Standard name
Human Ortholog
Description MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.96 0.95 0.95 0.9 0.89 0.83 0.69 0.75 0.67 0.59 1.0 0.98 0.98 0.96 0.94 0.92 0.9 0.9 0.83 0.9 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.09 0.2 0.09 0.27 0.32 0.32 0.51 0.48 0.6 0.64 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 5
Bud 1 0 10 2 6 5 5 9 6 9 7 12 0 1 4 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 1 5 3 10 5 8 8 5 6 0 3 2 14 20 29 0 0 0 0 0 0
Cytoplasm 146 117 177 124 293 230 390 334 204 249 142 199 67 155 160 392 296 346 150 127 153 93 179 204
Endoplasmic Reticulum 1 1 0 0 2 1 0 3 4 1 0 4 0 1 1 17 9 16 1 1 3 1 5 6
Endosome 2 0 0 0 3 0 1 3 0 1 0 0 0 0 0 5 3 2 3 0 3 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 5 3 0 0 1 0 2
Mitochondria 0 7 16 26 27 69 139 128 150 161 127 217 0 3 5 5 5 7 1 3 11 1 3 4
Nucleus 0 0 2 4 0 1 2 5 1 1 1 1 0 0 0 1 2 2 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 1 2 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 1 0 0 0
Vac/Vac Membrane 4 0 1 1 7 0 4 10 1 12 3 10 0 1 0 12 10 16 2 2 2 1 3 3
Unique Cell Count 154 122 185 130 307 256 437 401 295 332 211 340 67 158 163 408 314 376 168 141 184 104 197 233
Labelled Cell Count 157 126 207 158 343 310 552 501 376 445 287 452 67 164 173 450 348 423 168 141 184 104 197 233


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 4.3 4.2 4.5 4.7 4.2 4.2 4.4 3.6 3.8 3.6 3.6 6.0 6.4 5.5 7.2 7.8 8.0 6.2 6.4 6.9
Std Deviation (1e-4) 1.0 0.6 1.1 1.7 1.2 1.0 1.4 2.1 1.0 1.2 1.1 1.3 0.9 1.2 1.3 1.3 1.7 1.8 1.1 1.0 1.2
Intensity Change (Log2) – – – 0.09 0.17 0.0 -0.0 0.06 -0.22 -0.14 -0.21 -0.23 0.53 0.61 0.41 0.79 0.91 0.94 0.57 0.6 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7025 2.8333 2.1936 2.1429 1.5871 2.4995 1.9881 2.7595 2.5902 2.6341 2.5131 2.5981 2.6841 4.3222 3.3921 3.0279 2.9652 3.3171
Actin 0.0375 0.0003 0.0124 0.0212 0.0321 0.0002 0.0226 0.0005 0.0098 0.0002 0.0154 0.0056 0.113 0.0703 0.0034 0.0022 0.0001 0.0002
Bud 0.0005 0.0003 0.0004 0.0065 0.0003 0.0001 0.0057 0.0139 0.0069 0.0006 0.0028 0.0004 0.0016 0.0005 0.0004 0.0003 0.0001 0
Bud Neck 0.0094 0.0003 0.0034 0.0013 0.0107 0.0009 0.003 0.0002 0.001 0.0003 0.0012 0.0023 0.0041 0.0004 0.0006 0.0004 0.0002 0.0003
Bud Periphery 0.0008 0.0002 0.0005 0.0127 0.0003 0.0001 0.0039 0.0024 0.0065 0.0003 0.0026 0.0007 0.0014 0.0003 0.0002 0.0003 0 0
Bud Site 0.0024 0.0018 0.0029 0.0037 0.0004 0.0002 0.0186 0.0066 0.0076 0.0002 0.0023 0.0002 0.0436 0.0091 0.0071 0.0002 0.0001 0
