Standard name
Human Ortholog
Description Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.07 0.08 0.06 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 0.99 0.99 0.98 0.92 0.9 0.83 0.85 0.89 0.81 1.0 0.99 1.0 1.0 0.99 0.99 0.95 0.95 0.96 0.96 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.06 0.12 0.11 0.05 0.13 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 1 0 9 3 3 4 2 6 0 0 0 0 1 1 0 0 1 0 8 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 2 1 12 23 28 33 23 31 41 3 3 1 7 2 3 0 0 2 0 0 0
Cytoplasm 102 131 316 281 351 325 432 382 336 342 270 370 258 337 417 501 506 406 96 111 304 250 454 409
Endoplasmic Reticulum 0 0 0 0 1 4 24 27 47 43 14 61 0 0 0 26 40 20 0 0 1 0 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 2 1 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 0 0 0 2 0 0 0 1 0 2 1 0 1 1 0 0 0 4 1 2
Unique Cell Count 103 131 316 283 353 333 468 425 403 402 302 456 258 339 418 503 510 410 101 117 318 260 472 428
Labelled Cell Count 103 131 321 286 354 342 491 444 419 412 321 481 261 342 419 534 550 432 101 117 318 260 472 428


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 258.6 288.9 254.8 242.4 196.3 158.6 130.5 127.5 114.0 107.1 101.9 101.7 288.1 262.8 231.3 320.8 301.0 294.0 278.0 284.4 287.7
Std Deviation (1e-4) 62.1 64.9 55.6 65.8 46.1 40.2 37.3 35.2 31.0 28.0 27.4 25.4 70.3 63.5 58.7 98.6 80.6 89.2 68.6 76.6 78.5
Intensity Change (Log2) – – – -0.07 -0.38 -0.68 -0.96 -1.0 -1.16 -1.25 -1.32 -1.33 0.18 0.04 -0.14 0.33 0.24 0.21 0.13 0.16 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.9 2.7 3.5 4.2 3.1 4.8 4.5 0 0 0 0 0 0
Cytoplasm -1.5 -1.3 -2.8 -5.0 -5.8 -7.6 -7.2 -5.9 -8.2 0 -1.4 -0.9 -1.1 -1.6 -1.8
Endoplasmic Reticulum 0 0 0 4.1 4.6 6.3 6.0 3.9 6.8 0 0 0 4.1 5.1 4.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 369.594 432.5327 396.3623 374.6013 401.5844 395.6582 393.839 520.8024 449.6817 414.7268 389.5342 456.9202 324.3619 455.8911 406.9597 337.2414 377.0665 443.0327
Actin 0.0006 0.0001 0.0003 0.0004 0.0008 0.0004 0.0003 0.0001 0.0046 0.0003 0.0001 0.0001 0.0008 0.0003 0.0021 0.0006 0.0008 0.0003
Bud 0.0002 0.0001 0.0002 0.0007 0.0004 0.0002 0.0005 0.0001 0.001 0.0008 0.0014 0.0001 0.001 0.0001 0.0009 0.0008 0.0004 0.0001
Bud Neck 0.0004 0.0003 0.0004 0.0006 0.0018 0.0016 0.0003 0.0002 0.0005 0.0006 0.0004 0.0009 0.0002 0.0002 0.0003 0.0005 0.0005 0.0004
Bud Periphery 0.0001 0 0 0.0001 0.0001 0.0001 0.0004 0.0001 0.0005 0.0003 0.0008 0 0.0005 0 0.0004 0.0006 0.0001 0
Bud Site 0 0.0001 0.0001 0.0001 0.0001 0 0.0008 0.0004 0.0002 0.0002 0.0002 0 0.0007 0 0.0003 0.0002 0.0002 0.0001
