Standard name
Human Ortholog
Description Prephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.98 0.95 0.88 0.78 0.85 0.71 0.76 0.66 0.7 0.98 1.0 1.0 – – – 0.92 0.94 0.9 0.94 0.94 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.27 0.19 0.4 0.3 0.49 0.29 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.08 0 0.06 0.05 0 0.1 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – 1 1 0 0 0 0
Bud 0 0 0 0 0 0 5 2 5 6 8 10 0 0 0 – – – 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 0 1 0 0 3 4 2 2 4 4 3 1 0 0 – – – 0 0 0 0 0 0
Cytoplasm 261 136 169 198 341 239 282 296 272 276 180 212 186 144 210 – – – 251 150 161 146 187 222
Endoplasmic Reticulum 3 0 0 0 0 1 0 0 2 0 1 2 3 0 1 – – – 1 0 1 0 0 0
Endosome 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 – – – 7 1 1 0 0 0
Golgi 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 – – – 0 1 2 0 0 0
Mitochondria 6 2 0 4 6 34 99 65 154 109 133 89 0 0 0 – – – 4 1 2 2 0 1
Nucleus 0 0 0 0 3 6 3 5 9 14 5 6 1 0 2 – – – 0 2 1 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 1 0 0 0
Vac/Vac Membrane 0 0 2 6 23 11 28 15 22 17 12 29 1 0 1 – – – 0 2 2 3 2 1
Unique Cell Count 268 138 172 203 358 271 363 347 382 365 273 305 189 144 211 274 161 179 155 199 231
Labelled Cell Count 272 138 174 209 374 294 421 387 468 427 343 352 192 144 214 274 161 179 155 199 231


