Standard name
Human Ortholog
Description Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.18 0.18 0.35 0.24 0.09 0.08 0.08 0 0.05 0.05 0.06 0 0.09 0.08 0.07 0.09 0.15 0.13 0.17 0.2 0.15 0.16 0.09
Bud 0.1 0.12 0.11 0.11 0.09 0.07 0.1 0.1 0.12 0.1 0.12 0.06 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.05 0.05 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.34 0.34 0.42 0.47 0.26 0.26 0.24 0.08 0.2 0.12 0.18 0.18 0.28 0.32 0.29 0.21 0.44 0.59 0.51 0.64 0.58 0.64 0.67
Cytoplasm 0.6 0.6 0.34 0.48 0.55 0.52 0.47 0.46 0.42 0.41 0.36 0.42 0.74 0.76 0.8 0.71 0.5 0.07 0.08 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.13 0.12 0.35 0.37 0.49 0.53 0.58 0.62 0.49 0 0 0 0.09 0.06 0 0 0 0.08 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0.15 0.2 0.09 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 19 100 43 16 21 31 19 16 19 20 12 54 48 52 10 15 16 23 68 30 48 27
Bud 10 13 33 20 16 18 36 48 39 37 41 20 14 13 11 2 5 0 0 1 1 1 2
Bud Neck 2 0 0 0 0 0 0 0 0 0 0 0 7 5 10 1 0 9 10 7 10 16 19
Bud Site 0 0 2 0 1 1 1 1 3 0 0 1 0 0 0 0 0
Cell Periphery 33 37 120 84 45 68 92 38 66 47 62 64 166 185 202 23 45 77 68 214 111 198 194
Cytoplasm 59 65 98 86 96 133 179 225 136 156 124 150 446 438 559 79 52 8 11 3 7 10 7
Endoplasmic Reticulum 1 6 2 1 5 3 10 8 6 7 5 8 8 5 2 5 7 8 5 15 8 10 12
Endosome 0 0 0 0 7 2 2 1 1 0 1 4 0 0 0 0 0 0 1 2 1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 1 0 0
Mitochondria 2 1 26 23 21 91 138 238 171 224 216 172 2 0 0 10 6 5 4 8 15 16 17
Nucleus 0 1 0 0 0 0 0 4 2 0 2 1 0 0 2 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 3 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 51 26 35 24 21 20 14 11 0 23 0 0 0 0 0 0 0 1 1 0 0
SpindlePole 1 0 12 7 4 4 6 13 7 5 4 9 15 11 6 3 6 0 0 0 0 0 0
Vac/Vac Membrane 0 3 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 3 2 3 3 5
Unique Cell Count 98 108 289 179 174 258 377 488 325 384 346 354 603 578 702 112 103 132 135 336 194 312 291
Labelled Cell Count 126 146 446 290 246 366 516 618 461 508 475 466 712 706 844 134 137 132 135 336 194 312 291


