Standard name
Human Ortholog
Description Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0 0.1 0.08 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.91 0.92 0.97 0.88 0.81 0.85 0.92 0.96 0.94 0.94 0.91 0.96 0.97 0.99 0.87 0.85 0.86 0.23 0.34 0.3 0.39 0.4 0.56
Endoplasmic Reticulum 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.05 0.2 0.15 0.09 0.06 0.1 0.12 0.17 0 0 0 0 0 0 0.06 0 0.17 0.06 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0.13 0.17 0.14 0.41 0.37 0.28 0.23 0.22 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 2 0 1 2 0 2 1 0 1 0 0 7 2 0 1 0 0 2 13 4 8 20 11
Bud 1 4 3 0 5 3 4 6 5 3 4 4 3 1 0 0 1 0 3 15 1 11 10 7
Bud Neck 0 4 4 1 0 0 1 6 1 1 1 2 3 1 0 3 3 0 9 28 5 14 22 12
Bud Site 1 1 1 0 1 0 0 1 0 1 0 0 2 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 1 3 1 0 0 2 0 0 1 0 1 1 2 3 1 4 0 3 5 2 5 6
Cytoplasm 62 272 141 118 143 113 155 195 137 182 147 163 325 222 240 413 321 272 21 112 41 54 110 164
Endoplasmic Reticulum 7 6 4 0 4 2 3 0 1 0 0 0 2 0 1 33 14 20 2 3 1 1 10 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 6 2 5 5 8 4 1 5 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 2 1 1 0 2 1
Mitochondria 1 6 6 6 8 28 28 20 8 20 18 30 1 0 1 10 1 7 5 12 24 8 18 13
Nucleus 0 0 0 1 3 2 5 3 0 1 1 0 0 1 0 11 4 5 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 2 2 0 0 1 0 0 0 0 1 0 3 0 0 3 5 6 0 2 2 0 3 1
Vac/Vac Membrane 0 14 0 1 8 8 8 5 2 3 2 5 1 0 0 64 63 44 39 122 39 31 61 61
Unique Cell Count 73 299 153 122 162 140 182 211 142 193 156 179 338 228 242 475 377 317 95 333 140 138 278 294
Labelled Cell Count 78 316 163 131 175 157 206 240 154 213 175 204 350 232 244 550 419 364 95 333 140 138 278 294


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.1 3.1 3.1 3.5 3.3 3.2 3.4 3.3 2.8 3.0 2.6 4.3 4.2 3.8 6.3 6.7 6.6 4.4 4.4 5.0
Std Deviation (1e-4) 1.1 0.8 0.6 0.9 1.2 0.8 1.0 1.1 1.8 1.3 1.2 0.8 0.9 0.9 0.9 1.7 2.2 2.5 1.0 1.3 1.4
Intensity Change (Log2) – – – -0.02 0.19 0.1 0.04 0.11 0.07 -0.12 -0.06 -0.23 0.46 0.44 0.28 1.01 1.11 1.09 0.49 0.51 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.8 5.4 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2178 0.1035 -0.2639 -0.3198 -0.4812 0.3327 1.9714 1.9879 1.3652 1.8043 2.6201 1.7737 2.8496 2.704 2.6431 2.8858 2.5133 2.913
Actin 0.0724 0.0015 0.0001 0.0198 0.0163 0.0189 0.0281 0.0003 0.0037 0.0003 0.0305 0.0023 0 0.001 0 0.0014 0.0003 0.0001
Bud 0.0037 0.001 0.0015 0.0062 0.0004 0.0005 0.0007 0.0029 0.0025 0.0003 0.0011 0.001 0.0001 0.0075 0.0001 0.0002 0.0012 0.0002
Bud Neck 0.0186 0.0004 0.0003 0.0024 0.0007 0.0014 0.0022 0.0001 0.0069 0.0001 0.0039 0.0009 0.0004 0.0003 0 0.0001 0.0001 0.0117
Bud Periphery 0.0074 0.0017 0.0017 0.0228 0.0007 0.0012 0.0014 0.0032 0.0029 0.0006 0.0013 0.0013 0.0002 0.0089 0.0001 0.0003 0.001 0.0002