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 7.2 6.7 6.4 6.4 5.3 4.8 5.4 4.3 4.6 4.3 4.8 7.1 7.1 7.4 – – – 6.4 6.9 7.3
Std Deviation (1e-4) 0.5 0.9 1.2 1.5 1.6 1.7 1.5 1.6 0.9 0.9 0.9 0.7 0.9 1.2 1.4 – – – 1.0 1.5 1.3
Intensity Change (Log2) – – – -0.06 -0.05 -0.32 -0.47 -0.31 -0.64 -0.52 -0.63 -0.47 0.09 0.08 0.15 – – – -0.05 0.04 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm -0.5 -1.7 -3.8 -6.1 -4.5 -7.3 -6.5 -8.1 -7.5 0.1 1.6 1.2 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 4.8 0 6.1 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 2.7 1.8 3.1 1.9 2.5 2.0 1.9 3.6 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6885 3.3722 2.9453 2.612 2.3245 2.9542 1.1302 2.0612 1.7073 1.263 1.304 1.7596 0.8908 1.703 1.3876 1.4216 1.0871 1.4427
Actin 0.0096 0.0004 0.0004 0.0119 0.0033 0.0036 0.0131 0.0011 0.0044 0.0047 0.0008 0.0134 0.0268 0.0033 0.0237 0.0009 0.0002 0.0071
Bud 0.0007 0.0003 0.0003 0.001 0.0009 0.0009 0.0002 0.0012 0.0059 0.0006 0.0004 0.0003 0.0005 0.0001 0.0009 0.0012 0 0.0003
Bud Neck 0.0026 0.0003 0.0003 0.0012 0.0014 0.0017 0.0009 0.0003 0.0065 0.001 0.0008 0.0062 0.0066 0.0008 0.0009 0.0005 0.0003 0.0009
Bud Periphery 0.001 0.0001 0.0001 0.0011 0.0009 0.0007 0.0002 0.0002 0.0022 0.0012 0.0001 0.0003 0.0004 0.0001 0.0006 0.0003 0 0.0002
Bud Site 0.0048 0.0026 0.0011 0.0011 0.0029 0.0015 0.0011 0.0057 0.0049 0.0024 0.0004 0.0003 0.0073 0.0016 0.0189 0.0008 0 0.0003
Cell Periphery 0.0002 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.6838 0.7976 0.7676 0.6923 0.7058 0.7731 0.783 0.8229 0.7702 0.8186 0.6627 0.7752 0.6969 0.9018 0.8218 0.7083 0.9144 0.9016
Cytoplasmic Foci 0.0177 0.01 0.008 0.0292 0.0321 0.0181 0.0191 0.009 0.0164 0.0115 0.0642 0.0259 0.0512 0.01 0.016 0.0383 0.0064 0.0125
Eisosomes 0.0006 0 0 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0032 0.0021 0.0016 0.003 0.0016 0.0015 0.0035 0.0031 0.0027 0.0042 0.0045 0.0023 0.0031 0.0018 0.0025 0.0047 0.0032 0.0033
Endosome 0.0093 0.005 0.0029 0.0165 0.0133 0.0057 0.0279 0.0045 0.0112 0.0088 0.0104 0.017 0.045 0.0052 0.014 0.0754 0.0012 0.0101
Golgi 0.0019 0.0003 0.0002 0.0056 0.0044 0.0003 0.004 0.0005 0.0017 0.004 0.0004 0.0043 0.0159 0.0038 0.0072 0.014 0.0001 0.0014
Lipid Particles 0.0041 0.0001 0.0001 0.0077 0.0048 0.0001 0.0065 0.0021 0.0017 0.0105 0.0045 0.0041 0.0117 0.0007 0.0013 0.0069 0.0001 0.0009
Mitochondria 0.0017 0.0003 0.0004 0.0055 0.0045 0.0005 0.0017 0.0006 0.0009 0.0217 0.0031 0.0011 0.0092 0.0016 0.0041 0.0112 0.0001 0.0008
None 0.2204 0.1675 0.1893 0.1759 0.1775 0.1758 0.0987 0.1233 0.138 0.0778 0.1301 0.1115 0.071 0.0609 0.0717 0.0909 0.0671 0.0349
Nuclear Periphery 0.0022 0.0014 0.0014 0.0063 0.0021 0.0011 0.0058 0.0016 0.0027 0.0027 0.0119 0.0048 0.0039 0.0008 0.0014 0.0021 0.0011 0.0118
Nucleolus 0.0007 0.0001 0.0001 0.0005 0.0011 0.0001 0.0009 0.0004 0.0005 0.0002 0.0043 0.0002 0.0006 0 0.0001 0.001 0 0.0001
Nucleus 0.0172 0.0077 0.023 0.0171 0.0125 0.0099 0.0175 0.0069 0.0159 0.007 0.0411 0.0162 0.0152 0.0038 0.0036 0.0067 0.005 0.006
Peroxisomes 0.0105 0.0003 0.0003 0.0134 0.012 0.0012 0.0029 0.0085 0.0025 0.0173 0.0014 0.0031 0.0202 0.0023 0.0081 0.0098 0 0.0038
Punctate Nuclear 0.0056 0.0027 0.0019 0.0085 0.0174 0.0037 0.0095 0.0067 0.0095 0.0025 0.0564 0.0123 0.0108 0.0006 0.0024 0.0173 0.0004 0.0019
Vacuole 0.0016 0.0011 0.0008 0.0015 0.0011 0.0006 0.0028 0.001 0.0016 0.0023 0.0017 0.001 0.0026 0.0005 0.0006 0.0078 0.0003 0.0009
Vacuole Periphery 0.0003 0.0002 0.0001 0.0005 0.0003 0.0001 0.0008 0.0002 0.0003 0.0006 0.0005 0.0004 0.0007 0.0001 0.0002 0.0017 0.0001 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.175 11.7776 9.4678 10.9981 11.388 4.3892 11.9083 15.07 10.8382 12.0827
Translational Efficiency 1.2156 1.1102 1.1346 1.1097 0.9816 3.2747 1.3202 0.8503 1.2395 1.3185

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Prephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pha2

Pha2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pha2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available