Standard name | |
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Human Ortholog | |
Description | Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 1 | 6 | 20 | 20 | 19 | 22 | 13 | 9 | 0 | 1 | 1 | – | – | – | 1 | 2 | 0 | 2 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 21 | 28 | 33 | 94 | 242 | 424 | 519 | 633 | 521 | 631 | 380 | 585 | 22 | 62 | 87 | – | – | – | 1 | 5 | 5 | 4 | 7 | 9 |
Endoplasmic Reticulum | 0 | 0 | 2 | 1 | 0 | 7 | 3 | 2 | 1 | 0 | 0 | 5 | 3 | 1 | 2 | – | – | – | 1 | 0 | 1 | 0 | 1 | 3 |
Endosome | 10 | 11 | 10 | 27 | 40 | 2 | 6 | 21 | 19 | 17 | 1 | 2 | 14 | 35 | 105 | – | – | – | 8 | 24 | 15 | 19 | 31 | 40 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 9 | 10 | 74 | 76 | 131 | 194 | 298 | 237 | 217 | 206 | 202 | 272 | 7 | 2 | 1 | – | – | – | 1 | 2 | 7 | 6 | 5 | 4 |
Nucleus | 11 | 34 | 8 | 3 | 14 | 20 | 16 | 31 | 13 | 11 | 12 | 14 | 17 | 13 | 15 | – | – | – | 8 | 12 | 3 | 7 | 5 | 5 |
Nuclear Periphery | 3 | 4 | 4 | 15 | 1 | 7 | 13 | 16 | 2 | 8 | 0 | 0 | 0 | 2 | 2 | – | – | – | 1 | 2 | 4 | 4 | 5 | 3 |
Nucleolus | 5 | 2 | 7 | 4 | 2 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | – | – | – | 0 | 0 | 0 | 1 | 2 | 3 |
Vac/Vac Membrane | 129 | 299 | 156 | 263 | 396 | 234 | 312 | 277 | 268 | 286 | 200 | 258 | 147 | 271 | 459 | – | – | – | 143 | 301 | 187 | 254 | 267 | 355 |
Unique Cell Count | 159 | 338 | 228 | 362 | 659 | 729 | 910 | 1032 | 821 | 939 | 613 | 910 | 178 | 341 | 553 | 170 | 356 | 228 | 303 | 337 | 441 | |||
Labelled Cell Count | 189 | 388 | 294 | 486 | 829 | 896 | 1189 | 1242 | 1062 | 1184 | 809 | 1146 | 211 | 387 | 680 | 170 | 356 | 228 | 303 | 337 | 441 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 7.1 | 4.9 | 4.7 | 4.5 | 4.1 | 3.8 | 4.2 | 4.1 | 4.0 | 4.0 | 4.1 | 6.4 | 6.2 | 6.7 | – | – | – | 7.2 | 7.3 | 7.3 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.2 | 1.4 | 1.3 | 1.3 | 1.1 | 1.2 | 1.2 | 0.9 | 1.1 | 1.1 | 1.0 | 1.3 | 1.1 | – | – | – | 1.4 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.12 | -0.25 | -0.37 | -0.21 | -0.24 | -0.29 | -0.28 | -0.26 | 0.37 | 0.33 | 0.44 | – | – | – | 0.55 | 0.56 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 3.3 | 6.3 | 11.5 | 11.5 | 12.8 | 13.1 | 14.4 | 12.3 | 13.5 | -0.6 | 1.2 | 0.4 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 2.5 | 5.2 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -7.1 | -11.0 | -13.9 | – | – | – |
Nucleus | 0 | -1.2 | -0.6 | 0 | -0.4 | 0 | 0 | -1.3 | 0 | 2.5 | 0.2 | -0.6 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 1.1 | -2.2 | -9.7 | -9.4 | -12.0 | -9.7 | -10.6 | -9.3 | -11.2 | 3.3 | 3.0 | 4.5 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5879 | 1.1512 | 0.9274 | 0.5386 | 0.9361 | 0.8234 | 0.7439 | 1.694 | 1.6296 | 1.8995 | 0.6784 | 1.5728 | -1.1541 | 0.1791 | -0.3805 | -0.2115 | -0.9293 | -0.5473 |
Actin | 0.0079 | 0.0006 | 0.0116 | 0.0013 | 0.0114 | 0.0026 | 0.0081 | 0.0002 | 0.0019 | 0.0446 | 0.001 | 0.0042 | 0.0027 | 0 | 0.0009 | 0.0007 | 0.0046 | 0.0003 |
Bud | 0.0013 | 0.0024 | 0.0087 | 0.0017 | 0.0025 | 0.0026 | 0.0004 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0032 | 0.0005 | 0.004 | 0.0006 | 0.0011 | 0.0017 | 0.0009 | 0.0008 | 0.0022 | 0.0005 | 0.0005 | 0.0013 | 0.0006 | 0.0005 | 0.0004 | 0.0007 | 0.0004 | 0.0004 |
Bud Periphery | 0.0012 | 0.0018 | 0.0034 | 0.0048 | 0.0018 | 0.0014 | 0.0006 | 0.0005 | 0.0009 | 0.0008 | 0.0003 | 0.0019 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0002 |
