Standard name | |
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Human Ortholog | |
Description | Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 3 | 4 |
Bud | 1 | 0 | 2 | 1 | 1 | 2 | 1 | 2 | 8 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 8 | 4 | 2 | 5 | 5 |
Bud Neck | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 1 | 1 |
Bud Site | 0 | 2 | 1 | 1 | 0 | 4 | 0 | 6 | 24 | 10 | 8 | 22 | 2 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 5 | 7 | 8 | 10 | 15 | 14 | 4 | 6 | 4 | 6 | 6 | 8 | 7 | 12 | 20 | 20 | 20 | 0 | 3 | 2 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 4 | 5 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 2 | 6 | 24 | 5 | 2 | 19 | 27 | 23 | 6 | 26 | 13 | 4 | 16 | 25 | 31 | 15 | 3 | 6 | 9 | 17 | 43 | 15 | 32 | 47 |
Golgi | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 3 | 2 | 3 | 2 | 11 | 8 | 16 | 17 |
Mitochondria | 16 | 15 | 10 | 3 | 11 | 24 | 21 | 29 | 67 | 32 | 39 | 33 | 5 | 8 | 12 | 14 | 9 | 6 | 2 | 12 | 20 | 11 | 25 | 20 |
Nucleus | 45 | 186 | 138 | 103 | 108 | 128 | 104 | 80 | 79 | 110 | 83 | 118 | 106 | 110 | 81 | 196 | 170 | 175 | 46 | 181 | 121 | 55 | 69 | 70 |
Nuclear Periphery | 0 | 10 | 6 | 3 | 6 | 2 | 8 | 5 | 3 | 6 | 5 | 4 | 4 | 0 | 0 | 3 | 4 | 0 | 2 | 8 | 8 | 0 | 2 | 3 |
Nucleolus | 40 | 66 | 57 | 64 | 39 | 99 | 90 | 135 | 123 | 131 | 110 | 132 | 33 | 35 | 44 | 50 | 43 | 29 | 13 | 41 | 34 | 4 | 12 | 14 |
Peroxisomes | 0 | 7 | 24 | 5 | 5 | 20 | 27 | 17 | 41 | 28 | 18 | 24 | 14 | 23 | 45 | 10 | 3 | 2 | 1 | 2 | 19 | 9 | 40 | 42 |
SpindlePole | 4 | 10 | 49 | 11 | 12 | 29 | 30 | 48 | 72 | 65 | 41 | 44 | 25 | 28 | 40 | 10 | 6 | 6 | 15 | 44 | 42 | 14 | 33 | 47 |
Vac/Vac Membrane | 3 | 14 | 13 | 8 | 3 | 9 | 7 | 12 | 7 | 9 | 4 | 4 | 10 | 7 | 7 | 16 | 27 | 28 | 9 | 34 | 28 | 24 | 40 | 38 |
Unique Cell Count | 96 | 292 | 274 | 186 | 177 | 295 | 267 | 299 | 317 | 352 | 260 | 310 | 184 | 200 | 199 | 297 | 267 | 257 | 113 | 369 | 352 | 151 | 294 | 319 |
Labelled Cell Count | 115 | 325 | 333 | 212 | 197 | 352 | 330 | 364 | 439 | 428 | 333 | 392 | 228 | 244 | 272 | 339 | 292 | 280 | 113 | 369 | 352 | 151 | 294 | 319 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.6 | 11.6 | 11.9 | 11.6 | 10.6 | 13.7 | 13.5 | 13.2 | 11.4 | 13.4 | 11.8 | 12.8 | 16.0 | 13.9 | 14.6 | 14.9 | 18.6 | 17.5 | 11.4 | 11.3 | 11.2 |