Cell Periphery 0.0015 0.0011 0.0014 0.0009 0.0005 0.0005 0.0039 0.0022 0.0021 0.0055 0.0033 0.0004 0.0039 0.0007 0.003 0.013 0.0015 0.003
Cytoplasm 0.9579 0.9863 0.9813 0.9479 0.979 0.9591 0.8916 0.9818 0.9439 0.9167 0.9075 0.9832 0.8694 0.9936 0.954 0.8789 0.8916 0.9498
Cytoplasmic Foci 0.0002 0.0001 0.0003 0.0005 0.0006 0.0005 0.0094 0.0039 0.0028 0.0119 0.0006 0.0003 0.0122 0.0001 0.0041 0.0015 0.0037 0.0091
Eisosomes 0 0 0 0 0 0 0.0007 0.0002 0.0003 0.0001 0.0001 0 0.0016 0 0.0002 0.0001 0.0013 0.0002
Endoplasmic Reticulum 0.001 0.0002 0.0003 0.0003 0.0006 0.001 0.001 0.0002 0.0008 0.0007 0.0003 0.0005 0.0014 0.0001 0.0003 0.0011 0.0012 0.0011
Endosome 0.0001 0 0.0001 0.0002 0.0003 0.0003 0.0021 0.0001 0.0006 0.0007 0.0002 0.0001 0.0009 0 0.0006 0.0016 0.0027 0.0002
Golgi 0 0 0 0 0 0 0.0009 0 0.0007 0.0003 0 0 0.0002 0 0.0002 0.0001 0.0001 0.0001
Lipid Particles 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0072 0.0003 0.0007 0.0046 0.0001 0.0001 0.003 0 0.0004 0.0008 0.0015 0.0019
Mitochondria 0.0008 0.0002 0.0004 0.0011 0.0003 0.0005 0.0065 0.0012 0.0052 0.0044 0.0075 0.0001 0.0094 0.001 0.0082 0.005 0.0039 0.0015
None 0.0012 0.0002 0.0006 0.0007 0.0003 0.0008 0.0061 0.0012 0.0025 0.0141 0.0027 0.0003 0.0238 0.0002 0.0068 0.0073 0.0168 0.0075
Nuclear Periphery 0.014 0.0021 0.0037 0.0106 0.0039 0.014 0.0154 0.0012 0.0046 0.0052 0.0037 0.0041 0.014 0.0011 0.0037 0.0155 0.0267 0.0065
Nucleolus 0.0006 0.0001 0.0006 0.001 0.0001 0.0002 0.0253 0.0018 0.0067 0.0039 0.0173 0.0001 0.017 0 0.0036 0.0067 0.0061 0.0037
Nucleus 0.0171 0.006 0.0084 0.0232 0.0084 0.0158 0.0151 0.0028 0.0141 0.0155 0.0273 0.0074 0.0221 0.0018 0.0054 0.0223 0.0159 0.009
Peroxisomes 0 0 0 0.0001 0 0 0.0012 0.0001 0.0002 0.0003 0.0002 0 0.0006 0 0.0003 0.0001 0.0003 0.0001
Punctate Nuclear 0.0001 0 0.0001 0.0003 0.0001 0.0002 0.0009 0.0005 0.0004 0.0011 0.0001 0.0001 0.001 0 0.0002 0.0006 0.0006 0.0002
Vacuole 0.0029 0.0023 0.0014 0.0074 0.002 0.0031 0.008 0.0015 0.0046 0.0101 0.0165 0.0017 0.0097 0.0005 0.003 0.0262 0.0145 0.0038
Vacuole Periphery 0.0011 0.0007 0.0004 0.0038 0.0006 0.0013 0.0023 0.0002 0.0028 0.0028 0.0097 0.0006 0.0062 0.0001 0.002 0.0165 0.0095 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 602.3058 559.7556 575.8315 666.0859 567.5508 762.3338 1025.7711 985.5956 903.57 681.3772
Translational Efficiency 1.6784 1.9226 1.8383 1.4539 1.6927 2.0581 1.7709 1.6108 1.5456 1.5953

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Lys9

Lys9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available