Standard name
Human Ortholog
Description E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.09 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.99 0.95 0.87 0.79 0.76 0.77 0.71 0.73 0.73 1.0 0.99 0.99 0.99 0.97 0.97 0.93 0.94 0.93 0.9 0.91 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.13 0.26 0.37 0.36 0.4 0.45 0.46 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 0 0 1 0 1 0 0 1 0 0 0 0 1 1 1 0 0 1
Bud 0 1 1 2 3 7 4 8 12 10 9 13 1 1 0 0 1 0 1 1 0 1 0 5
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2 0 1 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 6 3 1 1 9 3 5 9 3 9 11 6 5 14 20 14 17 0 0 0 0 0 0
Cytoplasm 439 322 265 304 405 389 454 437 316 303 238 307 282 400 474 260 177 175 438 305 262 225 254 282
Endoplasmic Reticulum 3 0 4 0 0 4 5 1 1 3 2 5 1 5 4 6 3 6 3 2 4 4 3 16
Endosome 0 0 0 0 2 0 0 0 1 0 0 0 1 0 0 1 0 0 4 3 1 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 0 1
Mitochondria 2 2 6 26 56 116 216 208 166 191 151 202 0 0 0 2 0 1 3 0 4 2 1 5
Nucleus 1 0 1 0 4 2 1 9 2 3 2 0 0 1 2 1 1 2 1 0 0 0 1 3
Nuclear Periphery 0 0 0 0 1 0 3 2 5 1 2 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 0 0 0 1 4 2 6 20 7 25 5 9 0 1 0 0 4 5 5 1 0 8 10 12
Unique Cell Count 443 322 272 308 428 446 577 575 411 424 325 419 283 406 477 263 182 181 472 323 281 251 280 343
Labelled Cell Count 456 331 280 334 477 530 692 693 522 540 419 547 291 414 494 290 200 206 472 323 281 251 280 343