Bud Site 0.0148 0.0054 0.0003 0.0024 0.0009 0.0006 0.0062 0.0019 0.0045 0.0002 0.0054 0.0005 0.0007 0.0079 0.0001 0.0002 0.0001 0.0001
Cell Periphery 0.0034 0.001 0.0003 0.001 0.0002 0.0002 0.0006 0.0004 0.0005 0.0003 0.0003 0.0002 0.001 0.0008 0.0001 0.0001 0.0001 0.0002
Cytoplasm 0.155 0.2425 0.2534 0.153 0.3082 0.3337 0.1117 0.1169 0.1234 0.1554 0.1294 0.2315 0.0865 0.0813 0.0646 0.1452 0.0774 0.184
Cytoplasmic Foci 0.0628 0.0208 0.0382 0.0678 0.0177 0.0666 0.0311 0.0235 0.0214 0.0241 0.0181 0.0354 0.0241 0.0226 0.0081 0.0296 0.0052 0.0755
Eisosomes 0.0013 0.0002 0.0001 0.0004 0.0001 0.0003 0.0006 0.0001 0.0002 0 0.0004 0.0001 0.0001 0.0002 0.0001 0.0006 0.0003 0.0001
Endoplasmic Reticulum 0.0061 0.0045 0.0047 0.0064 0.0016 0.0045 0.0049 0.0019 0.0024 0.0013 0.0168 0.0018 0.0017 0.0017 0.0009 0.0016 0.0004 0.0022
Endosome 0.0541 0.0138 0.038 0.0483 0.0189 0.0571 0.0365 0.0166 0.0241 0.028 0.0803 0.0112 0.0289 0.0078 0.008 0.0252 0.0002 0.0571
Golgi 0.0169 0.0009 0.0007 0.006 0.0098 0.0089 0.0033 0.0004 0.0016 0.0019 0.0155 0.0014 0.0005 0.0006 0.0002 0.0006 0.0001 0.0014
Lipid Particles 0.036 0.006 0.0088 0.0096 0.0112 0.0129 0.0076 0.0021 0.0053 0.0337 0.015 0.0017 0.0026 0.0027 0.0004 0.0012 0.0003 0.0027
Mitochondria 0.0377 0.0009 0.0009 0.0202 0.0081 0.0157 0.0029 0.0007 0.0014 0.0014 0.0058 0.0013 0.001 0.0017 0.0003 0.0016 0.0003 0.0017
None 0.4133 0.6767 0.6351 0.5465 0.5663 0.4309 0.6823 0.8197 0.7818 0.6913 0.5691 0.6931 0.8077 0.8232 0.9065 0.772 0.9019 0.6441
Nuclear Periphery 0.0168 0.0011 0.0012 0.0084 0.0015 0.0126 0.0182 0.0006 0.001 0.003 0.0516 0.0019 0.0004 0.0006 0.0004 0.0009 0.0008 0.0006
Nucleolus 0.004 0.0005 0.0004 0.0006 0.0003 0.0009 0.0021 0.0003 0.0005 0.0016 0.0016 0.0007 0.0006 0.0017 0.0001 0.0003 0.0006 0.0003
Nucleus 0.0053 0.0016 0.0015 0.0026 0.001 0.0047 0.0148 0.0008 0.0012 0.0349 0.0209 0.002 0.0009 0.0023 0.0006 0.0009 0.0022 0.001
Peroxisomes 0.0261 0.0059 0.0006 0.0056 0.0031 0.0034 0.0077 0.0003 0.0016 0.0036 0.0045 0.0041 0.0002 0.0013 0.0001 0.001 0.0001 0.0015
Punctate Nuclear 0.0057 0.0009 0.0016 0.0044 0.0012 0.01 0.0222 0.0005 0.0019 0.0079 0.0134 0.0026 0.0003 0.0014 0.0002 0.0006 0.0069 0.0007
Vacuole 0.0347 0.0122 0.0093 0.0404 0.0294 0.0124 0.0128 0.0062 0.0103 0.0091 0.0104 0.0043 0.0412 0.0218 0.0081 0.0141 0.0005 0.0129
Vacuole Periphery 0.0039 0.0007 0.0013 0.0254 0.0024 0.0024 0.002 0.0005 0.001 0.0011 0.0048 0.0007 0.001 0.0026 0.0009 0.0021 0.0001 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.7572 5.8818 42.9907 191.9225 81.8955 2.6304 6.9135 10.3357 93.8654 161.3306
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother
Localization
Cell Percentages cytoplasm (26%), vacuole (3%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dse4

Dse4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dse4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available