Standard name
Human Ortholog
Description Dihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.1 0 0.15 0.21 0.34 0.5 0.4 0.52 0.64 0.71 0.66 0.05 0 0.05 0 0 0 0.05 0 0.1 0.07 0.07 0.08
Nucleus 0.74 0.74 0.78 0.8 0.69 0.68 0.59 0.59 0.57 0.52 0.47 0.45 0.7 0.68 0.68 0.83 0.83 0.87 0.57 0.62 0.38 0.38 0.27 0.19
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.22 0.19 0.08 0.16 0.13 0.1 0.14 0.13 0.1 0.11 0.16 0.24 0.27 0.22 0.07 0.1 0.08 0.35 0.34 0.48 0.48 0.58 0.62
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 2 2 7 8 0 1 8 5 0 0 1 0 0 0 0 0 1 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 6 9 4 2 9 4 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 4 3 1 0 0 0 0 0 0 0 0
Cytoplasm 1 1 5 15 9 4 20 11 7 9 12 11 12 23 23 26 18 11 0 0 0 3 6 5
Endoplasmic Reticulum 0 0 0 1 0 0 0 1 0 1 0 0 0 0 2 7 9 3 0 0 0 2 2 5
Endosome 0 0 0 0 2 0 0 0 0 0 0 1 4 10 16 2 1 1 1 0 3 5 7 10
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 2 1 6 0 1 0 2 0 2 3 12 18
Mitochondria 32 13 10 50 73 172 375 282 252 385 412 455 19 15 28 5 8 4 11 1 29 25 31 43
Nucleus 144 93 197 272 242 344 442 414 276 313 276 309 242 363 381 258 234 209 120 81 110 136 127 104
Nuclear Periphery 2 0 2 1 5 13 23 35 14 15 18 19 1 1 2 4 0 0 0 0 1 2 3 4
Nucleolus 2 2 1 1 1 10 19 18 8 10 19 11 1 0 1 3 0 4 0 0 1 0 1 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 4 7 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 1 0 0 1 1 4 4 0 1 0 0 1 0 3 1 0
Vac/Vac Membrane 38 28 48 29 56 65 73 99 61 63 66 110 82 145 123 21 27 20 73 43 140 172 279 333
Unique Cell Count 194 125 251 342 349 504 747 706 484 604 584 693 348 537 564 311 282 240 213 131 293 358 478 536
Labelled Cell Count 219 137 263 369 392 616 968 875 621 806 815 934 366 572 600 327 300 252 213 131 293 358 478 536


