Standard name
Human Ortholog
Description Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.25 0.32 0.27 0.14 0.12 0.1 0.1 0.09 0.16 0.18 0.18 0.18 0.15 0.11 0.45 0.25 0.25 0 0.09 0.09 0.08 0.07 0.05
Bud 0.11 0 0.08 0.1 0 0 0 0 0.09 0.06 0.13 0.07 0 0.05 0 0.18 0.05 0.13 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – – – –
Cell Periphery 0.06 0 0.11 0.08 0.06 0.06 0.05 0.05 0.07 0.08 0.11 0.14 0.17 0.09 0.16 0.24 0.25 0.36 0 0 0 0 0 0
Cytoplasm 0.17 0.38 0.08 0.2 0.17 0.22 0.2 0.21 0.35 0.31 0.37 0.3 0.17 0.2 0.13 0.21 0.41 0.39 0 0.09 0 0 0 0
Endoplasmic Reticulum 0.67 0.5 0.67 0.51 0.72 0.6 0.61 0.49 0.3 0.39 0.22 0.35 0.64 0.69 0.75 0 0 0.07 0.79 0.64 0.74 0.72 0.8 0.77
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.12 0 0.09 0 0.13 0.11 0.14 0.21 0.19 0.28 0.22 0 0 0 0.18 0.1 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.08 0.06 0.09 0.15 0.11 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 140 109 63 66 71 62 35 79 69 114 64 78 48 17 50 19 2 7 49 33 52 43
Bud 2 1 34 40 11 22 18 25 35 28 50 44 15 25 14 7 11 10 0 2 8 12 12 21
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 2 4 17 19 18 16
Bud Site 0 0 1 2 1 2 1 4 3 6 5 6 0 1 0 2 5 0 – – – – – –
Cell Periphery 1 1 49 34 27 32 36 31 27 39 42 88 58 48 74 9 50 27 1 0 21 9 33 31
Cytoplasm 3 9 34 79 76 123 139 134 131 150 139 186 59 102 59 8 82 30 1 7 3 8 14 19
Endoplasmic Reticulum 12 12 296 206 329 337 415 316 110 188 81 217 220 354 341 1 5 5 50 50 389 289 628 626
Endosome 0 0 2 8 6 17 8 4 7 3 4 9 0 0 0 0 5 0 0 0 2 0 1 2
Golgi 0 0 1 6 2 1 1 0 0 1 0 1 0 1 0 0 3 1 0 0 1 2 0 3
Mitochondria 1 3 19 35 12 71 74 90 79 94 106 135 0 0 1 7 20 4 1 0 15 11 10 11
Nucleus 0 0 0 1 0 2 2 7 0 0 1 0 0 2 4 1 3 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 4 3 10 14 6 7 2 6 1 1 0 0 0 0 0 1 1 0 0 2
Nucleolus 0 0 0 0 0 0 3 9 4 1 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 3 3 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 2 0 0 0
SpindlePole 0 0 0 3 1 1 0 0 0 0 0 2 1 0 3 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 18 26 37 33 61 93 41 25 20 26 2 16 14 1 9 4 0 2 7 10 15 25
Unique Cell Count 18 24 443 405 457 564 682 641 370 484 374 627 346 515 455 38 202 76 64 78 523 400 790 811
Labelled Cell Count 24 32 597 553 569 710 839 790 478 621 523 836 421 629 558 54 245 102 64 78 523 400 790 811


