ORF
Human Ortholog
Description Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.1 0.11 0.09 0.08 0.17 0.1 0.11 0.14
Bud 0 0 0 0 0 0.05 0.06 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0.05 0.05 0 0.09 0.05 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.08 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.78 0.85 0.79 0.85 0.87 0.78 0.7 0.61 0.64 0.65 0.52 0.58 0.95 0.98 0.95 0.71 0.74 0.69 0.37 0.53 0.29 0.43 0.49 0.49
Endoplasmic Reticulum 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 0.09 0.09 0 0.29 0.4 0.45 0.48 0.43 0.61 0.57 0 0 0 0 0 0 0.22 0.12 0.24 0.12 0.12 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 17 13 6 4 1 3 1 4 4 1 1 3 0 0 31 25 24 12 18 53 20 23 32
Bud 0 8 9 7 5 15 25 12 17 14 12 11 1 0 2 3 8 8 6 11 12 17 11 22
Bud Neck 0 0 1 1 0 0 4 0 0 0 1 1 1 1 1 0 3 0 5 8 16 18 17 18
Bud Site 0 0 1 0 2 8 2 2 1 5 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 5 3 3 1 5 4 0 2 2 2 1 1 1 8 15 12 0 2 6 2 4 5
Cytoplasm 96 173 205 141 156 255 293 189 192 186 122 148 261 214 282 130 187 152 47 127 93 82 108 116
Endoplasmic Reticulum 5 12 14 4 1 3 4 1 9 4 4 5 3 5 2 7 13 12 5 7 19 1 2 5
Endosome 1 2 5 1 5 4 1 4 1 0 2 0 3 1 4 3 8 17 8 10 13 5 5 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 5 1 1 1
Mitochondria 25 3 23 15 7 96 168 140 143 121 144 143 0 0 0 7 10 4 28 27 77 22 26 16
Nucleus 0 0 0 0 1 2 2 0 0 1 2 0 0 0 0 3 2 1 0 0 0 1 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 1 3 1 0 2 0 0 0 0 1 0 6 0 0 3 0 0 1 0 0 0
SpindlePole 0 2 3 4 5 5 6 9 3 2 1 2 4 0 3 7 9 7 0 2 2 4 2 1
Vac/Vac Membrane 0 1 1 0 3 0 2 6 3 2 4 1 1 2 4 7 7 12 9 16 11 10 10 10
Unique Cell Count 123 204 259 166 179 326 420 309 298 284 235 253 274 218 297 182 253 219 128 239 319 193 219 238
Labelled Cell Count 130 219 282 183 195 392 515 370 373 341 298 319 279 224 305 206 287 253 128 239 319 193 219 238


