Standard name | |
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Human Ortholog | |
Description | Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 19 | 100 | 43 | 16 | 21 | 31 | 19 | 16 | 19 | 20 | 12 | 54 | 48 | 52 | – | 10 | 15 | 16 | 23 | 68 | 30 | 48 | 27 |
Bud | 10 | 13 | 33 | 20 | 16 | 18 | 36 | 48 | 39 | 37 | 41 | 20 | 14 | 13 | 11 | – | 2 | 5 | 0 | 0 | 1 | 1 | 1 | 2 |
Bud Neck | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 5 | 10 | – | 1 | 0 | 9 | 10 | 7 | 10 | 16 | 19 |
Bud Site | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 33 | 37 | 120 | 84 | 45 | 68 | 92 | 38 | 66 | 47 | 62 | 64 | 166 | 185 | 202 | – | 23 | 45 | 77 | 68 | 214 | 111 | 198 | 194 |
Cytoplasm | 59 | 65 | 98 | 86 | 96 | 133 | 179 | 225 | 136 | 156 | 124 | 150 | 446 | 438 | 559 | – | 79 | 52 | 8 | 11 | 3 | 7 | 10 | 7 |
Endoplasmic Reticulum | 1 | 6 | 2 | 1 | 5 | 3 | 10 | 8 | 6 | 7 | 5 | 8 | 8 | 5 | 2 | – | 5 | 7 | 8 | 5 | 15 | 8 | 10 | 12 |
Endosome | 0 | 0 | 0 | 0 | 7 | 2 | 2 | 1 | 1 | 0 | 1 | 4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 |
Mitochondria | 2 | 1 | 26 | 23 | 21 | 91 | 138 | 238 | 171 | 224 | 216 | 172 | 2 | 0 | 0 | – | 10 | 6 | 5 | 4 | 8 | 15 | 16 | 17 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 2 | 1 | 0 | 0 | 2 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 51 | 26 | 35 | 24 | 21 | 20 | 14 | 11 | 0 | 23 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
SpindlePole | 1 | 0 | 12 | 7 | 4 | 4 | 6 | 13 | 7 | 5 | 4 | 9 | 15 | 11 | 6 | – | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 3 | 2 | 3 | 3 | 5 |
Unique Cell Count | 98 | 108 | 289 | 179 | 174 | 258 | 377 | 488 | 325 | 384 | 346 | 354 | 603 | 578 | 702 | 112 | 103 | 132 | 135 | 336 | 194 | 312 | 291 | |
Labelled Cell Count | 126 | 146 | 446 | 290 | 246 | 366 | 516 | 618 | 461 | 508 | 475 | 466 | 712 | 706 | 844 | 134 | 137 | 132 | 135 | 336 | 194 | 312 | 291 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.6 | 5.4 | 5.6 | 5.3 | 4.5 | 4.3 | 4.4 | 4.2 | 3.9 | 4.0 | 4.3 | 6.1 | 6.4 | 6.4 | 4.7 | 5.3 | 6.5 | 6.5 | 6.6 | 6.9 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.3 | 1.7 | 1.8 | 1.6 | 1.4 | 1.3 | 1.2 | 1.0 | 0.8 | 1.0 | 0.7 | 0.8 | 0.8 | 0.8 | 1.0 | 1.5 | 1.1 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.02 | -0.26 | -0.32 | -0.3 | -0.36 | -0.46 | -0.43 | -0.34 | 0.18 | 0.25 | 0.25 | -0.21 | -0.03 | 0.27 | 0.26 | 0.29 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.4 | -6.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -9.5 | -9.7 | -10.8 | – | -5.2 | -3.8 |
Bud | -0.1 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.7 | -5.7 | -6.8 | – | -3.1 | -1.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 1.1 | -3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.2 | -2.8 | -3.9 | – | -3.9 | 0.4 |
Cytoplasm | 3.0 | 4.5 | 4.2 | 3.5 | 3.3 | 2.0 | 1.8 | 0.5 | 2.2 | 11.5 | 12.0 | 13.8 | – | 6.6 | 3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 1.3 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.9 | -7.3 | -8.1 | – | 0 | -1.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | -0.9 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -10.6 | -10.4 | -11.4 | – | -4.8 | -4.6 |
SpindlePole | -0.1 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | -1.9 | -3.5 | – | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9802 | 2.3476 | 2.0001 | 1.7317 | 1.2589 | 2.4078 | 2.4262 | 3.7205 | 3.0466 | 2.4797 | 2.6873 | 3.0605 | 2.4652 | 3.2588 | 2.6512 | 2.8521 | 2.8026 | 2.6286 |
Actin | 0.063 | 0.023 | 0.0137 | 0.0082 | 0.0471 | 0.011 | 0.0365 | 0.0036 | 0.0304 | 0.0127 | 0.0402 | 0.0246 | 0.0294 | 0.0356 | 0.0192 | 0.0047 | 0.0104 | 0.025 |
Bud | 0.0033 | 0.0016 | 0.0022 | 0.0306 | 0.0025 | 0.0009 | 0.0009 | 0.0004 | 0.0011 | 0.0015 | 0.0009 | 0.0016 | 0.0009 | 0.0002 | 0.0005 | 0.0009 | 0.0006 | 0.0005 |