Std Deviation (1e-4) | 8.3 | 5.0 | 4.1 | 3.8 | 3.3 | 4.1 | 4.4 | 5.0 | 4.3 | 6.1 | 4.6 | 5.3 | 8.5 | 6.0 | 7.2 | 6.4 | 9.8 | 8.4 | 3.3 | 4.7 | 4.5 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.18 | 0.2 | 0.17 | 0.15 | -0.07 | 0.16 | -0.02 | 0.1 | 0.42 | 0.22 | 0.29 | 0.32 | 0.64 | 0.55 | -0.06 | -0.09 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 1.7 | 1.6 | 1.6 | -1.1 | -0.5 | 0 | -0.2 | -0.5 | 1.1 | 0.6 | 1.9 | 2.4 | 2.6 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.6 | -3.4 | -1.0 | 0.5 | -0.5 | -3.8 | -0.6 | -1.7 | -4.2 | 0 | 1.3 | 2.3 | -1.8 | -4.1 | -3.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.3 | 1.3 | 2.3 | 2.1 | 2.9 | 6.3 | 2.7 | 4.5 | 3.2 | -0.5 | 0.2 | 1.2 | 0.6 | -0.2 | -0.9 |
Nucleus | 1.1 | 2.2 | -1.7 | -2.7 | -5.8 | -6.4 | -4.9 | -4.3 | -3.0 | 1.5 | 1.0 | -2.1 | 3.8 | 3.1 | 4.2 |
Nuclear Periphery | 0 | 0 | 0 | 0.6 | -0.5 | 0 | -0.4 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.3 | 0.3 | 3.4 | 3.4 | 6.2 | 4.7 | 4.4 | 5.4 | 5.6 | -0.8 | -0.9 | 0.3 | -1.2 | -1.4 | -3.0 |
Peroxisomes | -2.6 | -2.5 | -0.9 | 0.5 | -1.4 | 1.6 | -0.4 | -0.8 | -0.4 | -0.4 | 1.0 | 4.2 | -2.7 | -4.1 | -4.3 |
SpindlePole | -3.7 | -3.4 | -2.8 | -2.2 | -0.6 | 1.5 | 0.2 | -0.7 | -1.2 | -1.2 | -1.1 | 0.6 | -5.7 | -6.0 | -5.9 |
Vacuole | -0.2 | -1.7 | -1.0 | -1.3 | -0.4 | -1.7 | -1.5 | -2.1 | -2.5 | 0.3 | -0.7 | -0.7 | 0.3 | 2.4 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.1237 | 12.1404 | 12.3346 | 10.9268 | 11.1925 | 11.2092 | 11.7552 | 13.3108 | 14.2879 | 13.8522 | 12.8186 | 12.4495 | 8.1266 | 13.2745 | 9.6761 | 9.2147 | 14.8609 | 9.7702 |
Actin | 0.0259 | 0.001 | 0.0039 | 0.0101 | 0.0156 | 0.0096 | 0.0026 | 0.0025 | 0.0067 | 0.0005 | 0.0263 | 0.0067 | 0.0758 | 0.0213 | 0.0098 | 0.0581 | 0.0081 | 0.0023 |
Bud | 0.0006 | 0.0001 | 0.0002 | 0.0007 | 0.0009 | 0.0013 | 0 | 0.0009 | 0.0007 | 0.0001 | 0.0006 | 0.002 | 0.0007 | 0.0002 | 0.0002 | 0.0018 | 0.0005 | 0.0005 |
Bud Neck | 0.0042 | 0.0003 | 0.0002 | 0.0007 | 0.0027 | 0.0075 | 0.0006 | 0.0014 | 0.001 | 0.0001 | 0.0005 | 0.0099 | 0.0017 | 0.0006 | 0.0002 | 0.0005 | 0.0009 | 0.0029 |
Bud Periphery | 0.0008 | 0.0005 | 0.0006 | 0.0012 | 0.0024 | 0.0058 | 0 | 0.0014 | 0.0022 | 0.0004 | 0.0011 | 0.0028 | 0.0011 | 0.0005 | 0.0004 | 0.0027 | 0.0008 | 0.0015 |
Bud Site | 0.0112 | 0.0003 | 0.0003 | 0.0028 | 0.0023 | 0.0012 | 0.0002 | 0.0009 | 0.0017 | 0.0005 | 0.0005 | 0.0029 | 0.0015 | 0.001 | 0.0006 | 0.0023 | 0.0007 | 0.001 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0012 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 |
Cytoplasm | 0.0065 | 0.0002 | 0.0001 | 0.0033 | 0.0179 | 0.0004 | 0.0132 | 0.0025 | 0.001 | 0.0001 | 0.0015 | 0.0073 | 0.0043 | 0.0022 | 0.0004 | 0.0017 | 0.0017 | 0.0002 |
Cytoplasmic Foci | 0.0116 | 0.0021 | 0.0046 | 0.0237 | 0.1456 | 0.0042 | 0.0031 | 0.0091 | 0.0055 | 0.1514 | 0.0014 | 0.0087 | 0.0195 | 0.022 | 0.0259 | 0.0429 | 0.2121 | 0.0017 |