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.7 4.2 4.0 6.2 3.7 3.5 3.4 3.4 3.3 3.0 3.0 5.5 5.6 5.4 7.4 7.1 6.6 5.5 5.5 5.9
Std Deviation (1e-4) 0.7 1.0 0.9 1.5 6.6 1.6 1.4 1.3 1.3 0.8 1.1 0.8 1.1 1.1 1.5 1.0 1.3 1.1 1.3 1.6 1.7
Intensity Change (Log2) – – – -0.07 0.56 -0.17 -0.27 -0.29 -0.29 -0.34 -0.5 -0.48 0.4 0.41 0.37 0.82 0.76 0.65 0.39 0.4 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2907 0.1374 0.2149 -0.2441 -0.6304 0.2292 2.5921 0.1305 0.3917 0.0636 0.1137 0.2087 0.7889 -0.0841 -0.2992 -0.3308 -0.3614 -0.1532
Actin 0.0758 0.0002 0.0045 0.0002 0.0006 0.0143 0.1524 0.0021 0.0087 0.0005 0.0059 0.0084 0.0557 0.0011 0.0117 0.0072 0.0049 0.0158
Bud 0.002 0.0004 0.0011 0 0 0.0002 0.0022 0.0032 0.0014 0.0001 0.0004 0.0003 0.0013 0.0026 0.0044 0.0002 0.0002 0.0017
Bud Neck 0.005 0.0005 0.0004 0 0.0001 0.0269 0.0121 0.0005 0.0029 0.0027 0.0016 0.0141 0.0073 0.0005 0.0016 0.0014 0.0002 0.0227
Bud Periphery 0.0044 0.0005 0.0021 0.0001 0 0.0005 0.003 0.0045 0.0021 0.0002 0.0007 0.0007 0.0017 0.0022 0.0076 0.0002 0.0004 0.0021
Bud Site 0.009 0.0115 0.0069 0 0 0.0004 0.0278 0.0104 0.0373 0.0007 0.0015 0.0003 0.0086 0.0242 0.0225 0.0007 0.0003 0.0021
Cell Periphery 0.0014 0.0003 0.0005 0 0 0.0004 0.0016 0.0004 0.0005 0.0001 0.0001 0.0001 0.0005 0.0003 0.0004 0.0001 0.0001 0.0003
Cytoplasm 0.0339 0.0805 0.0951 0.0895 0.1378 0.1196 0.1115 0.1132 0.1222 0.1292 0.1021 0.1975 0.181 0.1966 0.1851 0.0832 0.218 0.187
Cytoplasmic Foci 0.0419 0.0062 0.0236 0.0037 0.0023 0.0174 0.0982 0.0111 0.0198 0.052 0.0291 0.0078 0.054 0.0208 0.0281 0.0187 0.0109 0.0127
Eisosomes 0.004 0.0002 0.0002 0.0002 0.0002 0.0001 0.0012 0.0001 0.0001 0 0.0001 0 0.0005 0.0001 0.0002 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0107 0.0013 0.005 0.0004 0.0007 0.0022 0.0131 0.0015 0.0021 0.0007 0.0022 0.0007 0.005 0.0014 0.0023 0.0019 0.004 0.0023
Endosome 0.0619 0.0022 0.018 0.0006 0.003 0.0103 0.0825 0.0122 0.0071 0.0223 0.0468 0.0013 0.0553 0.0102 0.0222 0.0113 0.0162 0.0142
Golgi 0.0236 0.0001 0.0023 0.0001 0.0001 0.003 0.032 0.0016 0.0022 0.002 0.0089 0.0005 0.0166 0.0006 0.0028 0.0018 0.0022 0.003
Lipid Particles 0.0608 0.0004 0.0159 0.0007 0.0003 0.0041 0.0323 0.0009 0.0093 0.0074 0.0325 0.0006 0.0264 0.0002 0.007 0.0047 0.0032 0.0034
Mitochondria 0.0421 0.0002 0.0037 0.0004 0.0003 0.0018 0.0136 0.0023 0.0136 0.003 0.0134 0.0003 0.0057 0.0007 0.0034 0.0011 0.0035 0.0017
None 0.4982 0.8898 0.8043 0.9025 0.8122 0.7696 0.2786 0.8302 0.7445 0.7288 0.6507 0.7544 0.5191 0.7266 0.6796 0.8269 0.7013 0.7083
Nuclear Periphery 0.0232 0.0004 0.0011 0.0003 0.0075 0.013 0.0294 0.0005 0.0025 0.0005 0.0085 0.0009 0.0075 0.0005 0.0023 0.0066 0.0237 0.0108
Nucleolus 0.0307 0.0008 0.0024 0.0001 0.002 0.0004 0.006 0.0003 0.0018 0.0005 0.0023 0.0004 0.0013 0.0004 0.0004 0.0022 0.0007 0.0005
Nucleus 0.0089 0.0007 0.0017 0.0002 0.015 0.003 0.0119 0.0008 0.0037 0.0026 0.0027 0.0023 0.003 0.002 0.003 0.0111 0.0029 0.0019
Peroxisomes 0.0247 0.0002 0.0064 0.0003 0.0001 0.0081 0.0438 0.0008 0.0077 0.0414 0.0436 0.0018 0.0264 0.0005 0.0109 0.0048 0.0005 0.0012
Punctate Nuclear 0.0247 0.0028 0.0024 0.0003 0.0168 0.003 0.0371 0.0006 0.009 0.0008 0.0409 0.0063 0.016 0.0015 0.0013 0.0106 0.002 0.0058
Vacuole 0.0089 0.0006 0.002 0.0003 0.0007 0.0008 0.0065 0.0025 0.0013 0.004 0.0035 0.001 0.0053 0.0066 0.0029 0.0043 0.002 0.0017
Vacuole Periphery 0.0041 0.0001 0.0004 0.0001 0.0002 0.001 0.0032 0.0004 0.0003 0.0004 0.0024 0.0002 0.002 0.0004 0.0005 0.0009 0.0026 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.6701 15.021 8.6693 12.4543 12.7085 11.1959 16.4533 10.2507 13.0796 13.4001
Translational Efficiency 0.4022 0.4331 0.7305 0.4117 0.6232 0.7876 0.444 0.6641 0.4834 0.6186

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (44%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

YNR048W

YNR048W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynr048w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available