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.6 4.7 4.7 4.9 4.7 4.5 4.9 4.8 4.4 4.8 5.1 7.4 7.5 8.2 8.9 9.1 9.5 8.1 8.2 8.6
Std Deviation (1e-4) 0.8 1.0 0.6 1.2 1.2 1.0 1.2 1.2 1.2 1.1 1.4 1.3 1.4 1.0 1.0 1.5 1.4 1.7 1.5 1.6 1.7
Intensity Change (Log2) – – – -0.01 0.05 0.01 -0.07 0.07 0.02 -0.11 0.03 0.11 0.66 0.67 0.8 0.93 0.96 1.02 0.79 0.8 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.4 0 0 1.6 3.7 3.6 4.2
Cytoplasm 1.1 -1.8 -4.7 -7.1 -7.7 -7.4 -8.6 -8.1 -8.2 2.2 1.0 2.2 1.2 -0.1 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 10.6 0 0 0 12.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.1 0 4.1 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0066 0.0041 0.0038 0.0005 0.0203 0.0043 0.0149 0.0001 0.0209 0.1225 0.0006 0.0094 0.0002 0.0097 0.0002 0.0004 0 0.0005
Bud 0.0016 0.0089 0.0206 0.0003 0.0101 0.0038 0.0005 0.0001 0.0017 0.0144 0.0003 0.0003 0 0.0008 0.0001 0.0001 0 0
Bud Neck 0.0038 0.0015 0.0008 0.0006 0.0008 0.0014 0.0005 0.0001 0.0002 0.0023 0.0064 0.0006 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002
Bud Periphery 0.002 0.0037 0.0056 0.0003 0.0102 0.0034 0.0004 0.0001 0.0021 0.0399 0.0002 0.0005 0.0001 0.0008 0.0001 0.0001 0 0.0001
Bud Site 0.0045 0.0427 0.0107 0.0005 0.0098 0.0009 0.0013 0.0006 0.0032 0.046 0.0077 0.0002 0.0003 0.0091 0.0002 0.0002 0 0.0001
Cell Periphery 0.0005 0.0007 0.0008 0.0001 0.0003 0.0002 0.0004 0.0002 0.0006 0.0053 0.0001 0.0002 0.0017 0.0005 0.0003 0.0002 0 0.0001
Cytoplasm 0.6641 0.7653 0.7533 0.7094 0.6533 0.8117 0.6793 0.8862 0.7544 0.6816 0.7223 0.7873 0.567 0.6695 0.7101 0.8732 0.9905 0.6663
Cytoplasmic Foci 0.0239 0.0097 0.0122 0.0214 0.0188 0.0135 0.012 0.0033 0.0072 0.0241 0.0304 0.0064 0.0032 0.0224 0.0092 0.007 0.0008 0.006
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0.001 0 0 0.0003 0.0003 0 0 0 0.0001
Endoplasmic Reticulum 0.0053 0.0034 0.0046 0.0011 0.0033 0.0042 0.0156 0.002 0.0037 0.0057 0.0055 0.004 0.0062 0.004 0.0018 0.0057 0.0004 0.0186
Endosome 0.0162 0.0053 0.0078 0.0293 0.0092 0.0044 0.0126 0.0017 0.006 0.01 0.0101 0.0026 0.0004 0.0109 0.0024 0.0054 0.0002 0.0258
Golgi 0.0031 0.0007 0.001 0.0129 0.0021 0.0005 0.0065 0.0002 0.0014 0.0054 0.0016 0.0004 0.0001 0.0146 0.0002 0.0004 0 0.0012
Lipid Particles 0.0043 0.0003 0.001 0.0065 0.0013 0.0001 0.0034 0.0001 0.0011 0.0019 0.0004 0.0003 0.0006 0.0009 0.0002 0.0002 0 0.0037
Mitochondria 0.0049 0.0004 0.0032 0.0202 0.0013 0.0009 0.002 0 0.011 0.0043 0.0004 0.0002 0.0001 0.022 0.0001 0.0001 0 0.0031
None 0.2437 0.1409 0.1558 0.1883 0.2454 0.1409 0.2239 0.1035 0.1801 0.0184 0.2 0.1845 0.4181 0.2257 0.2725 0.1017 0.0075 0.26
Nuclear Periphery 0.0014 0.001 0.0017 0.0004 0.0014 0.0008 0.0077 0.0002 0.0007 0.0014 0.0007 0.0004 0.0002 0.0005 0.0003 0.001 0.0001 0.007
Nucleolus 0.0008 0.0004 0.0006 0.0001 0.001 0.0003 0.0002 0 0.0002 0.0002 0.0007 0 0.0001 0.0001 0 0 0 0.0001
Nucleus 0.0022 0.0043 0.0064 0.0011 0.0022 0.0047 0.0087 0.0004 0.0012 0.0011 0.0015 0.0004 0.0002 0.0005 0.0004 0.0006 0.0002 0.0016
Peroxisomes 0.0032 0.001 0.0017 0.0043 0.0037 0.0002 0.0067 0 0.0004 0.0091 0.0049 0.0007 0 0.0056 0 0 0 0.0001
Punctate Nuclear 0.0036 0.0017 0.002 0.0008 0.0024 0.0021 0.0012 0.0001 0.0005 0.0025 0.0041 0.0003 0.0001 0.0002 0.0002 0.0003 0 0.0006
Vacuole 0.0036 0.0036 0.0055 0.0016 0.0026 0.0015 0.0015 0.001 0.0029 0.0024 0.0016 0.0012 0.001 0.0017 0.0016 0.0028 0.0003 0.0033
Vacuole Periphery 0.0006 0.0003 0.0006 0.0004 0.0004 0.0002 0.0007 0.0001 0.0005 0.0005 0.0002 0.0002 0 0.0003 0.0001 0.0003 0 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2785 8.4577 3.9225 7.1719 5.3667 9.4559 10.9567 6.1324 9.9799 8.7396
Translational Efficiency 1.2022 1.2519 2.1005 1.798 1.87 1.5311 1.1999 2.5443 1.6639 1.6329

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg3

Atg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available