Standard name | |
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Human Ortholog | |
Description | Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family; human homolog DDX51 complements yeast dbp6 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 1 | 2 | 2 | 4 | 0 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 8 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 7 | 1 | 2 | 5 | 8 | 1 | – | 6 | 2 | 5 | 5 | 10 | 19 | 38 | 12 | 19 | 10 | 0 | 1 | 0 | 0 | 3 | 4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2 | 2 | 0 | 7 | 8 | 12 | 28 | – | 4 | 12 | 3 | 6 | 2 | 8 | 16 | 3 | 3 | 0 | 2 | 3 | 0 | 2 | 6 | 10 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 14 | 6 | 1 | 0 | 2 | 6 | 14 | – | 24 | 14 | 34 | 23 | 1 | 1 | 0 | 17 | 6 | 17 | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleus | 72 | 52 | 4 | 6 | 13 | 11 | 32 | – | 13 | 38 | 17 | 28 | 27 | 27 | 27 | 28 | 37 | 42 | 51 | 37 | 8 | 19 | 36 | 46 |
Nuclear Periphery | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 209 | 105 | 13 | 74 | 133 | 110 | 173 | – | 110 | 148 | 107 | 137 | 116 | 221 | 196 | 121 | 125 | 133 | 189 | 109 | 24 | 67 | 210 | 212 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 3 | 0 | – | 1 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 6 | 0 | 0 | 22 | 47 | 42 | 65 | – | 34 | 61 | 30 | 47 | 19 | 40 | 46 | 5 | 3 | 6 | 5 | 1 | 0 | 3 | 20 | 26 |
Vac/Vac Membrane | 2 | 4 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 1 | 0 | 0 | 2 | 3 | 5 | 11 | 8 | 0 | 2 | 0 | 1 | 5 | 18 |
Unique Cell Count | 253 | 154 | 16 | 83 | 148 | 131 | 213 | 136 | 197 | 138 | 181 | 138 | 258 | 268 | 161 | 186 | 185 | 251 | 159 | 42 | 100 | 290 | 339 | |
Labelled Cell Count | 311 | 176 | 19 | 111 | 212 | 192 | 315 | 198 | 288 | 205 | 257 | 175 | 322 | 333 | 194 | 214 | 217 | 251 | 159 | 42 | 100 | 290 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 14.3 | 8.9 | 11.4 | 10.8 | 8.4 | 8.2 | – | 6.4 | 6.8 | 6.1 | 6.4 | 12.6 | 11.8 | 11.2 | 17.8 | 17.2 | 17.1 | 10.7 | 11.9 | 12.2 |
Std Deviation (1e-4) | 2.2 | 3.6 | 2.4 | 2.7 | 2.4 | 2.5 | 1.8 | – | 1.3 | 1.5 | 1.3 | 2.1 | 3.5 | 3.3 | 3.8 | 5.7 | 6.1 | 6.8 | 2.4 | 2.9 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.36 | 0.28 | -0.08 | -0.11 | – | -0.48 | -0.39 | -0.55 | -0.47 | 0.5 | 0.42 | 0.34 | 1.0 | 0.95 | 0.94 | 0.27 | 0.43 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.9 | 1.0 | 0.3 | 0 | – | 0 | -0.5 | -0.3 | -0.5 | 0.3 | 0.5 | -0.7 | -0.5 | -1.2 | -0.8 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6884 | 7.9268 | 6.8986 | 5.1195 | 5.7445 | 6.2216 | 7.3067 | 9.1371 | 7.8553 | 7.5848 | 6.2243 | 7.8942 | 7.1235 | 8.2019 | 6.6115 | 7.2643 | 6.0331 | 6.663 |
Actin | 0.0258 | 0 | 0.012 | 0 | 0.0056 | 0.0057 | 0.0032 | 0 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0273 | 0 | 0.0221 | 0.0614 | 0.0068 | 0.0039 |
Bud | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0007 | 0.0004 | 0.0003 | 0 |
Bud Neck | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.002 | 0.0003 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0005 | 0.0008 | 0.0002 | 0.0022 | 0.0012 | 0.0007 | 0.0005 |
