Standard name
Human Ortholog
Description Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.49 0.51 0.79 0.37 0.47 0.42 0.52 – 0.57 0.66 0.59 0.68 0.48 0.49 0.08 0.07 0.09 0.05 0.11 0.13 0.18 0.15 0.13
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome – 0 0 0 0.06 0 0 0 – 0 0 0 0 0.09 0.09 0 0 0 0 0.08 0.05 0 0 0.06
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0.1 0.05 0.13 0.12 0.13 0.1 – 0.08 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0.06 0.08 0.07 0 – 0 0 0 0 0 0 0.31 0.34 0.3 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.56 0.53 0.26 0.58 0.49 0.49 0.47 – 0.45 0.38 0.45 0.36 0.5 0.52 0.74 0.79 0.77 0.72 0.73 0.7 0.71 0.67 0.71
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 3 3
Bud – 0 0 2 0 0 3 5 – 1 8 1 0 0 0 0 0 0 0 8 1 3 8 10
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 2 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 1 – 3 1 1 1 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm – 174 68 143 80 112 138 173 – 226 219 243 167 221 245 36 24 31 1 41 19 48 58 54
Endoplasmic Reticulum – 4 1 1 0 4 3 2 – 4 2 1 8 4 4 19 18 13 0 1 0 1 5 1
Endosome – 15 5 5 13 1 0 1 – 2 0 1 5 39 46 8 1 6 1 30 8 7 17 23
Golgi – 0 2 0 0 0 0 0 – 0 0 1 0 0 1 0 0 2 0 4 2 0 4 1
Mitochondria – 9 14 10 29 29 42 32 – 31 36 40 0 1 1 11 3 3 0 5 4 5 18 11
Nucleus – 11 0 2 12 18 24 14 – 9 10 12 6 15 12 134 116 103 1 5 0 2 3 4
Nuclear Periphery – 2 0 0 3 2 12 4 – 6 7 4 0 0 1 7 2 1 0 2 1 0 1 1
Nucleolus – 0 0 0 1 0 2 0 – 1 0 0 0 2 0 1 0 1 0 0 0 0 2 1
Peroxisomes – 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 1 0 1 0 – 0 0 0 0 3 0 1 1 1 0 1 1 2 3 4
Vac/Vac Membrane – 198 71 48 124 116 161 158 – 179 127 183 89 228 264 321 272 259 23 280 103 194 270 300
Unique Cell Count 354 134 182 215 236 330 333 395 330 409 247 457 503 433 346 338 32 387 148 273 403 423
Labelled Cell Count 414 161 212 263 282 386 390 462 410 487 277 514 575 540 437 420 32 387 148 273 403 423


