Standard name
Human Ortholog
Description Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.11 0 0 0 0 0.05 0
Bud 0 0 0.06 0.05 0.08 0.05 0.06 0.06 0.07 – 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0.08 0.29 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.11 0 0 0 0 0 0
Endosome 0.23 0.05 0.15 0.09 0.3 0.07 0.07 0.09 0 – 0 0 0.1 0.16 0.08 0.51 0.21 0.28 0.22 0.25 0.29 0.27 0.22 0.2
Golgi 0.51 0.18 0.1 0 0 0 0 0 0 – 0 0 0.38 0.36 0.09 0.26 0.31 0.33 0.66 0.54 0.46 0.53 0.6 0.61
Mitochondria 0.44 0.82 0.87 0.86 0.69 0.89 0.87 0.82 0.93 – 0.9 0.92 0.8 0.68 0.83 0 0.1 0.11 0 0.08 0.06 0.09 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.05 0.33 0.09 0.11 0.13 0.06 – 0.07 0.07 0 0 0 0 0 0 0.06 0 0.14 0 0 0.09
SpindlePole 0 0 0 0 0.09 0 0.07 0.08 0.07 – 0.06 0 0 0 0 0.13 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.07 0.05 0 0 0 0 0 0 – 0 0 0 0.07 0 0.13 0.14 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 11 8 1 0 3 2 2 3 – 3 2 2 6 5 2 2 2 8 10 5 4 11 9
Bud 6 6 20 16 40 27 50 56 56 – 52 68 6 4 12 1 1 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 9 10 25 – 15 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 1 1 1 0 2 8 0 – 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 15 2 16 18 11 27 25 13 – 12 10 6 10 11 3 12 3 0 0 0 1 0 0
Endoplasmic Reticulum 2 12 6 8 2 5 7 8 3 – 1 8 4 12 6 1 0 2 0 1 1 2 2 0
Endosome 43 15 51 31 160 36 54 92 14 – 12 16 18 39 22 20 9 5 47 80 108 38 55 45
Golgi 94 53 34 6 3 1 0 0 0 – 0 0 69 89 24 10 13 6 144 176 175 76 148 140
Mitochondria 80 234 304 299 363 485 713 833 742 – 493 704 147 168 225 0 4 2 1 26 24 12 15 6
Nucleus 0 1 1 1 0 1 3 1 2 – 2 3 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 2 4 3 2 2 7 11 6 9 – 4 15 4 2 8 1 2 0 0 0 1 0 0 0
Nucleolus 5 2 3 3 4 11 13 28 24 – 21 26 0 1 5 2 1 0 0 0 0 0 0 0
Peroxisomes 0 6 26 16 171 47 92 134 49 – 37 54 0 4 2 0 1 0 12 12 54 4 11 21
SpindlePole 5 2 5 4 47 19 59 83 54 – 32 34 2 0 3 5 2 0 2 1 1 0 0 1
Vac/Vac Membrane 15 19 17 5 8 10 14 20 9 – 8 4 7 18 11 5 6 2 1 9 4 1 0 1
Unique Cell Count 183 287 349 347 526 542 821 1016 797 548 768 183 248 270 39 42 18 221 325 380 144 249 230
Labelled Cell Count 267 382 481 409 819 665 1056 1306 1003 693 964 265 353 334 50 53 22 221 325 380 144 249 230


