Standard name | |
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Human Ortholog | |
Description | Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 23 | 90 | 57 | 78 | 72 | 42 | 47 | 23 | 23 | 28 | 23 | 41 | 30 | 50 | 33 | 31 | 35 | 28 | 18 | 45 | 26 | 25 | 47 | 44 |
Bud | 25 | 91 | 58 | 62 | 58 | 60 | 64 | 53 | 57 | 62 | 56 | 75 | 17 | 28 | 30 | 23 | 18 | 12 | 54 | 172 | 110 | 63 | 108 | 96 |
Bud Neck | 0 | 5 | 7 | 5 | 6 | 3 | 8 | 8 | 5 | 9 | 9 | 8 | 0 | 0 | 0 | 1 | 10 | 0 | 31 | 111 | 76 | 38 | 38 | 50 |
Bud Site | 17 | 49 | 29 | 42 | 18 | 30 | 27 | 7 | 19 | 21 | 16 | 25 | 15 | 6 | 0 | 9 | 7 | 4 | – | – | – | – | – | – |
Cell Periphery | 7 | 11 | 21 | 8 | 12 | 11 | 10 | 8 | 15 | 6 | 24 | 14 | 28 | 86 | 92 | 23 | 36 | 16 | 2 | 4 | 6 | 1 | 7 | 11 |
Cytoplasm | 93 | 266 | 210 | 126 | 198 | 191 | 237 | 214 | 265 | 211 | 244 | 278 | 160 | 331 | 324 | 160 | 157 | 137 | 58 | 134 | 123 | 41 | 110 | 188 |
Endoplasmic Reticulum | 1 | 7 | 3 | 8 | 3 | 3 | 5 | 0 | 2 | 3 | 2 | 3 | 5 | 3 | 6 | 13 | 17 | 11 | 1 | 5 | 3 | 4 | 18 | 12 |
Endosome | 8 | 9 | 1 | 5 | 4 | 7 | 5 | 1 | 1 | 1 | 3 | 0 | 11 | 5 | 7 | 8 | 4 | 5 | 0 | 14 | 5 | 2 | 6 | 5 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 1 |
Mitochondria | 4 | 20 | 2 | 3 | 6 | 15 | 30 | 20 | 78 | 99 | 107 | 69 | 1 | 1 | 0 | 8 | 3 | 7 | 2 | 10 | 10 | 5 | 5 | 11 |
Nucleus | 2 | 7 | 7 | 5 | 9 | 13 | 14 | 4 | 6 | 9 | 4 | 13 | 3 | 0 | 0 | 7 | 3 | 3 | 1 | 0 | 1 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 |
Nucleolus | 1 | 4 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 9 | 1 | 11 | 0 | 6 | 5 | 3 | 2 | 4 | 2 | 0 | 10 | 10 | 1 | 4 | 5 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
SpindlePole | 13 | 41 | 47 | 58 | 63 | 30 | 43 | 37 | 26 | 23 | 10 | 30 | 11 | 4 | 2 | 23 | 19 | 12 | 1 | 17 | 10 | 2 | 1 | 2 |
Vac/Vac Membrane | 1 | 18 | 19 | 15 | 18 | 18 | 7 | 14 | 4 | 9 | 9 | 4 | 1 | 0 | 2 | 18 | 22 | 10 | 1 | 22 | 11 | 9 | 11 | 11 |
Unique Cell Count | 158 | 467 | 351 | 289 | 351 | 337 | 387 | 322 | 394 | 354 | 375 | 440 | 226 | 413 | 390 | 252 | 232 | 196 | 179 | 552 | 396 | 198 | 369 | 444 |
Labelled Cell Count | 197 | 629 | 463 | 427 | 468 | 430 | 503 | 393 | 503 | 486 | 513 | 562 | 292 | 524 | 499 | 331 | 339 | 246 | 179 | 552 | 396 | 198 | 369 | 444 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 6.3 | 7.1 | 6.7 | 6.9 | 5.6 | 5.3 | 5.7 | 4.7 | 4.4 | 4.4 | 4.9 | 6.9 | 7.0 | 7.2 | 8.4 | 8.8 | 8.4 | 6.5 | 6.7 | 7.0 |
Std Deviation (1e-4) | 1.3 | 0.9 | 1.1 | 1.2 | 1.7 | 1.1 | 0.9 | 1.3 | 1.2 | 0.8 | 0.9 | 1.1 | 1.0 | 1.2 | 1.5 | 1.3 | 1.4 | 1.4 | 1.1 | 1.6 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.03 | -0.34 | -0.41 | -0.31 | -0.58 | -0.68 | -0.7 | -0.54 | -0.04 | -0.01 | 0.03 | 0.25 | 0.32 | 0.24 | -0.12 | -0.09 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.3 | 1.5 | -1.4 | -1.6 | -3.6 | 0 | 0 | 0 | 0 | -1.0 | -1.6 | -3.2 | -1.4 | -0.4 | -0.6 |