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 8.8 8.4 7.8 7.1 5.9 5.3 5.5 5.0 4.7 4.5 4.6 9.9 9.3 9.0 12.1 12.1 11.2 10.9 11.2 11.5
Std Deviation (1e-4) 1.5 2.1 1.7 1.9 1.8 1.4 1.3 1.3 1.2 1.1 1.3 1.1 6.4 1.8 2.0 3.4 3.6 3.2 2.2 2.3 2.3
Intensity Change (Log2) – – – -0.12 -0.25 -0.51 -0.68 -0.62 -0.76 -0.83 -0.91 -0.88 0.23 0.14 0.09 0.52 0.52 0.41 0.36 0.41 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 0.5 0 0.6 0 0 0 0.1 0 1.1 1.6 1.5 3.3 2.5 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 5.9 9.1 13.0 10.6 12.9 0 0 0 0.8 -0.9 0.6 -1.7 -0.7 -1.5
Nucleus 0.3 -2.5 -2.9 -5.5 -5.6 -5.8 -7.2 -8.3 -9.2 -2.4 -3.1 -3.2 1.3 1.3 2.5
Nuclear Periphery 0 0 0 2.0 2.9 1.8 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.8 -1.0 -2.3 -3.9 -1.9 -2.4 -3.4 -3.0 -1.2 1.3 2.4 0.9 -4.4 -3.2 -3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1019 6.5086 5.9579 5.9946 4.905 5.9779 3.6082 4.7401 4.1081 3.8326 3.1493 3.8243 2.4595 3.3271 2.67 2.0956 1.6465 2.5604
Actin 0.0105 0.0004 0.0146 0.0068 0.0023 0.0008 0.0112 0.0004 0.0064 0.0144 0.0148 0.0025 0.0081 0 0.0156 0.0108 0.0022 0
Bud 0.0005 0.0002 0.0001 0.0006 0.0002 0.0002 0.0007 0.0001 0.0004 0.0003 0.0008 0.0003 0.0004 0.0001 0.0002 0.0004 0.0003 0.0001
Bud Neck 0.0018 0.0002 0.0004 0.0027 0.001 0.0023 0.0017 0.0001 0.0007 0.0003 0.0006 0.003 0.0004 0.0001 0.0003 0.0002 0.0004 0.0007
Bud Periphery 0.0012 0.0005 0.0002 0.0022 0.0004 0.0009 0.0019 0.0001 0.0008 0.0005 0.0029 0.0012 0.0009 0.0002 0.0003 0.0008 0.001 0.0003
Bud Site 0.0013 0.0005 0.0005 0.0008 0.0003 0.0001 0.0037 0.0001 0.0007 0.0007 0.0007 0.0005 0.001 0.0001 0.0007 0.0011 0.0004 0.0001
Cell Periphery 0.0004 0.0001 0.0001 0.0006 0.0002 0.0002 0.0009 0 0.0002 0.0002 0.0007 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0022 0.0011 0.0004 0.0015 0.0105 0.0012 0.0044 0.0016 0.0019 0.0097 0.0005 0.0009 0.0075 0.0008 0.001 0.0009 0.0009 0.0014
Cytoplasmic Foci 0.0021 0.0015 0.0004 0.001 0.004 0.0009 0.0146 0 0.0059 0.0086 0.0007 0.001 0.01 0 0.0066 0.0117 0.0003 0.0001
Eisosomes 0.0002 0 0.0002 0.0003 0 0 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.005 0.0005 0.0009 0.0024 0.0067 0.0005 0.0036 0.0004 0.001 0.0032 0.0008 0.002 0.0033 0.0002 0.0004 0.0016 0.0024 0.0002
Endosome 0.0128 0.0028 0.0015 0.0073 0.0046 0.001 0.0199 0.0003 0.013 0.0221 0.0047 0.0058 0.0333 0.0001 0.0061 0.0317 0.008 0.0021
Golgi 0.0035 0.0004 0.0011 0.0023 0.0013 0.0002 0.0045 0 0.0053 0.0092 0.0072 0.0027 0.0034 0 0.003 0.0173 0.0008 0
Lipid Particles 0.0054 0.0043 0.001 0.011 0.0135 0.0002 0.0091 0 0.0051 0.0104 0.0034 0.0108 0.0098 0 0.0084 0.0054 0.0007 0
Mitochondria 0.0205 0.0084 0.0107 0.0508 0.0213 0.0038 0.021 0.0072 0.0236 0.0364 0.0802 0.0119 0.0189 0.0016 0.0072 0.0538 0.0459 0.0012
None 0.0014 0.0002 0.0003 0.0018 0.0007 0.0002 0.0052 0.0001 0.0005 0.0077 0.0005 0.0003 0.0012 0 0.0005 0.0003 0.0006 0
Nuclear Periphery 0.0275 0.0215 0.0247 0.0233 0.0312 0.0151 0.0353 0.0155 0.0197 0.0066 0.0107 0.0173 0.0167 0.0135 0.0128 0.0152 0.0517 0.0022
Nucleolus 0.0119 0.0069 0.0054 0.0162 0.023 0.0195 0.008 0.0055 0.007 0.004 0.0164 0.0119 0.0073 0.0049 0.0083 0.0073 0.0267 0.0139
Nucleus 0.8723 0.9435 0.934 0.8522 0.867 0.9465 0.8222 0.9666 0.894 0.8566 0.8426 0.9155 0.8553 0.9758 0.9111 0.807 0.8467 0.9691
Peroxisomes 0.003 0.0002 0.0007 0.001 0.0018 0.0001 0.011 0 0.0015 0.0024 0.0012 0.001 0.0008 0 0.0088 0.0237 0.0002 0
Punctate Nuclear 0.0057 0.0016 0.0005 0.0055 0.0053 0.0027 0.0057 0.0002 0.0025 0.0026 0.0004 0.0027 0.0107 0.0001 0.002 0.0014 0.0022 0.0002
Vacuole 0.0054 0.0016 0.0009 0.0038 0.0022 0.0016 0.008 0.0007 0.0043 0.0014 0.0034 0.0045 0.0062 0.0012 0.0034 0.0024 0.0027 0.0044
Vacuole Periphery 0.0052 0.0035 0.0014 0.0059 0.0025 0.0021 0.0073 0.0011 0.0056 0.0024 0.0066 0.0037 0.0042 0.0011 0.0033 0.007 0.0057 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.7218 52.0943 40.5574 32.0996 27.2498 30.5536 33.2441 35.259 29.2045 31.2867
Translational Efficiency 0.8526 0.7466 0.8644 0.9014 1.1285 0.9632 0.8278 0.8444 0.8942 0.8499

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
Localization
Cell Percentages nucleus (46%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Smm1

Smm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available