Standard name
Human Ortholog
Description Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.48 0.33 0.37 0.34 0.22 0.28 0.24 0.23 – 0.14 0.15 0.13 0.26 0.21 0.16 0.22 0.28 0.28 0.11 0.16 0.12 0.19 0.13 0.08
Bud 0.11 0.12 0.09 0.15 0.11 0.17 0.14 0.16 – 0.13 0.18 0.12 0.16 0.09 0 0.06 0.06 0 0 0 0 0.1 0.07 0.05
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.17 0.09 0.14 0.07 0.06
Bud Site 0.05 0 0 0.1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.41 0.42 0.37 0.18 0.17 0.16 0.2 0.1 – 0.1 0.14 0.18 0.63 0.66 0.67 0.56 0.46 0.43 0.61 0.44 0.65 0.47 0.65 0.71
Cytoplasm 0.23 0.4 0.34 0.37 0.47 0.58 0.52 0.61 – 0.68 0.61 0.69 0.39 0.31 0.38 0.32 0.36 0.35 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.07 0 0 – 0 0 0 0 0 0 0.09 0.05 0 0.09 0.06 0.06 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.08 0.08 0.16 0.17 0.16 – 0.18 0.24 0.2 0 0 0 0.13 0.06 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.12 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.08 0 0.1 0.08 0.1 0 0 0 – 0 0 0 0.08 0 0 0 0 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 105 122 113 56 39 78 86 80 – 41 59 50 16 15 21 71 95 100 35 81 43 26 24 17
Bud 25 46 27 25 19 46 49 56 – 40 71 46 10 6 6 18 21 14 3 13 4 13 13 10
Bud Neck 0 1 11 4 2 5 3 3 – 0 3 1 0 0 0 1 2 0 21 85 33 18 12 12
Bud Site 12 14 12 16 4 7 9 8 – 6 2 10 0 0 1 1 1 3 – – – – – –
Cell Periphery 90 155 112 30 31 45 71 34 – 30 55 69 39 46 90 183 153 151 190 216 243 65 118 151
Cytoplasm 51 147 102 62 84 160 186 212 – 205 236 267 24 22 51 104 119 122 3 22 2 0 0 3
Endoplasmic Reticulum 7 13 5 3 4 18 15 10 – 9 9 10 1 1 4 29 16 13 28 28 21 7 4 8
Endosome 9 0 2 3 4 1 0 3 – 0 0 0 0 0 0 3 2 1 3 4 0 0 0 0
Golgi 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 3 0 0 0
Mitochondria 7 9 17 14 15 45 61 56 – 54 93 79 0 0 1 42 19 20 7 12 5 2 3 3
Nucleus 2 1 0 1 1 0 2 1 – 1 0 1 0 0 1 3 2 8 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 2 0 1 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 7 0 8 7 21 5 6 5 – 3 0 3 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 18 3 30 14 18 1 8 7 – 4 4 7 5 3 2 10 15 29 0 0 0 0 0 0
Vac/Vac Membrane 1 0 3 0 3 0 0 1 – 0 3 1 0 0 0 3 3 2 12 24 7 1 2 1
Unique Cell Count 219 369 304 166 179 276 360 350 301 388 389 62 70 135 327 334 351 312 495 373 138 184 215
Labelled Cell Count 339 512 443 235 245 411 496 476 393 537 544 95 93 177 468 448 463 312 495 373 138 184 215


