.5 2.6 2.2 2.0 – – – 1.4 0.2 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9474 5.0397 4.5419 4.3737 4.1666 4.534 5.3884 6.4863 6.3069 5.0048 6.0436 6.5267 6.6377 7.0366 6.8366 6.6628 6.562 6.9565
Actin 0.148 0.0822 0.1435 0.0887 0.1688 0.1276 0.1617 0.0407 0.155 0.1945 0.2781 0.1172 0.2191 0.0695 0.1575 0.195 0.2429 0.1676
Bud 0.043 0.0208 0.1364 0.1055 0.0415 0.006 0.0411 0.0283 0.1351 0.0654 0.0478 0.0077 0.0323 0.0475 0.1413 0.0779 0.0384 0.0075
Bud Neck 0.0356 0.0078 0.0072 0.0041 0.0044 0.4356 0.0547 0.0092 0.0152 0.0036 0.0728 0.4882 0.035 0.0054 0.012 0.0191 0.0724 0.493
Bud Periphery 0.0542 0.021 0.1371 0.1438 0.0374 0.0042 0.0509 0.0162 0.1782 0.123 0.0703 0.008 0.0492 0.0343 0.2018 0.1358 0.0718 0.0127
Bud Site 0.1399 0.35 0.0702 0.0136 0.0116 0.0097 0.1702 0.3382 0.0886 0.0732 0.0231 0.012 0.1417 0.3257 0.0978 0.0227 0.0282 0.0064
Cell Periphery 0.0048 0.0034 0.0032 0.0019 0.0009 0.0008 0.0034 0.0032 0.0019 0.0048 0.0017 0.0009 0.0052 0.0013 0.0023 0.0018 0.0019 0.0018
Cytoplasm 0.3429 0.4141 0.3425 0.4662 0.4462 0.2041 0.2948 0.4485 0.315 0.2878 0.3354 0.1881 0.381 0.4453 0.296 0.433 0.4079 0.1877
Cytoplasmic Foci 0.0691 0.0416 0.0309 0.0454 0.1236 0.1166 0.0952 0.0453 0.0405 0.0701 0.0531 0.1073 0.0569 0.0238 0.0435 0.0459 0.0444 0.0704
Eisosomes 0.0004 0.0002 0.0002 0.0001 0.0001 0.0004 0.0005 0.0001 0.0002 0.0007 0.0003 0.0002 0.0005 0.0001 0.0002 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.0299 0.0059 0.0057 0.0093 0.0076 0.0086 0.0069 0.0023 0.0025 0.0044 0.0121 0.0023 0.0131 0.0047 0.0023 0.003 0.0034 0.0036
Endosome 0.0495 0.0182 0.0393 0.0465 0.0707 0.0297 0.0394 0.0133 0.0202 0.0231 0.0197 0.0148 0.0167 0.0191 0.0139 0.021 0.0413 0.0123
Golgi 0.0271 0.0115 0.0232 0.0244 0.0408 0.0323 0.0257 0.0073 0.0125 0.0129 0.0322 0.0113 0.0191 0.0058 0.0131 0.0194 0.0205 0.0132
Lipid Particles 0.0063 0.0013 0.0024 0.001 0.0149 0.0021 0.0078 0.0007 0.001 0.0065 0.0097 0.0024 0.0023 0.0005 0.0005 0.0004 0.0002 0.0007
Mitochondria 0.0034 0.0037 0.012 0.007 0.0077 0.0029 0.0108 0.0011 0.0032 0.0443 0.003 0.0068 0.0028 0.0011 0.0039 0.0042 0.0029 0.0014
None 0.0239 0.0021 0.0204 0.0011 0.0025 0.003 0.0092 0.0135 0.0153 0.0157 0.0078 0.0087 0.0126 0.0034 0.003 0.0058 0.0144 0.003
Nuclear Periphery 0.004 0.0008 0.0015 0.003 0.0008 0.0005 0.0013 0.0003 0.0005 0.0017 0.0009 0.0026 0.0008 0.0027 0.0006 0.0007 0.0006 0.0002
Nucleolus 0.0017 0.0007 0.0009 0.0002 0.0002 0.0002 0.0005 0.0092 0.0004 0.0011 0.0098 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002
Nucleus 0.0027 0.0016 0.0015 0.0023 0.0008 0.0006 0.0013 0.0039 0.0008 0.002 0.0017 0.0011 0.0008 0.0014 0.001 0.0009 0.0006 0.0004
Peroxisomes 0.0045 0.0077 0.0081 0.0131 0.0137 0.0098 0.016 0.0041 0.0086 0.051 0.0142 0.0175 0.0073 0.0027 0.0053 0.0072 0.0022 0.0109
Punctate Nuclear 0.0039 0.0013 0.0027 0.0008 0.0017 0.0031 0.0036 0.0011 0.0016 0.0034 0.0039 0.0011 0.0008 0.0008 0.0009 0.0008 0.0005 0.0051
Vacuole 0.0046 0.0037 0.0086 0.0183 0.0028 0.0017 0.0044 0.0133 0.0031 0.0092 0.002 0.001 0.0023 0.0039 0.0025 0.0039 0.0043 0.0012
Vacuole Periphery 0.0007 0.0004 0.0025 0.0037 0.0011 0.0005 0.0006 0.0004 0.0007 0.0017 0.0004 0.0005 0.0003 0.0007 0.0005 0.0011 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.0863 19.9006 24.0768 30.6741 32.628 39.1782 28.7605 26.866 31.2839 39.7773
Translational Efficiency 0.7898 0.7387 0.6787 0.6884 0.5889 0.6626 0.5248 0.6201 0.5956 0.5566

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
Localization
Cell Percentages cytoplasm (59%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec2

Sec2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available