Standard name
Human Ortholog
Description Anchorage subunit of a-agglutinin of a-cells; highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; AGA1 has a paralog, FIG2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.1 0.11 0.12
Bud 0 0 0 0 0 0 0.06 – 0.05 0.06 0.05 0 0 0 0 0 0 0 0.06 0.1 0.05 0.06 0.05 0
Bud Neck 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0
Cytoplasm 0.74 0.76 0.83 0.78 0.65 0.64 0.6 – 0.54 0.55 0.51 0.56 0.91 0.92 0.94 0.95 0.95 0.96 0.39 0.33 0.34 0.05 0 0
Endoplasmic Reticulum 0.09 0.08 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0.09 0.16 0.12 0.08 0.45 0.42 0.42
Endosome 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.18 0.19
Mitochondria 0.09 0 0 0.07 0.14 0.24 0.29 – 0.39 0.39 0.39 0.27 0 0 0 0 0 0 0.19 0.16 0.31 0.09 0.09 0.09
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0.06 – 0.05 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.1 0.07 0.07 – 0.06 0.07 0.11 0.11 0 0 0 0 0 0 0.06 0.08 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 18 20 11 11 6 15 – 10 8 9 7 7 5 1 2 0 0 6 28 17 43 59 69
Bud 4 8 5 7 15 10 31 – 27 31 24 24 10 4 1 0 1 1 14 38 26 24 25 19
Bud Neck 1 1 19 13 9 11 12 – 11 11 2 15 1 2 0 7 3 7 7 13 10 8 6 8
Bud Site 0 0 0 1 0 2 0 – 2 7 6 0 1 0 1 0 0 0 – – – – – –
Cell Periphery 2 8 13 7 4 4 2 – 3 6 4 8 6 1 11 10 3 6 1 5 7 31 25 18
Cytoplasm 146 255 351 270 234 304 306 – 319 304 260 350 607 600 639 527 404 325 90 127 163 21 20 18
Endoplasmic Reticulum 17 28 27 12 9 4 14 – 12 6 3 4 13 14 17 48 18 30 37 45 38 190 234 247
Endosome 7 13 1 8 14 25 15 – 14 4 7 13 12 12 2 1 3 4 6 15 12 3 6 8
Golgi 4 2 4 12 5 7 8 – 1 4 1 3 0 5 4 1 0 0 2 4 2 40 97 110
Mitochondria 18 15 10 25 51 116 146 – 227 215 200 170 16 11 8 24 4 7 44 64 149 40 52 54
Nucleus 0 0 1 1 2 2 4 – 6 4 10 12 0 2 2 7 3 1 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 1 0 – 4 2 2 1 0 0 0 1 0 0 0 1 1 2 1 0
Nucleolus 1 0 0 0 0 4 9 – 8 4 3 5 0 0 1 1 0 0 0 0 0 0 0 1
Peroxisomes 0 3 0 2 5 2 0 – 0 0 0 1 3 2 2 1 0 0 0 0 0 0 0 1
SpindlePole 1 8 2 12 20 17 32 – 28 17 15 54 3 1 2 3 1 2 2 10 13 2 1 2
Vac/Vac Membrane 12 8 10 12 36 34 33 – 38 40 55 67 7 16 11 8 12 2 14 30 30 9 17 20
Unique Cell Count 196 337 421 347 362 476 507 586 551 508 628 665 654 681 556 427 338 233 391 484 423 556 592
Labelled Cell Count 218 367 463 393 415 549 627 710 663 601 734 686 675 702 641 452 385 233 391 484 423 556 592


