Standard name
Human Ortholog
Description Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 0.98 0.96 0.97 – 0.78 0.75 0.75 0.88 0.78 1.0 1.0 1.0 0.95 0.95 0.92 0.98 0.89 0.96 0.97 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.06 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0.06 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.11 0.16 0.12 0.05 0.09 0 0 0 0.06 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 6 6 3 1 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2 0 2 0 0 0 – 3 0 1 2 1 0 1 0 1 1 0 3 0 0 0 2 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 0 0 4 – 7 11 11 5 9 3 0 1 3 6 2 0 0 0 0 0 0
Cytoplasm 508 61 114 121 206 342 – 403 261 263 266 240 230 368 341 344 333 340 497 67 108 152 205 214
Endoplasmic Reticulum 3 0 0 1 4 9 – 31 11 23 8 15 0 1 0 5 6 14 1 0 0 0 0 2
Endosome 1 0 0 0 0 0 – 7 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 1 0 0 0 0 0 – 0 0 2 1 2 0 0 0 1 2 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 1 1 – 13 20 17 19 20 0 0 1 1 0 0 0 1 0 0 0 0
Nucleus 0 0 0 1 1 0 – 4 0 6 2 1 0 1 0 2 0 0 1 2 0 1 2 2
Nuclear Periphery 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 0 3 1 – 59 54 41 15 28 0 0 1 20 32 40 1 0 1 0 3 2
Unique Cell Count 513 61 114 123 214 352 517 346 351 304 308 230 368 342 363 352 371 509 76 113 157 218 232
Labelled Cell Count 519 62 116 123 215 357 538 365 370 321 321 233 371 344 378 380 398 509 76 113 157 218 232