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 7.4 6.0 6.0 6.1 5.5 5.2 5.3 4.7 – 4.7 4.5 7.4 7.6 6.6 8.5 10.2 9.7 8.0 7.8 7.8
Std Deviation (1e-4) 1.3 1.6 0.8 1.2 1.2 1.1 1.4 1.3 1.0 – 1.4 1.4 1.1 1.2 1.0 2.1 2.7 1.4 1.3 1.2 1.3
Intensity Change (Log2) – – – 0.01 0.03 -0.11 -0.21 -0.19 -0.35 – -0.36 -0.41 0.31 0.35 0.13 0.51 0.77 0.7 0.42 0.39 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0.1 -0.4 0 0 0
Bud -0.7 1.1 -0.5 0.2 -0.2 0 – 0 0 -1.2 -2.5 -0.7 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.4 2.8 1.8 2.7 2.2 0 – 1.9 0 0 0 3.0 0 0 0
Endoplasmic Reticulum 0.6 0 0 0 0 0 – 0 0 0 2.2 0.5 0 0 0
Endosome -2.3 5.4 -3.9 -4.4 -2.9 0 – 0 0 -1.6 0.4 -2.5 5.6 1.2 0
Golgi -4.5 -6.6 -7.2 -9.1 -10.1 0 – 0 0 7.8 7.8 -0.4 0 4.0 0
Mitochondria -0.4 -6.2 1.1 -0.1 -2.2 0 – 0 0 -2.1 -5.7 -1.3 -12.5 -11.6 -8.4
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.4 0 2.1 0 – 0 0 0 0 0 0 0 0
Peroxisomes -1.6 8.7 0.6 2.0 2.9 0 – 0 0 -3.8 -3.2 -4.0 0 0 0
SpindlePole 0 4.6 1.9 4.0 4.4 0 – 0 0 0 0 0 0 0 0
Vacuole -2.6 -2.9 -2.6 -3.1 -2.9 -3.9 – -3.0 -5.0 -0.6 1.2 -0.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4916 8.9738 7.9816 7.1926 7.3944 8.5176 5.1094 7.5786 6.4886 5.9745 5.3652 6.7797 6.9995 6.8698 6.8273 7.1793 6.3333 7.1517
Actin 0.1976 0.0271 0.0179 0.0525 0.0606 0.0185 0.0492 0.0127 0.0224 0.0318 0.0421 0.012 0.0398 0.042 0.0083 0.0013 0.0049 0.0162
Bud 0.001 0.0002 0.0002 0.0004 0.0002 0.0003 0.0003 0.0042 0.0001 0.0005 0.0004 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001
Bud Neck 0.004 0.0004 0.0004 0.0009 0.0005 0.001 0.0005 0.0004 0.0003 0.0005 0.0004 0.0004 0.0003 0.0002 0.0002 0.0002 0.0003 0.0003
Bud Periphery 0.0016 0.0002 0.0002 0.0008 0.0003 0.0006 0.0003 0.0015 0.0001 0.0006 0.0006 0.0001 0.0003 0.0001 0.0003 0 0.0001 0.0001
Bud Site 0.0086 0.0004 0.0005 0.0026 0.0004 0.0005 0.0009 0.0043 0.0004 0.0014 0.001 0.0001 0.0005 0.0002 0.0016 0.0001 0.0002 0.0001
Cell Periphery 0.0006 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Cytoplasm 0.0079 0.0008 0.001 0.0007 0.015 0.0009 0.0057 0.0019 0.0008 0.0008 0.0007 0.0008 0.0022 0.0006 0.0006 0.0007 0.0011 0.0005
Cytoplasmic Foci 0.0657 0.1071 0.0938 0.0941 0.0784 0.0489 0.1087 0.1078 0.0758 0.0711 0.0671 0.0589 0.0926 0.1233 0.1039 0.079 0.1046 0.0648
Eisosomes 0.0017 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0087 0.0003 0.0011 0.0012 0.0019 0.0084 0.0038 0.0003 0.0007 0.0007 0.001 0.0005 0.005 0.0002 0.0002 0.0004 0.0008 0.0004
Endosome 0.1405 0.1622 0.1768 0.1833 0.1924 0.1816 0.193 0.1944 0.1653 0.2287 0.248 0.1645 0.1653 0.1409 0.1285 0.2472 0.22 0.1315
Golgi 0.3536 0.5638 0.5689 0.5525 0.3712 0.5714 0.4273 0.5893 0.6078 0.5791 0.4632 0.6842 0.5009 0.5872 0.6527 0.6031 0.5686 0.6719
Lipid Particles 0.0962 0.0762 0.0696 0.0536 0.1381 0.0802 0.1129 0.0171 0.0285 0.0225 0.0563 0.0399 0.0763 0.0457 0.0298 0.0536 0.0261 0.0331
Mitochondria 0.0249 0.0253 0.0318 0.0306 0.014 0.0398 0.0215 0.0211 0.031 0.0123 0.0262 0.0132 0.0256 0.032 0.0208 0.0027 0.0095 0.0123
None 0.0062 0 0.0004 0.0002 0.0013 0.0004 0.0091 0.0126 0.0003 0.0002 0.0003 0.0002 0.0021 0 0.0002 0 0.0004 0
Nuclear Periphery 0.017 0.0001 0.0007 0.0008 0.0135 0.0153 0.0031 0.0001 0.0017 0.0004 0.0075 0.0003 0.011 0.0001 0.0001 0.0002 0.0002 0.0001
Nucleolus 0.0008 0 0.0001 0.0002 0.0003 0.0001 0.0007 0.0002 0.0096 0.0001 0.0003 0.0005 0.0002 0 0.0003 0 0.0001 0
Nucleus 0.0149 0 0.0001 0.0002 0.0477 0.0007 0.0012 0.0001 0.0008 0.0001 0.0005 0.0001 0.0012 0 0.0001 0 0 0
Peroxisomes 0.0309 0.0337 0.0282 0.021 0.053 0.0193 0.0496 0.0289 0.0465 0.0446 0.0697 0.0179 0.0688 0.0261 0.0496 0.0069 0.048 0.0667
Punctate Nuclear 0.0125 0.0002 0.0007 0.0008 0.0027 0.0009 0.0042 0.0002 0.0045 0.0008 0.0009 0.0002 0.0027 0.0001 0.0007 0.0001 0.0118 0.0001
Vacuole 0.0023 0.0006 0.0029 0.0012 0.0026 0.0036 0.0044 0.0011 0.0012 0.0013 0.0033 0.0019 0.0021 0.0004 0.0006 0.0014 0.0013 0.0005
Vacuole Periphery 0.0029 0.0012 0.0044 0.0022 0.0057 0.0071 0.0032 0.0015 0.0021 0.0022 0.0101 0.004 0.0026 0.0007 0.001 0.0031 0.0019 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.5012 18.8799 23.5483 33.0235 25.6351 19.3118 28.1265 27.5501 29.0838 34.7484
Translational Efficiency 0.4242 0.5253 0.4839 0.3968 0.4223 0.5717 0.3875 0.3331 0.4484 0.3623

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins
Localization
Cell Percentages Golgi (16%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mon2

Mon2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mon2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available