Bud Periphery | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0011 | 0.0004 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0012 | 0.0006 | 0.0006 | 0.0001 |
Bud Site | 0.0025 | 0.0002 | 0.001 | 0.0003 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0059 | 0.0001 | 0.0051 | 0.0012 | 0.0009 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0019 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0028 | 0 | 0.0006 | 0.0064 | 0.0003 | 0.0006 |
Cytoplasmic Foci | 0.0142 | 0 | 0.0012 | 0.0054 | 0.0029 | 0.0048 | 0.0188 | 0.0006 | 0.0109 | 0.0002 | 0.0029 | 0.0006 | 0.0191 | 0.0009 | 0.0026 | 0.0065 | 0.0075 | 0.0013 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0003 | 0 | 0.001 | 0 | 0.0007 | 0.0005 | 0.0005 | 0 | 0.0003 | 0 | 0 | 0 | 0.0055 | 0 | 0.0005 | 0.0042 | 0.0004 | 0.0003 |
Endosome | 0.008 | 0 | 0.0082 | 0.0002 | 0.0089 | 0.0048 | 0.0092 | 0.0004 | 0.0029 | 0 | 0.0002 | 0.0005 | 0.0086 | 0.0001 | 0.0049 | 0.0205 | 0.0067 | 0.0026 |
Golgi | 0.0035 | 0 | 0.0036 | 0.0001 | 0.0051 | 0.006 | 0.0045 | 0.0004 | 0.0019 | 0 | 0.0003 | 0.0005 | 0.0037 | 0 | 0.0022 | 0.0099 | 0.005 | 0.0008 |
Lipid Particles | 0.031 | 0.0001 | 0.004 | 0.0043 | 0.0139 | 0.0074 | 0.0318 | 0.0043 | 0.0082 | 0.0006 | 0.0125 | 0.0062 | 0.0089 | 0.0015 | 0.004 | 0.0042 | 0.0322 | 0.0019 |
Mitochondria | 0.0266 | 0 | 0.013 | 0.001 | 0.0265 | 0.0143 | 0.0072 | 0.0112 | 0.007 | 0.0002 | 0.0072 | 0.0056 | 0.0041 | 0.0001 | 0.0044 | 0.0057 | 0.0056 | 0.0002 |
None | 0.0013 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0016 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0017 | 0 | 0.0007 | 0.0026 | 0.0009 | 0.0069 |
Nuclear Periphery | 0.0005 | 0 | 0.001 | 0.0004 | 0.0049 | 0.0029 | 0.0036 | 0.0007 | 0.0016 | 0.0007 | 0.0009 | 0.0002 | 0.0046 | 0 | 0.0014 | 0.0108 | 0.0033 | 0.002 |
Nucleolus | 0.7677 | 0.8479 | 0.8523 | 0.9065 | 0.8102 | 0.8372 | 0.7631 | 0.8044 | 0.8121 | 0.8962 | 0.8198 | 0.8638 | 0.7502 | 0.8428 | 0.8352 | 0.6529 | 0.7639 | 0.843 |
Nucleus | 0.0914 | 0.1511 | 0.0957 | 0.0725 | 0.0966 | 0.108 | 0.106 | 0.1728 | 0.1385 | 0.0975 | 0.1393 | 0.1122 | 0.1264 | 0.1529 | 0.1057 | 0.1971 | 0.104 | 0.1269 |
Peroxisomes | 0.0067 | 0 | 0.0027 | 0.0003 | 0.001 | 0.0014 | 0.0163 | 0.0002 | 0.0026 | 0 | 0.0012 | 0.0004 | 0.0068 | 0.0001 | 0.0017 | 0.0063 | 0.0114 | 0.0006 |
Punctate Nuclear | 0.0112 | 0.0003 | 0.0006 | 0.0056 | 0.0185 | 0.0019 | 0.0298 | 0.0037 | 0.0098 | 0.003 | 0.013 | 0.0088 | 0.0209 | 0.0005 | 0.0023 | 0.0067 | 0.0482 | 0.0078 |
Vacuole | 0.0043 | 0.0001 | 0.0007 | 0.0013 | 0.0008 | 0.0008 | 0.0008 | 0.0003 | 0.0008 | 0.0002 | 0.0005 | 0.0001 | 0.0005 | 0.0005 | 0.001 | 0.0005 | 0.0005 | 0.0002 |
Vacuole Periphery | 0.0013 | 0 | 0.0004 | 0.0008 | 0.0013 | 0.0009 | 0.0007 | 0.0004 | 0.001 | 0.0003 | 0.001 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0005 | 0.0006 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.4506 | 43.8389 | 30.9813 | 34.2763 | 30.2775 | 19.2551 | 31.3972 | 22.4375 | 21.5654 | 37.7116 |
Translational Efficiency | 1.1599 | 1.0976 | 1.2686 | 0.9639 | 1.3439 | 1.707 | 0.9599 | 1.1688 | 1.1932 | 1.0826 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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