Standard name | |
---|---|
Human Ortholog | |
Description | Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | – | 4 | 4 | 5 | 5 | 10 | 4 | 6 | 4 | 4 | 10 | 20 | 29 | 16 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 1 | 5 | 3 | 2 | 1 | 1 | 0 | 4 | 1 | 3 | 6 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | – | 0 | 0 | 4 | 5 | 1 | 8 | 4 | 21 | 6 | 0 | 4 | 2 | 5 | 5 | 6 | 0 | 1 | 1 | 0 | 1 | 0 |
Nucleus | 361 | 95 | – | 10 | 24 | 49 | 113 | 113 | 80 | 133 | 109 | 128 | 131 | 103 | 61 | 231 | 232 | 245 | 358 | 102 | 35 | 65 | 162 | 165 |
Nuclear Periphery | 0 | 0 | – | 0 | 1 | 3 | 2 | 4 | 4 | 4 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 1 | – | 8 | 53 | 35 | 39 | 28 | 40 | 30 | 43 | 38 | 32 | 85 | 178 | 15 | 23 | 17 | 6 | 2 | 1 | 1 | 23 | 25 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 4 | 5 | – | 0 | 5 | 3 | 10 | 6 | 5 | 1 | 3 | 7 | 4 | 2 | 5 | 12 | 11 | 10 | 3 | 3 | 1 | 0 | 28 | 60 |
Unique Cell Count | 369 | 101 | 22 | 84 | 90 | 166 | 150 | 124 | 165 | 157 | 173 | 170 | 204 | 271 | 270 | 269 | 284 | 372 | 113 | 44 | 70 | 223 | 264 | |
Labelled Cell Count | 370 | 104 | 22 | 91 | 104 | 180 | 164 | 145 | 180 | 187 | 196 | 178 | 222 | 294 | 280 | 284 | 293 | 372 | 113 | 44 | 70 | 223 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.3 | 32.0 | – | 21.9 | 19.6 | 11.4 | 10.4 | 9.5 | 7.8 | 8.5 | 7.6 | 8.7 | 26.4 | 22.6 | 20.5 | 31.1 | 31.0 | 33.3 | 25.7 | 23.9 | 24.0 |
Std Deviation (1e-4) | 5.0 | 9.4 | – | 8.9 | 6.8 | 2.9 | 3.1 | 2.8 | 2.0 | 2.5 | 1.9 | 2.9 | 9.4 | 8.5 | 6.9 | 13.1 | 11.8 | 13.7 | 8.2 | 6.7 | 7.8 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.0345 | 31.5558 | 27.2758 | 29.3518 | 28.434 | 29.4184 | 18.0288 | 41.3028 | 36.8607 | 32.3179 | 33.1027 | 35.0711 | 19.6303 | 33.8297 | 29.7295 | 28.3566 | 26.9116 | 27.4401 |
Actin | 0.0837 | 0.0043 | 0.0115 | 0.0014 | 0.0484 | 0.0001 | 0.0192 | 0.0001 | 0.0048 | 0 | 0.0002 | 0.0001 | 0.0287 | 0 | 0.0001 | 0 | 0.0191 | 0.0054 |
Bud | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0032 | 0.0001 | 0.0006 | 0.0004 | 0.0012 | 0.0002 | 0.0004 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0007 | 0.0002 | 0.0041 | 0.0001 | 0.0003 | 0.0009 | 0.002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0009 |
Bud Periphery | 0.0014 | 0.0003 | 0.0005 | 0.0004 | 0.0007 | 0.001 | 0.0033 | 0.0001 | 0.0009 | 0.0008 | 0.002 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Bud Site | 0.0052 | 0.0012 | 0.0008 | 0.0002 | 0.0004 | 0.0003 | 0.0086 | 0.005 | 0.0038 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 |
Cell Periphery | 0.0014 | 0.001 | 0.0008 | 0.0001 | 0.0002 | 0.002 | 0.0027 | 0.0009 | 0.001 | 0.003 | 0.0012 | 0.002 | 0.0009 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.001 |