Bud | 1.6 | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | -4.2 | -3.7 | -2.6 | -3.1 | -3.5 |
Bud Neck | -0.2 | -0.3 | 0 | 0.1 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 |
Bud Site | 2.5 | -1.7 | 0.3 | -0.7 | -3.5 | 0 | 0 | 0 | 0 | -0.7 | -4.5 | -5.8 | -2.3 | -2.6 | -2.9 |
Cell Periphery | -1.9 | -1.6 | -1.7 | -2.3 | -2.2 | 0 | 0 | 0 | 0 | 2.7 | 5.9 | 6.7 | 1.5 | 3.8 | 1.0 |
Cytoplasm | -4.1 | -0.9 | -0.8 | 0.4 | 1.8 | 2.1 | -0.1 | 1.5 | 1.0 | 2.7 | 6.2 | 7.0 | 0.9 | 1.9 | 2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 4.2 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.3 | 4.8 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.2 | 0.5 | 1.5 | 1.3 | -0.8 | -0.5 | 0.5 | -1.0 | 0.9 | 0 | 0 | 0 | 0.6 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 |
SpindlePole | 2.3 | 1.7 | -1.9 | -0.9 | -0.7 | 0 | 0 | 0 | 0 | -3.3 | -6.9 | -7.0 | -1.6 | -1.9 | -2.6 |
Vacuole | -0.1 | -0.2 | 0 | -2.7 | -0.6 | -3.5 | -2.0 | -2.1 | -3.7 | -3.2 | -4.8 | -4.0 | 0.9 | 1.9 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2196 | 4.3173 | 4.4824 | 4.1026 | 4.0265 | 4.8025 | 3.8284 | 3.1607 | 3.438 | 3.3591 | 3.1432 | 3.5376 | 3.071 | 2.569 | 2.8838 | 2.6911 | 2.6147 | 3.0755 |
Actin | 0.0557 | 0.02 | 0.0283 | 0.0388 | 0.0891 | 0.0232 | 0.0598 | 0.0359 | 0.0454 | 0.1314 | 0.2011 | 0.0454 | 0.0657 | 0.0165 | 0.0614 | 0.0566 | 0.0945 | 0.0439 |
Bud | 0.0527 | 0.0746 | 0.299 | 0.1051 | 0.0522 | 0.0178 | 0.0521 | 0.0559 | 0.3035 | 0.0965 | 0.0778 | 0.0139 | 0.059 | 0.0532 | 0.2666 | 0.1161 | 0.0542 | 0.0101 |
Bud Neck | 0.034 | 0.0086 | 0.0028 | 0.0358 | 0.0129 | 0.3249 | 0.0507 | 0.0054 | 0.0036 | 0.0047 | 0.0018 | 0.3977 | 0.0326 | 0.006 | 0.0067 | 0.0039 | 0.047 | 0.466 |
Bud Periphery | 0.1206 | 0.0802 | 0.4404 | 0.4248 | 0.3303 | 0.0609 | 0.1104 | 0.0624 | 0.4473 | 0.5039 | 0.3318 | 0.0515 | 0.094 | 0.0527 | 0.4173 | 0.4361 | 0.3713 | 0.0274 |
Bud Site | 0.2228 | 0.4319 | 0.0707 | 0.0191 | 0.0185 | 0.0262 | 0.2171 | 0.4622 | 0.0926 | 0.0499 | 0.0201 | 0.0142 | 0.205 | 0.3703 | 0.1042 | 0.0723 | 0.0156 | 0.0195 |
Cell Periphery | 0.029 | 0.0191 | 0.0077 | 0.0211 | 0.02 | 0.0087 | 0.0246 | 0.0065 | 0.0059 | 0.0107 | 0.0103 | 0.0107 | 0.0191 | 0.0075 | 0.0069 | 0.0141 | 0.015 | 0.0093 |
Cytoplasm | 0.2968 | 0.3053 | 0.0657 | 0.1322 | 0.2074 | 0.4015 | 0.2552 | 0.3052 | 0.0629 | 0.1439 | 0.2446 | 0.3533 | 0.2829 | 0.4016 | 0.0687 | 0.2827 | 0.2269 | 0.3449 |
Cytoplasmic Foci | 0.0185 | 0.005 | 0.0087 | 0.012 | 0.0314 | 0.0518 | 0.0586 | 0.0137 | 0.0034 | 0.002 | 0.0032 | 0.028 | 0.0631 | 0.0094 | 0.008 | 0.0012 | 0.033 | 0.0093 |