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.6 32.9 19.4 24.0 25.5 23.3 – 20.8 20.0 19.7 18.6 18.6 31.9 33.0 31.4 40.2 40.4 38.6 29.4 32.1 34.4
Std Deviation (1e-4) 6.5 7.8 4.6 5.5 5.9 5.8 – 6.7 6.8 6.0 6.3 6.3 8.5 12.4 7.1 18.6 21.9 22.8 8.3 11.2 12.5
Intensity Change (Log2) – – – 0.31 0.39 0.26 – 0.09 0.04 0.02 -0.06 -0.07 0.72 0.77 0.69 1.05 1.05 0.99 0.6 0.72 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -2.1 -1.8 – -5.5 -5.9 -5.9 -4.0 -5.5 0 0 -0.6 -2.5 -2.5 -3.2
Endoplasmic Reticulum 0 0 0 – 2.7 0 2.8 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 2.7 2.8 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 3.8 4.5 3.8 0 3.3 0 0 0 0 3.3 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.222 27.9651 22.5308 22.0637 15.9937 24.59 21.9226 27.6586 26.0218 23.1441 25.0368 25.0885 26.3448 30.8131 30.113 28.5531 26.835 28.3077
Actin 0.0222 0.0026 0.0123 0.0396 0.0122 0.0005 0.0154 0.0017 0.0046 0.0015 0.0023 0.0016 0.0035 0.0001 0.0059 0.003 0.0059 0.0006
Bud 0.0003 0.0002 0.0006 0.0007 0.0025 0.0001 0.0011 0.0007 0.0008 0.0001 0.0011 0.0001 0.0008 0 0.0001 0.0009 0.0004 0.0001
Bud Neck 0.0005 0.0002 0.0006 0.0005 0.0007 0.0004 0.0013 0.0001 0.0004 0.0007 0.0003 0.0009 0.0003 0.0001 0.0002 0.0003 0.0003 0.0005
Bud Periphery 0.0003 0.0001 0.0005 0.001 0.003 0.0001 0.0011 0.0008 0.0004 0.0001 0.002 0.0001 0.0005 0 0 0.0008 0.0002 0.0001
Bud Site 0.0014 0.001 0.0048 0.0012 0.0045 0 0.0095 0.0039 0.0016 0.0004 0.0008 0.0001 0.0003 0 0.0001 0.0002 0.0005 0
Cell Periphery 0.0002 0 0.0001 0.0001 0.0004 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0003 0.0001
Cytoplasm 0.857 0.9797 0.9117 0.893 0.6346 0.947 0.8646 0.9819 0.9313 0.9049 0.9079 0.9576 0.9259 0.9824 0.9706 0.974 0.9109 0.9478
Cytoplasmic Foci 0.0176 0.0008 0.0118 0.0045 0.0135 0.0006 0.0235 0.0003 0.0223 0.0208 0.0018 0.0017 0.0062 0.0002 0.0014 0.0007 0.0145 0.0007
Eisosomes 0.0001 0 0.0001 0.0002 0.0002 0 0.0002 0 0 0 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0044 0.0001 0.0028 0.0015 0.0061 0.0005 0.0022 0.0002 0.0007 0.0006 0.0029 0.0057 0.0015 0.0001 0.0011 0.0006 0.0027 0.0008
Endosome 0.024 0.0014 0.0167 0.0139 0.1298 0.0042 0.0217 0.0003 0.0103 0.0041 0.0427 0.0024 0.0053 0 0.0024 0.0005 0.0038 0.0022
Golgi 0.0081 0.0031 0.0078 0.0096 0.0454 0.006 0.0066 0.0002 0.0048 0.0049 0.0088 0.0005 0.001 0 0.0007 0.0001 0.0009 0.0003
Lipid Particles 0.0107 0.0002 0.0025 0.0068 0.0088 0.0001 0.0052 0 0.0047 0.0047 0.0006 0.0004 0.0065 0 0.0012 0 0.0032 0.0005
Mitochondria 0.0038 0.0043 0.0097 0.0091 0.102 0.0268 0.005 0.0002 0.002 0.0275 0.0129 0.0006 0.0023 0 0.0002 0.0008 0.0031 0.0007
None 0.0036 0.0001 0.0006 0.001 0.0012 0.0003 0.0036 0.0002 0.0006 0.0007 0.0003 0.0004 0.0014 0.0002 0.0008 0.0005 0.0041 0.0004
Nuclear Periphery 0.0098 0.0006 0.0039 0.0045 0.0045 0.0013 0.0041 0.0011 0.0011 0.0022 0.0019 0.0057 0.0038 0.0014 0.0025 0.0015 0.0056 0.0071
Nucleolus 0.0003 0 0.0001 0.0002 0.0005 0 0.0014 0 0.0001 0.0002 0 0 0.0003 0 0 0 0.0041 0.0006
Nucleus 0.0274 0.0047 0.0066 0.0052 0.0066 0.011 0.02 0.008 0.0085 0.0184 0.0072 0.0206 0.0282 0.0152 0.0107 0.015 0.0198 0.0321
Peroxisomes 0.0016 0.0002 0.0036 0.0037 0.0026 0 0.0024 0 0.0039 0.0014 0.0001 0.0001 0.007 0 0.0005 0.0001 0.0008 0.0001
Punctate Nuclear 0.0039 0.0001 0.0008 0.0024 0.0006 0.0002 0.0043 0.0001 0.0011 0.0061 0.0001 0.0009 0.0013 0.0001 0.0006 0.0001 0.0053 0.0004
Vacuole 0.0019 0.0002 0.0012 0.0005 0.012 0.0004 0.0052 0.0002 0.0006 0.0003 0.004 0.0005 0.0029 0.0001 0.0007 0.0004 0.0072 0.0038
Vacuole Periphery 0.0008 0.0001 0.0011 0.0007 0.0084 0.0005 0.0013 0 0.0003 0.0003 0.0019 0.0002 0.0008 0 0.0002 0.0001 0.0063 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 169.5708 207.0963 205.9554 201.5881 182.6972 171.8639 236.3787 226.561 267.7078 187.7961
Translational Efficiency 1.2729 1.4615 1.4386 1.7348 1.4849 1.586 1.4996 1.8768 1.549 1.6409

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Hch1

Hch1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hch1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available