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.6 5.8 5.1 5.8 4.7 4.7 5.0 – 4.4 4.3 4.5 7.9 8.1 8.6 7.6 8.4 9.1 9.3 10.4 11.7
Std Deviation (1e-4) 1.2 1.2 1.3 1.3 2.1 1.3 1.0 0.9 – 1.0 1.0 0.8 1.4 1.5 1.9 1.2 1.5 1.6 1.7 1.9 2.2
Intensity Change (Log2) – – – -0.19 -0.0 -0.31 -0.3 -0.2 – -0.41 -0.42 -0.35 0.45 0.48 0.57 0.4 0.54 0.65 0.68 0.84 1.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.7 -3.5 0 0 -4.0 – 0 0 0 -1.7 -2.5 -4.5 -4.3 -2.3 -2.4
Bud 2.0 0.6 0 0 2.7 – 0 0 0 1.7 -0.1 -1.6 -1.6 -1.2 -2.6
Bud Neck -0.7 0 0 0 -2.4 – 0 0 0 0 0 0 -3.0 -2.7 -3.6
Bud Site 2.5 -1.0 0 0 -1.2 – 0 0 0 0 0 0 -3.2 -3.3 -2.6
Cell Periphery -4.2 -4.5 0 0 -8.3 – 0 0 0 3.8 4.4 5.8 4.8 2.3 1.6
Cytoplasm 0.8 2.9 5.9 4.7 6.9 – 8.5 7.1 9.2 0.8 -0.3 0.9 -0.5 0.6 0.3
Endoplasmic Reticulum 0 0 0 0 1.0 – 0 0 0 0 0 0 4.0 2.2 1.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 1.2 0 0 4.2 – 0 0 0 0 0 -2.4 3.1 0.1 0.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0.9 4.1 0 0 -1.1 – 0 0 0 0 0 0 0 0 0
SpindlePole -0.5 0.1 0 0 -4.3 – 0 0 0 -0.4 -1.5 -3.1 -3.5 -2.6 -0.7
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4425 5.1796 5.2677 4.8286 4.6272 4.7219 5.8874 6.4472 6.5189 6.5125 6.5872 6.568 5.9103 5.783 5.8827 6.2217 6.19 6.315
Actin 0.0107 0.0157 0.0182 0.0035 0.0044 0.0041 0.0125 0.0098 0.0087 0.0033 0.0035 0.0045 0.0135 0.0235 0.0235 0.0041 0.0282 0.0068
Bud 0.0072 0.0089 0.0199 0.0094 0.005 0.0034 0.0031 0.0034 0.0101 0.0037 0.0012 0.0025 0.0018 0.0027 0.0141 0.0039 0.0018 0.002
Bud Neck 0.0074 0.0078 0.0274 0.0315 0.01 0.077 0.0083 0.0105 0.0504 0.0751 0.058 0.0903 0.0041 0.0125 0.0299 0.0496 0.0158 0.0792
Bud Periphery 0.0666 0.0406 0.1619 0.1673 0.1013 0.051 0.042 0.0194 0.1129 0.1051 0.0298 0.0339 0.025 0.0185 0.1283 0.0877 0.0445 0.0354
Bud Site 0.0329 0.2329 0.0979 0.0174 0.0084 0.0114 0.0428 0.3332 0.1149 0.0135 0.0189 0.0099 0.0267 0.2981 0.0996 0.0105 0.0028 0.0069
Cell Periphery 0.547 0.2831 0.4272 0.4315 0.581 0.5226 0.6774 0.4391 0.5538 0.6458 0.731 0.6666 0.7287 0.387 0.5625 0.6865 0.6872 0.6627
Cytoplasm 0.2208 0.2863 0.163 0.2151 0.185 0.2068 0.1682 0.1517 0.1204 0.1202 0.137 0.1671 0.1543 0.2243 0.1188 0.1375 0.1942 0.1846
Cytoplasmic Foci 0.0028 0.0044 0.0031 0.0047 0.0012 0.0026 0.0008 0.0006 0.0009 0.0003 0.0005 0.0004 0.0013 0.0005 0.0005 0.0003 0.0005 0.0003
Eisosomes 0.0049 0.0039 0.0043 0.0007 0.0017 0.0011 0.0095 0.0038 0.0021 0.0022 0.0013 0.0014 0.0036 0.0064 0.0039 0.0005 0.0051 0.0011
Endoplasmic Reticulum 0.0127 0.01 0.0061 0.0116 0.0072 0.0056 0.0068 0.0068 0.004 0.0164 0.005 0.0045 0.0075 0.0043 0.0067 0.0048 0.0034 0.0024
Endosome 0.0124 0.0129 0.009 0.0131 0.0036 0.0067 0.0003 0.0002 0.0006 0.0001 0.0001 0.0001 0.0006 0.0002 0.0003 0.0002 0.0001 0.0001
Golgi 0.0012 0.0019 0.0009 0.0011 0.0004 0.0005 0.0006 0.0003 0.0006 0.0004 0.0003 0.0002 0.001 0.0005 0.0005 0.0002 0.0006 0.0002
Lipid Particles 0.003 0.003 0.0008 0.0011 0.0013 0.0023 0.0023 0.0012 0.0013 0.0029 0.0008 0.0012 0.0015 0.0009 0.0007 0.0004 0.0007 0.0005
Mitochondria 0.0008 0.0052 0.0008 0.0071 0.0003 0.0004 0.0002 0.0001 0.0006 0.0001 0.0002 0.0001 0.0038 0.0001 0.0001 0.0001 0.0001 0
None 0.0265 0.0261 0.0155 0.0394 0.0265 0.0187 0.0156 0.0123 0.0082 0.0006 0.0064 0.0092 0.0185 0.0139 0.0014 0.0008 0.0111 0.0104
Nuclear Periphery 0.0008 0.0014 0.0007 0.001 0.0005 0.0005 0.0005 0.0005 0.0004 0.0007 0.0002 0.0002 0.0005 0.0005 0.0004 0.0003 0.0002 0.0001
Nucleolus 0.0005 0.0013 0.0006 0.0008 0.0007 0.0008 0.0002 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0008 0.0032 0.0016 0.0026 0.0022 0.0021 0.0004 0.0007 0.0007 0.0005 0.0005 0.0006 0.0002 0.0005 0.0006 0.0005 0.0002 0.0004
Peroxisomes 0.0002 0.0004 0.0001 0.0002 0 0.0001 0 0.0001 0.0012 0 0 0 0.0008 0 0.0003 0 0 0
Punctate Nuclear 0.0001 0.0007 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0 0 0
Vacuole 0.0392 0.0489 0.0402 0.0379 0.0585 0.08 0.0082 0.0061 0.0076 0.0084 0.0049 0.0068 0.0063 0.0053 0.0073 0.0116 0.0035 0.0066
Vacuole Periphery 0.0015 0.0015 0.0007 0.0029 0.0006 0.0018 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.045 71.1078 28.0768 26.2907 62.9204 51.7846 60.8486 44.7415 28.6996 77.6347
Translational Efficiency 0.5141 0.4177 0.2882 0.3556 0.5917 0.8027 0.3783 0.3608 0.4015 0.39

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (67%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Wsc2

Wsc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wsc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available