Bud Neck | 0.0041 | 0.0114 | 0.0116 | 0.0048 | 0.0285 | 0.0169 | 0.0067 | 0.0041 | 0.0162 | 0.0591 | 0.0176 | 0.0327 | 0.0092 | 0.0048 | 0.0114 | 0.0207 | 0.0196 | 0.0236 |
Bud Periphery | 0.0189 | 0.0051 | 0.0051 | 0.0155 | 0.0243 | 0.01 | 0.0061 | 0.0028 | 0.009 | 0.0115 | 0.0117 | 0.0148 | 0.0045 | 0.0013 | 0.0041 | 0.0094 | 0.0067 | 0.0076 |
Bud Site | 0.0081 | 0.1055 | 0.0427 | 0.007 | 0.0111 | 0.0021 | 0.0561 | 0.1056 | 0.0619 | 0.0265 | 0.005 | 0.0033 | 0.0274 | 0.0351 | 0.0391 | 0.0188 | 0.0101 | 0.0026 |
Cell Periphery | 0.0957 | 0.1616 | 0.1653 | 0.1088 | 0.258 | 0.2023 | 0.2189 | 0.3232 | 0.2925 | 0.2398 | 0.3605 | 0.2757 | 0.2096 | 0.2254 | 0.3082 | 0.2748 | 0.3106 | 0.2347 |
Cytoplasm | 0.314 | 0.5196 | 0.5558 | 0.5988 | 0.4186 | 0.5206 | 0.3941 | 0.4161 | 0.4307 | 0.5354 | 0.4842 | 0.5197 | 0.4525 | 0.5187 | 0.5052 | 0.4468 | 0.5173 | 0.5963 |
Cytoplasmic Foci | 0.0309 | 0.0089 | 0.0116 | 0.0044 | 0.028 | 0.0046 | 0.0216 | 0.0016 | 0.0038 | 0.0027 | 0.0035 | 0.0065 | 0.0132 | 0.0026 | 0.0027 | 0.0081 | 0.0059 | 0.0082 |
Eisosomes | 0.0096 | 0.0081 | 0.0103 | 0.0026 | 0.0071 | 0.0032 | 0.0117 | 0.0083 | 0.0074 | 0.0045 | 0.0066 | 0.0038 | 0.0174 | 0.0092 | 0.0147 | 0.0071 | 0.0035 | 0.0067 |
Endoplasmic Reticulum | 0.0168 | 0.0072 | 0.0097 | 0.0118 | 0.0071 | 0.0098 | 0.0077 | 0.0065 | 0.0057 | 0.0026 | 0.0023 | 0.0038 | 0.0143 | 0.0085 | 0.0045 | 0.0065 | 0.0074 | 0.0068 |
Endosome | 0.032 | 0.0012 | 0.0036 | 0.0007 | 0.0104 | 0.0137 | 0.017 | 0.0002 | 0.0019 | 0.0004 | 0.0003 | 0.0007 | 0.0025 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0012 |
Golgi | 0.01 | 0.0023 | 0.002 | 0.0025 | 0.0072 | 0.0028 | 0.0085 | 0.0005 | 0.0034 | 0.0013 | 0.0015 | 0.0012 | 0.0043 | 0.0009 | 0.0011 | 0.0007 | 0.0015 | 0.0012 |
Lipid Particles | 0.0177 | 0.0029 | 0.0091 | 0.0016 | 0.0092 | 0.0057 | 0.0223 | 0.0009 | 0.0017 | 0.0007 | 0.0007 | 0.0023 | 0.0066 | 0.0015 | 0.0016 | 0.003 | 0.0041 | 0.0046 |
Mitochondria | 0.0038 | 0.0014 | 0.0005 | 0.0022 | 0.003 | 0.0024 | 0.0039 | 0.0001 | 0.0092 | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 |
None | 0.2779 | 0.1359 | 0.1477 | 0.1927 | 0.1256 | 0.1664 | 0.1545 | 0.1235 | 0.1197 | 0.0954 | 0.0638 | 0.1045 | 0.1914 | 0.1544 | 0.0849 | 0.1923 | 0.0956 | 0.0703 |
Nuclear Periphery | 0.0363 | 0.0003 | 0.0004 | 0.0008 | 0.002 | 0.0125 | 0.0075 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0017 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0009 |
Nucleolus | 0.0009 | 0.0003 | 0.0005 | 0.0006 | 0.0011 | 0.0017 | 0.0009 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0004 |
Nucleus | 0.0082 | 0.0005 | 0.0007 | 0.0026 | 0.0015 | 0.0038 | 0.0061 | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.0003 | 0.0007 | 0.0006 | 0.0005 |
Peroxisomes | 0.0272 | 0.0006 | 0.0028 | 0.0003 | 0.0019 | 0.0005 | 0.0055 | 0.0001 | 0.0018 | 0.0002 | 0.0001 | 0.0004 | 0.0035 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0031 |
Punctate Nuclear | 0.0155 | 0.0004 | 0.0023 | 0.0011 | 0.0023 | 0.0052 | 0.006 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0009 | 0.0072 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.003 |
Vacuole | 0.0045 | 0.0021 | 0.0021 | 0.0024 | 0.003 | 0.0028 | 0.0044 | 0.0013 | 0.0017 | 0.0032 | 0.0005 | 0.002 | 0.002 | 0.0006 | 0.0014 | 0.0024 | 0.0042 | 0.0023 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.001 | 0.003 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.7049 | 9.6871 | 11.4166 | 11.8259 | 10.3308 | 5.7338 | 11.9552 | 11.4715 | 11.0627 | 14.2335 |
Translational Efficiency | 0.5783 | 0.6221 | 0.5989 | 0.4183 | 0.4391 | 1.1379 | 0.6651 | 0.4359 | 0.5009 | 0.3469 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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