Standard name
Human Ortholog
Description Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0 0 0 0.05 0.08 0.12 0.1 0.1 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.91 0.9 0.87 0.72 0.62 0.65 0.54 0.61 0.57 1.0 0.99 0.98 0.94 0.96 0.96 0.95 0.95 0.92 0.96 0.92 0.93
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.1 0.13 0.12 0.1 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0.1 0.23 0.23 0.19 0.33 0.33 0.28 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.1 0.05 0.12 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 4 7 7 10 11 7 12 0 0 1 0 0 0 0 0 1 0 3 0
Bud 0 1 0 1 0 0 2 3 2 4 4 4 0 0 0 0 0 0 0 1 1 1 8 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6 0 15 6 7 9 18 24 35 28 28 33 12 14 16 11 9 5 0 0 0 0 0 0
Cytoplasm 260 149 260 157 164 227 252 190 195 156 167 183 263 437 462 260 272 214 245 147 250 217 403 316
Endoplasmic Reticulum 4 2 8 5 8 13 35 40 37 30 20 30 1 3 7 7 5 5 5 0 9 1 5 6
Endosome 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 3 0 0 4 3 0 0 1 2 0 2 0 0 2 1 1 0
Mitochondria 3 1 4 13 7 25 80 71 58 96 90 88 0 0 0 2 3 1 0 0 2 0 1 0
Nucleus 0 0 0 0 0 0 2 1 3 2 0 0 1 2 0 0 1 1 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 4 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 4 7 3 4 17 9 28 14 37 0 5 4 16 10 4 1 2 1 1 3 1
Unique Cell Count 266 151 268 172 182 261 352 306 299 290 276 320 264 442 472 278 284 223 257 155 274 228 438 339
Labelled Cell Count 274 153 288 186 194 281 400 361 349 355 335 392 277 461 492 298 300 232 257 155 274 228 438 339