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.6 5.4 5.6 5.3 4.5 4.3 4.4 4.2 3.9 4.0 4.3 6.1 6.4 6.4 4.7 5.3 6.5 6.5 6.6 6.9
Std Deviation (1e-4) 0.7 1.1 1.3 1.7 1.8 1.6 1.4 1.3 1.2 1.0 0.8 1.0 0.7 0.8 0.8 0.8 1.0 1.5 1.1 1.2 1.4
Intensity Change (Log2) 0.04 -0.02 -0.26 -0.32 -0.3 -0.36 -0.46 -0.43 -0.34 0.18 0.25 0.25 -0.21 -0.03 0.27 0.26 0.29 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.4 -6.1 0 0 0 0 0 0 0 -9.5 -9.7 -10.8 -5.2 -3.8
Bud -0.1 -0.8 0 0 0 0 0 0 0 -5.7 -5.7 -6.8 -3.1 -1.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -3.4 0 0 0 0 0 0 0 -4.2 -2.8 -3.9 -3.9 0.4
Cytoplasm 3.0 4.5 4.2 3.5 3.3 2.0 1.8 0.5 2.2 11.5 12.0 13.8 6.6 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 1.1 0 0 0 0 0 0 0 -6.9 -7.3 -8.1 0 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.9 0.7 0 0 0 0 0 0 0 -10.6 -10.4 -11.4 -4.8 -4.6
SpindlePole -0.1 -1.1 0 0 0 0 0 0 0 -1.4 -1.9 -3.5 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9802 2.3476 2.0001 1.7317 1.2589 2.4078 2.4262 3.7205 3.0466 2.4797 2.6873 3.0605 2.4652 3.2588 2.6512 2.8521 2.8026 2.6286
Actin 0.063 0.023 0.0137 0.0082 0.0471 0.011 0.0365 0.0036 0.0304 0.0127 0.0402 0.0246 0.0294 0.0356 0.0192 0.0047 0.0104 0.025
Bud 0.0033 0.0016 0.0022 0.0306 0.0025 0.0009 0.0009 0.0004 0.0011 0.0015 0.0009 0.0016 0.0009 0.0002 0.0005 0.0009 0.0006 0.0005
Bud Neck 0.0041 0.0114 0.0116 0.0048 0.0285 0.0169 0.0067 0.0041 0.0162 0.0591 0.0176 0.0327 0.0092 0.0048 0.0114 0.0207 0.0196 0.0236
Bud Periphery 0.0189 0.0051 0.0051 0.0155 0.0243 0.01 0.0061 0.0028 0.009 0.0115 0.0117 0.0148 0.0045 0.0013 0.0041 0.0094 0.0067 0.0076
Bud Site 0.0081 0.1055 0.0427 0.007 0.0111 0.0021 0.0561 0.1056 0.0619 0.0265 0.005 0.0033 0.0274 0.0351 0.0391 0.0188 0.0101 0.0026
Cell Periphery 0.0957 0.1616 0.1653 0.1088 0.258 0.2023 0.2189 0.3232 0.2925 0.2398 0.3605 0.2757 0.2096 0.2254 0.3082 0.2748 0.3106 0.2347
Cytoplasm 0.314 0.5196 0.5558 0.5988 0.4186 0.5206 0.3941 0.4161 0.4307 0.5354 0.4842 0.5197 0.4525 0.5187 0.5052 0.4468 0.5173 0.5963
Cytoplasmic Foci 0.0309 0.0089 0.0116 0.0044 0.028 0.0046 0.0216 0.0016 0.0038 0.0027 0.0035 0.0065 0.0132 0.0026 0.0027 0.0081 0.0059 0.0082
Eisosomes 0.0096 0.0081 0.0103 0.0026 0.0071 0.0032 0.0117 0.0083 0.0074 0.0045 0.0066 0.0038 0.0174 0.0092 0.0147 0.0071 0.0035 0.0067
Endoplasmic Reticulum 0.0168 0.0072 0.0097 0.0118 0.0071 0.0098 0.0077 0.0065 0.0057 0.0026 0.0023 0.0038 0.0143 0.0085 0.0045 0.0065 0.0074 0.0068
Endosome 0.032 0.0012 0.0036 0.0007 0.0104 0.0137 0.017 0.0002 0.0019 0.0004 0.0003 0.0007 0.0025 0.0002 0.0002 0.0005 0.0006 0.0012
Golgi 0.01 0.0023 0.002 0.0025 0.0072 0.0028 0.0085 0.0005 0.0034 0.0013 0.0015 0.0012 0.0043 0.0009 0.0011 0.0007 0.0015 0.0012
Lipid Particles 0.0177 0.0029 0.0091 0.0016 0.0092 0.0057 0.0223 0.0009 0.0017 0.0007 0.0007 0.0023 0.0066 0.0015 0.0016 0.003 0.0041 0.0046
Mitochondria 0.0038 0.0014 0.0005 0.0022 0.003 0.0024 0.0039 0.0001 0.0092 0.0003 0.0001 0.0003 0.0006 0.0001 0.0001 0.0001 0.0003 0.0004
None 0.2779 0.1359 0.1477 0.1927 0.1256 0.1664 0.1545 0.1235 0.1197 0.0954 0.0638 0.1045 0.1914 0.1544 0.0849 0.1923 0.0956 0.0703
Nuclear Periphery 0.0363 0.0003 0.0004 0.0008 0.002 0.0125 0.0075 0.0004 0.0007 0.0001 0.0001 0.0002 0.0017 0.0003 0.0002 0.0004 0.0003 0.0009
Nucleolus 0.0009 0.0003 0.0005 0.0006 0.0011 0.0017 0.0009 0.0002 0.0002 0.0006 0.0001 0.0002 0.0007 0.0001 0.0001 0.0005 0.0003 0.0004
Nucleus 0.0082 0.0005 0.0007 0.0026 0.0015 0.0038 0.0061 0.0003 0.0005 0.0009 0.0001 0.0005 0.0009 0.0002 0.0003 0.0007 0.0006 0.0005
Peroxisomes 0.0272 0.0006 0.0028 0.0003 0.0019 0.0005 0.0055 0.0001 0.0018 0.0002 0.0001 0.0004 0.0035 0.0002 0.0004 0.0001 0.0001 0.0031
Punctate Nuclear 0.0155 0.0004 0.0023 0.0011 0.0023 0.0052 0.006 0.0001 0.0005 0.0002 0.0001 0.0009 0.0072 0.0001 0.0001 0.0013 0.0002 0.003
Vacuole 0.0045 0.0021 0.0021 0.0024 0.003 0.0028 0.0044 0.0013 0.0017 0.0032 0.0005 0.002 0.002 0.0006 0.0014 0.0024 0.0042 0.0023
Vacuole Periphery 0.0015 0.0001 0.0001 0.0002 0.0005 0.001 0.003 0 0.0002 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7049 9.6871 11.4166 11.8259 10.3308 5.7338 11.9552 11.4715 11.0627 14.2335
Translational Efficiency 0.5783 0.6221 0.5989 0.4183 0.4391 1.1379 0.6651 0.4359 0.5009 0.3469

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1
Localization
Cell Percentages cell periphery (52%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Bor1

Bor1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bor1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available