Standard name | |
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Human Ortholog | |
Description | C-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 0 | 10 | 1 | 9 | 4 | 8 | – | 11 | 21 | 10 | – | – | – | – | – | – | 0 | 2 | 1 | 0 | 0 | 0 |
Bud | – | – | 0 | 0 | 0 | 1 | 3 | 2 | – | 4 | 6 | 3 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | 2 | 6 | 8 | 6 | 6 | 5 | – | 2 | 7 | 4 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | 4 | 8 | 15 | 12 | 16 | 22 | – | 14 | 21 | 11 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | – | 87 | 127 | 78 | 130 | 84 | 164 | – | 83 | 64 | 58 | – | – | – | – | – | – | 5 | 26 | 131 | 2 | 5 | 2 |
Endosome | – | – | 1 | 2 | 1 | 3 | 3 | 5 | – | 3 | 3 | 2 | – | – | – | – | – | – | 0 | 2 | 0 | 0 | 0 | 0 |
Golgi | – | – | 8 | 32 | 28 | 12 | 15 | 14 | – | 4 | 0 | 4 | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | – | – | 3 | 12 | 0 | 18 | 12 | 11 | – | 42 | 78 | 42 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | – | 1 | 2 | 0 | 1 | 0 | 1 | – | 0 | 4 | 1 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 58 | 77 | 56 | 98 | 52 | 96 | – | 76 | 115 | 82 | – | – | – | – | – | – | 2 | 3 | 14 | 0 | 0 | 0 |
Nucleolus | – | – | 1 | 1 | 1 | 3 | 1 | 1 | – | 5 | 13 | 3 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | – | – | 25 | 23 | 30 | 34 | 26 | 78 | – | 41 | 72 | 52 | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Unique Cell Count | 139 | 210 | 167 | 231 | 171 | 330 | 207 | 328 | 205 | 10 | 39 | 155 | 3 | 10 | 4 | |||||||||
Labelled Cell Count | 190 | 300 | 218 | 327 | 222 | 407 | 285 | 404 | 273 | 10 | 39 | 155 | 3 | 10 | 4 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 16.3 | 17.3 | 16.6 | 14.3 | 14.1 | 13.8 | – | 11.6 | 11.5 | 11.9 | – | – | – | – | – | – | – | – | – |
Std Deviation (1e-4) | – | – | 3.2 | 3.6 | 3.5 | 2.8 | 2.6 | 2.5 | – | 2.1 | 2.1 | 2.3 | – | – | – | – | – | – | – | – | – |
Intensity Change (Log2) | – | – | – | 0.09 | 0.03 | -0.19 | -0.21 | -0.24 | – | -0.49 | -0.5 | -0.45 | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.1 | 0 | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cytoplasm | 0 | 2.2 | 1.1 | 2.3 | 1.6 | – | 1.6 | 1.5 | 1.1 | – | – | – | – | – | – |
Endoplasmic Reticulum | -0.4 | -2.8 | -1.2 | -2.4 | -2.6 | – | -4.1 | -9.1 | -6.3 | – | – | – | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Golgi | 2.7 | 3.0 | -0.2 | 1.0 | -0.7 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Mitochondria | 1.6 | 0 | 2.3 | 2.0 | 0 | – | 4.9 | 5.6 | 4.9 | – | – | – | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Nuclear Periphery | -1.0 | -1.5 | 0.1 | -2.1 | -2.7 | – | -0.9 | -1.4 | -0.3 | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Vacuole | -1.9 | 0 | -0.8 | -0.7 | 1.3 | – | 0.4 | 1.0 | 1.6 | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.3092 | 0.0017 | 0.0567 | 0.0566 | 0.1267 | 0.0025 | 0.2368 | 0.0003 | 0.0075 | 0.0389 | 0 | 0.0036 | 0.3325 | 0.0033 | 0.031 | 0 | 0.0013 | 0.0002 |
Bud | 0.0027 | 0.0003 | 0.0013 | 0 | 0.0045 | 0.0004 | 0.0028 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0014 | 0.0004 | 0.0004 | 0 | 0.0126 | 0 |
Bud Neck | 0.0101 | 0.0005 | 0.0013 | 0.0004 | 0.0035 | 0.0009 | 0.0061 | 0.0001 | 0.0002 | 0.0011 | 0 | 0.0005 | 0.0012 | 0.0003 | 0.0005 | 0 | 0.001 | 0.0001 |
Bud Periphery | 0.0049 | 0.0004 | 0.0017 | 0.0001 | 0.0081 | 0.0005 | 0.0028 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0024 | 0.0003 | 0.0007 | 0 | 0.0154 | 0 |
