Standard name
Human Ortholog
Description L-homoserine-O-acetyltransferase; catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.99 0.97 0.97 0.98 0.92 0.92 0.92 0.85 0.88 0.7 0.71 0.99 1.0 0.99 – 0.96 0.96 0.88 0.91 0.8 0.91 0.85 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.09 0.05 0.16 0.24 0.24 0.35 0.39 0.49 0.47 0 0 0 – 0 0 0.06 0.05 0.05 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 4 3
Bud 0 3 1 0 1 0 2 5 4 1 1 6 0 1 2 – 0 0 1 0 0 1 9 12
Bud Neck 0 0 0 0 0 1 2 4 1 2 0 1 1 1 0 – 0 0 0 0 0 1 2 3
Bud Site 0 0 0 0 0 0 0 2 1 1 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 0 0 3 3 1 1 0 1 1 – 3 6 0 0 1 1 2 2
Cytoplasm 156 206 73 94 128 124 209 270 161 164 92 138 427 581 634 – 186 140 151 197 59 286 375 352
Endoplasmic Reticulum 0 0 1 3 1 1 0 1 0 1 0 0 2 2 1 – 14 11 0 0 2 0 2 2
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 2 1 1 0 0 0 2 4
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – 1 1 0 0 0 0 1 4
Mitochondria 19 6 3 9 7 22 54 69 67 73 64 91 1 1 1 – 5 0 10 10 3 10 20 12
Nucleus 0 0 0 1 2 2 1 6 3 0 0 0 0 0 0 – 1 1 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 6 3 3 5 0 0 0 – 1 0 2 0 0 3 9 5
Vac/Vac Membrane 2 0 0 0 0 0 0 1 1 0 2 0 0 0 0 – 4 1 3 1 1 2 8 9
Unique Cell Count 170 208 75 97 130 135 228 292 189 187 131 195 431 583 639 193 146 173 216 75 314 444 427
Labelled Cell Count 178 216 78 108 143 151 268 360 247 248 164 244 431 587 641 218 161 173 216 75 314 444 427


