Standard name
Human Ortholog
Description Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.09 0.06 0 0.05 0 0 0
Cytoplasm 0.93 0.95 0.91 0.93 0.9 0.89 0.78 0.77 0.65 0.61 0.56 0.56 0.99 0.99 0.99 0.69 0.68 0.67 0.71 0.76 0.35 0.75 0.79 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.16 0.25 0.26 0.46 0.56 0.58 0.53 0 0 0 0.14 0.05 0.07 0.09 0.1 0.2 0.16 0.09 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.21 0.15 0 0 0.17 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 3 3 2 0 2 2 0 0 0 1 0 1 1 2 1 4 6 3 16 2 4 4
Bud 5 3 5 4 0 5 2 15 17 12 24 10 0 1 0 1 2 2 1 5 9 0 1 6
Bud Neck 1 0 1 1 0 1 0 3 2 1 1 1 12 13 8 0 1 0 4 2 7 1 0 6
Bud Site 0 0 0 1 0 1 0 1 1 3 0 0 0 0 1 1 0 0 – – – – – –
Cell Periphery 5 2 10 11 4 7 4 1 1 2 4 5 17 9 6 21 5 10 9 5 13 2 2 1
Cytoplasm 109 208 249 240 221 383 365 527 298 317 281 287 441 467 475 122 56 72 99 166 96 101 141 140
Endoplasmic Reticulum 1 1 4 0 0 0 0 3 1 0 1 1 3 3 2 4 7 6 1 1 10 0 0 0
Endosome 1 0 2 2 5 5 10 14 7 4 2 6 0 0 0 4 3 1 1 1 7 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 0
Mitochondria 3 11 7 7 6 71 117 177 210 291 287 272 0 0 0 25 4 8 13 22 55 21 16 15
Nucleus 0 0 0 1 0 2 2 5 0 1 2 1 1 1 1 10 3 6 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 1 1 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 0 1 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 1 2 5 2 9 6 0 1 4 6 0 0 0 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 7 17 16 17 9 12 19 0 0 1 0 1 0 1 2 2 0 2 3
Vac/Vac Membrane 0 0 6 1 10 9 10 8 11 4 4 12 0 0 0 32 17 16 1 5 45 2 5 6
Unique Cell Count 117 220 275 257 245 432 469 686 455 521 499 512 446 472 479 178 82 108 141 221 272 135 179 192
Labelled Cell Count 128 229 290 275 254 494 539 780 566 646 625 624 475 495 495 223 101 126 141 221 272 135 179 192


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 4.4 4.3 4.0 3.4 3.0 3.4 3.0 2.6 2.9 2.9 4.5 4.9 4.9 6.3 6.1 6.7 4.7 4.9 5.2
Std Deviation (1e-4) 2.1 1.7 2.6 2.4 1.9 2.0 1.3 1.9 1.3 0.8 1.6 0.8 0.7 1.1 0.8 5.8 5.9 5.3 0.7 1.2 1.3
Intensity Change (Log2) – – – -0.04 -0.14 -0.38 -0.57 -0.38 -0.59 -0.79 -0.63 -0.63 0.03 0.13 0.15 0.49 0.47 0.6 0.08 0.14 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.9 6.0 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2571 0.2598 0.0353 0.2372 -0.2257 0.5599 1.9661 2.6208 1.0338 0.7779 0.1134 1.518 0.3694 0.3212 0.3852 0.9224 0.0218 0.6187
Actin 0.0337 0.0006 0.0056 0.007 0.0011 0.0229 0.1338 0.0014 0.0005 0.0001 0.0007 0.0065 0.0674 0.0002 0.0028 0.0372 0 0.0025
Bud 0.0012 0.0075 0.0012 0.0015 0.0011 0.0019 0.0059 0.0085 0.0004 0 0.0217 0.0005 0.0002 0.0007 0.0012 0.002 0 0.0016
Bud Neck 0.0074 0.0003 0.0022 0.0006 0.0003 0.017 0.0014 0.0008 0.0024 0.0001 0.0013 0.0019 0.0002 0.0001 0.0002 0.0016 0 0.0004
