Standard name | |
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Human Ortholog | |
Description | Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 51 | 12 | 53 | 33 | 19 | 9 | 5 | 20 | 11 | 19 | 38 | 11 | 21 | 9 | 12 | – | – | 8 | 14 | 10 | 25 | 22 | 36 | 36 |
Bud | 14 | 2 | 12 | 18 | 11 | 20 | 20 | 36 | 28 | 32 | 21 | 29 | 5 | 14 | 5 | – | – | 11 | 1 | 1 | 2 | 1 | 4 | 3 |
Bud Neck | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 6 | 1 | 5 | – | – | 1 | 8 | 9 | 4 | 15 | 23 | 30 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 113 | 54 | 187 | 143 | 99 | 142 | 187 | 139 | 122 | 167 | 142 | 152 | 143 | 141 | 173 | – | – | 36 | 186 | 86 | 295 | 243 | 433 | 425 |
Cytoplasm | 80 | 33 | 116 | 129 | 138 | 258 | 308 | 358 | 224 | 265 | 171 | 179 | 232 | 230 | 247 | – | – | 33 | 16 | 5 | 8 | 19 | 19 | 19 |
Endoplasmic Reticulum | 4 | 2 | 2 | 1 | 2 | 5 | 6 | 5 | 4 | 3 | 18 | 5 | 2 | 3 | 3 | – | – | 4 | 15 | 0 | 14 | 6 | 19 | 17 |
Endosome | 1 | 0 | 2 | 0 | 4 | 3 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | 0 | 4 | 0 | 1 | 2 | 3 | 3 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 2 | 1 | 6 | 0 |
Mitochondria | 20 | 4 | 63 | 89 | 44 | 191 | 264 | 321 | 245 | 309 | 274 | 253 | 0 | 2 | 0 | – | – | 11 | 5 | 1 | 9 | 19 | 19 | 18 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 2 | 3 | 1 | 0 | 2 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 35 | 33 | 20 | 22 | 19 | 20 | 6 | 5 | 5 | 9 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 4 | 0 | 7 | 5 | 5 | 3 | 7 | 10 | 3 | 1 | 2 | 6 | 2 | 1 | 0 | – | – | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 6 | 3 | 7 | 2 | 7 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | 3 | 2 | 0 | 2 | 6 | 8 |
Unique Cell Count | 210 | 80 | 311 | 285 | 260 | 462 | 589 | 700 | 457 | 551 | 460 | 450 | 319 | 308 | 334 | 72 | 263 | 119 | 369 | 337 | 579 | 572 | ||
Labelled Cell Count | 291 | 108 | 479 | 458 | 346 | 660 | 825 | 925 | 648 | 806 | 678 | 649 | 411 | 404 | 445 | 106 | 263 | 119 | 369 | 337 | 579 | 572 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 6.2 | 6.0 | 5.8 | 5.6 | 4.7 | 4.6 | 4.8 | 4.7 | 4.4 | 4.6 | 4.5 | 6.3 | 6.5 | 6.8 | 4.5 | 4.7 | 5.7 | 6.6 | 6.8 | 6.8 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.7 | 1.7 | 1.6 | 1.3 | 1.4 | 1.3 | 1.3 | 0.9 | 1.2 | 1.0 | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 | 0.9 | 1.1 | 1.9 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.09 | -0.33 | -0.37 | -0.3 | -0.34 | -0.45 | -0.36 | -0.4 | 0.09 | 0.12 | 0.19 | -0.42 | -0.34 | -0.07 | 0.14 | 0.2 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.9 | -3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.1 | -5.9 | -5.7 | – | – | -1.2 |
Bud | 1.4 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | 0.4 | -1.9 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | -2.4 | -5.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.8 | -3.6 | -2.1 | – | – | -1.6 |
Cytoplasm | 2.0 | 3.8 | 5.1 | 4.3 | 4.1 | 3.2 | 3.1 | 0 | 0.7 | 8.9 | 9.4 | 9.4 | – | – | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Mitochondria | 3.1 | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -8.5 | -8.0 | -8.7 | – | – | -1.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Peroxisomes | 0.1 | -1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.2 | -6.1 | -6.3 | – | – | -3.0 |
SpindlePole | -0.4 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5925 | 2.8208 | 2.9613 | 2.2617 | 2.4351 | 2.5165 | 2.5706 | 3.6513 | 3.0752 | 3.1652 | 3.8328 | 3.5082 | 3.1913 | 3.5449 | 4.2997 | 3.8103 | 3.5199 | 4.3976 |
Actin | 0.061 | 0.0056 | 0.0058 | 0.0337 | 0.0083 | 0.0016 | 0.07 | 0.0058 | 0.0531 | 0.0101 | 0.0004 | 0.0266 | 0.0002 | 0.0002 | 0.0256 | 0.0004 | 0.0031 | 0.0014 |
