Standard name | |
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Human Ortholog | |
Description | Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; regulates toggling of the U2 snRNA stem II region between different structures; contains two RNA recognition motifs (RRMs) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 |
Bud | 0 | 0 | 0 | 1 | 3 | 1 | 10 | 1 | 7 | 5 | 6 | 6 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 5 | 1 | 1 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 2 | 17 | 3 | 17 | 7 | 11 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 2 | 19 | 24 | 32 | 24 | 48 | 40 | 31 | 26 | 19 | 21 | 8 | 26 | 27 | 45 | 29 | 30 | 4 | 1 | 3 | 7 | 7 | 8 |
Endoplasmic Reticulum | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 15 | 10 | 8 | 0 | 0 | 1 | 2 | 1 | 1 |
Endosome | 1 | 0 | 3 | 1 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 9 | 3 | 2 | 3 | 1 | 2 | 3 | 3 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 1 | 1 | 0 |
Mitochondria | 12 | 24 | 4 | 14 | 57 | 57 | 206 | 63 | 245 | 183 | 158 | 175 | 2 | 1 | 0 | 18 | 4 | 3 | 2 | 4 | 3 | 1 | 2 | 8 |
Nucleus | 266 | 152 | 386 | 261 | 297 | 254 | 255 | 406 | 361 | 466 | 271 | 324 | 61 | 123 | 145 | 238 | 159 | 181 | 271 | 153 | 373 | 86 | 184 | 131 |
Nuclear Periphery | 0 | 0 | 0 | 2 | 0 | 4 | 1 | 3 | 0 | 6 | 1 | 6 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 7 | 11 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 15 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 2 | 1 | 2 | 4 | 6 | 2 | 10 | 3 | 6 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 6 | 3 | 3 | 4 |
Vac/Vac Membrane | 11 | 6 | 7 | 8 | 10 | 16 | 2 | 8 | 2 | 12 | 5 | 14 | 2 | 4 | 6 | 35 | 23 | 17 | 18 | 7 | 31 | 12 | 26 | 45 |
Unique Cell Count | 294 | 165 | 417 | 297 | 351 | 316 | 375 | 486 | 466 | 588 | 343 | 421 | 70 | 155 | 178 | 319 | 211 | 226 | 314 | 174 | 442 | 132 | 250 | 226 |
Labelled Cell Count | 304 | 185 | 424 | 313 | 403 | 365 | 548 | 536 | 667 | 727 | 485 | 570 | 73 | 157 | 181 | 366 | 230 | 247 | 314 | 174 | 442 | 132 | 250 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 4.5 | 6.3 | 5.9 | 5.1 | 5.1 | 3.4 | 5.3 | 4.2 | 4.6 | 4.2 | 4.5 | 6.2 | 6.3 | 5.8 | 5.9 | 7.3 | 7.1 | 7.0 | 7.2 | 7.3 |
Std Deviation (1e-4) | 0.9 | 0.8 | 1.5 | 1.6 | 2.0 | 1.7 | 0.9 | 1.4 | 1.3 | 0.9 | 1.0 | 1.1 | 1.4 | 1.6 | 1.4 | 1.4 | 2.1 | 1.8 | 2.4 | 2.1 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.3 | -0.29 | -0.86 | -0.24 | -0.59 | -0.46 | -0.57 | -0.48 | -0.02 | 0.01 | -0.12 | -0.09 | 0.23 | 0.18 | 0.16 | 0.2 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.4 | 5.1 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7134 | 2.4901 | 2.1476 | 1.8155 | 1.7516 | 2.0702 | 4.3879 | 5.3573 | 4.7011 | 4.4922 | 4.2854 | 4.855 | 2.2213 | 2.614 | 2.4334 | 2.8474 | 2.1644 | 2.6838 |
Actin | 0.0215 | 0 | 0.0055 | 0.0006 | 0.001 | 0.0001 | 0.0044 | 0 | 0.0148 | 0.0001 | 0.0036 | 0.0001 | 0.0138 | 0.0009 | 0.0005 | 0 | 0.0049 | 0.0018 |
Bud | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Bud Neck | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0554 | 0.0022 | 0.0082 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0015 | 0.0051 | 0.0001 | 0.0004 | 0.0001 | 0.0022 | 0.0013 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0019 | 0 | 0.0007 | 0 | 0 | 0 | 0.0009 | 0.0001 | 0.0003 | 0 | 0.0006 | 0.0004 |