Bud Site | 0.0114 | 0.0072 | 0.0279 | 0.0004 | 0.0362 | 0.0024 | 0.0388 | 0 | 0.0002 | 0.0025 | 0 | 0.0001 | 0.0081 | 0.0069 | 0.0015 | 0 | 0.0252 | 0 |
Cell Periphery | 0.0015 | 0.0097 | 0.0027 | 0.0212 | 0.0045 | 0.0009 | 0.001 | 0.012 | 0.0024 | 0.0012 | 0.0028 | 0.0015 | 0.0025 | 0.0118 | 0.0099 | 0.0015 | 0.0009 | 0.0006 |
Cytoplasm | 0.0097 | 0.0224 | 0.0246 | 0.0072 | 0.0079 | 0.0297 | 0.0227 | 0.0017 | 0.0023 | 0.0098 | 0.0007 | 0.0004 | 0.0074 | 0.0061 | 0.0018 | 0 | 0.017 | 0.0002 |
Cytoplasmic Foci | 0.038 | 0.007 | 0.0098 | 0.0067 | 0.0153 | 0.0091 | 0.055 | 0 | 0.002 | 0.0085 | 0 | 0.008 | 0.0098 | 0.0053 | 0.0014 | 0 | 0.0269 | 0 |
Eisosomes | 0.0016 | 0.0005 | 0.0002 | 0.0001 | 0.0015 | 0 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0044 | 0.0003 | 0.0004 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.2046 | 0.838 | 0.775 | 0.8525 | 0.5864 | 0.8517 | 0.2857 | 0.8594 | 0.7511 | 0.8735 | 0.9759 | 0.8878 | 0.4085 | 0.8504 | 0.7525 | 0.9855 | 0.7647 | 0.7462 |
Endosome | 0.0361 | 0.0027 | 0.002 | 0.0004 | 0.0374 | 0.0063 | 0.0726 | 0.0003 | 0.045 | 0.0222 | 0.0001 | 0.0052 | 0.0107 | 0.0017 | 0.0085 | 0 | 0.0368 | 0.0005 |
Golgi | 0.0191 | 0.0012 | 0.0033 | 0.001 | 0.0179 | 0.0035 | 0.0218 | 0.0005 | 0.0408 | 0.0115 | 0 | 0.0057 | 0.0082 | 0.0011 | 0.005 | 0 | 0.0047 | 0.0016 |
Lipid Particles | 0.0318 | 0.0076 | 0.011 | 0.0136 | 0.0294 | 0.0027 | 0.0378 | 0.0009 | 0.0081 | 0.0089 | 0.0034 | 0.012 | 0.0269 | 0.0141 | 0.026 | 0.0002 | 0.0049 | 0.0036 |
Mitochondria | 0.0459 | 0.0009 | 0.0013 | 0.0001 | 0.0172 | 0.0007 | 0.0075 | 0.0002 | 0.0078 | 0.0014 | 0 | 0.0006 | 0.0214 | 0.0018 | 0.012 | 0 | 0.0029 | 0.0001 |
None | 0.012 | 0.0011 | 0.0009 | 0.0001 | 0.0044 | 0.001 | 0.008 | 0.0001 | 0.0003 | 0.0009 | 0 | 0.0001 | 0.0152 | 0.0046 | 0.0008 | 0 | 0.007 | 0.0001 |
Nuclear Periphery | 0.1013 | 0.07 | 0.0261 | 0.0101 | 0.0365 | 0.0795 | 0.1397 | 0.1085 | 0.1153 | 0.0136 | 0.011 | 0.0573 | 0.113 | 0.0655 | 0.1199 | 0.0108 | 0.0372 | 0.2335 |
Nucleolus | 0.0119 | 0.0012 | 0.0131 | 0 | 0.0014 | 0.0001 | 0.0017 | 0 | 0.0001 | 0 | 0 | 0 | 0.0033 | 0.0013 | 0.0022 | 0 | 0.0032 | 0.0001 |
Nucleus | 0.0495 | 0.0056 | 0.0091 | 0.0004 | 0.0034 | 0.0022 | 0.0055 | 0.0017 | 0.0019 | 0.0005 | 0 | 0.0005 | 0.0054 | 0.0023 | 0.0064 | 0 | 0.0046 | 0.0037 |
Peroxisomes | 0.0398 | 0.0012 | 0.0148 | 0.0104 | 0.0142 | 0.001 | 0.0213 | 0 | 0.0001 | 0.0006 | 0 | 0.0107 | 0.0049 | 0.009 | 0.0005 | 0 | 0.004 | 0 |
Punctate Nuclear | 0.0396 | 0.0017 | 0.0096 | 0.0003 | 0.0108 | 0.0007 | 0.0214 | 0 | 0.0006 | 0.0008 | 0 | 0.0016 | 0.0042 | 0.0007 | 0.0019 | 0 | 0.0039 | 0.0002 |
Vacuole | 0.0103 | 0.0159 | 0.0068 | 0.0181 | 0.029 | 0.003 | 0.0047 | 0.0094 | 0.009 | 0.0025 | 0.0061 | 0.0031 | 0.0055 | 0.0078 | 0.0109 | 0.0016 | 0.0226 | 0.0016 |
Vacuole Periphery | 0.0087 | 0.0033 | 0.0006 | 0.0003 | 0.0035 | 0.0012 | 0.0054 | 0.0047 | 0.0049 | 0.0008 | 0.0001 | 0.0012 | 0.003 | 0.0048 | 0.0059 | 0.0003 | 0.0031 | 0.0076 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.8093 | 52.427 | 46.1279 | 57.1303 | 52.6839 | 52.7532 | 61.9068 | 65.6771 | 67.2319 | 71.8471 |
Translational Efficiency | 0.9951 | 0.8809 | 1.0719 | 0.8803 | 0.8444 | 1.0127 | 0.8073 | 0.6809 | 0.7972 | 0.815 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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