Standard name | |
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Human Ortholog | |
Description | Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 4 | 7 | 7 | 10 | 11 | 7 | 12 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 |
Bud | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 3 | 2 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 8 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | 15 | 6 | 7 | 9 | 18 | 24 | 35 | 28 | 28 | 33 | 12 | 14 | 16 | 11 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 260 | 149 | 260 | 157 | 164 | 227 | 252 | 190 | 195 | 156 | 167 | 183 | 263 | 437 | 462 | 260 | 272 | 214 | 245 | 147 | 250 | 217 | 403 | 316 |
Endoplasmic Reticulum | 4 | 2 | 8 | 5 | 8 | 13 | 35 | 40 | 37 | 30 | 20 | 30 | 1 | 3 | 7 | 7 | 5 | 5 | 5 | 0 | 9 | 1 | 5 | 6 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 4 | 3 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 2 | 1 | 1 | 0 |
Mitochondria | 3 | 1 | 4 | 13 | 7 | 25 | 80 | 71 | 58 | 96 | 90 | 88 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 4 | 7 | 3 | 4 | 17 | 9 | 28 | 14 | 37 | 0 | 5 | 4 | 16 | 10 | 4 | 1 | 2 | 1 | 1 | 3 | 1 |
Unique Cell Count | 266 | 151 | 268 | 172 | 182 | 261 | 352 | 306 | 299 | 290 | 276 | 320 | 264 | 442 | 472 | 278 | 284 | 223 | 257 | 155 | 274 | 228 | 438 | 339 |
Labelled Cell Count | 274 | 153 | 288 | 186 | 194 | 281 | 400 | 361 | 349 | 355 | 335 | 392 | 277 | 461 | 492 | 298 | 300 | 232 | 257 | 155 | 274 | 228 | 438 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.5 | 14.9 | 13.3 | 12.7 | 14.3 | 13.4 | 13.2 | 13.7 | 13.0 | 12.9 | 12.3 | 13.4 | 14.7 | 14.4 | 14.8 | 23.6 | 22.2 | 23.3 | 14.0 | 17.0 | 20.3 |
Std Deviation (1e-4) | 1.9 | 2.8 | 3.5 | 3.6 | 3.5 | 2.8 | 2.9 | 3.4 | 3.3 | 3.4 | 3.3 | 3.8 | 2.9 | 2.8 | 3.1 | 6.9 | 6.3 | 6.9 | 3.0 | 4.9 | 6.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.1 | 0.01 | -0.02 | 0.03 | -0.04 | -0.05 | -0.12 | 0.01 | 0.14 | 0.11 | 0.15 | 0.82 | 0.73 | 0.81 | 0.07 | 0.35 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.8 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -0.8 | -1.2 | -0.3 | 1.1 | 2.6 | 1.8 | 2.0 | 2.1 | -0.6 | -1.6 | -1.4 | -0.9 | -1.4 | -1.9 |
Cytoplasm | -2.6 | -3.1 | -4.3 | -8.3 | -10.1 | -9.5 | -11.7 | -10.4 | -11.2 | 2.3 | 1.8 | 0.7 | -1.9 | -0.8 | -0.6 |
Endoplasmic Reticulum | 0 | 0.8 | 1.2 | 3.4 | 4.4 | 4.1 | 3.4 | 2.2 | 3.1 | 0 | 0 | -1.4 | -0.3 | -0.9 | -0.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 0 | 4.1 | 7.7 | 7.7 | 6.8 | 9.7 | 9.6 | 8.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.9 | 0 | 5.2 | 3.7 | 5.8 | 0 | 0 | 0 | 4.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.3836 | 10.8612 | 10.452 | 10.3225 | 9.5085 | 10.5367 | 8.6515 | 12.1373 | 10.3972 | 9.4961 | 10.2273 | 10.2544 | 6.5903 | 9.8384 | 10.199 | 5.2507 | 5.4792 | 9.1229 |
Actin | 0.0044 | 0.0005 | 0.0031 | 0.0003 | 0.0229 | 0.002 | 0.0176 | 0.0002 | 0.0104 | 0.0016 | 0.0017 | 0.0018 | 0.0278 | 0.0008 | 0.0006 | 0.1486 | 0.035 | 0.0028 |
Bud | 0.0005 | 0.0003 | 0.001 | 0.0008 | 0.0005 | 0.0003 | 0.0009 | 0.0018 | 0.0005 | 0.0011 | 0.0004 | 0.0004 | 0.0006 | 0.0029 | 0.0003 | 0.0015 | 0.0118 | 0.0002 |
Bud Neck | 0.0005 | 0.0002 | 0.0005 | 0.0006 | 0.0008 | 0.0009 | 0.0014 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0009 | 0.0005 | 0.0001 | 0.0003 | 0.0021 | 0.0031 | 0.0004 |