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.7 3.6 3.9 3.7 3.6 2.5 3.2 3.1 2.5 2.8 2.6 4.2 4.0 4.0 4.9 6.1 6.0 3.9 4.0 4.8
Std Deviation (1e-4) 1.4 0.7 1.2 2.4 1.5 2.4 1.1 1.5 2.1 1.3 1.6 1.7 1.1 1.0 1.1 1.3 2.0 1.7 1.1 1.3 1.6
Intensity Change (Log2) – – – 0.12 0.03 -0.0 -0.53 -0.18 -0.23 -0.52 -0.37 -0.47 0.21 0.14 0.14 0.43 0.77 0.73 0.11 0.16 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.4298 12.6477 11.7945 11.2809 12.3463 11.9676 6.6654 10.0693 8.296 7.7896 5.7913 8.9864 7.5989 9.9722 9.3031 10.3347 11.683 9.8654
Actin 0.0003 0.001 0.0024 0.0012 0.0007 0.001 0.0447 0.0034 0.0189 0.017 0.0034 0.0038 0.0282 0.0019 0.0225 0.0009 0.003 0.0049
Bud 0.0002 0.0024 0.001 0.0014 0.0006 0.0004 0.001 0.0013 0.0077 0.0021 0.0085 0.0005 0.0011 0.0013 0.0018 0.002 0.0007 0.0005
Bud Neck 0.0003 0.0004 0.0005 0.0007 0.001 0.0017 0.003 0.0003 0.0006 0.001 0.0017 0.0012 0.0024 0.0003 0.0019 0.0005 0.001 0.0016
Bud Periphery 0.0002 0.0012 0.0007 0.0005 0.0002 0.0003 0.0017 0.0005 0.0066 0.0029 0.0068 0.0003 0.0008 0.0005 0.0011 0.0014 0.0004 0.0003
Bud Site 0.0003 0.0105 0.0042 0.0003 0.0002 0.0002 0.0172 0.0027 0.0039 0.0153 0.0014 0.0002 0.0032 0.0024 0.0047 0.0016 0.0006 0.0004
Cell Periphery 0.0001 0.0004 0.0002 0 0 0.0001 0.0007 0.0002 0.0002 0.0004 0.0003 0 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001
Cytoplasm 0.8871 0.9301 0.9134 0.9237 0.934 0.8824 0.6982 0.8803 0.8551 0.7096 0.8192 0.9044 0.757 0.9302 0.8612 0.9384 0.9144 0.929
Cytoplasmic Foci 0.0035 0.0038 0.0056 0.0031 0.0028 0.0046 0.0273 0.0061 0.0185 0.0135 0.0497 0.0117 0.0202 0.0043 0.0132 0.0028 0.0167 0.0105
Eisosomes 0 0 0 0 0 0 0.0006 0 0.0001 0.0001 0 0 0.0002 0 0.0005 0 0 0
Endoplasmic Reticulum 0.0042 0.0032 0.0038 0.0032 0.0049 0.0051 0.0158 0.001 0.0025 0.0036 0.0043 0.002 0.0218 0.0029 0.003 0.0027 0.0054 0.0028
Endosome 0.0017 0.0029 0.0027 0.0025 0.0015 0.0044 0.0306 0.0054 0.005 0.0421 0.0235 0.0081 0.0363 0.0023 0.0087 0.0012 0.0146 0.0044
Golgi 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0.0095 0.0032 0.0024 0.0229 0.0027 0.0024 0.0079 0.0003 0.0017 0.0001 0.0009 0.0004
Lipid Particles 0.0001 0.0002 0.0002 0 0 0.0001 0.0192 0.0011 0.0046 0.0158 0.0085 0.0008 0.0085 0.0002 0.0007 0.0001 0.0006 0.0002
Mitochondria 0.0002 0.0006 0.0004 0.0003 0.0004 0.0005 0.0129 0.001 0.0028 0.0224 0.001 0.0007 0.0092 0.0002 0.0015 0.0002 0.0004 0.0003
None 0.0639 0.0115 0.0218 0.0036 0.0173 0.0406 0.0647 0.0735 0.0526 0.0801 0.0181 0.0484 0.0506 0.0425 0.0475 0.0305 0.0179 0.0211
Nuclear Periphery 0.0057 0.0041 0.0043 0.0058 0.0048 0.0059 0.0084 0.0037 0.0014 0.0011 0.0026 0.0014 0.0171 0.0018 0.0041 0.0021 0.0035 0.0026
Nucleolus 0.0001 0.0002 0.0003 0.0001 0 0.0002 0.0009 0 0.0001 0.0002 0.0009 0 0.0004 0.0001 0.0006 0.0001 0.0002 0.0001
Nucleus 0.0288 0.0216 0.032 0.0488 0.0286 0.0481 0.0102 0.0141 0.0054 0.0057 0.0094 0.0071 0.011 0.0054 0.0167 0.0131 0.0133 0.0146
Peroxisomes 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0196 0.001 0.0079 0.039 0.0179 0.0046 0.0119 0.0001 0.0021 0.0001 0.0004 0.0005
Punctate Nuclear 0.0006 0.0021 0.0028 0.0012 0.0011 0.0019 0.0082 0.0005 0.0022 0.0006 0.005 0.0014 0.0064 0.0012 0.0021 0.0009 0.004 0.0045
Vacuole 0.0024 0.0029 0.0028 0.0027 0.0015 0.0018 0.004 0.0006 0.0011 0.0029 0.0139 0.0007 0.0039 0.0017 0.0031 0.001 0.0015 0.0013
Vacuole Periphery 0.0002 0.0005 0.0003 0.0004 0.0003 0.0003 0.0017 0.0001 0.0002 0.0017 0.0012 0.0002 0.0019 0.0001 0.0008 0.0002 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.9091 5.4011 9.5186 21.0679 3.9168 7.5482 8.7035 10.8272 17.4361 6.4244
Translational Efficiency 0.7676 0.9807 1.4849 1.0769 1.1362 0.8272 0.5309 0.9179 0.8168 0.7509

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description L-homoserine-O-acetyltransferase; catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway
Localization
Cell Percentages cytoplasm (23%), nucleus (8%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Met2

Met2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available