Eisosomes | 0.0009 | 0.0003 | 0.0004 | 0.003 | 0.0005 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0066 | 0.0019 | 0.0041 | 0.0103 | 0.0057 | 0.0015 | 0.0094 | 0.0015 | 0.0018 | 0.0061 | 0.0056 | 0.0065 | 0.0104 | 0.0026 | 0.0015 | 0.0016 | 0.0238 | 0.0068 |
Endosome | 0.0178 | 0.0025 | 0.0107 | 0.0312 | 0.0811 | 0.0186 | 0.0384 | 0.0025 | 0.0051 | 0.0089 | 0.0201 | 0.0207 | 0.0212 | 0.0015 | 0.0139 | 0.001 | 0.0177 | 0.0112 |
Golgi | 0.0062 | 0.0025 | 0.0052 | 0.018 | 0.0272 | 0.0046 | 0.0145 | 0.0016 | 0.0016 | 0.0056 | 0.005 | 0.0123 | 0.0056 | 0.0005 | 0.0079 | 0.0003 | 0.0061 | 0.0121 |
Lipid Particles | 0.0067 | 0.0003 | 0.0023 | 0.0073 | 0.0174 | 0.0042 | 0.0226 | 0.0007 | 0.0003 | 0.0021 | 0.0021 | 0.0049 | 0.0088 | 0.0003 | 0.0026 | 0.0002 | 0.0189 | 0.0047 |
Mitochondria | 0.0101 | 0.0081 | 0.0062 | 0.0284 | 0.0143 | 0.0023 | 0.0053 | 0.0021 | 0.002 | 0.0029 | 0.0081 | 0.0048 | 0.0078 | 0.0003 | 0.0054 | 0.0003 | 0.0135 | 0.0015 |
None | 0.08 | 0.0312 | 0.0328 | 0.0917 | 0.0262 | 0.037 | 0.0208 | 0.0305 | 0.0101 | 0.0029 | 0.0521 | 0.0154 | 0.0548 | 0.069 | 0.0133 | 0.0022 | 0.0051 | 0.0207 |
Nuclear Periphery | 0.0094 | 0.0004 | 0.0015 | 0.0014 | 0.0051 | 0.0004 | 0.0114 | 0.0004 | 0.0053 | 0.0092 | 0.0065 | 0.0023 | 0.0083 | 0.0004 | 0.0005 | 0.0005 | 0.0211 | 0.0042 |
Nucleolus | 0.0016 | 0.0003 | 0.0011 | 0.0006 | 0.0008 | 0.0004 | 0.003 | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0017 | 0.0003 | 0.0003 | 0.0002 | 0.0017 | 0.0003 |
Nucleus | 0.0034 | 0.0017 | 0.0013 | 0.0014 | 0.0017 | 0.0012 | 0.0162 | 0.0025 | 0.004 | 0.0016 | 0.002 | 0.003 | 0.0067 | 0.0015 | 0.0006 | 0.0011 | 0.0041 | 0.0009 |
Peroxisomes | 0.0078 | 0.0006 | 0.0026 | 0.0055 | 0.0411 | 0.0078 | 0.011 | 0.0038 | 0.0014 | 0.0063 | 0.0004 | 0.0051 | 0.019 | 0.0003 | 0.0079 | 0.0001 | 0.0065 | 0.0016 |
Punctate Nuclear | 0.0059 | 0.0005 | 0.0021 | 0.0005 | 0.003 | 0.0018 | 0.007 | 0.0009 | 0.0007 | 0.0005 | 0.0006 | 0.001 | 0.02 | 0.0007 | 0.0006 | 0.0001 | 0.006 | 0.0011 |
Vacuole | 0.0122 | 0.0046 | 0.0052 | 0.0104 | 0.0101 | 0.0038 | 0.0095 | 0.0048 | 0.0022 | 0.0069 | 0.0048 | 0.0077 | 0.0121 | 0.0047 | 0.004 | 0.0088 | 0.0147 | 0.0037 |
Vacuole Periphery | 0.0015 | 0.0002 | 0.001 | 0.0014 | 0.0039 | 0.0005 | 0.0017 | 0.0004 | 0.0005 | 0.0031 | 0.0014 | 0.001 | 0.0012 | 0.0002 | 0.0016 | 0.0004 | 0.003 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.1257 | 12.8709 | 18.8121 | 23.9694 | 12.7279 | 11.7736 | 17.9432 | 21.6923 | 19.6593 | 17.6057 |
Translational Efficiency | 0.7314 | 0.9132 | 0.8881 | 0.6846 | 0.6892 | 1.0805 | 0.7809 | 0.6645 | 0.9169 | 0.8185 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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