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 14.9 13.3 12.7 14.3 13.4 13.2 13.7 13.0 12.9 12.3 13.4 14.7 14.4 14.8 23.6 22.2 23.3 14.0 17.0 20.3
Std Deviation (1e-4) 1.9 2.8 3.5 3.6 3.5 2.8 2.9 3.4 3.3 3.4 3.3 3.8 2.9 2.8 3.1 6.9 6.3 6.9 3.0 4.9 6.6
Intensity Change (Log2) -0.07 0.1 0.01 -0.02 0.03 -0.04 -0.05 -0.12 0.01 0.14 0.11 0.15 0.82 0.73 0.81 0.07 0.35 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 2.6 2.8 0 2.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -0.8 -1.2 -0.3 1.1 2.6 1.8 2.0 2.1 -0.6 -1.6 -1.4 -0.9 -1.4 -1.9
Cytoplasm -2.6 -3.1 -4.3 -8.3 -10.1 -9.5 -11.7 -10.4 -11.2 2.3 1.8 0.7 -1.9 -0.8 -0.6
Endoplasmic Reticulum 0 0.8 1.2 3.4 4.4 4.1 3.4 2.2 3.1 0 0 -1.4 -0.3 -0.9 -0.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 0 4.1 7.7 7.7 6.8 9.7 9.6 8.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.9 0 5.2 3.7 5.8 0 0 0 4.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.3836 10.8612 10.452 10.3225 9.5085 10.5367 8.6515 12.1373 10.3972 9.4961 10.2273 10.2544 6.5903 9.8384 10.199 5.2507 5.4792 9.1229
Actin 0.0044 0.0005 0.0031 0.0003 0.0229 0.002 0.0176 0.0002 0.0104 0.0016 0.0017 0.0018 0.0278 0.0008 0.0006 0.1486 0.035 0.0028
Bud 0.0005 0.0003 0.001 0.0008 0.0005 0.0003 0.0009 0.0018 0.0005 0.0011 0.0004 0.0004 0.0006 0.0029 0.0003 0.0015 0.0118 0.0002
Bud Neck 0.0005 0.0002 0.0005 0.0006 0.0008 0.0009 0.0014 0.0002 0.0005 0.0005 0.0004 0.0009 0.0005 0.0001 0.0003 0.0021 0.0031 0.0004
Bud Periphery 0.0003 0.0001 0.001 0.0003 0.0009 0.0002 0.0008 0.0005 0.0004 0.0014 0.0003 0.0002 0.0006 0.0009 0.0002 0.0015 0.0077 0.0001
Bud Site 0.001 0.0008 0.0032 0.0006 0.002 0.0002 0.0025 0.0008 0.0012 0.0006 0.0004 0.0002 0.0026 0.0009 0.0008 0.005 0.0895 0.0001
Cell Periphery 0.0008 0.0002 0.0004 0.0002 0.001 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0002 0.0003 0.0003 0.001 0.0001
Cytoplasm 0.8718 0.9734 0.9377 0.9263 0.868 0.9336 0.7628 0.9688 0.904 0.8755 0.8777 0.9162 0.7671 0.9843 0.9362 0.4629 0.7331 0.8975
Cytoplasmic Foci 0.0189 0.0051 0.01 0.0088 0.0209 0.0107 0.0313 0.005 0.0133 0.0103 0.0244 0.0078 0.0136 0.0016 0.0078 0.0703 0.0349 0.0058
Eisosomes 0 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0.0004 0 0 0.0007 0.0001 0
Endoplasmic Reticulum 0.045 0.0081 0.0086 0.0049 0.0129 0.0148 0.0445 0.0047 0.009 0.0152 0.0051 0.0263 0.0659 0.004 0.0056 0.0153 0.0023 0.0299
Endosome 0.0253 0.0055 0.0176 0.032 0.0323 0.0178 0.0664 0.0047 0.0289 0.0381 0.0379 0.0234 0.0381 0.001 0.0272 0.109 0.0198 0.0272
Golgi 0.0025 0.0006 0.0023 0.0011 0.0158 0.0033 0.0169 0.0005 0.0076 0.005 0.0056 0.0056 0.0134 0.0003 0.0045 0.0916 0.0289 0.0243
Lipid Particles 0.0011 0 0.0003 0.0002 0.0038 0.0037 0.0068 0.0001 0.0021 0.0092 0.0065 0.0023 0.0111 0 0.0006 0.0257 0.0014 0.0008
Mitochondria 0.0012 0.0002 0.0009 0.0003 0.004 0.0018 0.0064 0.0002 0.0018 0.0072 0.0157 0.0034 0.0151 0.0001 0.001 0.0281 0.0124 0.0058
None 0.0059 0.0013 0.0016 0.0008 0.0028 0.0013 0.0031 0.0071 0.0009 0.0012 0.0021 0.0016 0.0075 0.0012 0.0009 0.0011 0.0008 0.0006
Nuclear Periphery 0.0037 0.0005 0.001 0.0012 0.0026 0.0009 0.0116 0.0006 0.0038 0.0041 0.0017 0.0027 0.01 0.0004 0.0016 0.0017 0.0006 0.001
Nucleolus 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0.0002 0.0002 0.0001 0.0025 0 0 0.0002 0.0002 0
Nucleus 0.0036 0.0008 0.0012 0.0012 0.0011 0.0007 0.0053 0.0008 0.0024 0.002 0.0017 0.0015 0.0029 0.0005 0.0009 0.0008 0.0012 0.0007
Peroxisomes 0.0007 0 0.0005 0 0.0029 0.0047 0.0052 0.0001 0.0025 0.0018 0.0029 0.0003 0.0031 0 0.0001 0.0273 0.0034 0.0001
Punctate Nuclear 0.0023 0.0001 0.0002 0.0001 0.0004 0.0007 0.0027 0.0001 0.0007 0.0019 0.0011 0.0011 0.0095 0 0.0001 0.0014 0.0004 0.0001
Vacuole 0.0092 0.0023 0.0077 0.0172 0.0029 0.0016 0.0109 0.0034 0.0085 0.0194 0.012 0.0033 0.0055 0.0006 0.0094 0.0032 0.0111 0.0013
Vacuole Periphery 0.0013 0.0003 0.001 0.0031 0.001 0.0006 0.0021 0.0004 0.0013 0.0037 0.0019 0.0009 0.0017 0.0001 0.0015 0.0015 0.0012 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.2191 16.146 14.704 29.2452 17.5633 32.8212 30.0533 30.5511 44.7051 32.4739
Translational Efficiency 2.0263 1.8433 1.7758 1.2342 1.6742 1.8864 1.3319 1.2118 0.9742 1.1756

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Cmk2

Cmk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cmk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available