Bud Periphery | 0.0003 | 0.0001 | 0.001 | 0.0003 | 0.0009 | 0.0002 | 0.0008 | 0.0005 | 0.0004 | 0.0014 | 0.0003 | 0.0002 | 0.0006 | 0.0009 | 0.0002 | 0.0015 | 0.0077 | 0.0001 |
Bud Site | 0.001 | 0.0008 | 0.0032 | 0.0006 | 0.002 | 0.0002 | 0.0025 | 0.0008 | 0.0012 | 0.0006 | 0.0004 | 0.0002 | 0.0026 | 0.0009 | 0.0008 | 0.005 | 0.0895 | 0.0001 |
Cell Periphery | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.001 | 0.0001 |
Cytoplasm | 0.8718 | 0.9734 | 0.9377 | 0.9263 | 0.868 | 0.9336 | 0.7628 | 0.9688 | 0.904 | 0.8755 | 0.8777 | 0.9162 | 0.7671 | 0.9843 | 0.9362 | 0.4629 | 0.7331 | 0.8975 |
Cytoplasmic Foci | 0.0189 | 0.0051 | 0.01 | 0.0088 | 0.0209 | 0.0107 | 0.0313 | 0.005 | 0.0133 | 0.0103 | 0.0244 | 0.0078 | 0.0136 | 0.0016 | 0.0078 | 0.0703 | 0.0349 | 0.0058 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0007 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.045 | 0.0081 | 0.0086 | 0.0049 | 0.0129 | 0.0148 | 0.0445 | 0.0047 | 0.009 | 0.0152 | 0.0051 | 0.0263 | 0.0659 | 0.004 | 0.0056 | 0.0153 | 0.0023 | 0.0299 |
Endosome | 0.0253 | 0.0055 | 0.0176 | 0.032 | 0.0323 | 0.0178 | 0.0664 | 0.0047 | 0.0289 | 0.0381 | 0.0379 | 0.0234 | 0.0381 | 0.001 | 0.0272 | 0.109 | 0.0198 | 0.0272 |
Golgi | 0.0025 | 0.0006 | 0.0023 | 0.0011 | 0.0158 | 0.0033 | 0.0169 | 0.0005 | 0.0076 | 0.005 | 0.0056 | 0.0056 | 0.0134 | 0.0003 | 0.0045 | 0.0916 | 0.0289 | 0.0243 |
Lipid Particles | 0.0011 | 0 | 0.0003 | 0.0002 | 0.0038 | 0.0037 | 0.0068 | 0.0001 | 0.0021 | 0.0092 | 0.0065 | 0.0023 | 0.0111 | 0 | 0.0006 | 0.0257 | 0.0014 | 0.0008 |
Mitochondria | 0.0012 | 0.0002 | 0.0009 | 0.0003 | 0.004 | 0.0018 | 0.0064 | 0.0002 | 0.0018 | 0.0072 | 0.0157 | 0.0034 | 0.0151 | 0.0001 | 0.001 | 0.0281 | 0.0124 | 0.0058 |
None | 0.0059 | 0.0013 | 0.0016 | 0.0008 | 0.0028 | 0.0013 | 0.0031 | 0.0071 | 0.0009 | 0.0012 | 0.0021 | 0.0016 | 0.0075 | 0.0012 | 0.0009 | 0.0011 | 0.0008 | 0.0006 |
Nuclear Periphery | 0.0037 | 0.0005 | 0.001 | 0.0012 | 0.0026 | 0.0009 | 0.0116 | 0.0006 | 0.0038 | 0.0041 | 0.0017 | 0.0027 | 0.01 | 0.0004 | 0.0016 | 0.0017 | 0.0006 | 0.001 |
Nucleolus | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0025 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Nucleus | 0.0036 | 0.0008 | 0.0012 | 0.0012 | 0.0011 | 0.0007 | 0.0053 | 0.0008 | 0.0024 | 0.002 | 0.0017 | 0.0015 | 0.0029 | 0.0005 | 0.0009 | 0.0008 | 0.0012 | 0.0007 |
Peroxisomes | 0.0007 | 0 | 0.0005 | 0 | 0.0029 | 0.0047 | 0.0052 | 0.0001 | 0.0025 | 0.0018 | 0.0029 | 0.0003 | 0.0031 | 0 | 0.0001 | 0.0273 | 0.0034 | 0.0001 |
Punctate Nuclear | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0.0027 | 0.0001 | 0.0007 | 0.0019 | 0.0011 | 0.0011 | 0.0095 | 0 | 0.0001 | 0.0014 | 0.0004 | 0.0001 |
Vacuole | 0.0092 | 0.0023 | 0.0077 | 0.0172 | 0.0029 | 0.0016 | 0.0109 | 0.0034 | 0.0085 | 0.0194 | 0.012 | 0.0033 | 0.0055 | 0.0006 | 0.0094 | 0.0032 | 0.0111 | 0.0013 |
Vacuole Periphery | 0.0013 | 0.0003 | 0.001 | 0.0031 | 0.001 | 0.0006 | 0.0021 | 0.0004 | 0.0013 | 0.0037 | 0.0019 | 0.0009 | 0.0017 | 0.0001 | 0.0015 | 0.0015 | 0.0012 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.2191 | 16.146 | 14.704 | 29.2452 | 17.5633 | 32.8212 | 30.0533 | 30.5511 | 44.7051 | 32.4739 |
Translational Efficiency | 2.0263 | 1.8433 | 1.7758 | 1.2342 | 1.6742 | 1.8864 | 1.3319 | 1.2118 | 0.9742 | 1.1756 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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