Bud | 0.0016 | 0.0002 | 0.0004 | 0.0015 | 0.0019 | 0.0003 | 0.0024 | 0.0003 | 0.001 | 0.0005 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0004 | 0.0008 | 0.0003 | 0.0019 |
Bud Neck | 0.0049 | 0.0032 | 0.0052 | 0.0385 | 0.0031 | 0.0033 | 0.0075 | 0.0052 | 0.0047 | 0.005 | 0.0016 | 0.0147 | 0.0011 | 0.0014 | 0.0062 | 0.002 | 0.0028 | 0.0091 |
Bud Periphery | 0.0078 | 0.0016 | 0.0036 | 0.0104 | 0.0067 | 0.0061 | 0.0132 | 0.0015 | 0.0057 | 0.0091 | 0.002 | 0.0093 | 0.0011 | 0.0005 | 0.0025 | 0.0224 | 0.0038 | 0.0114 |
Bud Site | 0.0153 | 0.0439 | 0.0402 | 0.0321 | 0.0057 | 0.0009 | 0.0758 | 0.1331 | 0.0138 | 0.0034 | 0.0007 | 0.0119 | 0.0099 | 0.0604 | 0.013 | 0.0015 | 0.0024 | 0.0041 |
Cell Periphery | 0.3035 | 0.3082 | 0.2932 | 0.2554 | 0.2885 | 0.3881 | 0.3715 | 0.2799 | 0.3715 | 0.4974 | 0.3783 | 0.3737 | 0.4248 | 0.5083 | 0.3819 | 0.8287 | 0.4115 | 0.4847 |
Cytoplasm | 0.3026 | 0.5544 | 0.4841 | 0.4567 | 0.2667 | 0.5065 | 0.3085 | 0.4697 | 0.4453 | 0.3636 | 0.4342 | 0.416 | 0.491 | 0.4186 | 0.437 | 0.1397 | 0.4137 | 0.3936 |
Cytoplasmic Foci | 0.039 | 0.0015 | 0.0044 | 0.0234 | 0.0639 | 0.0024 | 0.0033 | 0.0017 | 0.0049 | 0.0006 | 0.0008 | 0.0117 | 0.0018 | 0.0001 | 0.0017 | 0 | 0.0105 | 0.0012 |
Eisosomes | 0.0038 | 0.0035 | 0.0031 | 0.0015 | 0.0009 | 0.0011 | 0.0073 | 0.0038 | 0.0015 | 0.0019 | 0.0003 | 0.0024 | 0.001 | 0.0012 | 0.0032 | 0.0001 | 0.0006 | 0.0004 |
Endoplasmic Reticulum | 0.0138 | 0.0089 | 0.008 | 0.0053 | 0.0108 | 0.0124 | 0.0184 | 0.0037 | 0.004 | 0.0066 | 0.0033 | 0.0055 | 0.0073 | 0.006 | 0.0056 | 0.0014 | 0.0109 | 0.0023 |
Endosome | 0.0366 | 0.0002 | 0.0007 | 0.0277 | 0.1494 | 0.0019 | 0.0036 | 0.0001 | 0.0061 | 0 | 0.0003 | 0.0029 | 0.0001 | 0 | 0.0067 | 0 | 0.0311 | 0.0018 |
Golgi | 0.017 | 0.001 | 0.002 | 0.005 | 0.0873 | 0.002 | 0.0046 | 0.0003 | 0.0081 | 0.0003 | 0.0007 | 0.0049 | 0.0001 | 0.0001 | 0.0016 | 0 | 0.0289 | 0.0005 |
Lipid Particles | 0.0259 | 0.0016 | 0.0027 | 0.0038 | 0.0248 | 0.0025 | 0.0039 | 0.001 | 0.0024 | 0.0007 | 0.0032 | 0.0066 | 0.0039 | 0.0004 | 0.0134 | 0.0002 | 0.0141 | 0.0015 |
Mitochondria | 0.0223 | 0.0001 | 0.0004 | 0.0013 | 0.0142 | 0.0092 | 0.002 | 0 | 0.0184 | 0 | 0.0754 | 0.0157 | 0 | 0 | 0.0009 | 0 | 0.0073 | 0.0002 |
None | 0.0875 | 0.0631 | 0.1338 | 0.0829 | 0.0017 | 0.0501 | 0.0986 | 0.0912 | 0.0539 | 0.0988 | 0.0928 | 0.0829 | 0.0503 | 0.0017 | 0.0537 | 0.0002 | 0.0513 | 0.0686 |
Nuclear Periphery | 0.0174 | 0.0003 | 0.0002 | 0.0007 | 0.0022 | 0.0012 | 0.0014 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0101 | 0.0001 | 0.0004 | 0.0002 |
Nucleolus | 0.001 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0009 | 0 | 0.0001 | 0.0003 |
Nucleus | 0.003 | 0.0003 | 0.0003 | 0.0014 | 0.0021 | 0.0021 | 0.0008 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0183 | 0.0001 | 0.0003 | 0.0003 |
Peroxisomes | 0.0151 | 0 | 0.0094 | 0.0035 | 0.026 | 0.0047 | 0.0008 | 0.0001 | 0.0016 | 0 | 0.0036 | 0.0076 | 0 | 0 | 0.0006 | 0 | 0.0028 | 0.0001 |
Punctate Nuclear | 0.0095 | 0.0001 | 0.0002 | 0.0035 | 0.0008 | 0.0003 | 0.0021 | 0.0001 | 0.0002 | 0 | 0 | 0.0007 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 |
Vacuole | 0.0095 | 0.0023 | 0.0021 | 0.0105 | 0.0314 | 0.0027 | 0.0033 | 0.0015 | 0.0026 | 0.0014 | 0.0018 | 0.0038 | 0.0062 | 0.0006 | 0.0146 | 0.0022 | 0.0032 | 0.0161 |
Vacuole Periphery | 0.0019 | 0 | 0 | 0.0007 | 0.0035 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0015 | 0 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4714 | 17.3638 | 25.7694 | 27.4989 | 23.5592 | 17.5345 | 29.1665 | 33.8283 | 29.2307 | 26.2832 |
Translational Efficiency | 0.5299 | 0.6534 | 0.4599 | 0.4714 | 0.4189 | 0.3811 | 0.3929 | 0.311 | 0.3072 | 0.306 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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