Bud Site | 0.0013 | 0.0008 | 0.0004 | 0 | 0.0021 | 0.0001 | 0.0009 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0031 | 0.0003 | 0.0003 | 0 | 0.0005 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0041 | 0.0048 | 0.0074 | 0.0136 | 0.0036 | 0.0028 | 0.0065 | 0.0037 | 0.009 | 0.0056 | 0.0058 | 0.015 | 0.0089 | 0.0091 | 0.0047 | 0.0019 | 0.0134 | 0.0016 |
Cytoplasmic Foci | 0.0185 | 0.0001 | 0.0064 | 0.0002 | 0.0055 | 0.0001 | 0.0134 | 0 | 0.0006 | 0.0001 | 0.0025 | 0.0009 | 0.007 | 0.0017 | 0.0003 | 0 | 0.0061 | 0.0001 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0011 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0017 | 0.0005 | 0.0015 | 0.0012 | 0.0005 | 0.0001 | 0.0021 | 0.0003 | 0.0032 | 0.0001 | 0.0004 | 0.0003 | 0.0014 | 0.0006 | 0.0008 | 0.0001 | 0.0032 | 0.0006 |
Endosome | 0.0178 | 0.0003 | 0.0045 | 0.002 | 0.0046 | 0.0002 | 0.007 | 0.0002 | 0.0054 | 0.0001 | 0.0007 | 0.0005 | 0.0105 | 0.0018 | 0.0015 | 0.0001 | 0.0279 | 0.001 |
Golgi | 0.0041 | 0 | 0.0023 | 0 | 0.0018 | 0 | 0.0011 | 0 | 0.0051 | 0 | 0.0001 | 0 | 0.0012 | 0.0004 | 0.0002 | 0 | 0.004 | 0.0005 |
Lipid Particles | 0.0131 | 0.0001 | 0.0034 | 0.0002 | 0.0014 | 0 | 0.0058 | 0 | 0.0013 | 0 | 0.0013 | 0.0001 | 0.0028 | 0.0018 | 0.0015 | 0 | 0.0148 | 0.0002 |
Mitochondria | 0.0348 | 0.0002 | 0.0102 | 0.0011 | 0.0002 | 0.0001 | 0.0029 | 0.0001 | 0.0043 | 0.0001 | 0.0004 | 0.0002 | 0.0114 | 0.0007 | 0.0028 | 0.0001 | 0.0095 | 0.0018 |
None | 0.0029 | 0.0204 | 0.0181 | 0.0661 | 0.0005 | 0.0103 | 0.0044 | 0.0018 | 0.062 | 0.0763 | 0.1332 | 0.0721 | 0.0382 | 0.0367 | 0.0396 | 0.0005 | 0.0096 | 0.0028 |
Nuclear Periphery | 0.0116 | 0.0032 | 0.0168 | 0.0364 | 0.004 | 0.0021 | 0.0231 | 0.0028 | 0.0145 | 0.0056 | 0.0086 | 0.0056 | 0.0098 | 0.0185 | 0.0096 | 0.0043 | 0.0387 | 0.0057 |
Nucleolus | 0.005 | 0.0061 | 0.0034 | 0.0028 | 0.0121 | 0.0145 | 0.0081 | 0.0043 | 0.0059 | 0.0042 | 0.0072 | 0.0111 | 0.0062 | 0.012 | 0.005 | 0.0024 | 0.0161 | 0.0136 |
Nucleus | 0.8325 | 0.96 | 0.9045 | 0.8656 | 0.8791 | 0.9554 | 0.8683 | 0.9837 | 0.8592 | 0.901 | 0.8274 | 0.8654 | 0.8494 | 0.8889 | 0.9209 | 0.9876 | 0.807 | 0.9551 |
Peroxisomes | 0.015 | 0 | 0.0052 | 0.0001 | 0.0005 | 0 | 0.0011 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0113 | 0.0051 | 0.0001 | 0 | 0.0058 | 0 |
Punctate Nuclear | 0.0118 | 0.0014 | 0.0092 | 0.0071 | 0.0263 | 0.0115 | 0.0351 | 0.0022 | 0.0098 | 0.0058 | 0.0059 | 0.0265 | 0.0164 | 0.0199 | 0.0095 | 0.0027 | 0.0276 | 0.0123 |
Vacuole | 0.0017 | 0.0014 | 0.0006 | 0.0015 | 0.0009 | 0.0002 | 0.003 | 0.0006 | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0013 | 0.0007 | 0.0012 | 0.0001 | 0.0046 | 0.0004 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0 | 0.0018 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0005 | 0.0006 | 0 | 0.0033 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.7161 | 9.197 | 5.9715 | 10.745 | 11.6717 | 11.5678 | 9.8802 | 10.1989 | 12.6951 | 9.7087 |
Translational Efficiency | 1.3188 | 1.5497 | 2.1037 | 1.4282 | 1.2039 | 0.9427 | 1.2491 | 1.3851 | 1.1587 | 1.2023 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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