Ambiguous, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 5.7 5.6 6.5 5.1 5.7 – 4.5 4.4 4.4 4.9 4.7 4.7 4.9 5.5 5.6 5.2 47.1 56.5 67.5
Std Deviation (1e-4) 3.2 3.5 2.1 2.9 4.5 4.3 3.9 – 2.4 2.0 2.2 2.8 2.6 2.5 2.4 1.4 1.3 1.2 19.4 22.8 22.8
Intensity Change (Log2) – – – -0.04 0.18 -0.16 -0.01 – -0.35 -0.39 -0.38 -0.23 -0.27 -0.27 -0.23 -0.06 -0.03 -0.15 3.05 3.31 3.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 -1.2 -3.1 -1.4 – 0 0 0 0 0 0 0 -4.6 -4.6 -4.1
Bud 0.9 2.6 1.1 3.9 – 0 0 0 0 0 0 0 0 0 0
Bud Neck -0.5 -1.5 -1.8 -1.8 – 0 0 0 0 0 0 0 -3.1 -3.5 -1.8
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -1.9 -2.5 -3.2 – 0 0 0 0 0 0 0 -1.3 -2.6 -1.2
Cytoplasm -2.0 -6.0 -6.6 -7.7 – -9.6 -9.3 -10.3 -9.3 3.9 4.2 5.6 5.9 5.2 5.6
Endoplasmic Reticulum -1.9 -2.6 -4.6 -2.7 – 0 0 0 0 0 0 0 1.3 -1.4 1.3
Endosome 0 3.7 4.5 3.2 – 0 0 0 0 0 0 0 0 0 0
Golgi 2.4 0 0.7 0.8 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 6.1 9.5 10.7 – 0 0 0 0 0 0 0 1.6 -1.6 -0.3
Nucleus 0 0 0 0 – 0 0 0 2.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.1 4.3 3.2 4.7 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0.9 4.5 3.3 3.0 – 3.0 3.4 5.0 5.0 -1.7 0.1 -0.9 -1.1 0.4 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.577 2.6487 3.8679 -0.1491 2.3686 2.9202 7.9297 8.8907 6.8316 1.1451 4.3464 6.2312 7.0281 4.3243 5.1812 2.1797 4.5956 4.47
Actin 0.0138 0.0144 0.0126 0.0043 0.0313 0.0165 0.0565 0.0179 0.0459 0.0117 0.017 0.0287 0.0091 0.0144 0.0166 0.0029 0.0315 0.0254
Bud 0.0113 0.0287 0.0217 0.0126 0.0139 0.014 0.0209 0.0376 0.0286 0.045 0.0362 0.0325 0.0149 0.0512 0.0252 0.0255 0.0256 0.0161
Bud Neck 0.0037 0.0026 0.0048 0.0055 0.0084 0.0268 0.0061 0.0036 0.0086 0.0028 0.007 0.0249 0.0054 0.0025 0.0047 0.0078 0.0176 0.0383
Bud Periphery 0.0344 0.049 0.0344 0.1207 0.1442 0.0559 0.0588 0.0491 0.0874 0.1226 0.0573 0.1076 0.0395 0.0593 0.0692 0.0891 0.091 0.0548
Bud Site 0.0275 0.1034 0.0578 0.0062 0.0026 0.0073 0.0186 0.1516 0.0727 0.0128 0.014 0.0078 0.0381 0.1471 0.0637 0.0272 0.0561 0.0043
Cell Periphery 0.0283 0.0188 0.0258 0.0283 0.0107 0.0122 0.0466 0.0445 0.0361 0.0069 0.0207 0.0256 0.0312 0.0169 0.0259 0.0144 0.0213 0.0125
Cytoplasm 0.2643 0.2902 0.2529 0.3751 0.3772 0.2688 0.2833 0.2389 0.3197 0.2343 0.2342 0.288 0.2663 0.2392 0.244 0.3113 0.1706 0.2647
Cytoplasmic Foci 0.0282 0.0146 0.0174 0.015 0.0304 0.0253 0.0296 0.0226 0.0151 0.0178 0.0331 0.0402 0.0333 0.0219 0.0297 0.0278 0.0557 0.0301
Eisosomes 0.0007 0.0006 0.0007 0.0004 0.0006 0.0003 0.0011 0.0012 0.001 0.0003 0.0008 0.0008 0.0006 0.0006 0.0008 0.0001 0.0003 0.0004
Endoplasmic Reticulum 0.0999 0.043 0.126 0.084 0.0345 0.1164 0.1679 0.0836 0.0983 0.1055 0.1398 0.1392 0.138 0.0498 0.0814 0.0643 0.0721 0.0988
Endosome 0.0361 0.0164 0.0333 0.0302 0.0238 0.0311 0.0204 0.0315 0.0118 0.0145 0.0391 0.0301 0.0444 0.0276 0.0422 0.0331 0.0547 0.0576
Golgi 0.0071 0.0047 0.0117 0.0026 0.0073 0.0108 0.0112 0.0079 0.0056 0.0017 0.0214 0.0132 0.0069 0.0049 0.013 0.0035 0.0292 0.0217
Lipid Particles 0.0205 0.0099 0.0104 0.0009 0.0031 0.0128 0.0124 0.0075 0.008 0.0021 0.0095 0.0069 0.0147 0.0068 0.0082 0.0013 0.0161 0.008
Mitochondria 0.0164 0.0113 0.0136 0.0068 0.0099 0.0035 0.0061 0.0095 0.0032 0.0052 0.0073 0.0085 0.0075 0.0079 0.0157 0.0088 0.0165 0.0288
None 0.3572 0.3451 0.3241 0.2431 0.2899 0.3522 0.1821 0.2153 0.2191 0.3582 0.3136 0.1882 0.2497 0.2748 0.287 0.3199 0.2766 0.2814
Nuclear Periphery 0.0079 0.0052 0.0102 0.016 0.0019 0.0115 0.0238 0.0119 0.0079 0.0125 0.0062 0.0116 0.0187 0.0088 0.0117 0.011 0.0094 0.0091
Nucleolus 0.0013 0.0021 0.0013 0.0007 0.0002 0.0006 0.0014 0.0026 0.0012 0.001 0.0011 0.0011 0.0042 0.004 0.0054 0.0014 0.0021 0.0015
Nucleus 0.0035 0.0086 0.0091 0.0091 0.0011 0.0049 0.0072 0.015 0.0061 0.0068 0.0104 0.008 0.0104 0.0183 0.0124 0.0172 0.0172 0.007
Peroxisomes 0.0013 0.0006 0.0019 0.0013 0.0014 0.0005 0.0044 0.0013 0.0013 0.0004 0.0022 0.0007 0.0009 0.0013 0.0014 0.002 0.0027 0.0011
Punctate Nuclear 0.0017 0.0049 0.0031 0.0008 0.0006 0.0015 0.0071 0.0072 0.0019 0.0029 0.0026 0.0046 0.0046 0.0122 0.0049 0.0011 0.009 0.0107
Vacuole 0.0317 0.0245 0.0246 0.0323 0.0056 0.0239 0.0306 0.037 0.0184 0.0301 0.0246 0.0282 0.0572 0.0282 0.0327 0.0268 0.0215 0.0234
Vacuole Periphery 0.0035 0.0015 0.0027 0.0041 0.0012 0.003 0.0039 0.0028 0.002 0.005 0.002 0.0038 0.0044 0.0024 0.0041 0.0035 0.0032 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.5026 12.2278 10.0817 24.1456 20.0338 41.5193 29.6496 24.7246 13.7127 29.4659
Translational Efficiency 1.4752 1.8824 1.4076 0.499 1.5297 1.9669 1.3757 1.4991 2.7012 1.3107

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Anchorage subunit of a-agglutinin of a-cells; highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; AGA1 has a paralog, FIG2, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Aga1

Aga1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aga1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available