Bud Periphery 0.0069 0.012 0.01 0.0021 0.0019 0.0113 0.0381 0.0625 0.0008 0 0.0345 0.004 0.0005 0.0015 0.0019 0.0043 0.0001 0.0143
Bud Site 0.0064 0.0061 0.006 0.0013 0.0006 0.0017 0.0139 0.0339 0.014 0.0001 0.0042 0.0017 0.0004 0.0156 0.0102 0.0209 0 0.0008
Cell Periphery 0.0041 0.0089 0.0013 0.0012 0.0003 0.0037 0.0153 0.068 0.0061 0.0001 0.0011 0.0381 0.0015 0.0011 0.0015 0.0026 0.0001 0.0104
Cytoplasm 0.0294 0.047 0.0523 0.0789 0.0323 0.0668 0.0858 0.0976 0.085 0.1164 0.2706 0.1218 0.146 0.1576 0.0924 0.1566 0.1078 0.1394
Cytoplasmic Foci 0.0192 0.0106 0.0162 0.0222 0.0084 0.0243 0.0373 0.0045 0.0107 0.0024 0.0422 0.004 0.0289 0.0116 0.0258 0.0086 0.0196 0.0199
Eisosomes 0.0011 0.0002 0.0004 0.0002 0.0007 0.0003 0.0048 0.0011 0.0005 0.0001 0.0001 0.0002 0.0012 0.0001 0.0004 0.0012 0.0001 0.0001
Endoplasmic Reticulum 0.0075 0.0013 0.0013 0.0032 0.0009 0.001 0.0526 0.0017 0.0007 0.0005 0.0427 0.0011 0.0083 0.0035 0.0041 0.0169 0.0013 0.0022
Endosome 0.0258 0.0137 0.0089 0.0431 0.0195 0.0214 0.0386 0.0046 0.019 0.0031 0.1021 0.0101 0.0246 0.0078 0.0446 0.0104 0.0373 0.0157
Golgi 0.0047 0.0005 0.0003 0.0042 0.0009 0.002 0.0176 0.0002 0.0009 0 0.0016 0.001 0.0099 0.0003 0.0011 0.0011 0.0003 0.0006
Lipid Particles 0.0078 0.0023 0.0013 0.0073 0.0028 0.0041 0.0485 0.0006 0.003 0.0001 0.0287 0.0033 0.0324 0.0013 0.0028 0.0018 0.0008 0.0064
Mitochondria 0.0095 0.0014 0.0016 0.0027 0.001 0.0005 0.0031 0.0005 0.0017 0.0001 0.0028 0.0069 0.0041 0.0003 0.0035 0.0078 0.0005 0.0003
None 0.7675 0.8456 0.8694 0.7102 0.8401 0.7986 0.398 0.6959 0.7977 0.87 0.4158 0.6949 0.6296 0.7918 0.7543 0.7119 0.8085 0.7507
Nuclear Periphery 0.0137 0.0008 0.0006 0.0098 0.0039 0.0026 0.0029 0.0004 0.0006 0.0003 0.0045 0.0004 0.0031 0.0007 0.0017 0.0024 0.0008 0.0005
Nucleolus 0.0009 0.0009 0.0004 0.0012 0.0016 0.0003 0.0005 0.0003 0.0012 0.0001 0.0005 0.0002 0.0003 0.0003 0.0004 0.0004 0.0002 0.0006
Nucleus 0.0035 0.0018 0.0008 0.0039 0.0041 0.0008 0.0008 0.0006 0.0016 0.0009 0.0013 0.0006 0.001 0.0005 0.001 0.0014 0.0006 0.0006
Peroxisomes 0.0092 0.0004 0.0005 0.0018 0.0005 0.0013 0.0397 0.0001 0.0007 0 0.0007 0.012 0.0257 0.0001 0.0004 0.0004 0.0002 0.0019
Punctate Nuclear 0.0222 0.0014 0.0004 0.0475 0.0404 0.0028 0.0029 0.0001 0.0004 0.0001 0.0009 0.0002 0.0024 0.0006 0.0007 0.0006 0.0004 0.0017
Vacuole 0.0137 0.033 0.0175 0.0432 0.0318 0.0144 0.0568 0.0156 0.0505 0.0051 0.0197 0.0814 0.0116 0.0039 0.0451 0.0088 0.0197 0.0287
Vacuole Periphery 0.0045 0.0037 0.0015 0.007 0.0059 0.0005 0.0018 0.001 0.0018 0.0004 0.0023 0.0091 0.0007 0.0003 0.0037 0.001 0.0017 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –
Standard name
Human Ortholog
Description Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Bio5

Bio5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bio5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available