Eisosomes | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0047 | 0.0001 | 0.0001 | 0.0032 | 0.0099 | 0.0006 | 0.0006 | 0.0008 | 0.0005 | 0.0001 | 0.0021 | 0.0012 | 0.0049 | 0.0006 | 0.0003 | 0.0003 | 0.0008 | 0.0001 |
Endosome | 0.0246 | 0.0027 | 0.0003 | 0.0108 | 0.0144 | 0.004 | 0.0008 | 0.0229 | 0.0022 | 0.0016 | 0.0046 | 0.0042 | 0.0516 | 0.0029 | 0.0044 | 0.0057 | 0.0048 | 0.0008 |
Golgi | 0.0124 | 0.0128 | 0.0005 | 0.003 | 0.0051 | 0.0034 | 0.0003 | 0.0152 | 0.0018 | 0.0022 | 0.0016 | 0.0025 | 0.0433 | 0.016 | 0.0068 | 0.0123 | 0.0031 | 0.001 |
Lipid Particles | 0.0185 | 0.0032 | 0.0088 | 0.0215 | 0.0398 | 0.0227 | 0.0006 | 0.0034 | 0.013 | 0.0588 | 0.0053 | 0.0154 | 0.0124 | 0.0295 | 0.0651 | 0.0254 | 0.136 | 0.01 |
Mitochondria | 0.0131 | 0.0016 | 0.0098 | 0.01 | 0.0063 | 0.0378 | 0.0004 | 0.0491 | 0.0099 | 0.0112 | 0.0534 | 0.008 | 0.0113 | 0.0041 | 0.0188 | 0.0278 | 0.0059 | 0.0062 |
None | 0.012 | 0.0001 | 0.0002 | 0.0018 | 0.0034 | 0.0005 | 0.2148 | 0.0003 | 0.001 | 0.0003 | 0.0012 | 0.0115 | 0.0023 | 0.0104 | 0.0008 | 0.0021 | 0.0013 | 0.0002 |
Nuclear Periphery | 0.0296 | 0.0633 | 0.0995 | 0.0698 | 0.0837 | 0.0199 | 0.0014 | 0.02 | 0.0191 | 0.006 | 0.1553 | 0.0134 | 0.0236 | 0.0256 | 0.0122 | 0.0245 | 0.0315 | 0.0222 |
Nucleolus | 0.0815 | 0.1311 | 0.1434 | 0.1172 | 0.0869 | 0.1567 | 0.0401 | 0.1227 | 0.1402 | 0.0339 | 0.0097 | 0.201 | 0.1343 | 0.2078 | 0.1715 | 0.1701 | 0.0843 | 0.2735 |
Nucleus | 0.637 | 0.6331 | 0.5579 | 0.5566 | 0.3326 | 0.6536 | 0.7105 | 0.6432 | 0.7146 | 0.5504 | 0.5709 | 0.6499 | 0.4593 | 0.4948 | 0.5518 | 0.412 | 0.4081 | 0.5264 |
Peroxisomes | 0.0124 | 0.0002 | 0.0004 | 0.0076 | 0.0115 | 0.0071 | 0.0004 | 0.0056 | 0.0017 | 0.0083 | 0.0018 | 0.0015 | 0.0678 | 0.0074 | 0.0071 | 0.0377 | 0.0044 | 0.0023 |
Punctate Nuclear | 0.0881 | 0.1435 | 0.1656 | 0.1519 | 0.2078 | 0.0493 | 0.0092 | 0.0927 | 0.0724 | 0.1724 | 0.1545 | 0.0477 | 0.0785 | 0.1496 | 0.1185 | 0.1673 | 0.0928 | 0.1439 |
Vacuole | 0.0022 | 0.0008 | 0.0004 | 0.0015 | 0.0071 | 0.0055 | 0.0001 | 0.0022 | 0.0017 | 0.0005 | 0.0013 | 0.0016 | 0.0028 | 0.0007 | 0.0014 | 0.0012 | 0.0007 | 0.0011 |
Vacuole Periphery | 0.0021 | 0.0028 | 0.0032 | 0.0021 | 0.0034 | 0.0073 | 0.0001 | 0.0025 | 0.0024 | 0.001 | 0.0062 | 0.0012 | 0.0028 | 0.0024 | 0.0035 | 0.0032 | 0.0013 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 140.9458 | 55.1987 | 24.932 | 45.7668 | 79.1884 | 110.011 | 38.8253 | 24.1572 | 40.3943 | 87.6369 |
Translational Efficiency | 1.8397 | 1.6098 | 1.84 | 1.9614 | 2.1782 | 2.5044 | 1.862 | 2.1328 | 1.9934 | 1.9478 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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