ORF | |
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Human Ortholog | |
Description | Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 3 | – | 2 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 43 | 116 | 143 | 149 | 111 | 337 | 355 | 393 | – | 395 | 377 | 438 | 103 | 205 | 325 | 46 | 41 | 28 | 4 | 8 | 16 | 5 | 6 | 2 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | – | 3 | 0 | 0 | 3 | 5 | 2 | 9 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 2 |
Endosome | 6 | 6 | 21 | 1 | 3 | 0 | 0 | 3 | – | 1 | 0 | 1 | 12 | 1 | 0 | 5 | 1 | 5 | 8 | 12 | 9 | 4 | 7 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 0 | 0 | 1 | 2 |
Mitochondria | 20 | 13 | 40 | 11 | 46 | 62 | 69 | 84 | – | 103 | 100 | 112 | 2 | 0 | 1 | 4 | 3 | 4 | 5 | 5 | 7 | 2 | 6 | 5 |
Nucleus | 35 | 31 | 6 | 30 | 35 | 35 | 20 | 9 | – | 3 | 6 | 7 | 17 | 7 | 5 | 55 | 22 | 35 | 6 | 4 | 1 | 2 | 1 | 1 |
Nuclear Periphery | 2 | 0 | 0 | 5 | 5 | 0 | 20 | 2 | – | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 3 | 2 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 5 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 |
Vac/Vac Membrane | 209 | 314 | 190 | 170 | 206 | 144 | 92 | 75 | – | 99 | 72 | 68 | 141 | 67 | 22 | 108 | 83 | 68 | 261 | 368 | 302 | 150 | 159 | 157 |
Unique Cell Count | 268 | 393 | 330 | 259 | 300 | 457 | 461 | 475 | 496 | 470 | 517 | 243 | 257 | 340 | 181 | 129 | 123 | 299 | 407 | 349 | 178 | 197 | 189 | |
Labelled Cell Count | 315 | 482 | 407 | 366 | 407 | 583 | 563 | 569 | 612 | 565 | 634 | 279 | 286 | 357 | 230 | 155 | 144 | 299 | 407 | 349 | 178 | 197 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.3 | 4.6 | 4.6 | 4.4 | 4.0 | 3.6 | 3.7 | – | 3.5 | 3.6 | 3.6 | 5.6 | 4.9 | 4.9 | 6.5 | 6.6 | 6.5 | 6.7 | 6.6 | 6.7 |
Std Deviation (1e-4) | 1.1 | 1.0 | 1.4 | 1.3 | 1.3 | 1.0 | 1.1 | 1.2 | – | 1.0 | 1.1 | 1.0 | 1.0 | 0.8 | 0.8 | 1.4 | 1.5 | 1.7 | 1.4 | 1.8 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.05 | -0.2 | -0.35 | -0.28 | – | -0.4 | -0.35 | -0.35 | 0.3 | 0.12 | 0.12 | 0.51 | 0.52 | 0.52 | 0.55 | 0.53 | 0.57 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.4 | -1.6 | 8.6 | 9.7 | 11.7 | – | 10.7 | 10.8 | 12.7 | -0.2 | 8.9 | 14.7 | -4.0 | -2.3 | -4.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -0.7 | -3.8 | -4.7 | -1.8 | -2.5 | -0.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -5.1 | -5.8 | -6.4 | -3.8 | -3.2 | -2.8 |
Nucleus | 4.9 | 5.0 | 3.6 | 2.0 | 0.1 | – | 0 | 0 | -0.5 | 3.1 | 0.7 | -0.4 | 9.5 | 6.1 | 8.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.0 | 2.9 | -7.3 | -10.9 | -12.4 | – | -11.1 | -12.5 | -13.7 | 0.1 | -7.6 | -14.2 | 0.5 | 1.3 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3221 | 1.8139 | 1.2542 | 0.8722 | 0.8937 | 1.1128 | 2.5978 | 3.5457 | 3.4161 | 3.1666 | 2.7231 | 3.0276 | 0.0474 | 1.6618 | 0.7784 | 0.9613 | 1.0794 | 1.0344 |
Actin | 0.0056 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.016 | 0.0346 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Bud | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0011 | 0.0003 | 0.0002 | 0.0002 | 0.0004 |
Bud Neck | 0.0039 | 0.0013 | 0.0009 | 0.0017 | 0.0012 | 0.0013 | 0.0075 | 0.0009 | 0.013 | 0.0078 | 0.0005 | 0.0012 | 0.0004 | 0.0009 | 0.0008 | 0.0008 | 0.001 | 0.0013 |
Bud Periphery | 0.0013 | 0.0012 | 0.001 | 0.0008 | 0.0009 | 0.0013 | 0.0009 | 0.001 | 0.001 | 0.0011 | 0.0005 | 0.0006 | 0.0005 | 0.0028 | 0.001 | 0.0005 | 0.001 | 0.0013 |