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 10.2 9.9 10.0 10.0 8.9 8.7 8.9 8.5 8.2 8.0 8.3 11.3 11.5 12.6 8.2 9.7 12.2 12.6 12.9 12.2
Std Deviation (1e-4) 2.0 2.3 2.0 1.9 1.9 1.7 1.7 1.7 1.7 1.6 1.6 1.6 1.8 2.0 2.4 1.8 2.3 6.1 2.2 2.3 2.2
Intensity Change (Log2) – – – 0.01 0.01 -0.15 -0.19 -0.16 -0.22 -0.28 -0.3 -0.26 0.18 0.22 0.35 -0.27 -0.03 0.3 0.34 0.38 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -6.4 -7.8 -8.9 -9.1 -7.6 -5.5 -4.3 -5.1 -4.2 -6.1 -7.8 1.7 -1.8 -1.2
Bud 1.1 -3.6 -2.6 -3.9 -2.7 0.9 -1.2 2.7 -0.4 -1.9 -1.8 -3.1 0 -1.0 1.6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -2.8 -3.1 -3.6 -3.9 -1.8 -1.6 0.1 1.4 2.3 -0.9 2.3 0 4.5 5.6
Cytoplasm 5.1 4.1 6.1 5.8 5.9 9.8 8.9 10.3 8.8 4.1 5.4 2.6 0 10.1 7.8
Endoplasmic Reticulum -4.7 1.7 -2.3 -2.0 -5.7 -10.5 -8.5 -12.9 -10.4 -0.9 0.6 2.7 -7.8 -15.2 -9.8
Endosome 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 -1.4 4.6 3.9 5.2 7.4 7.0 9.5 7.9 -3.9 -4.7 -4.1 0 2.8 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.5 2.5 1.3 3.1 5.6 3.8 0.8 0.9 0.1 -3.1 -0.8 -0.8 0 0.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2955 5.1644 6.0386 5.4542 5.1218 5.5069 4.9254 4.2826 5.2766 4.2914 4.8962 5.2499 3.8267 4.8739 4.6715 4.4067 3.955 5.5715
Actin 0.0734 0.0354 0.0597 0.0485 0.0172 0.0429 0.0317 0.0818 0.1042 0.1341 0.0698 0.0703 0.063 0.0295 0.057 0.1156 0.1274 0.0305
Bud 0.0098 0.0246 0.0871 0.0448 0.0167 0.009 0.0114 0.0022 0.0503 0.0108 0.0131 0.0023 0.003 0.0018 0.0298 0.0144 0.0045 0.0013
Bud Neck 0.0343 0.0113 0.0138 0.0068 0.077 0.2911 0.0184 0.0071 0.0116 0.0307 0.0037 0.283 0.019 0.0114 0.0219 0.0049 0.0043 0.4779
Bud Periphery 0.0455 0.0559 0.2865 0.2636 0.1238 0.0427 0.0363 0.0042 0.1864 0.1216 0.1449 0.0094 0.0111 0.0035 0.1329 0.1018 0.0408 0.0119
Bud Site 0.074 0.1471 0.0799 0.0296 0.0068 0.0047 0.0322 0.1219 0.046 0.0053 0.002 0.0062 0.0797 0.2011 0.0321 0.02 0.0032 0.0013
Cell Periphery 0.0723 0.033 0.0286 0.0373 0.0252 0.0266 0.0461 0.018 0.0258 0.0535 0.0396 0.0249 0.0518 0.059 0.0401 0.0536 0.036 0.028
Cytoplasm 0.2255 0.3308 0.1443 0.1889 0.1551 0.2172 0.1991 0.2876 0.1404 0.281 0.2747 0.1619 0.2603 0.2782 0.2026 0.2021 0.4024 0.1669
Cytoplasmic Foci 0.0315 0.0106 0.0075 0.0297 0.0095 0.0081 0.0423 0.0123 0.0167 0.0156 0.0128 0.0121 0.0117 0.0076 0.013 0.0042 0.0427 0.0035
Eisosomes 0.0014 0.0009 0.0008 0.001 0.0013 0.0006 0.0012 0.0011 0.0008 0.0015 0.0011 0.0005 0.0025 0.0004 0.0007 0.0009 0.0014 0.0005
Endoplasmic Reticulum 0.2708 0.2017 0.1675 0.1781 0.3792 0.262 0.4192 0.3245 0.3002 0.2209 0.304 0.3622 0.297 0.2502 0.3081 0.3359 0.1531 0.2421
Endosome 0.0383 0.0259 0.0137 0.0132 0.0419 0.015 0.0184 0.0139 0.0227 0.0154 0.0217 0.0126 0.0164 0.0121 0.0181 0.0092 0.0777 0.0015
Golgi 0.0161 0.0074 0.0064 0.0076 0.0161 0.0067 0.0152 0.0086 0.0215 0.0208 0.0081 0.013 0.0191 0.0053 0.0192 0.0116 0.0265 0.0019
Lipid Particles 0.0244 0.0096 0.0042 0.013 0.0092 0.0053 0.034 0.0211 0.0233 0.0075 0.0117 0.0153 0.0204 0.0323 0.0167 0.0058 0.0315 0.0019
Mitochondria 0.0069 0.002 0.0078 0.0034 0.0017 0.002 0.0042 0.0006 0.0054 0.0042 0.0035 0.0058 0.0069 0.0007 0.0088 0.0031 0.0144 0.0002
None 0.0092 0.0681 0.054 0.0651 0.084 0.0407 0.0573 0.0682 0.018 0.0559 0.071 0.0069 0.1011 0.0731 0.0581 0.0918 0.0033 0.02
Nuclear Periphery 0.0232 0.0106 0.0111 0.0069 0.0068 0.0088 0.0078 0.0105 0.0098 0.0075 0.0075 0.0037 0.0147 0.0129 0.0121 0.0133 0.0081 0.0035
Nucleolus 0.003 0.0008 0.0009 0.0005 0.0003 0.0003 0.0009 0.0003 0.0002 0.0002 0.0001 0.0002 0.0005 0.0003 0.0004 0.0002 0.0017 0.0001
Nucleus 0.0054 0.0061 0.0044 0.0021 0.0019 0.0044 0.0018 0.0022 0.0019 0.0015 0.0016 0.0015 0.0022 0.0029 0.0038 0.0023 0.0038 0.0013
Peroxisomes 0.0034 0.0004 0.0062 0.0396 0.0002 0.0005 0.0077 0.0003 0.0008 0.0007 0.0003 0.0017 0.0004 0.0002 0.0033 0.0002 0.0021 0.0001
Punctate Nuclear 0.009 0.0013 0.0014 0.0069 0.0006 0.001 0.0019 0.0015 0.0007 0.0005 0.0013 0.0007 0.0008 0.0013 0.0009 0.0005 0.0016 0.0002
Vacuole 0.0202 0.0151 0.012 0.0116 0.0237 0.009 0.0121 0.011 0.0115 0.0092 0.0065 0.0047 0.0157 0.0155 0.0183 0.0065 0.0109 0.0049
Vacuole Periphery 0.0021 0.0013 0.002 0.0017 0.0021 0.0013 0.0011 0.0011 0.0017 0.0015 0.0009 0.0009 0.0027 0.0007 0.0021 0.0022 0.0027 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.5409 45.1594 37.0376 34.1983 32.749 38.0885 36.8489 41.0904 47.4133 42.7364
Translational Efficiency 1.6192 1.1649 1.3467 1.3787 1.3989 1.6747 1.5507 1.3471 1.1485 1.1677

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
Localization
Cell Percentages ER (93%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Lem3

Lem3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lem3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available