Cell Periphery 0.0006 0.0011 0.0007 0.0004 0.0002 0.0002 0.0009 0.0003 0.0004 0.0001 0.0005 0.0001 0.0015 0.0002 0.0005 0.0002 0 0.0001
Cytoplasm 0.6496 0.9103 0.7988 0.8493 0.8518 0.8823 0.5187 0.7856 0.715 0.7675 0.594 0.7736 0.4555 0.4916 0.8128 0.7547 0.9856 0.9127
Cytoplasmic Foci 0.0215 0.0074 0.011 0.0138 0.0107 0.0087 0.0409 0.0184 0.0142 0.0153 0.042 0.0175 0.027 0.0131 0.0269 0.0084 0.0032 0.0063
Eisosomes 0.0004 0 0.0002 0.0002 0.0003 0 0.0005 0 0.0001 0 0.0001 0 0.0007 0.0002 0 0 0 0
Endoplasmic Reticulum 0.0195 0.0081 0.0065 0.006 0.0047 0.0068 0.0074 0.0024 0.0022 0.0024 0.0028 0.0021 0.028 0.0052 0.0065 0.0166 0.0015 0.0068
Endosome 0.0212 0.005 0.0091 0.0208 0.0164 0.0077 0.0312 0.0085 0.0107 0.0071 0.0195 0.0086 0.0078 0.008 0.0253 0.0113 0.0029 0.0048
Golgi 0.0051 0.0006 0.003 0.0051 0.006 0.0003 0.0126 0.0009 0.0016 0.0003 0.0074 0.0007 0.0079 0.0021 0.007 0.0006 0.0002 0.0003
Lipid Particles 0.0175 0.0006 0.0086 0.0184 0.0081 0.0008 0.0129 0.0002 0.0018 0.0002 0.01 0.0003 0.0078 0.0009 0.003 0.0003 0 0.0001
Mitochondria 0.0032 0.0003 0.0121 0.0019 0.002 0.0003 0.0084 0.0003 0.0091 0.0004 0.0282 0.0003 0.0071 0.0005 0.0004 0.0002 0.0001 0.0001
None 0.1491 0.0585 0.104 0.0115 0.0435 0.0848 0.2321 0.1559 0.1926 0.1978 0.2186 0.1836 0.2408 0.3857 0.0957 0.1974 0.0045 0.0658
Nuclear Periphery 0.0134 0.0007 0.0012 0.0038 0.0038 0.0015 0.0111 0.0004 0.0013 0.0006 0.0041 0.0005 0.0014 0.0006 0.0011 0.0025 0.0003 0.0004
Nucleolus 0.0014 0.0001 0.001 0.0002 0.0001 0.0001 0.0018 0.0001 0.0003 0.0004 0.0006 0 0.0003 0.0001 0.0001 0 0 0
Nucleus 0.0135 0.001 0.0051 0.002 0.0011 0.0011 0.0095 0.001 0.0031 0.0019 0.0103 0.001 0.0009 0.0008 0.0009 0.0009 0.0004 0.0005
Peroxisomes 0.0081 0.0001 0.0123 0.0099 0.0029 0.0001 0.0363 0.0003 0.01 0.0001 0.0247 0.0003 0.0454 0.0057 0.0012 0.0001 0 0
Punctate Nuclear 0.0161 0.0004 0.0009 0.0038 0.0024 0.001 0.0127 0.0005 0.0008 0.002 0.0057 0.0007 0.0009 0.0009 0.0007 0.0004 0.0001 0.0001
Vacuole 0.0071 0.0028 0.0052 0.0063 0.0019 0.0023 0.0057 0.0015 0.0042 0.0021 0.0062 0.0012 0.0031 0.0035 0.0054 0.0024 0.0006 0.0012
Vacuole Periphery 0.002 0.0002 0.0006 0.0011 0.0006 0.0003 0.0035 0.0002 0.0007 0.0004 0.0007 0.0002 0.0003 0.0004 0.0007 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.3425 15.8153 15.2872 21.3111 22.4854 20.0085 23.1972 24.6387 24.6475 24.7521
Translational Efficiency 0.5144 0.5585 0.4966 0.3717 0.3596 0.4431 0.425 0.3196 0.3342 0.3582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ssk2

Ssk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available