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.2 4.5 4.3 5.3 5.3 5.5 5.7 – 5.9 5.6 6.2 5.9 6.2 6.3 8.9 8.9 9.0 6.0 6.4 6.4
Std Deviation (1e-4) – 1.2 1.6 1.7 1.6 1.2 1.5 1.4 – 1.7 1.4 1.9 1.3 1.4 1.4 2.3 2.4 2.4 1.2 1.7 1.5
Intensity Change (Log2) – – – -0.05 0.26 0.26 0.31 0.35 – 0.41 0.34 0.47 0.41 0.46 0.51 0.99 0.99 1.01 0.43 0.51 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 5.2 -2.5 -0.6 -1.8 0.2 – 1.3 3.1 1.8 3.2 -0.5 -0.4 -11.1 -10.9 -10.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 2.2 0
Endosome 0 1.0 0 0 0 – 0 0 0 0 1.9 2.1 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.8 0.5 0.7 -0.3 – -0.9 0.1 -0.2 0 0 0 -3.9 0 0
Nucleus 0 0 3.3 3.2 0 – 0 0 0 0 0 0 7.4 7.7 7.2
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -4.8 0.9 -0.7 -0.8 -1.1 – -1.5 -2.9 -1.7 -3.2 -0.6 -0.1 4.6 5.6 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1524 0.4369 0.149 -0.1243 0.5206 -0.0279 2.8494 2.5868 2.8919 2.1804 2.9689 3.0103 -0.2002 0.9351 0.3989 0.2308 -0.5287 0.2887
Actin 0.0261 0.0002 0.0033 0.0051 0.0311 0.0055 0.0226 0.0008 0.004 0 0.0233 0.0031 0.0011 0.0001 0.0002 0 0.0001 0.0001
Bud 0.0014 0.0028 0.0063 0.0058 0.0071 0.0007 0.0017 0.0058 0.0054 0.0012 0.0039 0.0017 0.0007 0.0062 0.0007 0.0002 0.0004 0.0005
Bud Neck 0.0018 0.0011 0.002 0.0007 0.0027 0.0018 0.0043 0.0014 0.0026 0.0007 0.0019 0.0024 0.001 0.0009 0.0008 0.0004 0.0016 0.0016
Bud Periphery 0.0013 0.0027 0.0056 0.0115 0.0278 0.0011 0.0024 0.0102 0.0127 0.0023 0.0155 0.0032 0.0015 0.0027 0.001 0.0003 0.0007 0.0012
Bud Site 0.0028 0.0111 0.0152 0.0023 0.0055 0.0006 0.0057 0.0138 0.0079 0.0007 0.0024 0.001 0.0019 0.0089 0.0019 0.0004 0.0011 0.0015
Cell Periphery 0.0007 0.0011 0.0008 0.0003 0.0044 0.0004 0.0013 0.0011 0.0014 0.0004 0.0007 0.0005 0.001 0.0011 0.0007 0.0003 0.0006 0.0005
Cytoplasm 0.0774 0.1293 0.104 0.0778 0.0839 0.1422 0.0899 0.0788 0.0824 0.0591 0.0918 0.0897 0.0656 0.0958 0.0652 0.0911 0.1346 0.0586
Cytoplasmic Foci 0.084 0.0462 0.0479 0.0801 0.0603 0.0679 0.0524 0.0532 0.0499 0.0381 0.0615 0.0615 0.05 0.0398 0.0459 0.0224 0.0242 0.0407
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0005 0 0.0002 0.0001 0.0004 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0057 0.0044 0.0034 0.0022 0.0023 0.0024 0.0074 0.0031 0.0056 0.0025 0.0045 0.003 0.0059 0.0048 0.0043 0.0017 0.0061 0.0029
Endosome 0.199 0.127 0.1936 0.2788 0.1567 0.1903 0.2147 0.2048 0.2199 0.2104 0.2532 0.2537 0.2619 0.1944 0.2651 0.2248 0.3379 0.2886
Golgi 0.0159 0.0019 0.0045 0.0161 0.0329 0.0039 0.0093 0.0055 0.0049 0.0017 0.0167 0.0047 0.0052 0.0023 0.0046 0.0031 0.0066 0.0038
Lipid Particles 0.0396 0.0105 0.0101 0.0088 0.0524 0.0104 0.0169 0.0028 0.007 0.0018 0.0154 0.0068 0.0162 0.0095 0.0104 0.0019 0.0088 0.0082
Mitochondria 0.0191 0.005 0.0234 0.0307 0.0503 0.0058 0.0164 0.0062 0.0146 0.0061 0.0213 0.0079 0.0065 0.0021 0.0047 0.0008 0.0039 0.0072
None 0.2742 0.3666 0.2913 0.2297 0.1913 0.212 0.2068 0.1536 0.1789 0.2857 0.1363 0.1548 0.1302 0.1261 0.1704 0.1137 0.0083 0.0835
Nuclear Periphery 0.0135 0.0082 0.0045 0.0032 0.0021 0.0035 0.0097 0.0034 0.0086 0.0046 0.0054 0.0051 0.0086 0.0107 0.0046 0.0029 0.0055 0.0066
Nucleolus 0.0038 0.0042 0.0075 0.0018 0.0031 0.0038 0.0045 0.0044 0.007 0.0016 0.0046 0.0022 0.0054 0.0041 0.003 0.0009 0.002 0.0025
Nucleus 0.0212 0.0126 0.0176 0.007 0.0044 0.0086 0.0087 0.0089 0.0111 0.0054 0.0073 0.0079 0.0181 0.0122 0.0069 0.0031 0.005 0.0084
Peroxisomes 0.0175 0.0012 0.0031 0.0111 0.0185 0.0099 0.004 0.0016 0.0022 0.0008 0.0068 0.0042 0.0071 0.0022 0.0021 0.0002 0.0006 0.0017
Punctate Nuclear 0.0042 0.0021 0.0043 0.0011 0.002 0.0061 0.0039 0.0023 0.0029 0.0009 0.0167 0.002 0.0031 0.0015 0.0014 0.0005 0.0005 0.0028
Vacuole 0.1732 0.2313 0.2189 0.189 0.2274 0.2841 0.2826 0.3878 0.3204 0.3344 0.2652 0.3284 0.3518 0.4151 0.3453 0.4541 0.3912 0.4022
Vacuole Periphery 0.0171 0.0304 0.0326 0.0368 0.0334 0.0388 0.0343 0.0505 0.0506 0.0417 0.0451 0.0563 0.0569 0.0594 0.0606 0.077 0.0604 0.0769

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.3018 20.9633 25.3803 27.1114 22.4033 38.24 29.2838 26.3528 38.3202 30.5124
Translational Efficiency 0.9404 1.1319 0.9586 1.3824 1.471 1.0875 1.2117 1.9206 1.0685 1.1653

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
Localization
Cell Percentages vacuole (36%), cytoplasm (9%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bxi1

Bxi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bxi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available