Cytoplasm | 0.0166 | 0.0005 | 0.0029 | 0.0001 | 0.0007 | 0.0001 | 0.0121 | 0.0001 | 0.0006 | 0.0003 | 0.0014 | 0.0003 | 0.0043 | 0.0001 | 0.0033 | 0.0001 | 0.0002 | 0.0002 |
Cytoplasmic Foci | 0.0367 | 0.0032 | 0.0149 | 0.0006 | 0.0021 | 0.0001 | 0.1046 | 0 | 0.0053 | 0.0025 | 0.0002 | 0.001 | 0.0174 | 0 | 0.0059 | 0.0002 | 0.0015 | 0.004 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0006 | 0.0019 | 0.0001 | 0.0014 | 0 | 0.0054 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0013 | 0 | 0 | 0 | 0.0004 | 0.0002 |
Endosome | 0.0356 | 0.0009 | 0.0108 | 0.0031 | 0.0217 | 0.0001 | 0.0437 | 0 | 0.0032 | 0 | 0.0001 | 0.003 | 0.0051 | 0 | 0.0009 | 0 | 0.0034 | 0.0013 |
Golgi | 0.0058 | 0.0003 | 0.0053 | 0.0024 | 0.006 | 0 | 0.0102 | 0 | 0.004 | 0 | 0 | 0.0021 | 0.0031 | 0 | 0.0004 | 0 | 0.0023 | 0.001 |
Lipid Particles | 0.0175 | 0.0003 | 0.0065 | 0.0027 | 0.0013 | 0.0004 | 0.0643 | 0 | 0.0014 | 0.0014 | 0.0002 | 0.0007 | 0.0069 | 0 | 0.0018 | 0.0001 | 0.0074 | 0.0021 |
Mitochondria | 0.0135 | 0.0025 | 0.0135 | 0.0391 | 0.014 | 0.0011 | 0.0078 | 0.0009 | 0.0041 | 0.0033 | 0.0201 | 0.0076 | 0.0138 | 0.0006 | 0.0009 | 0.0004 | 0.0011 | 0.0013 |
None | 0.0173 | 0.0002 | 0.0022 | 0.0001 | 0.0003 | 0 | 0.0131 | 0 | 0.0004 | 0.0004 | 0.0006 | 0.0001 | 0.0138 | 0 | 0.0002 | 0.0001 | 0.0013 | 0.0002 |
Nuclear Periphery | 0.0237 | 0.008 | 0.0057 | 0.0023 | 0.0045 | 0.0008 | 0.052 | 0.0061 | 0.0054 | 0.0006 | 0.0027 | 0.0026 | 0.0034 | 0.007 | 0.0027 | 0.002 | 0.0057 | 0.0062 |
Nucleolus | 0.1627 | 0.0893 | 0.1842 | 0.1173 | 0.183 | 0.1706 | 0.0698 | 0.1101 | 0.2746 | 0.1363 | 0.2522 | 0.2672 | 0.2605 | 0.1039 | 0.2139 | 0.1269 | 0.1831 | 0.1947 |
Nucleus | 0.4956 | 0.877 | 0.7201 | 0.8262 | 0.7082 | 0.8121 | 0.5086 | 0.8724 | 0.6764 | 0.8387 | 0.6962 | 0.7005 | 0.601 | 0.8825 | 0.7623 | 0.8511 | 0.7638 | 0.7689 |
Peroxisomes | 0.0076 | 0.0002 | 0.0046 | 0.0014 | 0.0017 | 0.0001 | 0.0343 | 0 | 0.0017 | 0.0001 | 0.0005 | 0.0011 | 0.0124 | 0 | 0.0032 | 0 | 0.0027 | 0.0012 |
Punctate Nuclear | 0.0432 | 0.0033 | 0.0041 | 0.0002 | 0.0004 | 0.0001 | 0.0177 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0166 | 0.0001 | 0.0011 | 0.0165 | 0.0067 | 0.006 |
Vacuole | 0.0115 | 0.0022 | 0.0035 | 0.0007 | 0.0013 | 0.0047 | 0.0112 | 0.0019 | 0.0024 | 0.0058 | 0.0045 | 0.0047 | 0.0022 | 0.0019 | 0.001 | 0.0009 | 0.0002 | 0.0025 |
Vacuole Periphery | 0.0115 | 0.0042 | 0.0053 | 0.0013 | 0.0026 | 0.0053 | 0.0074 | 0.0021 | 0.0039 | 0.006 | 0.016 | 0.0051 | 0.0029 | 0.0028 | 0.001 | 0.0013 | 0.0005 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 112.153 | 114.0983 | 122.2394 | 84.7466 | 110.6497 | 73.9725 | 80.8918 | 54.5965 | 60.4851 | 95.0003 |
Translational Efficiency | 1.2348 | 1.1728 | 0.9485 | 1.046 | 1.0844 | 1.2031 | 1.0711 | 1.2538 | 0.8457 | 1.0512 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|