Bud Site | 0.0084 | 0.0048 | 0.0095 | 0.0006 | 0.0022 | 0.0008 | 0.0021 | 0.0017 | 0.0134 | 0.0019 | 0.0007 | 0.0013 | 0.0009 | 0.0015 | 0.0009 | 0.0005 | 0.0002 | 0.0002 |
Cell Periphery | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0009 | 0.0008 | 0.001 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0003 |
Cytoplasm | 0.0444 | 0.0695 | 0.0567 | 0.0287 | 0.0275 | 0.0699 | 0.0451 | 0.0424 | 0.042 | 0.038 | 0.1051 | 0.0615 | 0.0533 | 0.0606 | 0.0685 | 0.033 | 0.0306 | 0.0529 |
Cytoplasmic Foci | 0.0525 | 0.0436 | 0.0449 | 0.0257 | 0.0488 | 0.0465 | 0.0386 | 0.0127 | 0.0389 | 0.0345 | 0.0254 | 0.0296 | 0.0268 | 0.0257 | 0.021 | 0.0233 | 0.0188 | 0.0175 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.006 | 0.004 | 0.004 | 0.0013 | 0.0036 | 0.003 | 0.0078 | 0.0026 | 0.0034 | 0.0029 | 0.0031 | 0.0048 | 0.0094 | 0.0054 | 0.0036 | 0.0021 | 0.0024 | 0.0017 |
Endosome | 0.3397 | 0.2527 | 0.2716 | 0.3119 | 0.3685 | 0.2985 | 0.2978 | 0.2133 | 0.299 | 0.2259 | 0.2009 | 0.3019 | 0.3069 | 0.2311 | 0.2826 | 0.2522 | 0.3344 | 0.2308 |
Golgi | 0.0105 | 0.0025 | 0.0072 | 0.0058 | 0.0209 | 0.0043 | 0.0093 | 0.0022 | 0.0104 | 0.0331 | 0.0098 | 0.0081 | 0.004 | 0.0021 | 0.0043 | 0.0019 | 0.0072 | 0.002 |
Lipid Particles | 0.0329 | 0.0053 | 0.0155 | 0.0109 | 0.0304 | 0.0081 | 0.0209 | 0.0072 | 0.0176 | 0.0121 | 0.024 | 0.01 | 0.0137 | 0.0055 | 0.0125 | 0.0026 | 0.0054 | 0.0072 |
Mitochondria | 0.0208 | 0.0042 | 0.0178 | 0.0064 | 0.0233 | 0.0073 | 0.0089 | 0.0029 | 0.0094 | 0.0201 | 0.0457 | 0.0089 | 0.0031 | 0.0021 | 0.003 | 0.0014 | 0.0029 | 0.0018 |
None | 0.0563 | 0.0715 | 0.1007 | 0.0446 | 0.0472 | 0.0813 | 0.172 | 0.0895 | 0.1189 | 0.0845 | 0.262 | 0.0487 | 0.0975 | 0.0807 | 0.0684 | 0.0904 | 0.0664 | 0.0196 |
Nuclear Periphery | 0.0183 | 0.0088 | 0.0127 | 0.0044 | 0.0062 | 0.0084 | 0.017 | 0.0065 | 0.0054 | 0.0047 | 0.0033 | 0.0079 | 0.023 | 0.0165 | 0.01 | 0.0055 | 0.0123 | 0.0049 |
Nucleolus | 0.0062 | 0.005 | 0.006 | 0.0019 | 0.0041 | 0.0059 | 0.0034 | 0.0037 | 0.0043 | 0.0017 | 0.0011 | 0.0017 | 0.0018 | 0.0019 | 0.0074 | 0.0018 | 0.0007 | 0.0014 |
Nucleus | 0.0154 | 0.0107 | 0.0155 | 0.0046 | 0.008 | 0.018 | 0.0066 | 0.0054 | 0.0071 | 0.0047 | 0.0033 | 0.0048 | 0.0143 | 0.0076 | 0.0103 | 0.0039 | 0.0026 | 0.0067 |
Peroxisomes | 0.0211 | 0.0024 | 0.004 | 0.0129 | 0.0179 | 0.0016 | 0.0123 | 0.0004 | 0.0086 | 0.014 | 0.0038 | 0.0017 | 0.001 | 0.0006 | 0.0061 | 0.0005 | 0.0005 | 0.0083 |
Punctate Nuclear | 0.0122 | 0.0098 | 0.0052 | 0.0018 | 0.0141 | 0.0056 | 0.0018 | 0.0004 | 0.0013 | 0.0013 | 0.0006 | 0.0009 | 0.0047 | 0.0011 | 0.0009 | 0.0019 | 0.001 | 0.0003 |
Vacuole | 0.2924 | 0.4124 | 0.3231 | 0.4366 | 0.3176 | 0.3481 | 0.3003 | 0.4974 | 0.3377 | 0.4139 | 0.2691 | 0.4124 | 0.3481 | 0.4353 | 0.3875 | 0.4841 | 0.4356 | 0.4969 |
Vacuole Periphery | 0.0485 | 0.0871 | 0.0775 | 0.0934 | 0.0423 | 0.0842 | 0.0447 | 0.1089 | 0.076 | 0.0598 | 0.0396 | 0.0869 | 0.0871 | 0.1205 | 0.1107 | 0.0922 | 0.0733 | 0.1465 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.0054 | 9.7564 | 12.3075 | 9.4621 | 10.6128 | 9.8172 | 12.5775 | 13.3161 | 11.1214 | 13.2875 |
Translational Efficiency | 1.0683 | 0.801 | 0.5863 | 0.6181 | 0.6528 | 1.4747 | 0.3898 | 0.4273 | 0.6115 | 0.5737 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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