Bud Site | 0.0025 | 0.004 | 0.001 | 0.0021 | 0.001 | 0.0008 | 0.0031 | 0.0011 | 0.0048 | 0.0128 | 0.0007 | 0.0007 | 0.0005 | 0.0021 | 0.0009 | 0.0007 | 0.0005 | 0.0007 |
Cell Periphery | 0.0015 | 0.0022 | 0.0016 | 0.0025 | 0.0011 | 0.002 | 0.0022 | 0.0023 | 0.0014 | 0.0016 | 0.0008 | 0.0017 | 0.0013 | 0.0024 | 0.0017 | 0.0018 | 0.002 | 0.002 |
Cytoplasm | 0.0237 | 0.0322 | 0.0297 | 0.0068 | 0.0056 | 0.0064 | 0.0082 | 0.0185 | 0.0057 | 0.0041 | 0.0055 | 0.0102 | 0.0084 | 0.0264 | 0.0098 | 0.0037 | 0.0022 | 0.011 |
Cytoplasmic Foci | 0.0197 | 0.0134 | 0.0226 | 0.0102 | 0.0241 | 0.0107 | 0.0166 | 0.0075 | 0.0254 | 0.0283 | 0.0086 | 0.006 | 0.0164 | 0.0077 | 0.0099 | 0.0033 | 0.0076 | 0.0073 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0052 | 0.0039 | 0.0042 | 0.0023 | 0.0009 | 0.0018 | 0.027 | 0.005 | 0.0033 | 0.0008 | 0.001 | 0.0023 | 0.0025 | 0.0056 | 0.0032 | 0.0006 | 0.0012 | 0.0036 |
Endosome | 0.2537 | 0.1616 | 0.2147 | 0.2489 | 0.1665 | 0.1497 | 0.2464 | 0.1657 | 0.1996 | 0.2565 | 0.2706 | 0.1711 | 0.2192 | 0.1159 | 0.1792 | 0.1365 | 0.1785 | 0.1619 |
Golgi | 0.0045 | 0.0036 | 0.0056 | 0.0028 | 0.0029 | 0.0026 | 0.0056 | 0.0043 | 0.0086 | 0.0071 | 0.0037 | 0.0036 | 0.0037 | 0.0014 | 0.0033 | 0.0014 | 0.0025 | 0.0022 |
Lipid Particles | 0.0172 | 0.0093 | 0.023 | 0.0195 | 0.0465 | 0.0096 | 0.0144 | 0.0084 | 0.0188 | 0.0105 | 0.0022 | 0.0052 | 0.0194 | 0.0061 | 0.0135 | 0.0015 | 0.0103 | 0.0058 |
Mitochondria | 0.0161 | 0.0155 | 0.0616 | 0.0372 | 0.0014 | 0.0082 | 0.0034 | 0.0108 | 0.0075 | 0.0203 | 0.0022 | 0.0067 | 0.0127 | 0.0038 | 0.0184 | 0.0005 | 0.0063 | 0.0106 |
None | 0.0991 | 0.0281 | 0.0536 | 0.0048 | 0.0226 | 0.01 | 0.0355 | 0.0498 | 0.0016 | 0.001 | 0.0002 | 0.0017 | 0.0117 | 0.0135 | 0.0127 | 0.0002 | 0.0012 | 0.0082 |
Nuclear Periphery | 0.0089 | 0.0064 | 0.0098 | 0.0054 | 0.0035 | 0.0046 | 0.0082 | 0.0073 | 0.005 | 0.0013 | 0.0019 | 0.0035 | 0.0054 | 0.0085 | 0.0089 | 0.0013 | 0.0018 | 0.0063 |
Nucleolus | 0.0078 | 0.0059 | 0.0062 | 0.0159 | 0.0141 | 0.0052 | 0.0054 | 0.0021 | 0.0074 | 0.0025 | 0.0009 | 0.0019 | 0.0025 | 0.0024 | 0.0053 | 0.0022 | 0.007 | 0.0044 |
Nucleus | 0.0106 | 0.0096 | 0.0128 | 0.0102 | 0.0045 | 0.0096 | 0.0137 | 0.0117 | 0.0047 | 0.0022 | 0.0019 | 0.0074 | 0.0032 | 0.0059 | 0.0116 | 0.0026 | 0.0034 | 0.0085 |
Peroxisomes | 0.0013 | 0.0011 | 0.002 | 0.0035 | 0.0012 | 0.0011 | 0.0012 | 0.0013 | 0.0074 | 0.0072 | 0.0004 | 0.0007 | 0.0014 | 0.0004 | 0.0011 | 0.0003 | 0.0012 | 0.0007 |
Punctate Nuclear | 0.0042 | 0.0005 | 0.0008 | 0.0006 | 0.0087 | 0.0006 | 0.0011 | 0.0002 | 0.0024 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0019 | 0 | 0.0001 | 0.0003 |
Vacuole | 0.4254 | 0.5928 | 0.4533 | 0.519 | 0.5983 | 0.6397 | 0.4938 | 0.579 | 0.5232 | 0.5356 | 0.5655 | 0.6112 | 0.518 | 0.6356 | 0.5618 | 0.7412 | 0.649 | 0.6134 |
Vacuole Periphery | 0.0869 | 0.1067 | 0.095 | 0.1058 | 0.0945 | 0.1344 | 0.1054 | 0.1227 | 0.1424 | 0.0625 | 0.1326 | 0.1637 | 0.1719 | 0.1571 | 0.1545 | 0.1006 | 0.123 | 0.1501 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.0216 | 32.8792 | 31.0517 | 40.3284 | 45.5195 | 50.127 | 38.9107 | 29.0668 | 39.3956 | 47.2855 |
Translational Efficiency | 1.1747 | 1.6343 | 1.5471 | 1.2294 | 1.168 | 1.4266 | 1.3548 | 1.2339 | 1.053 | 1.0698 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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